Tnks2 (NM_001163635) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5430432P15Rik; AA517131; AI662480; ARTD6; Tank2; TNKS-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215283 representing NM_001163635 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCCGCCGCTGCGCCGGCGGGGGCGCGGCCTGCGCGAGCGCCGGGGCCGAGGCCGTGGAGCCGT CCGCCCGCGAGCTGTTCGAGGCTTGCCGCAACGGGGACGTGGAGCGAGTCAAGAGGCTGGTGACGCCCGA GAAGGTGAACAGCCGCGACACGGCGGGCAGGAAGTCCACCCCGCTCCACTTCGCCGCAGGTTTTGGACGG AAGGATGTAGTTGAATATCTTCTTCAGAACGGTGCAAATGTGCAAGCGCGTGATGATGGGGGTCTTATTC CTCTTCATAACGCATGCTCTTTTGGTCATGCTGAAGTAGTCAATCTTCTTTTACAACACGGTGCAGACCC AAATGCTCGGGATAATTGGAATTATACTCCTCTCCATGAAGCTGCAATTAAAGGAAAAATTGATGTTTGC ATTGTGCTTTTACAGCATGGAGCTGAGCCAACCATCCGAAATACAGATGGAAGGACAGCATTGGATTTAG CAGACCCATCTGCCAAAGCTGTTCTGACTGGTGACTATAAGAAAGATGAACTTCTGGAAAGTGCCAGGAG TGGCAATGAGGAAAAAATGATGGCCCTGCTTACACCATTGAATGTCAACTGCCATGCAAGTGATGGCAGA AAGTCAACTCCATTGCATTTGGCAGCAGGATACAACAGAGTAAAAATTGTACAACTGTTACTGCACCATG GGGCTGATGTCCATGCTAAAGATAAAGGGGATCTGGTACCACTACACAATGCCTGCTCTTACGGTCATTA TGAAGTAACTGAACTTCTGGTCAAGCACGGTGCCTGTGTGAATGCAATGGACCTGTGGCAGTTCACTCCT CTTCATGAGGCAGCTTCTAAGAACAGGATTGAAGTATGTTCTCTCCTCCTAAGTTACGGGGCAGATCCAA CCCTGCTGAACTGTCACAATAAAAGTGCTATAGACCTGGCCCCCACGGCACAGCTGAAGGAAAGATTATC GTATGAATTTAAAGGCCATTCATTGCTGCAAGCTGCACGAGAAGCTGATGTTACAAGAATCAAAAAACAT CTTTCTCTGGAAATGGTGAATTTCAAACATCCTCAGACACATGAAACAGCACTGCATTGTGCTGCTGCAT CTCCATATCCCAAAAGAAAGCAGATATGTGAACTGCTGCTCAGAAAGGGAGCAAACACCAACGAAAAGAC TAAAGAATTCTTGACTCCTCTGCACGTGGCTTCTGAGAACGCTCACAATGATGTTGTTGAAGTAGTGGTG AAACATGAAGCAAAGGTTAATGCTTTGGATAGTCTTGGACAGACGTCATTACACAGAGCTGCACACTGTG GTCACCTGCAGACCTGCCGCCTGCTCCTGAGCTATGGGTGTGATCCAAACATCATATCCCTTCAGGGTTT CACCGCCTTGCAAATGGGAAATGAAAATGTGCAGCAGCTGCTCCAAGAGGGCGCCTCACTAGGTCACTCA GAGGCGGACAGACAGCTGCTGGAAGCTGCAAAGGCTGGTGACGTTGAAACTGTAAAAAAACTCTGTACTG TTCAGAGTGTCAACTGCAGAGACATTGAAGGACGCCAGTCAACCCCACTCCACTTTGCAGCTGGGTACAA CAGAGTGTCTGTGGTAGAATATCTGCTGCAACATGGAGCTGATGTTCATGCTAAAGACAAAGGAGGTCTT GTACCTTTACACAATGCATGTTCTTATGGACACTATGAAGTTGCAGAACTTCTTGTCAAGCATGGAGCAG TAGTCAATGTCGCTGACTTGTGGAAGTTCACACCTTTACATGAAGCTGCAGCAAAAGGAAAATATGAAAT ATGCAAACTTCTACTCCAGCATGGTGCAGACCCTACAAAGAAAAACAGAGATGGTAATACTCCTCTGGAT CTTGTTAAAGATGGAGATACAGATATTCAAGATCTACTTAGAGGTGATGCAGCTTTGCTGGATGCTGCCA AGAAGGGTTGTTTAGCCAGAGTGAAGAAATTGTCTTCTCCTGATAATGTAAATTGCCGTGATACCCAAGG TCGGCATTCTACACCTTTACATTTGGCAGCTGGTTATAATAACTTGGAAGTTGCTGAGTATTTATTGCAA CATGGAGCTGATGTGAATGCCCAGGACAAAGGAGGACTCATTCCTTTACATAACGCAGCATCGTATGGAC ATGTAGATGTAGCAGCTTTGCTGATAAAGTACAATGCGTGTGTCAATGCCACGGACAAATGGGCTTTCAC ACCGTTACATGAAGCAGCCCAAAAGGGACGGACACAGCTTTGTGCTTTATTGTTGGCCCATGGAGCTGAT CCTACTCTTAAAAATCAAGAAGGACAAACACCTTTAGATTTAGTTTCAGCAGATGATGTCAGTGCACTCT TGACAGCAGCCATGCCCCCCTCTGCTCTGCCTACGTGCTACAAACCTCAAGTGCTCAGTGGCGTGAGGGG CCCCGGAGCCACTGCAGATGCTCTGTCTTCAGGTCCGTCCAGCCCATCCAGCCTCTCTGCAGCCAGCAGC CTCGACAACTTATCTGGCAGCTTCTCGGAACTGTCCGCAGTGGTTAGTTCAAGTGCAGCAGAAGGTGCTA CTGGTTTGCAAAGAAAAGAGGATTCAGGAATCGATTTTAGTATAACTCAGTTCATAAGGAACCTTGGACT TGAGCACTTGATGGATATATTTGAGAGAGAACAGATTACCTTAGATGTCCTAGTTGAAATGGGCCACAAG GAACTGAAAGAGATTGGAATCAATGCCTATGGACATCGACACAAGCTGATTAAAGGAGTTGAAAGGCTGA TCTCTGGACAACAAGGTCTTAATCCATATTTAACTCTAAACAACTCTGGTAGTGGAACAATTCTCATAGA TCTGTCTCCTGATGATAAAGAATTTCAGTCTGTGGAAGAAGAGATGCAGAGTACTGTGCGGGAGCACAGA GATGGTGGTCACGCAGGCGGCGTCTTCAACAGATACAACATTCTCAAGATTCAGAAGGTTTGTAACAAGA AATTGTGGGAAAGATATACACACCGGAGAAAAGAAGTTTCTGAAGAAAACCACAACCATGCAAACGAAAG GATGTTATTTCATGGGTCTCCTTTTGTGAATGCGATTATCCATAAGGGCTTTGATGAAAGGCATGCATAC ATAGGTGGCATGTTTGGAGCTGGAATTTATTTTGCTGAGAACTCTTCCAAAAGCAATCAATATGTGTATG GAATTGGAGGTGGCACCGGATGTCCAATTCACAAAGACAGATCGTGTTACATTTGTCACAGGCAGCTGCT GTTTTGTCGAGTAACCTTGGGAAAGTCTTTCTTGCAGTTCAGCGCAATGAAAATGGCACATTCTCCTCCC GGCCATCACTCGGTCACTGGCCGGCCCAGTGTAAATGGCCTAGCATTAGCTGAATATGTTATTTACAGAG GAGAACAGGCTTATCCTGAATATCTAATTACTTACCAGATTGTAAGGCCTGAAGGTATGGTTGATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215283 representing NM_001163635 Red=Cloning site Green=Tags(s) MSGRRCAGGGAACASAGAEAVEPSARELFEACRNGDVERVKRLVTPEKVNSRDTAGRKSTPLHFAAGFGR KDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLQHGADPNARDNWNYTPLHEAAIKGKIDVC IVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGDYKKDELLESARSGNEEKMMALLTPLNVNCHASDGR KSTPLHLAAGYNRVKIVQLLLHHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTP LHEAASKNRIEVCSLLLSYGADPTLLNCHNKSAIDLAPTAQLKERLSYEFKGHSLLQAAREADVTRIKKH LSLEMVNFKHPQTHETALHCAAASPYPKRKQICELLLRKGANTNEKTKEFLTPLHVASENAHNDVVEVVV KHEAKVNALDSLGQTSLHRAAHCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGASLGHS EADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGL VPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLD LVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQ HGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGAD PTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPTCYKPQVLSGVRGPGATADALSSGPSSPSSLSAASS LDNLSGSFSELSAVVSSSAAEGATGLQRKEDSGIDFSITQFIRNLGLEHLMDIFEREQITLDVLVEMGHK ELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNNSGSGTILIDLSPDDKEFQSVEEEMQSTVREHR DGGHAGGVFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAY IGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPIHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPP GHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIVRPEGMVDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001163635
ORF Size	3498 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001163635.1, NP_001157107.1
RefSeq Size	6503 bp
RefSeq ORF	3501 bp
Locus ID	74493
UniProt ID	Q3UES3
MW	127.2 kDa
Gene Summary	Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. Stimulates 26S proteasome activity.[UniProtKB/Swiss-Prot Function]

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