Akap6 (NM_198111) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI482140; Akapalpha; Akapbeta; PRKA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215225 representing NM_198111 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAACCATGAGTGTGACCCTTTCCCCACTGCGGTCCCAGGACCCAGACCCCATGGCGACTGATGCCT CGCCCATGGCCATCAACATGACACCTACCGTGGAGCAGGAGGAAGGAGAGGGAGAAGAGGCCGTGAAGGA CGTGGGTGCTGAGCAGCAGTATGGAAAGCCACCTCCACTCCACACAGCAGCCGACTGGAAGATTGTCCTG CACTTACCTGAGATTGAGACCTGGCTCCGGATGACCTCAGAGAGGGTCCGTGACCTAACCTACTCGGTCC AGCAGGATGCAGACAGCAAGCATGTGGATGTGCATCTAGTTCAGCTAAAGGATATTTGCGAGGATATTTC CGACCACGTGGAGCAGATCCATGCCCTCCTTGAGACGGAGTTCTCCCTGAAGCTGCTGTCCTACTCGGTC AATGTCATCGTAGACATCCACGCAGTGCAGCTGCTCTGGCACCAGCTCCGCGTATCCGTGCTGGTCCTCC GGGAGCGCATCCTACAAGGTCTGCAGGACGCCAATGGCAACTACACCAGGCAGACTGACATTCTGCAAGC ATTCTCTGAAGAAACAACAGAGGGCCGGCTTGACTCCCTCACAGAAGTGGACGACTCTGGGCAGTTAACT ATCAAATGCTCTCAGAATTATTTGTCTCTGGATTGTGGTATTACTGCCTTTGAACTTTCTGACTACAGTC CAAGTGAGGATCTGCTTGGTGGCCTGGGCGACATGACCACTAGCCAGGCCAAAACTAAATCGTTTGACTC TTGGAGTTACAGCGAGATGGAAAAGGAATTTCCCGAGCTCATCCGAAGCGTTGGGCTGCTTACAGTGGCC ACTGAGCCTGTCCCTTCCAGCTGTGGAGCAGCCAATGAGGACTCATCTCAAGTGATCCTTTCAGAGGACC ACAGAGGTGGACATGAGGAAGATGGCGCTCTTGAACCTGGAGAGCAGCTGGGCTCAACACTGGAGACGTC TTCCTTAGGGGACACACTGACAAACGCTGCTGAACACCCTCCGGAGACAGCAAACCAAGACTCTACTTCC TCTCCGCAGCTTGGTGCGAAAAAATCCCAGCCTGGTCCTTGTGAAATGATGACTCCCAAGAGATCCATCC GCGATTGCTTTAATTATAACGAGGACTCTCCCACGCAGCCCACATTGCCCAAAAGAGGGCTATTTCTAAA AGAAACTCTTAAGAACGAGCGGAGAGGCAGTGATGGGAAGGGGAGGGTGGTTGACCTGAAGCCTGAGCTG AGCAGAAGCACCCCGTCCCTGGTGGAACCCCCTGACAGATCGAAGCTCTGCCTAGTGTTGCAGTCTTCCT ACCCCAGCAGCCCTTCTGCCGCCAGCCAGTCCTATGAGTGTTTGCACAAGGTGGGGATCGGCAATCTTGA AAACATAGTCAGAAGTCACATTAAAGAAATTTCTTCCAGTTTGGGAAGGCTTACTGACTGCCATAAAGAG AAACCGCGACTGAAAAAGCCTCACAAGACCTTGGCCGAGGTGTCCCTGTGCAAAATACCTAAACGGGGAA CTGGTTCAGGAAAGCAATCCGAGAATACCAGGAGCTCAGTCGTGCCGACCATGGTGTCTCCTGGAGCTCC GAAAGCCACGGCGAGACCAGCAACAGATTCTGCGTCTACAACCTCAGGTGACACGTGCCATCAGAGAAAT CGGGGTGGAAAATTGCCAGCGCAGTCGAAGGCCTCCAGCTCATCCCCTTGTAGTCACAGCAGTGAGTCCT CTCTTGGCTCAGACAACATCAAATCCCCACTTCCTCTCCTTTCAAAAAGCCAAAAAGGCTCCCCACCCGC TCCATGTCACGCCACACAGAACGGTCAGGTGGTGGAGGCCTGGTACGGCTCTGATGAATACCTAGCGCTG CCATCTCACCTCAAACAAACGGAGGTGTTAGCTCTGAAACTGGAGAACCTAACCAAGCTTCTACCCCAGA AACCCAGAGGAGAGACTATCCAGGATATTGATGACTGGGAACTGTCTGAGATGAATTCAGACTCTGAAAT CTATCCAACGTACCATATCAAGAAAAAACACACAAGGCTAGGCACCGTCTCTCCGAGCTCATCCAGCGAC ATAGCCTCATCTCTCGGGGAGAGCATTGAATCTGGGCCCCTGAGTGACATTCTTTCTGATGAGGACTTAT GTATGCCCCTCTCCGGCATGAAAAAGTTCACTGATGAGAAATCAGAGAGACCTTCATCCTCTGAGAAGAA TGAGAGCCATTCTGCTACAAAATCAGCCTTAATTCAGAAACTAATGAAAGATATTCAGCACCAAGATAAC TATGAAGCCATCTGGGAAAGAATTGAGGGGTTTGTGAACAAGCTGGACGAATTCATTCAGTGGCTAAACG AAGCCATGGAGACCACGGAGAACTGGACTCCGCCTAAAGCCGAGACCGACAGCCTCCGGCTGTACCTGGA GACACACTTGAGCTTTAAGTTGAACGTAGACAGTCACTGTGCCCTCAAGGAAGCCGTGGAGGAAGAAGGA CACCAACTTCTTGAGCTCATTGCGTCTCACAAAGCAGGACTGAAGGACATGCTGAAGATGATTGCAAGTC AATGGAAGGAGCTGCAGAGGCAAATCAAACGGCAACACAGCTGGATTCTCAGAGCCCTGGACACCATCAA AGCCGAGATACTGGCTACTGATGTGTCTGTGGAGGACGAGGAGGGGACGGGGAGCCCCAAGGCTGAGGCT CAGCTCTGCTACCTGGAAGCTCAGAGAGACGCTGTGGAGCAGATGTCCCTCAAGCTGTACAGCGAGCAGT ATACCAGTGGTAGCAAGAGGAAAGAAGAGTTTGCCGACATGTCGAAAGCGCATTCGGTGGGAAGCAATGG GCTTCTGGACTTTGATTCAGAATATCAGGAGCTCTGGGATTGGCTGATTGACATGGAGTCCCTTGTGATG GACAGCCACGACCTGATGATGTCAGAGGAGCAGCAGCAGCATCTTTACAAGCGCTACAGCGTGGAAATGT CCATCAGGCACCTGAAAAAGACAGAGCTACTTAGCAAGGTTGAAGCTTTGAAGAAAGGTGGCCTTTCACT ACCAAATGATATCCTGGAAAAAGTGGATTCAATTAATGAAAAATGGGAGCTGCTTGGGAAAACCCTAAGA GAGAAGATACAGGACACAATGGCAGGCCACAGTGGGTCGGGCCCACGTGACCTGCTCTCTCCTGAAAGCG GAAGCCTGGTAAGGCAGCTGGAGGTCAGGATCAAAGAGCTGAAAAGGTGGCTAAGAGATACAGAGCTTTT CATCTTCAATTCCTGTCTGAGACAAGAGAAGGAAGGAACGAGCGCCGAGAAACAACTCCAATACTTTAAG TCGCTCTGTCATGAGATCAAGCAGCGGCGTAGAGGAGTGGCCTCCATTCTGAGGTTGTGCCAGCACCTTC TGGATGACCGGGACACTTGTAATCTGAACGCAGATCACCAGCCCATGCAGCTGATCATTGTAAACCTTGA AAGAAGGTGGGAAGCCATCGTCATGCAAGCTGTCCAGTGGCAAACACGGTTACGAAAGAAGATGGGAAAA GAATCTGAGACTCTGAATGTGATTGACCCTGGCTTGATGGACCTGAATGGAATGAGTGAGGATGCCCTGG AATGGGATGAGACAGACATAAGTAACAAACTCATTAGTGTGCATGAAGAATCCAACGACCTTGATCAAGA CCTGGAGCCTATGGTCCCAACAGTGAAACTTGAAGAGACACACCACAAGGACTCCGGTTATGAAGAGGAG GCAGGTGACTGTGGAGGGTCCCAGTATACCTCAAATATCACTGCCCCTTCCAGCCCACACATTTACCAAG TGTACAGTCTCCACAATGTTGAGTTCCACGAGGACAGCCACACTCAGTTTCTAAAAAGCAGCCCCAAGTT CACAGGCACAGTGCAGCCCACTGTTTTAACTAAGAGCCTCAGTAAAGATTCTTCATTTTCATCCACAAAA TCGTTACCAGACCTTTTAGGGGGTTCCAGCTTGGTGAGGCCTTACTCGTGTCAGAGTGGAGACTTGAGCC AGAATTCAGGCAGTGAGAGTGGAATTGTCAGCGAAGGAGACAATGAGATGCCAACCAACTCCGACATGAG CTTGTTCAGTATGGTCGATGGGTCCCCGAGTAACCCTGAAACACAACATCTGGACCCACAAATGGGAGAC GCAGCCAATGTGCTGGAGCAAAAATTTAAAGATGAGGGGGAGTGCATTAAGCTTTCAAGTGTCTCTCAGG CATCTGTCTCACCAGTGGGTTGTGTAAATGGGAAAGCTGGGGACTTAAACAGTATTACCAAACACACCAC TGATTGTTTGGGAGACGAATTACAAGGAAAACATGAGGTGTTTACATTTTATGACTACTCGTACCTCCAA GGCTCAAAACTCAAATTCCCAATGATAATGAAACAGCCGCAGAGTGAGAAGGCACATTTGGAGGATCCCC TTCTTCGTGGTTTCTATTTTGATAAAAAGTCCTGCAAACCTAAACATCAGACTTCTGAGTCACAACCAGA CGCGCCCCTCCATGAAAAGATCTTGGCAAGTGCCCCCCACGAGATGGGTCGAAGCTCATACAAAAGTAGC GACATAGAGAAGGCACTCACGGGCATCCAGAATGCCCGACAGCTCTCCCTTTTATCTCGTAGCTCATCTG TAGAGTCCCTTTCCCCAGGGGGTGATTTGTTTGGATTGGGAATCTTTAAAAATGGCAGTGACAGCCTCCA GCGAAGCACTTCTTTAGAAAGTTGGTTGACATCCTATAAGAGCAACGAGGATCTTTTTAGCTGTCACAGC TCTGGGGACATAAGTGTGAGCAGTGGCTCGGTTGGTGAGCTGAGCAAGAGGACATTAGATCTTTTGAATC GTCTGGAGAACATACAGAGCCCCTCAGAGCAAAAGATCAAGCGGAGTGTTTCAGATATCACTCTGCAAAG CAGTTCCCAAAAGATGTCCTTCAGTGGCCAGATGTCACTGGATGTCGCATCCTCTATCAATGAAGACTCG CCGGCATCTCTTACCGAACTGAGTAGCAGCGATGAGCTCTCTCTTTGCTCGGAGGACATTGTGTTACACA AAAACAAGGTCCCAGAGTCCAACGCATCATTCAGGAAGCGCCTGAATCGCTCGGTGGCTGATGAGAGCGA CGTCAATGTCAGCATGATTGTCAACGTCTCCTGCACCTCTGCTTGCACTGATGACGAAGACGACAGCGAC CTCCTCTCCAGCTCCACTCTCACCTTGACGGAAGAAGAGCTGTGTCTCAAAGATGAGGATGACGACTCCA GTATCGCAACGGATGATGAAATTTATGAGGAAAGCAACCTGATGTCTGGGCTGGACTACATCAAGAACGA ACTGCAGACGTGGATAAGACCCAAACTTTCCTTGTCGAGAGAAAAGAAACGGCCCAGTGTCACTGATGAA ATAAAGGTCAATAAAGATGGAGGAAGTACCGAGAGGGCCAATCCCTCGGACACCCTGGACATTGAGGCCC TTCTAAATTGCTCCATAAAACGTCTCTCTGAAAACAATGGGAATGGCAAGAACCCACCCAGAACTCATGA CTTAGGAACCAAAGGTGAAAATAAGAAAAATACGTACGACGTTACTAAAGATCCACATGTGGCCGACATG GAAAATGGCAATGTTGAAAGTACTCCAGAGAGAGAAAAGGAGAAGCCAGAGATCCCAGAGGCATCAGCGA ACCTTGCTTCAAATGTGAAAAAGATTTCTGAATCCAAGCACGACGAGTATGAAGCATTAATGGATGGTTC TGATGATTCAAGTGTCACTGGGAAGGAATTTGGTCCCCCAAATGATAGGCATCCTCCACAGATTGGTGCC GATCCCCAGCATCCTGAAAGGGGTGACTGCACATCAGTCCAGAACTCTTGCTCAGGACTACTGTTGGAAA CCGGTGGCGGAAGCAGACAAGATAGTGATGGACTCAAATCTTTGTCTAATGATGCACCAAGTGTGGCTAG AAACGCTGCTGGTTGCTGCCTACTCGAACAAAATGAGACAGAGGAAAGTGCTTCTATCAGCAGCAGCACT TCCTGTTGCAACTGCAAGCCAGATGTTTTCCATCAGAAAGATGATGAAGATTGTTCGGTACATGACTTTG TTAAGGAAATCATTGACATGGCATCAACAGCCCTCAAAAGTAAATCACAACCTGAGAGTGAGGTGGCCGC TCCCACGTCACTGACCCAAATTAAGGAGAAAGTGTTAGAGCATTCGCATCGGCCCATACACCTGAGGAAG GGAGATTTTTACTCCTACCTATCTCTTTCGTCCCATGACAGTGACTGTGGGGAGGTCACCAATTACATAG ATGAGAAGAGCAACACCCCATTGCCACCAGACACCGTGGACTCTGGCTTAGATGACAAGGAAGACGTGGA CTGCTTCTTTGAAGCTTGTGTTGAGGACGAGCCTGCTGATGAGGAAGCCCGTCTCTCCAGTGCCCTTCCA AATGAATCAGAAGTCCAGGATGAAGCTGCTAAGCCAGAACAAATGACAGCCAGCTCTTCTGTGTTCCGTG ATGAGACAGACACGGTGCCTCTTTCAGGACTTTCCCCTCAGAAGGGAGCTGATGATGCAAAGGAAGGAGA TGGTGCGTCCCACACTTCCCAGGGCTGTGTGGAGAGCGCAGAGCCTACCACCCCCCCAGGAAAGGCCAAG AGAGAGGGGAGTTCAAGAAAGCAAAGTGTATCAGGAACGCCAGAAGAAAACGCTGCTTCAGCCAAACCGA AAATTCAAGCTTTCTCTTTGAATGCAAAACAGCCAAAAGGCAAGGCTGCACTGTATCCCAGCCCCCAAAC TTTAACCTGCAAAGAGAAGCTCGTAAGCTTTCATGAAGATCGACATAGTAACATGCATAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215225 representing NM_198111 Red=Cloning site Green=Tags(s) MLTMSVTLSPLRSQDPDPMATDASPMAINMTPTVEQEEGEGEEAVKDVGAEQQYGKPPPLHTAADWKIVL HLPEIETWLRMTSERVRDLTYSVQQDADSKHVDVHLVQLKDICEDISDHVEQIHALLETEFSLKLLSYSV NVIVDIHAVQLLWHQLRVSVLVLRERILQGLQDANGNYTRQTDILQAFSEETTEGRLDSLTEVDDSGQLT IKCSQNYLSLDCGITAFELSDYSPSEDLLGGLGDMTTSQAKTKSFDSWSYSEMEKEFPELIRSVGLLTVA TEPVPSSCGAANEDSSQVILSEDHRGGHEEDGALEPGEQLGSTLETSSLGDTLTNAAEHPPETANQDSTS SPQLGAKKSQPGPCEMMTPKRSIRDCFNYNEDSPTQPTLPKRGLFLKETLKNERRGSDGKGRVVDLKPEL SRSTPSLVEPPDRSKLCLVLQSSYPSSPSAASQSYECLHKVGIGNLENIVRSHIKEISSSLGRLTDCHKE KPRLKKPHKTLAEVSLCKIPKRGTGSGKQSENTRSSVVPTMVSPGAPKATARPATDSASTTSGDTCHQRN RGGKLPAQSKASSSSPCSHSSESSLGSDNIKSPLPLLSKSQKGSPPAPCHATQNGQVVEAWYGSDEYLAL PSHLKQTEVLALKLENLTKLLPQKPRGETIQDIDDWELSEMNSDSEIYPTYHIKKKHTRLGTVSPSSSSD IASSLGESIESGPLSDILSDEDLCMPLSGMKKFTDEKSERPSSSEKNESHSATKSALIQKLMKDIQHQDN YEAIWERIEGFVNKLDEFIQWLNEAMETTENWTPPKAETDSLRLYLETHLSFKLNVDSHCALKEAVEEEG HQLLELIASHKAGLKDMLKMIASQWKELQRQIKRQHSWILRALDTIKAEILATDVSVEDEEGTGSPKAEA QLCYLEAQRDAVEQMSLKLYSEQYTSGSKRKEEFADMSKAHSVGSNGLLDFDSEYQELWDWLIDMESLVM DSHDLMMSEEQQQHLYKRYSVEMSIRHLKKTELLSKVEALKKGGLSLPNDILEKVDSINEKWELLGKTLR EKIQDTMAGHSGSGPRDLLSPESGSLVRQLEVRIKELKRWLRDTELFIFNSCLRQEKEGTSAEKQLQYFK SLCHEIKQRRRGVASILRLCQHLLDDRDTCNLNADHQPMQLIIVNLERRWEAIVMQAVQWQTRLRKKMGK ESETLNVIDPGLMDLNGMSEDALEWDETDISNKLISVHEESNDLDQDLEPMVPTVKLEETHHKDSGYEEE AGDCGGSQYTSNITAPSSPHIYQVYSLHNVEFHEDSHTQFLKSSPKFTGTVQPTVLTKSLSKDSSFSSTK SLPDLLGGSSLVRPYSCQSGDLSQNSGSESGIVSEGDNEMPTNSDMSLFSMVDGSPSNPETQHLDPQMGD AANVLEQKFKDEGECIKLSSVSQASVSPVGCVNGKAGDLNSITKHTTDCLGDELQGKHEVFTFYDYSYLQ GSKLKFPMIMKQPQSEKAHLEDPLLRGFYFDKKSCKPKHQTSESQPDAPLHEKILASAPHEMGRSSYKSS DIEKALTGIQNARQLSLLSRSSSVESLSPGGDLFGLGIFKNGSDSLQRSTSLESWLTSYKSNEDLFSCHS SGDISVSSGSVGELSKRTLDLLNRLENIQSPSEQKIKRSVSDITLQSSSQKMSFSGQMSLDVASSINEDS PASLTELSSSDELSLCSEDIVLHKNKVPESNASFRKRLNRSVADESDVNVSMIVNVSCTSACTDDEDDSD LLSSSTLTLTEEELCLKDEDDDSSIATDDEIYEESNLMSGLDYIKNELQTWIRPKLSLSREKKRPSVTDE IKVNKDGGSTERANPSDTLDIEALLNCSIKRLSENNGNGKNPPRTHDLGTKGENKKNTYDVTKDPHVADM ENGNVESTPEREKEKPEIPEASANLASNVKKISESKHDEYEALMDGSDDSSVTGKEFGPPNDRHPPQIGA DPQHPERGDCTSVQNSCSGLLLETGGGSRQDSDGLKSLSNDAPSVARNAAGCCLLEQNETEESASISSST SCCNCKPDVFHQKDDEDCSVHDFVKEIIDMASTALKSKSQPESEVAAPTSLTQIKEKVLEHSHRPIHLRK GDFYSYLSLSSHDSDCGEVTNYIDEKSNTPLPPDTVDSGLDDKEDVDCFFEACVEDEPADEEARLSSALP NESEVQDEAAKPEQMTASSSVFRDETDTVPLSGLSPQKGADDAKEGDGASHTSQGCVESAEPTTPPGKAK REGSSRKQSVSGTPEENAASAKPKIQAFSLNAKQPKGKAALYPSPQTLTCKEKLVSFHEDRHSNMHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_198111
ORF Size	6921 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_198111.2, NP_932779.2
RefSeq Size	10263 bp
RefSeq ORF	6924 bp
Locus ID	238161
MW	254.8 kDa

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