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Gls (NM_001113383) Mouse Tagged ORF Clone
CAT＃: MR215212
- TrueORF®
Gls (Myc-DDK-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
"NM_001113383" in other vectors (6)
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CNY 4,496.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6330442B14; AI314027; B230365M23Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215212 representing NM_001113383
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCGGCTGCGAGGCTCGGCGATGCTACGGGAGCTGCTCTTAAGGCCGCCCGCCGCCGTCGGAGCCG TCCTGCGGCGCGCGCAGCCCCTCGGCACGCTGTGCCGGCGCCCCCGGGGCGGGAGCCGGCCGACCGCTGG CCTGGTGGCCGCTGCGCGACTCCACCCGTGGTGGGGCGGGGGCGGCCGCGCGAAGGGCCCCGGCGCGGGC GGCCTGTCCAGTTCGCCCTCGGAGATCCTACAGGAGCTGGGGAAGGGGGGTACGCCGCCACAGCAGCAGC AGCAGCAGCAGCAGCAGCCAGGGGCGTCGCCACCCGCAGCCCCGGGCCCCAAGGACAGCCCGGGGGAGAC CGACGCGTTCGGCAACAGCGAGGGCAAGGAGATGGTGGCCGCGGGCGACAATAAAATAAAACAGGGTCTG TTACCTAGCTTGGAAGATTTGCTGTTCTATACAATTGCAGAAGGACAAGAAAAGATACCTGTTCACAAGT TTATTACAGCACTCAAATCTACAGGATTGCGAACATCTGATCCCAGGTTGAAAGAGTGTATGGATATGTT AAGATTAACTCTTCAGACAACGTCAGATGGTGTCATGCTAGACAAAGATCTTTTTAAAAAGTGTGTTCAA AGCAACATTGTTTTGTTGACACAAGCATTTAGAAGAAAGTTTGTCATTCCTGACTTTATGTCTTTTACCT CACACATCGATGAGTTATATGAAAGTGCTAAAAAGCAGTCTGGAGGGAAGGTTGCTGATTATATTCCTCA GCTGGCCAAATTCAGTCCTGATTTGTGGGGTGTATCTGTCTGTACTGTAGATGGGCAAAGGCATTCTATT GGAGATACCAAAGTTCCTTTTTGTCTTCAGTCCTGTGTAAAACCCCTGAAATATGCAATTGCTGTTAATG ACCTGGGAACTGAGTATGTACATCGCTATGTTGGGAAGGAGCCAAGTGGATTAAGATTCAACAAACTCTT TTTGAATGAAGATGATAAACCACATAATCCTATGGTAAATGCTGGAGCAATTGTTGTGACTTCTCTAATA AAGCAAGGAGTAAATAATGCTGAGAAGTTTGACTACGTGATGCAATTTTTGAATAAGATGGCTGGTAATG AATATGTTGGATTCAGTAATGCAACGTTTCAGTCTGAACGAGAAAGTGGAGATCGAAATTTTGCAATAGG ATATTACTTAAAAGAAAAGAAGTGTTTTCCAGAAGGCACAGACATGGTTGGGATACTAGATTTTTACTTC CAGCTGTGCTCTATTGAAGTGACATGTGAATCAGCAAGTGTGATGGCTGCCACCTTGGCTAATGGTGGTT TCTGCCCAATTACTGGTGAAAGAGTCCTCAGTCCTGAGGCAGTTCGGAATACACTGAGCTTGATGCATTC TTGTGGCATGTATGACTTCTCAGGGCAGTTTGCGTTCCATGTTGGTCTTCCTGCAAAATCTGGAGTTGCT GGGGGTATTCTTTTAGTTGTCCCCAACGTCATGGGCATGATGTGTTGGTCTCCTCCTCTTGACAAGATGG GCAACAGTGTTAAGGGAATTCACTTTTGTCACGATCTTGTTTCTCTGTGTAACTTCCATAACTATGATAA TTTGAGACACTTTGCAAAAAAACTTGATCCTCGGAGAGAAGGAGGTGATCAAAGGCATTCCTTTGGACCA TTGGACTATGAGAGTCTCCAGCAAGAACTTGCTTTAAAAGACACAGTATGGAAAAAAGTGTCACCTGAGT CAAGTGACGACACCTCTACAACTGTAGTATATAGAATGGAGAGTCTGGGGGAGAGGAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR215212 representing NM_001113383
Red=Cloning site Green=Tags(s) MMRLRGSAMLRELLLRPPAAVGAVLRRAQPLGTLCRRPRGGSRPTAGLVAAARLHPWWGGGGRAKGPGAG GLSSSPSEILQELGKGGTPPQQQQQQQQQPGASPPAAPGPKDSPGETDAFGNSEGKEMVAAGDNKIKQGL LPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQ SNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSI GDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLI KQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYF QLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVA GGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRHSFGP LDYESLQQELALKDTVWKKVSPESSDDTSTTVVYRMESLGERS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001113383 |
| ORF Size | 1812 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113383.1, NP_001106854.1 |
| RefSeq Size | 4419 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 14660 |
| UniProt ID | D3Z7P3 |
| MW | 66 kDa |
| Gene Summary | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219497 | Gls (untagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug) |
CNY 7,700.00 |
|
| MG215212 | Gls (tGFP-tagged) - Mouse glutaminase (Gls) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug) |
CNY 5,800.00 |
|
| MR215212L1 | Lenti ORF clone of Gls (Myc-DDK-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6,896.00 |
|
| MR215212L2 | Lenti ORF clone of Gls (mGFP-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6,896.00 |
|
| MR215212L3 | Lenti ORF clone of Gls (Myc-DDK-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7,130.00 |
|
| MR215212L4 | Lenti ORF clone of Gls (mGFP-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7,130.00 |
