Cfap20 (NM_008187) Mouse Tagged ORF Clone
CAT#: MR214189
- TrueORF®
Gtl3 (Myc-DDK-tagged) - Mouse gene trap locus 3 (Gtl3)
ORF Plasmid: tGFP
"NM_008187" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2600014O15Rik; AL024127; Gtl3; T10-2A2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR214189 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAAGAACACGTTCCAGAGTGGCTTCCTTTCCATCCTCTACAGCATCGGCAGCAAGCCCCTGCAGA TCTGGGACAAAAAGGTACGGAATGGCCACATCAAAAGAATCACTGACAATGACATCCAGTCCCTGGTGCT AGAGATTGAAGGGACAAATGTCAGTACCACATACATCACGTGTCCTGCGGACCCCAAGAAGACACTGGGG ATCAAACTGCCTTTCCTTGTCATGATCATCAAAAACTTGAAGAAATATTTTACCTTTGAAGTCCAGGTAC TAGATGACAAAAACGTTCGTCGAAGGTTTCGAGCAAGTAACTACCAGAGTACCACTCGAGTCAAGCCCTT CATCTGTACCATGCCCATGCGGCTGGATGATGGCTGGAACCAGATTCAGTTCAACTTGTCAGATTTCACC CGGCGGGCATATGGCACGAACTACATCGAGACCCTGAGAGTGCAGATCCATGCAAACTGCCGTATCCGAA GGGTTTACTTCTCAGACAGACTCTACTCAGAAGATGAGCTGCCAGCAGAGTTCAAACTCTATCTACCAGT TCAGAACAAGGCCAAACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR214189 protein sequence
Red=Cloning site Green=Tags(s) MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLG IKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFT RRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNKAKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008187 |
ORF Size | 582 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008187.2, NP_032213.2 |
RefSeq Size | 1318 bp |
RefSeq ORF | 582 bp |
Locus ID | 14894 |
UniProt ID | Q8BTU1 |
MW | 22.7 kDa |
Gene Summary | Cilium- and flagellum-specific protein that plays a role in axonemal structure organization and motility. Involved in the regulation of the size and morphology of cilia. Required for axonemal microtubules polyglutamylation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208608 | Gtl3 (untagged) - Mouse gene trap locus 3 (Gtl3), (10ug) |
CNY 3,990.00 |
|
MG214189 | Gtl3 (tGFP-tagged) - Mouse gene trap locus 3 (Gtl3), (10ug) |
CNY 4,000.00 |
|
MR214189L3 | Lenti ORF clone of Gtl3 (Myc-DDK-tagged) - Mouse gene trap locus 3 (Gtl3) |
CNY 4,750.00 |
|
MR214189L4 | Lenti ORF clone of Gtl3 (mGFP-tagged) - Mouse gene trap locus 3 (Gtl3) |
CNY 4,750.00 |