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Zbtb4 (NM_029348) Mouse Tagged ORF Clone
CAT＃: MR213663
- TrueORF®
Zbtb4 (Myc-DDK-tagged) - Mouse zinc finger and BTB domain containing 4 (Zbtb4)
ORF Plasmid: tGFP
"NM_029348" in other vectors (4)
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CNY 7,192.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310026P19Rik; 9230111I22Rik; mKIAA1538 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR213663 representing NM_029348
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACCCCCTGCAGAGGTGACGGACCCATCCCATGCCCCAGCTGTCCTGCATCAGCTCAATGAGCAGC GGCTCCGTGGCCTCTTCTGTGACGTCACCCTCATAGCTGGAGACACCAAGTTCCCTGCTCACCGCAGTGT CCTGGCCGCCTCTAGTCCCTTCTTCAGAGAAGCCCTACTAGCTTCAGCCCCACTGCCCCTTCCACCAGTT ACTGGCGGCTCAGCCCCCAGCCCCGCAACCACCACAGCTGCCTCCTCTTCGTCCTCCTCTCCCCCTCCAG CCTCTCCTCACTCTTCATCTCCTCCCCGGGTGCTAGAGCTGCCCGGGGTCCCAGCAGCTGCCTTCTCCGA TGTCCTCAACTTCATCTATAGTGCCCGGCTTGCACTCCCTGGCGGTGGTGGGGATGGGGCAGCTGTGGCA GAGATTGGAGCTCTGGGCCGGAGGCTGGGTATCTCCCGTCTACAGGGCCTGGGGGAAGGAGGTGATACTT GGGTACCACCTGCCCCAACTTCCATGGTCACCTCAGATCCTACTGAGGATGGTTTGGGGGCAGGGCCTAG AACAGATGGAGAGTGGGTGGGTGACAAAGCTGAGGCCCTGACTCCTGACTCCCAGCCCCGGCGGCCCTTT CCTTGCCCTCGATGTGGAAAAAGCTTCATCCATCCCAAGCGACTACAGACCCACGAGGCTCAGTGTCGAC GAGGGTCCAACACTCGGGGGTCTGCAGGGCTGGGGCCTGGAGTCTCTGGCTCTGGGGGTCCTGCAGGAGT GGATGCCTCTGCCCTGCCACAACCAGTGGGCTTCAGAGATGGCCCTGAGCATGTGGTGAAGGTTGTGGGC GGCCACGTGCTCTATGTGTGTGCGGCCTGTGAGCGTTCCTACGTGACCCTGTCCAGCCTGAAGCGGCACA GCAATGTACACTCGTGGCGGAGGAAGTACCCCTGCCGCTACTGTGAGAAGGTGTTTGCCCTGGCTGAGTA CCGCACCAAGCACGAGGTGTGGCACACTGGGGAGCGCAGGTACCAGTGCATCTTCTGCTGGGACACCTTT GTCACCTATTATAACCTGAAGACCCACCAGCGAGCCTTCCATGGCATTAGCCCTGGCCTCCTAGCCAGTG AGAAGACACCCAATGGAGGCTACAAACCCAGGCTTAATACCCTCAAGCTGTACCGCCTGCTCCCTATGCG GGCGGCCAAGCGGCCCTACAAGACCTACAGTCAGGGAGCCCCTGAGGCCCCTCTTTCTCCAAGCCTCCAC ACACCGGCCCCCGCGGCAATGCCAGCCAGCCCCCAACCCCTACCCCCACCCGCCCCAGAGCCTGGTCCTC CCCCCTCTGTCATCACCTTTGCTCATCCGGCTCCCTCTGTCATTGTCCACGGGAGCAGTAGTAGTGGTGC AGCCGGGGGCGGACCAGCCGGCACAGGAGGGTCCCAAGCTGCCTCAGTCATCACTTATACGACTCCCCCA AGACCCCCCAAGAAACGAGAGTACCCACCTCCTCCCCCTGAGCCTACAGCAACACCCACCAGCCCAGCCT CCACCGCCGTCAGCCCAGCCACAGCTGCAGGGCCAGCCACAGCCACAGAGGAGGCCAAGGGCCGGAATCT GCGGGCTGGGAGGACTCTGACTTACACAGCCAAACCCGTGGGTGGGTTGAGTGGGAGTGGGGGTTCCCCC ACAGGGACTGGCCGAGGCTCTTCTCAGCTTCAGGCTCCACCTCCACTGTGTCAGATCACTGTGCGAATTG GGGAAGAGGCCATTGTCAAGCGCCGCATCTCAGAAACTGACCTGCGTCCTGGAGAGCTGAGTGGAGAGGA AGTAGAGGAGAGCGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGACCAGGAGGAACAAGAGGAATCGAAG GCTGGAGGGGAAGATCAGCTTTGGAGACCCTACTATTCATACAAGCCTAAGCGCAAGGCTGGAGCTACTG CCGGCGGGGCCAGCGGGGTCAGTGGACTGCCCCGAGGACGAAGACCACCACGCTGGAGGCAAAAGCTGGA ACGAAGGGGCTGGGAGGAGACCCCATCAGTGGAGGGCCCAGGAGGACGAGGACGTGGTGAGCGTAGGCAC CGTTGTGGGGACTGTGCCCAGGCCTTTGCCACTGTGAGGAAGCTGAGAAAGCACCAGGAAGCCCATAGTG GAGGCTCCCACACCTCCAGGACTGGGAGGAGGTCTTCCACCCGCTTCACCTGTCCCCACTGTGCCAAGGT GTGCAAGACGGCAGCTGCCCTGAACCGACATGGGCAAAGGCATGCTGTGGAACGGCCCGGGGGCACTCCC ACACCTGTCATTGCCTATTCCAAAGGAAGCATTGGCACCAGGCCCACTGACGTCAAGGAGGAGGCCCCCC AGGAGATGCAAGTGTCCTCTTCCAGTGGGGAGGCAGGCAGTGGTAGCGCTGCCGCTGCTGAAGCTTCTGA GTCTGCCTCACTCCAGGACCCGGTCATCTCTGGGGGTGAGGAGCCTCCAGTAGCAGGTGGGGGCAGCTAT GTATACCCACCTGTGCAGGAATTTCCCCTGGCCCTGATAGGAGGCAGCCGGGAGCCCAGTGCCGGCAAAG GAAAACCTGGGAATGAGGGTTCCTTGGGAGCTTCTGAGGGTGACCGGATGGAGGGGATGGGGACTGCCAA AGTCACCTTCTACCCTGAGCCCTACCCACTTGTCTATGGCCCCCAGCTCCTTGCTGCCTACCCTTACAAC TTCAGCAACTTGGCTGCTCTCCCAGTTGCTCTCAACATGGTCCTACCTGACGAAAAAGGTGGTGGGGCCC TTCCCTTCCTACCAGGGGTCTTTGGCTACGCAGTGAATCCTCAAGCGGCGCCACCCACTCCCCCTCCCCC TCTCCCTCTGCCAGTTTCCCCTAAGGGAATAGGGGGAATGACAGGGGTAGAGAGAACCCAGAAGGGGGAT GTGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR213663 representing NM_029348
Red=Cloning site Green=Tags(s) MPPPAEVTDPSHAPAVLHQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPV TGGSAPSPATTTAASSSSSSPPPASPHSSSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVA EIGALGRRLGISRLQGLGEGGDTWVPPAPTSMVTSDPTEDGLGAGPRTDGEWVGDKAEALTPDSQPRRPF PCPRCGKSFIHPKRLQTHEAQCRRGSNTRGSAGLGPGVSGSGGPAGVDASALPQPVGFRDGPEHVVKVVG GHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWDTF VTYYNLKTHQRAFHGISPGLLASEKTPNGGYKPRLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLH TPAPAAMPASPQPLPPPAPEPGPPPSVITFAHPAPSVIVHGSSSSGAAGGGPAGTGGSQAASVITYTTPP RPPKKREYPPPPPEPTATPTSPASTAVSPATAAGPATATEEAKGRNLRAGRTLTYTAKPVGGLSGSGGSP TGTGRGSSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEVEESEEEEEEEEEEDQEEQEESK AGGEDQLWRPYYSYKPKRKAGATAGGASGVSGLPRGRRPPRWRQKLERRGWEETPSVEGPGGRGRGERRH RCGDCAQAFATVRKLRKHQEAHSGGSHTSRTGRRSSTRFTCPHCAKVCKTAAALNRHGQRHAVERPGGTP TPVIAYSKGSIGTRPTDVKEEAPQEMQVSSSSGEAGSGSAAAAEASESASLQDPVISGGEEPPVAGGGSY VYPPVQEFPLALIGGSREPSAGKGKPGNEGSLGASEGDRMEGMGTAKVTFYPEPYPLVYGPQLLAAYPYN FSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAPPTPPPPLPLPVSPKGIGGMTGVERTQKGD VG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029348 |
| ORF Size | 2946 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_029348.2, NP_083624.2 |
| RefSeq Size | 7824 bp |
| RefSeq ORF | 2949 bp |
| Locus ID | 75580 |
| UniProt ID | Q5F293 |
| MW | 103.1 kDa |
| Gene Summary | Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA. Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222952 | Zbtb4 (untagged) - Mouse zinc finger and BTB domain containing 4 (Zbtb4), (10ug) |
CNY 12,640.00 |
|
| MG213663 | Zbtb4 (tGFP-tagged) - Mouse zinc finger and BTB domain containing 4 (Zbtb4), (10ug) |
CNY 7,890.00 |
|
| MR213663L3 | Lenti ORF clone of Zbtb4 (Myc-DDK-tagged) - Mouse zinc finger and BTB domain containing 4 (Zbtb4) |
CNY 9,030.00 |
|
| MR213663L4 | Lenti ORF clone of Zbtb4 (mGFP-tagged) - Mouse zinc finger and BTB domain containing 4 (Zbtb4) |
CNY 9,030.00 |
