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Oas2 (NM_145227) Mouse Tagged ORF Clone
CAT＃: MR212435
- TrueORF®
Oas2 (Myc-DDK-tagged) - Mouse 2'-5' oligoadenylate synthetase 2 (Oas2)
ORF Plasmid: tGFP
"NM_145227" in other vectors (4)
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CNY 5,432.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Oasl11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212435 representing NM_145227
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACTGGCTGACTGGAAACTGGTCATCTGACAGGTCATCTGGCTATTCATCTGGCTGGTCACCTG GTGGGTCTTCAGGGGTGCCCTCCGGGCCAGTGCACAAGTTAGAAAAGTCTATCCAGGCAAACCTCACACC CAACGAAAACTGTCTGAAGCAGATTGCGGTGTCCTCGGTGCCATCGCAGAAGCTAGAAGGGTATATCCAG GAAAACCTCAAACCTAACAGAGAATCTCTGAAGCAGATAGACCAGGCCGTGGATGCCATCTGGGACCTGC TGCGCAGTCAGATCCCTGTGAAGGAAGTGGCTAAGGGTGGCTCCTATGGCCGGGAAACAGCCCTAAGAGG CTGCTCCGATGGTACCCTTGTTCTCTTCATGGACTGCTTCCAACAGTTCCAGGATCAGATAAAATACCAA GATGCATACCTTGACGTCATTGAACTGTGGCTGAAAATCCATGAGAAGAAGTCAGTAAAGCATGAACATG CCCTTGTAGTACAAGTGTCTGTACCAGGGCAGAGAATACTCCTGCAATTACTTCCAGTCTTCAATCCTCT ACGCTCCAATGAGAATCCCAGCTCCTGTGTCTATGTGGATCTCAAAAAATCCATGGATCAAGTAAGAGCC TCACCAGGGGAGTTCTCAGACTGCTTCACCACACTGCAGCAGCGGTTTTTCAAGAAATATCCCCAAAGAC TGAAGGATTTGATCCTATTGGTCAAGCACTGGTATGAACAGTGCCAGGAGAAGTGGAAAACACCCCCACC TCAGCCATTGCTGTACGCACTGGAACTGCTCACTGTGTATGCCTGGGAACAGGGCTGCCAAGCTGAAGAC TTCGACATGGCACAAGGCGTCAGGACCGTGCTGCGACTTATCCAGCGGCCGACAGAGCTGTGTGTCTACT GGACAGTCAATTACAACTTTGAGGATGAGACAGTCCGGAACATCCTTCTGCACCAGCTCAGGTCCCAAAG ACCAGTCATCTTGGATCCAACTGACCCAACCAATAATGTGGGCAAAGATGATGGGTTCTGGGAGCTACTG ACAGAGGAAGCTATGGCCTGGCTGTACTCTCCCAGCCTGAATACTGAGTCACCTGCACCATATTGGGATG TTCTGCCCATGCCACTTTTCGTCACTCCAAGCCACTTACTGAACAAGTTCATCAAGGACTTTCTCCAGCC CAACAAGCTCTTCCTAAAGCAGATCAAGGAAGCTGTTGACATTATATGTTCCTTCCTTAAAAATGTCTGC TTCTTGAATTCTGACACCAAAGTCCTGAAGACCGTCAAGGGAGGATCCACTGCCAAAGGCACAGCTCTGA AGCGGGGATCAGATGCTGACATTGTTGTGTTCCTCTCCTCGCTGGAGAGTTACGACTCTCTAAAAACCAA CCGCTCCCAGTTCGTCCAGGAGATCCAGAAGCAGTTAGAAGAATTCGTGCAGGCGCAGGAGTGGGAGGTG ACGTTTGAGATTTCAAAATGGAAGGCTCCCAGAGTGCTGAGTTTTACCTTGAAATCCAAGACTCTCAATG AAAGTGTCGAGTTCGATGTCCTTCCCGCCTATGATGCACTAGGTCAACTGCGGTCTGACTTCACCCTCAG GCCCGAAGCCTACAAGGATCTCATTGAGCTGTGTGCATCACAGGACATCAAAGAAGGAGAGTTTTCTATC TGTTTTACTGAGCTGCAGAGAAACTTCATTCAAACCCGGCCCACCAAACTGAAGAGTCTACTCCGCCTGA TCAAGCACTGGTACAAACAGTATGAAAGGAAGATGAAGCCAAAAGCATCTTTACCCCCAAAGTACGCCCT GGAGCTGCTCACCGTGTATGCCTGGGAGCAGGGCAGTGGCACAGATGACTTTGACATTGCTGAAGGCTTC CGGACCGTCCTGGACCTGGTTATAAAATACCGGCAGCTCTGCATCTTCTGGACAGTCAATTACAACTTTG AAGAGGAATACATGCGGAAGTTCCTACTGACCCAGATCCAGAAAAAGAGGCCTGTAATCCTGGATCCAGC AGATCCCACAGGCGATGTGGGAGGAGGTGACCGCTGGTGCTGGCATCTTCTAGCTGAAGAAGCGAAGGAG TGGCTGTCCTCCCCTTGTTTCCAAGTGGAGCAAAAAGGCCTGGTACAGCCTTGGAAAGTGCCAGTAATGC AGACCCCCGGAAGCTGTGGAGGTCAGATCTACCCCACTGTGGGTGGAGTTACTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212435 representing NM_145227
Red=Cloning site Green=Tags(s) MGNWLTGNWSSDRSSGYSSGWSPGGSSGVPSGPVHKLEKSIQANLTPNENCLKQIAVSSVPSQKLEGYIQ ENLKPNRESLKQIDQAVDAIWDLLRSQIPVKEVAKGGSYGRETALRGCSDGTLVLFMDCFQQFQDQIKYQ DAYLDVIELWLKIHEKKSVKHEHALVVQVSVPGQRILLQLLPVFNPLRSNENPSSCVYVDLKKSMDQVRA SPGEFSDCFTTLQQRFFKKYPQRLKDLILLVKHWYEQCQEKWKTPPPQPLLYALELLTVYAWEQGCQAED FDMAQGVRTVLRLIQRPTELCVYWTVNYNFEDETVRNILLHQLRSQRPVILDPTDPTNNVGKDDGFWELL TEEAMAWLYSPSLNTESPAPYWDVLPMPLFVTPSHLLNKFIKDFLQPNKLFLKQIKEAVDIICSFLKNVC FLNSDTKVLKTVKGGSTAKGTALKRGSDADIVVFLSSLESYDSLKTNRSQFVQEIQKQLEEFVQAQEWEV TFEISKWKAPRVLSFTLKSKTLNESVEFDVLPAYDALGQLRSDFTLRPEAYKDLIELCASQDIKEGEFSI CFTELQRNFIQTRPTKLKSLLRLIKHWYKQYERKMKPKASLPPKYALELLTVYAWEQGSGTDDFDIAEGF RTVLDLVIKYRQLCIFWTVNYNFEEEYMRKFLLTQIQKKRPVILDPADPTGDVGGGDRWCWHLLAEEAKE WLSSPCFQVEQKGLVQPWKVPVMQTPGSCGGQIYPTVGGVTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145227 |
| ORF Size | 2226 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145227.3, NP_660262.2 |
| RefSeq Size | 3873 bp |
| RefSeq ORF | 2229 bp |
| Locus ID | 246728 |
| UniProt ID | E9Q9A9 |
| MW | 85.5 kDa |
| Gene Summary | Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response (PubMed:12396720, PubMed:29117179). Activated by detection of double stranded RNA (dsRNA): polymerizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNASEL) leading to its dimerization and subsequent activation (PubMed:29117179). Activation of RNASEL leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication (PubMed:21142819). Can mediate the antiviral effect via the classical RNASEL-dependent pathway or an alternative antiviral pathway independent of RNASEL (PubMed:21142819). In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation (PubMed:21142819). May act as a negative regulator of lactation, stopping lactation in virally infected mammary gland lobules, thereby preventing transmission of viruses to neonates (PubMed:29117179). Non-infected lobules would not be affected, allowing efficient pup feeding during infection (PubMed:29117179).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221262 | Oas2 (untagged) - Mouse 2'-5' oligoadenylate synthetase 2 (Oas2), (10ug) |
CNY 5,984.00 |
|
| MG212435 | Oas2 (tGFP-tagged) - Mouse 2'-5' oligoadenylate synthetase 2 (Oas2), (10ug) |
CNY 6,370.00 |
|
| MR212435L3 | Lenti ORF clone of Oas2 (Myc-DDK-tagged) - Mouse 2'-5' oligoadenylate synthetase 2 (Oas2) |
CNY 7,700.00 |
|
| MR212435L4 | Lenti ORF clone of Oas2 (mGFP-tagged) - Mouse 2'-5' oligoadenylate synthetase 2 (Oas2) |
CNY 7,700.00 |
