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Yap1 (NM_009534) Mouse Tagged ORF Clone
CAT＃: MR212130
- TrueORF®
Yap1 (Myc-DDK-tagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2
"NM_009534" in other vectors (3)
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CNY 3,656.00
CNY 4,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI325207; Y; Yap; Yap65; Yk; Yki; yor; Yorkie |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212130 representing NM_009534
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCGCAACAGCCGCCGCCCCAGCCGGCCCCGCAAGGCCCCGCGCCGCCGTCCGTGTCTCCGG CCGGGACCCCCGCGGCCCCGCCCGCACCCCCGGCCGGCCACCAGGTCGTGCACGTCCGCGGGGACTCGGA GACCGACTTGGAGGCGCTCTTCAATGCCGTCATGAACCCCAAGACGGCCAACGTGCCTCAGACCGTGCCC ATGCGGCTTCGCAAGCTGCCCGACTCCTTCTTCAAGCCGCCTGAGCCCAAGTCCCACTCGCGACAGGCCA GTACTGATGCAGGTACTGCGGGAGCTCTGACTCCACAGCATGTTCGAGCTCACTCCTCTCCAGCCTCCCT GCAGCTGGGTGCCGTTTCTCCTGGGACACTCACAGCCAGTGGCGTTGTCTCTGGCCCTGCCGCTGCCCCT GCAGCTCAGCATCTCCGGCAGTCCTCCTTTGAGATCCCTGATGATGTACCACTGCCAGCAGGCTGGGAGA TGGCCAAGACATCTTCTGGTCAAAGATACTTCTTAAATCACAACGATCAGACAACAACATGGCAGGACCC CCGGAAGGCCATGCTTTCGCAACTGAACGTTCCTGCGCCTGCCAGCCCAGCGGTGCCCCAGACGCTGATG AATTCTGCCTCAGGACCTCTTCCTGATGGATGGGAGCAAGCCATGACTCAGGATGGAGAAGTTTACTACA TAAACCATAAGAACAAGACCACATCCTGGCTGGACCCAAGGCTGGACCCTCGTTTTGCCATGAACCAGAG GATCACTCAGAGTGCTCCAGTGAAGCAGCCCCCACCCTTGGCTCCCCAGAGCCCACAGGGAGGCGTCCTG GGTGGAGGCAGTTCCAACCAGCAGCAGCAAATACAGCTGCAGCAGTTACAGATGGAGAAGGAGAGACTGC GGTTGAAACAACAGGAATTATTTCGGCAGGAATTAGCTCTGCGCAGCCAGTTGCCTACACTGGAGCAGGA TGGAGGGACTCCGAATGCAGTGTCTTCTCCTGGGATGTCTCAGGAATTGAGAACAATGACAACCAATAGT TCCGATCCCTTTCTTAACAGTGGCACCTATCACTCTCGAGATGAGAGCACAGACAGCGGCCTCAGCATGA GCAGCTACAGCATCCCTCGGACCCCAGACGACTTCCTCAACAGTGTGGATGAAATGGATACAGGAGACAC CATCAGCCAAAGCACCCTGCCGTCACAGCAGAGCCGCTTCCCCGACTACCTGGAAGCCCTCCCTGGGACA AATGTGGACCTTGGCACACTGGAAGGAGATGCAATGAACATAGAAGGGGAGGAGCTGATGCCCAGTCTGC AGGAAGCGCTGAGTTCCGAAATCTTGGACGTGGAGTCTGTGTTGGCTGCCACCAAGCTAGATAAAGAAAG CTTTCTCACGTGGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212130 representing NM_009534
Red=Cloning site Green=Tags(s) MEPAQQPPPQPAPQGPAPPSVSPAGTPAAPPAPPAGHQVVHVRGDSETDLEALFNAVMNPKTANVPQTVP MRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTASGVVSGPAAAP AAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHNDQTTTWQDPRKAMLSQLNVPAPASPAVPQTLM NSASGPLPDGWEQAMTQDGEVYYINHKNKTTSWLDPRLDPRFAMNQRITQSAPVKQPPPLAPQSPQGGVL GGGSSNQQQQIQLQQLQMEKERLRLKQQELFRQELALRSQLPTLEQDGGTPNAVSSPGMSQELRTMTTNS SDPFLNSGTYHSRDESTDSGLSMSSYSIPRTPDDFLNSVDEMDTGDTISQSTLPSQQSRFPDYLEALPGT NVDLGTLEGDAMNIEGEELMPSLQEALSSEILDVESVLAATKLDKESFLTWL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009534 |
| ORF Size | 1416 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009534.3 |
| RefSeq Size | 4115 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 22601 |
| UniProt ID | P46938 |
| MW | 51.2 kDa |
| Gene Summary | This gene encodes a protein which binds to the SH3 domain of the Yes proto-oncogene product, a tyrosine kinase. This protein contains a WW domain, consisting of four conserved aromatic amino acids including two tryptophan residues. This conserved WW domain is found in various structural, regulatory and signaling molecules in various species, and may play a role in protein-protein interaction. Following cellular damage, phosphorylation of this encoded protein may suppress apoptosis. This protein may be involved in malignant transformation in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216427 | Yap1 (untagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2, (10ug) |
CNY 3,656.00 |
|
| MR212130L3 | Lenti ORF clone of Yap1 (Myc-DDK-tagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2 |
CNY 6,650.00 |
|
| MR212130L4 | Lenti ORF clone of Yap1 (mGFP-tagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 2 |
CNY 6,650.00 |
