Sptbn2 (NM_021287) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA0302; Spnb3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212122 representing NM_021287 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCACTCTGTCACCCACTGACTTTGACAGCTTGGAGATCCAGGGCCAGTACAGTGACATCAACA ACCGCTGGGACCTGCCTGACTCAGACTGGGACAATGACAGCAGTTCGGCCCGCCTCTTTGAGAGGTCCAG GATCAAGGCCCTGGCAGATGAGCGAGAAGCCGTGCAGAAGAAAACCTTCACCAAGTGGGTGAACTCCCAC CTGGCCCGGGTGACATGCCGGGTGGGAGACCTGTACAGCGACCTGCGGGACGGGCGCAACCTCCTGAGGC TCCTGGAGGTGCTCTCGGGAGAGACCCTGCCAAAACCTACCAAGGGCCGAATGCGGATTCACTGCCTGGA GAATGTAGATAAAGCGCTGCAGTTCCTGAAGGAGCAGAAGGTGCACTTGGAAAACATGGGCTCCCATGAC ATTGTGGACGGAAACCACCGTCTGACCCTTGGGCTAGTGTGGACCATCATCCTCCGATTTCAGATCCAAG ACATCAGTGTGGAGACAGAAGACAACAAGGAGAAGAAGTCAGCCAAGGATGCCCTGCTGCTGTGGTGCCA GATGAAGACTGCAGGATACCCCAATGTCAATGTGCACAACTTTACCACCAGTTGGAGAGATGGGCTGGCC TTTAATGCCATTGTGCACAAACACCGGCCAGACCTGTTGGATTTTGAGTCCCTGAAGAAGTGTAATGCAC ACTACAATCTGCAGAATGCTTTCAATCTGGCTGAAAAGGAACTTGGCCTGACGAAGCTCCTGGATCCTGA AGATGTGAATGTAGACCAGCCCGACGAGAAGTCTATCATCACCTACGTGGCCACTTACTACCACTACTTC TCCAAGATGAAGGCCCTGGCTGTGGAAGGCAAAAGAATTGGCAAGGTCCTAGACCATGCCATGGAGGCAG AACACCTGGTAGAGAAATACGAGTCCCTGGCCTCTGAACTGCTGCAGTGGATTGAGCAAACGATCGTGAC CCTCAATGACCGACAGCTGGCCAACTCCCTGAGTGGAGTCCAGAACCAGCTGCAGTCTTTCAACTCCTAT CGCACTGTGGAGAAGCCACCCAAGTTCACGGAGAAAGGGAACTTGGAGGTGTTGCTCTTCACCATCCAGA GCAAGCTACGGGCCAACAACCAGAAGGTCTACACACCCCGGGAAGGCCGGCTCATCTCGGACATCAACAA GGCCTGGGAGCGGCTGGAGAAAGCCGAACATGAGCGAGAGCTAGCGCTGCGCACAGAGCTGATCCGCCAG GAGAAGCTGGAGCAACTGGCTGCTCGCTTCGACCGCAAGGCTGCCATGCGGGAGACCTGGCTCAGTGAGA ACCAGCGACTTGTGTCCCAGGACAACTTTGGGCTGGAACTGGCAGCAGTGGAGGCTGCAGTGAGAAAGCA TGAAGCCATTGAGACAGACATTGTCGCCTACAGTGGCCGGGTGCAGGCGGTGGATGCTGTAGCTGCAGAA CTGGCTGCTGAGCACTACCATGACATTAAGCGCATTGCGGCACGTCAGAACAACGTGGCCCGGCTCTGGG ACTTCTTACGGCAAATGGTGGCTGCCCGCCGTGAGAGACTTCTTCTCAACCTGGAGCTGCAGAAAGTGTT TCAGGACCTGCTCTACCTCATGGACTGGATGGCAGAGATGAAGGGCCGACTGCAGTCTCAGGACTTGGGC AAGCACCTGGCTGGAGTGGAGGACCTCCTGCAGCTACACGAACTGGTGGAGGCGGACATCGCGGTTCAGG CTGAGAGGGTTCGAGCGGTCAGCGCCTCTGCCCTGCGTTTCTGCGACCCAGGGAAAGAGTATAGGCCATG TGACCCACAGCTGGTGTCAGAGAGGGTGGCCACTCTGGAGCAGAGCTATGAGGCCCTGTGTGAATTGGCA GCGACTAGAAGGGCCCGACTGGAAGAGTCACGTCGTCTCTGGAGGTTCCTCTGGGAAGTGGGTGAGGCTG AGGCCTGGGTCCGGGAGCAGCAGCACCTCCTGGCCTCAGCTGATACAGGCCGGGACCTGACTGGTGTCCT TCGCCTGCTCAACAAGCACGCAGCCCTACGGGGTGAGATGAGTGGCCGTCTGGGGCCCCTGAAGCTCACC CTGGAGCAAGGTCAGCAGTTAGTCGCTGAGGGCCACCCTGGAGCCAACCAAGCCTCAACCCGTGCAGCCG AGCTCCAGGCCCAGTGGGAGCGACTAGAAGCCCTGGCCGAGGAGCGAGCCCAGCAGCTGGCACAGGCTGC CAGCCTCTACCAGTTCCAGGCAGATGCAAATGACATGGAGGCCTGGTTGGTGGATGCACTGCGCCTGGTA TCTAGCCCCGAGGTAGGGCACGACGAGTTCTCCACACAGGCCCTGGCCAGGCAGCACAGGGCCCTTGAAG AGGAGATCCGAGCCCACCGGCCTACACTGGATGCCCTGAGGGAGCAGGCTGCAGCCCTGCCACCTGCACT GAGCCACACACCGGAGGTACAGGGCAGGGTGCCCACTCTGGAGCAGCACTATGAGGAGCTGCAGGCCCGA GCCGGTGAGCGTGCACGAGCCCTGGAGGCAGCCCTGGCGTTCTATACCATGCTCAGCGAGGCTGGGGCCT GTGGCCTCTGGGTAGAGGAGAAGGAGCAATGGCTCAACGGGCTGGCCCTACCTGAGCGCCTGGAGGACCT GGAGGTGGTCCAGCAGAGGTTTGAGACCCTGGAGCCTGAAATGAACGCCCTGGCTGCACGGGTTACTGCT GTCAATGACATCGCTGAGCAGCTGCTGAAGGCCAGTCCACCAGGCAAGGACCGCATCATTGGCACCCAGG AGCAGCTCAACCAAAGGTGGCAGCAGTTTCGGTCCCTGGCAGATGGCAAAAAGGCGGCTCTGACATCAGC CCTGAGCATCCAGAATTACCACCTAGAGTGCACAGAGACCCAGGCCTGGATGAGAGAGAAGACTAAGGTC ATTGAGTCCACCCAGGGCCTGGGCAATGATCTAGCTGGTGTGCTGGCCCTGCAGCGGAAGTTGGCGGGTA CTGAGAGAGATCTGGAAGCTATCTCTGCCCGGGTGGGTGAGCTGACCCAAGAGGCAAATGCCTTGGCTGC TGGGCACCCAGCTCAAGCTCCTGCCATCAACACCCGGCTTGGAGAGGTACAAGCAGGATGGGAAGATCTT CGGGCGACCATGAGGCGGAGAGAAGAGTCCCTGGGTGAGGCTCGGCGGCTGCAGGATTTCCTGCGCAGCT TGGATGACTTCCAGGCCTGGCTAGGCCGCACACAGACCGCTGTAGCCTCCGAAGAAGGGCCAGCCACCCT TCCCGAGGCAGAAGCCCTCTTAGCCCAGCACGCAGCTCTGCGGGGAGAAGTGGAGAGAGCCCAGAGCGAG TACAGCCGCCTCAGGACCTTGGGCGAGGAGGTGACCAGAGACCAGGCTGATCCCCAGTGCCTCTTCCTCA GACAGAGGCTGGAAGCCCTTGGAACCGGCTGGGAGGAGCTGGGTCGCATGTGGGAGAGCCGGCAAGGCCG CCTGGCCCAGGCCCATGGCTTCCAGGGGTTTTTAAGAGATGCTCGCCAGGCTGAGGGTGTTCTCAGCAGC CAGGAATATGTCTTGTCTCACACGGAGATGCCAGGGACACTGCAGGCGGCAGATGCAGCCATTAAAAAGC TGGAAGACTTCATGAGCACTATGGACGCCAATGGAGAGCGCATCCGCGGACTCCTGGAGGCTGGACGTCA GCTGGTGTCCAAGGGCAATATCCATGCTGAGAAGATCCAAGAGAAGGCGGACTCCATCGAGAAGAGGCAC AGAAAGAATCAGGAGGCGGTGCAGCAGCTTTTAGGACGCCTTCGGGACAACCGAGAGCAGCAGCACTTCT TGCAAGACTGTCAGGAGCTGAGACTCTGGATCGATGAGAAGATGCTGACAGCTCAGGATGTGTCCTATGA CGAAGCTCGCAACCTGCACACCAAGTGGCAGAAACACCAGGCATTCATGGCCGAGCTTGCAGCCAACAAG GACTGGCTGGACAAAGTGGACAAGGAAGGGCGGGAGCTGACTCTTGAAAAGCCAGAACTCAAAGTCGTAG TGTCAGAGAAGCTGGAGGACCTGCACCGGCGCTGGGATGAACTGGAGACCACTACCCAAGCCAAGGCCCG CAGTCTTTTTGATGCCAACCGGGCAGAGCTATTTGCTCAAAGCTGTTCTGCCCTGGAGAGCTGGCTGGAG AGCCTGCAGGCCCAGCTGCACTCAGATGACTATGGCAAAGACCTCACCAGTGTCAACATTCTGCTGAAGA AGCAACAGATGCTGGAACGAGAGATGGCTGTGAGAGAGAAGGAGGTAGAGGCGATACAGGCCCAGGCCCA GGCCCTGGCCCAGGAAGACCAGAGTGCAGGGGAGGTAGAAAGGACCTCAAGGGCTGTGGAGGAGAAGTTC AGGGCCTTGTGTCAACCCATGAAAGAACGCTGCCGGCGCCTGCACGCCTCCCGGGAGCAGCACCAGTTCC ACCGGGATGTGGAGGATGAGATATTGTGGGTGACTGAGCGGCTTCCCATGGCCAGCTCTCTGGAACATGG CAAGGACTTGCCCAGTGTCCAGCTTCTCATGAAGAAAAACCAGACCCTGCAGAAGGAGATCCAGGGCCAT GAGCCCCGGATTGCAGACCTCAAAGAGAGGCAGCGCACTCTGGGAACAGCAGCAGCAGGTCCAGAGCTGG CTGAGCTCCAAGAAATGTGGAAACGCCTGAGCCATGAGCTGGAGCTTCGGGGCAAGCGGCTGGAGGAAGC TCTTCGAGCCCAGCAATTCTATCGTGATGCTGCAGAGGCCGAGGCTTGGATGGGTGAGCAGGAGTTACAC ATGATGGGCCAGGAAAAGGCCAAGGATGAGCTGAGCGCCCAGGCAGAAGTGAAGAAGCATCAGGTATTGG AACAAGCCCTTGCTGACTATGCCCAGACCATCAAACAACTAGCAGCCAGCAGTCAAGATATGATTGACCA TGAACATCCAGAGAGCACAAGACTAACAATACGCCAAGCCCAGGTGGACAAGCTGTACGCCAGCCTGAAG GAGCTGGCAGGAGAGCGGCGCGAGCGCCTGCAGGAGCACCTCCGGCTGTGCCAGCTCCGCAGAGAGCTGG ATGACCTGGAACAGTGGATACAGGAGCGTGAAGTTGTGGCAGCCTCCCATGAACTGGGCCAAGACTATGA GCACGTGACTATGCTTCGGGACAAATTCCGAGAGTTCTCCAAGGACACCAGCACCATTGGCCAGGAGCGC GTAGACAGTGCCAATGCACTGGCCAATGGGCTCATTGCTGGGGGCCATGCTGCACGGGCCACTGTGGCCG AGTGGAAGGACAGTCTCAATGAGGCCTGGGCTGACCTGCTGGAGCTGCTGGACACAAGAGGTCAGGTGCT GGCTGCTGCGTATGAGCTGCAGCGCTTCCTGCATGGAGCCCGCCAAGCCCTGGCACGGGTACAGCACAAG CAGCAGCAGCTTCCAGACGGGACTGGCCGTGACCTCAATGCTGCTGAGGCCCTGCAGCGCCGGCACTGTG CCTATGAGCACGACATCCAAGCCCTCAGCACTCAGGTCCAGCAAGTTCAAGATGATGGCCAAAGGCTACA GAAGGCCTATGCTGGAGACAAGGCTGAGGAAATTGGCCGTCACATGCAGGCAGTGGCTGAGGCCTGGGCC CAGCTCCAGGGAAGTTCTGCTGCCCGCCGACAGCTGTTACTGGATACCACGGACAAATTCCGATTCTTTA AGGCTGTCCGAGAGTTGATGCTGTGGATGGATGGGATTAACCTGCAGATGGATGCCCAGGAGCGGCCCCG GGATGTGTCCTCTGCAGATTTAGTCATCAAAAACCAACAAGGAATCAAAGCAGAGATAGAGGCCAGAGCA GACAGGTTCTCCTCCTGCATTGACATGGGGCAGGAGCTGCTGGCCCGGAGCCACTATGCTGCTGAGGAGA TCTCAGAGAAGCTGTCTCAGCTACAGTCCCGGCGCCAGGAGACAGCTGACAAGTGGCAGGAGAAGATGGA CTGGCTACAGCTTGTTTTGGAGGTGCTTGTGTTTGGGAGAGATGCAGGGATGGCGGAGGCCTGGCTGTGC AGTCAGGAGCCATTGGTGCGAAGTGCAGAACTGGGTTGCACTGTGGACGAAGTAGAGAGCCTCATCAAGC GACACGAAGCCTTTCAGAAGTCGGCCGTGGCCTGGGAAGAGCGTTTCAGTGCGCTGGAGAAGCTCACTGC GCTGGAAGAGCGGGAAAATGAGCGGAAAAGGAAGAGGGAGGAGGAGGAACGGAGGAAACAGCCTCCTACT TCAGAGCCCATGGCTAGTCAGCCAGAAGGGAGTCTGGTAGATGGCCAGAGAGTTCCTGACACTGCCTGGG ATGGGACTCAGTCAAAATTGCCACCATCCACACAAGCACCCAGTGTTAATGGGGTCTGCACGGACACCGA CTCCTCACAGCCCCTGTTGGAACAGCAGAGACTTGAACAGAGCAATGTCCCCGAAGGACCTGGATCTGGC ACAGGAGACGAGTCCAGTGGGCCCCGGGGAGAGAGGCAGACCCTGCCCCGGGGCCCTGCTCCTTCTCCAA TGCCCCAGAGCAGATCATCTGAAGCAGCTCATGGTGCCACCCTGCCCACACGAGGACCTGAGCTCTCTGC TCAGGAACAGATGGAAGGGATGCTGTGCCGCAAACAAGAGATGGAAGCCTTCAACAAGAAAGCTGCCAAC AGGTCATGGCAGAATGTGTACTGTGTACTTCGGCGTGGGAGCCTCGGCTTTTACAAGGATGCCAGGGCAG CTAGTGCAGGAGTGCCCTACCATGGAGAAGTGCCTGTCAGCCTGGCCAGGGCCCAGGGAAGTGTGGCCTT TGATTATCGGAAACGCAAACATGTTTTCAAGCTGGGCTTACAGGATGGCAAAGAATACCTATTCCAGGCC AAGGACGAGGCAGAGATGAGCTCATGGCTGAGAGTGGTGAATGCAGCCATTGCCACTGCATCCTCTGCCC CTGGAGAGTCAGAAGAGCCAGTGGTGCCCAGTGCCAGCCGGGGTCTGACCCGGGCCATGACCATGCCCCC AGTGTCACAGCCTGAGGGTTCCATCGTGCTTCGAAGCAAGGATGGCAGAGAAAGAGAGCGAGAAAAACGA TTCAGCTTCTTTAAGAAGAACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212122 representing NM_021287 Red=Cloning site Green=Tags(s) MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSH LARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHD IVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLA FNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYF SKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSY RTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQ EKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAE LAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLG KHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELA ATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLT LEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLV SSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQAR AGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTA VNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKV IESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDL RATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSE YSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSS QEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRH RKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANK DWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLE SLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKF RALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGH EPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELH MMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLK ELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQER VDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHK QQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWA QLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARA DRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLC SQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPT SEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSG TGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAAN RSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQA KDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKR FSFFKKNK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021287
ORF Size	7164 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021287.2
RefSeq Size	8254 bp
RefSeq ORF	7167 bp
Locus ID	20743
MW	270.9 kDa

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