Snrnp200 (NM_177214) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A330064G03Rik; Ascc3l1; BC011390; HELIC2; U5-200-KD; U5-200KD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212115 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATGTGACTGCCCGCAGTTTGCAGTACGAGTACAAGGCGAACTCAAATCTTGTGCTCCAAGCTG ACCGCTCTCTCATTGACCGGACCCGCCGGGATGAACCGACGGGAGAGGTTCTGTCCCTGGTTGGGAAGTT GGAGGGAACCCGAATGGGAGATAAGGCTCAACGGACCAAACCACAGATGCAGGAAGAAAGACGGGCCAAG AGGAGAAAACGTGATGAGGACCGGCATGACATGAACAAGATGAAAGGCTACACGCTGCTGTCTGAGGGCA TTGATGAGATGGTGGGCATCATCTACAAGCCTAAGACGAAGGAGACCCGAGAGACCTACGAGGTGCTGCT CAGCTTCATCCAGGCTGCGCTGGGAGATCAGCCTCGAGATATTCTTTGTGGAGCAGCTGATGAAGTCCTG GCTGTGCTGAAGAATGAAAAGCTTCGAGACAAGGAGAGAAGACGGGAGATTGACCTGCTGCTGGGTCAGA CAGATGATACCAGATACCATGTGCTGGTAAACCTGGGCAAGAAGATCACAGACTACGGTGGCGACAAGGA GATACAGAATATGGATGACAACATTGATGAGACTTATGGTGTGAACGTGCAGTTTGAGTCTGATGAGGAG GAAGGTGATGAAGATGTGTATGGGGAAGTCCGAGAAGAAGCTTCTGATGATGACATGGAAGGGGACGAAG CTGTTGTGCGCTGCACCCTCTCTGCTAATCTTGTAGCCTCAGGCGAACTGATGAGTTCTAAGAAGAAGGA TTTGCACCCTCGGGATATCGATGCCTTCTGGCTGCAGCGACAGCTCAGTCGTTTCTATGATGATGCCATT GTGTCACAGAAGAAGGCAGATGAGGTGTTGGAGATTTTGAAGACAGCAAGTGATGACCGAGAATGTGAGA ACCAACTGGTTTTGCTCCTTGGTTTCAACACGTTTGACTTCATTAAAGTGTTGCGGCAGCACAGGATGAT GATTTTATACTGTACCTTGTTGGCCAGTGCTCAGAGTGAACCTGAAAAGGAGAGGATCGTGGGAAAGATG GAAGCTGATCCAGAGCTCTCCAAGTTCCTCTATCAGCTCCATGAGACTGAGAAGGAGGACCTGATCAGAG AAGAAAGGTCCCGGAGAGAGAGAGTCCGTCAGTCCCGAATGGACACAGATCTGGAAACCATGGATTTGGA CCAGGGTGGAGAGGCTCTGGCTCCAAGGCAGGTTCTGGATTTGGAGGACCTTGTTTTTACCCAAGGAAGC CACTTCATGGCCAATAAGCGCTGCCAGCTTCCTGACGGGTCCTTCCGACGCCAGCGTAAAGGCTATGAAG AGGTGCATGTGCCTGCTTTGAAGCCCAAGCCCTTTGGCTCAGAAGAACAATTGCTCCCGGTGGAGAAGCT GCCAAAGTATGCCCAGGCAGGATTTGAGGGCTTCAAAACGCTGAACCGGATCCAGAGTAAGCTCTACCGT GCTGCCCTGGAGACTGATGAGAACCTGCTGCTGTGTGCTCCCACTGGTGCGGGCAAGACCAACGTGGCCC TGATGTGTATGCTCCGAGAGATAGGGAAACACATCAACATGGACGGCACAATCAATGTGGATGACTTCAA GATCATCTACATAGCTCCCATGCGGTCCCTGGTCCAGGAGATGGTGGGCAGTTTTGGAAAGCGCCTGGCC ACATATGGCATCACTGTTGCTGAGCTGACTGGGGATCACCAGCTATGCAAGGAGGAAATCAGTGCCACAC AGATTATCGTCTGCACCCCTGAGAAGTGGGACATCATCACACGCAAGGGCGGGGAGCGCACCTACACCCA GCTGGTGCGACTCATTGTCTTGGATGAGATCCATCTTCTACATGATGACAGAGGTCCTGTCTTAGAAGCT TTGGTGGCCAGGGCCATCCGAAACATTGAGATGACCCAAGAAGATGTCCGACTCATTGGTCTCAGTGCTA CCCTCCCCAACTATGAAGATGTGGCCACCTTTCTGCGAGTCGACCCTGCTAAGGGCCTCTTCTACTTTGA TAACAGCTTCCGCCCCGTGCCTCTGGAACAAACATATGTGGGCATCACAGAGAAAAAAGCTATCAAACGT TTCCAGATCATGAATGAAATAGTCTATGAGAAAATCATGGAACATGCTGGAAAAAATCAGGTGCTCGTGT TTGTCCATTCTCGAAAAGAAACTGGGAAGACAGCAAGGGCAATCCGTGACATGTGTCTGGAGAAGGACAC TTTGGGTCTGTTTCTTCGCGAGGGTTCTGCCTCCACTGAAGTCCTTCGTACAGAAGCAGAGCAGTGCAAG AACTTGGAGCTGAAGGATCTTTTGCCCTATGGCTTTGCTATTCATCATGCAGGCATGACTAGAGTTGACC GAACACTTGTAGAAGATCTTTTTGCTGACAAGCATATTCAGGTTTTAGTTTCCACCGCAACTCTGGCGTG GGGTGTAAATCTTCCTGCACATACAGTCATCATTAAAGGTACCCAAGTGTACAGTCCAGAGAAGGGGCGT TGGACAGAGCTGGGAGCACTGGATATCCTGCAGATGCTGGGCCGTGCTGGACGGCCGCAGTATGACACCA AGGGTGAAGGCATCCTCATCACATCCCATGGGGAGCTCCAGTACTACCTCTCCCTCCTCAACCAGCAGCT GCCTATCGAGAGCCAGATGGTCTCCAAGCTGCCTGACATGCTCAATGCGGAAATTGTTCTGGGCAATGTC CAGAATGCAAAGGATGCAGTGAACTGGCTGGGCTATGCCTACCTATACATCCGAATGCTCCGGTCCCCTA CCCTCTATGGCATTTCTCATGATGACCTCAAGGGAGATCCCTTGCTGGACCAGCGCCGACTCGATCTTGT TCACACTGCTGCCTTGATGCTGGACAAGAACAATCTGGTCAAGTACGACAAGAAGACAGGCAACTTCCAG GTGACAGAACTTGGCCGGATAGCAAGTCACTACTATATCACCAATGATACTGTGCAGACCTACAACCAGC TGCTGAAGCCTACTCTGAGTGAGATTGAGCTTTTCCGAGTGTTCTCCTTGTCCTCAGAGTTCAAGAACAT CACTGTAAGAGAGGAGGAGAAGCTGGAGCTGCAGAAGTTGCTGGAGAGAGTGCCCATCCCTGTAAAGGAG AGCATTGAGGAACCCAGCGCTAAGATCAACGTGCTTCTCCAAGCCTTCATCTCACAGCTGAAACTCGAAG GCTTTGCGCTGATGGCTGACATGGTGTATGTGACCCAGTCGGCTGGCCGGTTGATGCGTGCAATCTTCGA AATTGTCCTGAACCGAGGTTGGGCACAGCTTACAGATAAGACCCTGAATCTCTGCAAGATGATTGACAAG CGCATGTGGCAGTCCATGTGTCCTCTTCGCCAGTTCCGAAAACTTCCTGAGGAAGTAGTGAAGAAGATTG AGAAGAAAAACTTCCCCTTTGAGCGGCTGTATGACTTGAATCATAATGAGATAGGTGAACTTATTCGAAT GCCGAAGATGGGGAAGACCATCCACAAGTATGTCCATCTTTTCCCCAAGTTGGAGTTGTCAGTGCACCTG CAGCCTATTACACGCTCTACGCTGAAAGTAGAGCTGACTATCACACCAGATTTCCAGTGGGATGAAAAGG TCCATGGTTCGTCAGAGGCATTTTGGATTCTGGTGGAGGATGTGGACAGTGAGGTGATTCTGCACCATGA ATATTTTCTGCTGAAGGCCAAGTATGCCCAGGATGAGCACCTCATCACGTTCTTTGTTCCAGTCTTTGAA CCACTACCTCCTCAGTACTTCATTCGAGTAGTGTCTGATCGCTGGCTCTCTTGTGAGACGCAGCTACCTG TCTCCTTCCGGCATCTGATCCTACCAGAGAAGTACCCACCTCCAACCGAACTGTTGGACCTGCAGCCATT GCCTGTGTCTGCTCTGAGAAACAGTGCTTTTGAGAGCCTTTACCAAGATAAATTTCCTTTCTTCAATCCC ATCCAGACTCAAGTATTTAATACCGTGTACAACAGTGATGATAACGTGTTTGTGGGGGCCCCCACGGGCA GCGGGAAGACTATCTGCGCGGAGTTTGCCATCCTTCGGATGCTGCTGCAGAATTCTGAGGGACGCTGTGT CTACATTACCCCTATGGAGGCTCTGGCAGAGCAGGTATACATGGACTGGTATGAGAAGTTTCAGGACAGG CTCAACAAGAAGGTAGTGCTGCTGACGGGGGAGACCAGCACAGATCTGAAGCTCCTGGGCAAAGGCAACA TCATCATCAGTACCCCTGAGAAGTGGGACATCCTCTCTCGGAGGTGGAAGCAGCGCAAGAACGTCCAGAA CATTAACCTCTTTGTGGTGGATGAGGTCCACCTTATTGGGGGCGAGAATGGGCCTGTCTTGGAAGTGATC TGCTCCCGGATGCGCTACATCTCCTCCCAGATTGAGCGGCCCATTCGCATTGTGGCACTTAGCTCCTCAC TCTCCAATGCCAAGGATGTGGCTCACTGGCTGGGCTGCAGTGCCACCTCCACCTTCAACTTCCATCCTAA TGTGCGCCCTGTACCTTTGGAACTGCACATCCAGGGCTTCAACATCAGTCACACACAGACTCGCCTGCTC TCTATGGCCAAGCCTGTGTACCATGCTATCACCAAACACTCACCCAAGAAGCCTGTCATCGTTTTTGTCC CATCTCGTAAGCAGACCCGCCTCACTGCAATAGACATCCTCACTACCTGTGCAGCAGACATCCAGCGGCA GAGGTTCCTGCACTGCACCGAGAAGGACCTGATCCCTTACCTGGAGAAGCTCAGTGACAGCACACTCAAA GAGACCCTGTTAAATGGGGTGGGCTACCTGCATGAAGGCCTGAGCCCCATGGAGAGGCGCCTGGTAGAGC AGCTCTTCAGCTCCGGGGCTATCCAGGTGGTGGTAGCTTCTCGGAGTCTCTGCTGGGGCATGAATGTTGC TGCTCATCTAGTGATCATCATGGATACTCAGTACTACAATGGCAAGATCCATGCCTATGTGGATTACCCC ATCTATGATGTGCTTCAGATGGTGGGCCATGCCAACCGGCCCCTGCAGGATGATGAGGGGCGCTGTGTCA TCATGTGTCAGGGCTCTAAAAAGGATTTTTTCAAAAAATTTTTGTATGAGCCATTGCCAGTAGAGTCTCA CCTGGACCACTGTATGCATGACCACTTCAATGCTGAGATTGTCACCAAGACCATTGAGAACAAGCAGGAT GCTGTGGACTACCTCACCTGGACCTTTCTGTATCGCAGAATGACACAGAACCCCAATTACTACAACCTGC AGGGGATATCCCATCGTCATCTGTCTGACCACCTGTCAGAGCTGGTGGAGCAGACCCTCAGTGACCTGGA GCAGTCCAAATGCATCAGTATTGAGGACGAGATGGATGTGGCCCCTCTGAACCTGGGCATGATTGCTGCC TACTATTACATAAACTACACCACCATTGAGCTCTTCAGCATGTCTCTGAATGCTAAAACCAAGGTTCGAG GACTTATTGAGATCATTTCCAATGCAGCAGAGTATGAGAACATTCCAATCAGGCATCATGAAGACAACCT CCTGCGCCAGTTGGCTCAGAAGGTCCCCCACAAGCTGAATAACCCCAAGTTCAATGATCCACATGTGAAG ACCAATCTGCTGCTGCAGGCTCACCTGTCCCGCATGCAGCTAAGTGCTGAACTACAGTCAGACACAGAGG AGATCCTTAGTAAGGCAATCCGGCTAATTCAGGCCTGTGTGGATGTACTCTCCAGCAATGGGTGGCTTAG TCCTGCTCTGGCAGCCATGGAACTGGCCCAGATGGTCACCCAAGCCATGTGGTCTAAGGACTCTTACCTG AAGCAGCTGCCACACTTCACCTCAGAGCATATCAAACGTTGCACAGATAAGGGAGTGGAGAGTGTTTTTG ACATCATGGAGATGGAGGATGAAGAACGGAATGCATTGCTTCAGTTGACTGACAGCCAGATTGCAGATGT GGCCCGCTTCTGTAACCGCTACCCGAATATTGAACTGTCCTATGAAGTGGTGGATAAAGACAGCATCCGC AGTGGCGGACCAGTTGTGGTGCTAGTGCAACTGGAGCGAGAGGAGGAAGTCACGGGCCCAGTTATTGCAC CTCTCTTCCCACAGAAACGTGAAGAGGGCTGGTGGGTTGTGATTGGAGACGCCAAGTCCAACAGCCTCAT CTCCATCAAGAGGCTGACCCTGCAGCAGAAAGCCAAGGTGAAGCTAGACTTTGTGGCCCCAGCCACAGGT GGCCACAACTATACCCTGTACTTCATGAGTGACGCATACATGGGCTGTGACCAGGAGTATAAGTTCAGTG TGGATGTGAAAGAAGCTGAGACAGACAGTGATTCAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212115 protein sequence Red=Cloning site Green=Tags(s) MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAK RRKRDEDRHDMNKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVL AVLKNEKLRDKERRREIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEE EGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAI VSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEPEKERIVGKM EADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYR AALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLA TYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIVLDEIHLLHDDRGPVLEA LVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKR FQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCK NLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNV QNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQ VTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKE SIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDK RMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHL QPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNP IQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQNSEGRCVYITPMEALAEQVYMDWYEKFQDR LNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVI CSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLL SMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLK ETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQD AVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAA YYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVK TNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYL KQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIR SGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG GHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_177214
ORF Size	6408 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_177214.5
RefSeq Size	6760 bp
RefSeq ORF	6411 bp
Locus ID	320632
UniProt ID	Q6P4T2
MW	244.5 kDa
Gene Summary	On February 19, 2002, this locus was switched from human to mouse. The source accession, Z70200.1, is almost identical to the mouse BAC clone AC074224, and it matches the mouse cDNA accession BC011390 as well. The human gene is LocusID 23020. [provided by RefSeq, Jul 2008]

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