Nup210 (BC052468) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9830001L10, gp190, gp210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212089 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGGCCTCACTAGTGCAGCCGGCGCTCTGGGCGCTGCTGTTGCTGCAGGTAGTGGGTCCCGCCG CGGCCGCCAAGCTTAACATTCCCAAGGTGCTGCTGCCCTTCACGCGGGCCACGCGCGTGAACTTCACTCT GGAAGCTTCTGAGGGCTGCTACCGCTGGTCATCCACCCGGCCAGAGGTAGCCAGCATAGAGCCGTTGGGC TCCAGTGAACAGCAGTGCTCCCAGAAGGCAGTGGTGCAGGCCCGCTTGACCCAGCCAGCCCGCCTGACCA GCATCATCTTTGCAGAAGACATCACTACAGGGCAGGTGCTGCGCTGTGACGCCATCGTTGATCTCATCCA CGGCATTCAGATTGTCTCTACCACCCGTGAGCTCTATCTTGAGGACTCTCCACTGGAGCTGAAGATCCAG GCTCTGGACTCAGAAGGGAATACCTTCAGCACCTTGGCAGGACTGGTCTTCGACTGGACAATTGTGAAGG ATACAGAGGCCAATGGATTCTCAGACTCCCACAATGCACTCCGCATCCTCACCTTCTTGGAATCTACGTA TATCCCACCTTCCTACATCTCAGAGATGGAGAAGGCAGCCAAGCAGGGCGACACCATCCTAGTGTCTGGG ATGAAGACTGGAAGCTCCAAGCTCAAGGCTCGAATTCAGGAGGCTGTCTATAAGAATGTTCGCCCTGCGG AAGTCAGGCTGCTGATCTTGGAGAACATCCTTCTGAATCCAGCCTATGATGTCTACCTGCTGGTGGGAAC CTCCATTCACTACAAGGTTCAGAAGATCAGGCAGGGCAAGATTACAGAACTCTCAATGCCTTCTGATCAA TATGAGCTGCAGCTCCAGAACAGCATCCCAGATCCTCAGGGAGACCCCGCCCGTCCTGTGGCCATCCTGA CACAGGACACATCAAGAGTCACTGCCATGCAGATGGGACAGAGCAACCTGGTCCTGGGCCACAGGAGTAT CCGTATGCAAGGAGCTTCTAGGCTCCCCAACAGCACCATCTATGTGGTTGAAGCCGGATACCTAGGTTTC ACAGTCTACCCTGGTGACAGATGGGTGCTTGAGACTGGACATCTGTATGCAATTACCATCGAAGTATTTG ATAGGTCCAGCAACAAGGTCTACCCATCTGACAACATCCGAATTGAAGCTGTGCTTCCTGCTGAGTTTTT TGAGGTTCTGTCGTCTTCCCAGAATGGGTCTTATCATCACATCAGGGCTATCCAGAGTGGCCAGACGGCC ATCAGTGCAACCCTCACCTCTGTGGTGGACCAGGACGGAGGGGTCCATGTGCTTCAGGTACCTGTGTGGA ACCAGCAAGAAGTGGACATTCATATCCCCATCACACTCTATCCCAGCATCCTGACATTTCCATGGCAACC AAAGACAGGCGCCTATCAGTATACCATAAAGGCCCATGGGGGCAGTGGGAACTTCAGCTGGTCTTCGTCA AGCTCCATGGTTGCCACAGTCACCGTCAAGGGGGTCATGACCACAAGTGGTGACACTGGCCTCAGTGTGA TCCGGGCGCACGATGTTCAGAATCCTCTCCATTTTGGCGAGATGAAGGTGTATGTGATTGAGCCCAGCAG CATGGAGTTTGCCCCGTGCCAGGTGGAGGCTCGAGTGGGTCACACCCTGGAGCTGCCCCTGACAATCAGT GGCTTCATGCCCGGTGGGGGCAGTGAGGTGGTCACCCTGAGCGACTGCTCCCATTTCGACCTGGTGGTGG AGGTGGAGAACCAGGGTGTGTTCCAGCCATTGCCAGGGAGGCTGCCGCCAGGACCTGAGCACTGCAGTGG GGTCAAAGTGAAAGCTGATGCCCAGGGATCTACCACCCTCCTCGTGAGCTACACACACGGCCATGTACAC TTGGATGCAAAGATCACTCTTGCTGCCTACCTGCCCCTCAAGGCTGTGGATCCCTCTTCTGTTGCTGTGG TGACCCTAGGCTCCTCAAAGGAAATGCTGTTTGAAGGAGGCCCCAGGCCATGGGTCCTGGAACCTTCCAA ATTCTTTCGGAATGTCACTTCTGAAGACACAGGCAGCATCAGCCTGTCTCTCTTGGGTCCTCCAGCCTCC CGGAATTACCAGCAGCACCGGGTCCTCATGACCTGCCAGGCCTTGGGTGAGCAGCTGGACCTATCCTGCC CATTGCTGCAGCAGAATAAGCAGGTGGTTCCAGTATCCAGTCACCGTAATCCCCTGTTAGACCTGGGTGC CTATGACCAACAGGGCCGGCGATTCGATAACTTCAGCTCTCTGAGCATCCAGTGGGAGTCCTCCCGGCCA TTGCTGGCCAGCATTGAGCTTGACCAGCCCATGCAGCTGGTATCCCAGGATGATGGGAATGGCCAAAAGA AACTACATGGTTTGCAGACAGTCTCGGTTCACGAGGCATCAGGAACCACAGCCATCTCTGCCACAGCGAC TGGCTACCAGCAGTCCCACCTGAGCGAAGCCAGAGTGAAGCAACCGCATGACCCTCTAGTACCTGTGTCA GCCTCCATTGAACTTATTCTCGTAGAGGATGTGAGGGTCAGTCCAGAAGAAATGACCATTTATAACCATC CTGGTGTCCAGGTGGAACTCTACATCACAGAAGGCTCTGGATACTTCTTCCTCAACACAAGCACCCAGGA CATCATCAAGGTGGCCTACCAGGACACCCGGGGCGTAGCTCTGGTGCACCCTCTGCTCCCAGGCTCATCA ACTGTCATGGTCCATGACTTGTGCCTGGCCTTCCCAGCCCCCGCAAAGGCCATCATTCACGTCTCCGACA TCCAGGAGCTCTATGTTCGAGTGGTGGACAAGGTGGAGATCGGGAAGGCTGTCAAAGCCTACGTCCGTGT GTTGGACTTCTACAAGAAGCCTTTCCTGGCCAAGTACTTCACCTTTATGGACCTGAAGCTGCAAGCAGCC TCCCAGATAATCACGCTGGTGACTCTGGATGAAGCTCTAGACAACTACACGGCCACATTCCTCGTGCATG GTGTGGCCATTGGCCAGACCAGCCTGTCTGCAAGCGTGACGGACAAATCTGGGCAGAGAGTCAGCTCCAC CCCACAACAAATCGAGGTCTTCCCCCCATTCAGATTGATACCCAGGAAGGTGACACTGATCATTGGAGCC ATGATGCAGATCACCTCTGAGGGGGGCCCCCAGCCTCAGTCCAACATCCTCTTCTCCATCAACAATGAGA GTGTGGCAGCAGTGAGCAGCTCTGGGCTGGTGCGTGGGCTCATGGTCGGCAATGGCTCTGTGCTGGGAGT CGTGCAGGCGGTGGATGCAGAGACTGGCAAGGTCATCATCGTCTCCCAGGATCTTGTCGAAGTAGAGGTG CTACAGCTCCAGGCAGTGAGGATCCGTGCCCCAATCACACGGATGAGGACCGGGACCCAGATGCCTGTCT TTGTCACCGGAATCACCAGCAACCAGAGCCCTTTCTCCTTTGGCAATGCTGTGCCAGGCCTGACCTTCCA CTGGTCTGTCACCAAAAGGGATGTCCTGGACCTTCGAGGGCGGCACCATGAGGTATCTATCCGGCTCCCG CCTCAGTATAATTTTGCCATGAATGTGTATGGCCGAGTGAAAGGTCGGACTGGACTTCGGGTTGTAGTCA AAGCTCTGGACCCCACAGCTGGCCAGCTGCATGGTCTCGGCAAGGAGCTCTCAGATGAGATCCAAATTCA GGTATTTGAAAAACTGCGGCTGCTGAACCCTGAAATAGAAGCAGAACAAATACTAATGTCACCCAACTCA TTTATAAAGCTGCAGACAAACAGGGATGGTGCAGCCATTCTGAGCTACCGTGTCCTTGATGGGCCTGAGA AGGCTCCCATTGTTCACACTGATGAGAAGGGGTTTCTGGTGTCGGGCTCTGGAATTGGAGTGTCCACCCT CGAAGTGATTGCACAGGAGCCTTTCGGCACCAACCAGACTATCCTTGTCGCTGTAAAGGTCTCTCCTGTT TCATACCTGAGGATCTCCATGAGCCCAGTCCTGCACACTCAGCACAAGGAGGCGCTGACAGCTCTGCCTC TGGGGATGACCGTGACCTTCATTGTCCACTTCCACGACAGCTCTGGAGACATCTTCCATGCTCATAATTC AGTCCTCAATTTTGCCACTAACAGAGATGACTTCGTGCAGATCGGCAAGGGTGCCACCAACAACACCTGC ATCATCCGCACAGTCAGTGTGGGCTTGACGCTGCTCCATGTGTGGGATGTGGAGCACCTCGGCCTCTCCG ACTTCGTACCACTACCTGTCCTACAGGCCATCACCCCAGAGTTGTCTGGAGCCGTGGTGGTGGGTGACAT CCTCTGTCTGGCTAGTGTTCTTACTAGCCTGGGAGGTGTCTCAGGGACATGGAGCTCCTCAGCCAGCCAT GTCCTCTACGTTGACCCTAAGACAGGCGTGGCTATAGCCCGAGATGCAGGGTCTGTGACTGTTTACTACG AGATCGCTGGGCAGCTGAAGACCTTCAAGGAGATAGTGGTCGGCACCCCTCAGAAGATTGTGGCCCGTCG CCTCCACTCTGCCCAGACCAGTATCCAGGAAGCCACAGCCTCCAAAGTGACTGTCAGCGTGGGAGACAGG AGCTCTAACCTACTTGGTGAGTGCTCCCCTGCCCAGCGAGAGGCCATTGAAGCCTTGCACCCCGAGTCCC TCATCAGCTGCCAACTCCAGTTCAAGCAAGATGTCTTTGACTTCCCTGCTTGTGATGTCTTCACCGTGGA ACCAGGATTTGATGCTGCTCTGGGCCAGTACCTCTGCTCGGTTACTATGCGCAGGCTGACAGATAAGCAA CTGAAGCACTTGAACATGAAGAAGACATCACTGGCAGTCACTGCCTCCATCCCCAGCAGTTATACCTCTG TAGAGAAGGTGGGAGCTGAGGTCCCCTTCAGTCCAGGGCTCTATGCCAACCAGGCAGAAATCCTTTTGAG CAACCACTATACCAGCTCCGAGGTCAAAGTCTTTGGTGCTGTGGAGAGTCTGGAAAACTTGGAGGTGAAA TCTGGATCCCCGGCAGTGCTAGCCTTCGTGAAAGAGAAGTCATTTGGGCTACCCAGCTTCATCACGTACA CAGTGGGTGTCTTGGATCCCACAGCTGGCAGCCAGGGCCCTCTGTCCACTGCCCTGACCTTCTCCAGCCC TGCCACTAACCAGGCCATCACCATTCCAGTCACCGTGGCCTTTGTGTTGGATCGTCGTGGGCCTGGTCCC TATGGAGCCAGCCTCCTGTCACACTTCCTGGACTCCTATCAAGTCATGTTCTTCACCTTTTTTGCTCTGC TGGCTGGGACAGCAGTCACCATTATAGCCTACCATACAGTCTGTGCACCCCGGGAACTTGCTTCACCCCT GGCCCTCACGCCCCGTGCCAGCCCTCAGCACAGCCCCCACTATCTTGCCTCATCTCCGGCTGCTTTCAAC ACACTGCCTTCTGGTCGCAAAGCCAGCCCTCCCTCGGGGCTCTGGAGCCCAGCCTATGCCTCTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212089 protein sequence Red=Cloning site Green=Tags(s) MARASLVQPALWALLLLQVVGPAAAAKLNIPKVLLPFTRATRVNFTLEASEGCYRWSSTRPEVASIEPLG SSEQQCSQKAVVQARLTQPARLTSIIFAEDITTGQVLRCDAIVDLIHGIQIVSTTRELYLEDSPLELKIQ ALDSEGNTFSTLAGLVFDWTIVKDTEANGFSDSHNALRILTFLESTYIPPSYISEMEKAAKQGDTILVSG MKTGSSKLKARIQEAVYKNVRPAEVRLLILENILLNPAYDVYLLVGTSIHYKVQKIRQGKITELSMPSDQ YELQLQNSIPDPQGDPARPVAILTQDTSRVTAMQMGQSNLVLGHRSIRMQGASRLPNSTIYVVEAGYLGF TVYPGDRWVLETGHLYAITIEVFDRSSNKVYPSDNIRIEAVLPAEFFEVLSSSQNGSYHHIRAIQSGQTA ISATLTSVVDQDGGVHVLQVPVWNQQEVDIHIPITLYPSILTFPWQPKTGAYQYTIKAHGGSGNFSWSSS SSMVATVTVKGVMTTSGDTGLSVIRAHDVQNPLHFGEMKVYVIEPSSMEFAPCQVEARVGHTLELPLTIS GFMPGGGSEVVTLSDCSHFDLVVEVENQGVFQPLPGRLPPGPEHCSGVKVKADAQGSTTLLVSYTHGHVH LDAKITLAAYLPLKAVDPSSVAVVTLGSSKEMLFEGGPRPWVLEPSKFFRNVTSEDTGSISLSLLGPPAS RNYQQHRVLMTCQALGEQLDLSCPLLQQNKQVVPVSSHRNPLLDLGAYDQQGRRFDNFSSLSIQWESSRP LLASIELDQPMQLVSQDDGNGQKKLHGLQTVSVHEASGTTAISATATGYQQSHLSEARVKQPHDPLVPVS ASIELILVEDVRVSPEEMTIYNHPGVQVELYITEGSGYFFLNTSTQDIIKVAYQDTRGVALVHPLLPGSS TVMVHDLCLAFPAPAKAIIHVSDIQELYVRVVDKVEIGKAVKAYVRVLDFYKKPFLAKYFTFMDLKLQAA SQIITLVTLDEALDNYTATFLVHGVAIGQTSLSASVTDKSGQRVSSTPQQIEVFPPFRLIPRKVTLIIGA MMQITSEGGPQPQSNILFSINNESVAAVSSSGLVRGLMVGNGSVLGVVQAVDAETGKVIIVSQDLVEVEV LQLQAVRIRAPITRMRTGTQMPVFVTGITSNQSPFSFGNAVPGLTFHWSVTKRDVLDLRGRHHEVSIRLP PQYNFAMNVYGRVKGRTGLRVVVKALDPTAGQLHGLGKELSDEIQIQVFEKLRLLNPEIEAEQILMSPNS FIKLQTNRDGAAILSYRVLDGPEKAPIVHTDEKGFLVSGSGIGVSTLEVIAQEPFGTNQTILVAVKVSPV SYLRISMSPVLHTQHKEALTALPLGMTVTFIVHFHDSSGDIFHAHNSVLNFATNRDDFVQIGKGATNNTC IIRTVSVGLTLLHVWDVEHLGLSDFVPLPVLQAITPELSGAVVVGDILCLASVLTSLGGVSGTWSSSASH VLYVDPKTGVAIARDAGSVTVYYEIAGQLKTFKEIVVGTPQKIVARRLHSAQTSIQEATASKVTVSVGDR SSNLLGECSPAQREAIEALHPESLISCQLQFKQDVFDFPACDVFTVEPGFDAALGQYLCSVTMRRLTDKQ LKHLNMKKTSLAVTASIPSSYTSVEKVGAEVPFSPGLYANQAEILLSNHYTSSEVKVFGAVESLENLEVK SGSPAVLAFVKEKSFGLPSFITYTVGVLDPTAGSQGPLSTALTFSSPATNQAITIPVTVAFVLDRRGPGP YGASLLSHFLDSYQVMFFTFFALLAGTAVTIIAYHTVCAPRELASPLALTPRASPQHSPHYLASSPAAFN TLPSGRKASPPSGLWSPAYASH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052468
ORF Size	5526 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC052468, AAH52468
RefSeq Size	6914 bp
RefSeq ORF	5528 bp
Locus ID	54563
MW	199.5 kDa
Gene Summary	Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.[UniProtKB/Swiss-Prot Function]

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