Lamb2 (NM_008483) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW211941; Lamb-2; Lams; npht
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212084 representing NM_008483 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGGCCTCAGGAGAACCAGGGAGGGGCAGGCAGGGACAGCCTTTGCCATGGGAACTTCGCTTGG GCCTACTTCTAAGTGTGCTGGCTGCCACATTGGCCCAGGCCCCGTCCTTGGATGTACCTGGCTGTTCTCG AGGAAGCTGCTATCCAGCCACCGGTGACCTGTTGGTGGGCCGTGCGGACAGACTGACGGCCTCATCCACG TGTGGCTTGCATAGCCCTCAACCCTACTGTATTGTCAGTCACCTGCAGGACGAAAAGAAGTGTTTCCTGT GTGACTCCCGACGTCCCTTCTCTGCTCGAGACAACCCAAATAGTCATCGGATCCAGAATGTAGTCACCAG CTTTGCGCCACAACGCCGGACGGCCTGGTGGCAATCGGAGAACGGGGTTCCAATGGTCACCATCCAACTG GACCTGGAAGCTGAGTTTCATTTCACCCACCTCATTATGACGTTCAAGACGTTCCGGCCTGCTGCTATGC TGGTGGAGCGTTCTGCAGACTTTGGCCGCACCTGGCACGTGTACCGATATTTTTCCTATGACTGCGGGGC TGACTTCCCGGGAATCCCACTGGCCCCGCCACGTCGCTGGGATGATGTAGTGTGTGAGTCCCGCTACTCA GAAATCGAGCCGTCTACGGAAGGCGAGGTCATCTATCGTGTGCTGGACCCTGCTATTCCTATCCCAGACC CCTACAGCTCACGGATTCAGAACCTGTTGAAGATCACCAACCTACGAGTGAACTTAACCCGGCTTCACAC ACTGGGAGACAACTTGCTTGACCCACGGAGGGAGATCCGGGAAAAATACTATTATGCTCTCTATGAACTT GTCATCCGTGGCAACTGCTTCTGCTATGGCCACGCCTCACAGTGTGCGCCTGCACCAGGGGCGCCGGCCC ATGCTGAGGGCATGGTACACGGAGCCTGTATCTGCAAGCACAATACTCGTGGACTCAACTGTGAGCAGTG TCAGGATTTCTATCAGGACCTTCCCTGGCACCCTGCAGAGGACGGCCATACTCACGCCTGTCGGAAGTGT GAGTGCAACGGGCATACTCATAGCTGCCACTTTGACATGGCTGTCTACCTGGCATCTGGAAATGTAAGTG GAGGCGTATGCGATGGGTGTCAGCACAACACAGCTGGGCGCCATTGTGAGTTCTGCCGGCCCTTCTTCTA CCGTGACCCCACCAAGGACATGCGGGACCCAGCTGTGTGCCGTCCTTGTGACTGTGACCCTATGGGTTCT CAAGATGGTGGTCGCTGTGATTCTCATGATGACCCTGTGCTAGGACTGGTCTCAGGCCAGTGTCGCTGCA AAGAACACGTGGTTGGCACTCGCTGCCAGCAATGCCGTGATGGCTTCTTTGGACTTAGTGCCAGTGACCC TCGAGGGTGCCAGCGTTGCCAGTGTAATTCACGGGGCACAGTGCCTGGGAGCTCCCCTTGTGACTCCAGT AGTGGAACCTGTTTCTGCAAGCGTCTGGTGACCGGACATGGCTGTGACCGCTGTCTGCCTGGCCACTGGG GCCTGAGCCATGACCTGCTGGGCTGCCGTCCCTGTGACTGTGATGTGGGCGGTGCCTTGGATCCTCAGTG TGATGAGGCCACCGGTCAGTGCCGCTGCCGCCAACACATGATTGGGCGGCGCTGCGAACAAGTGCAGCCT GGCTACTTCCGGCCTTTTCTGGACCATTTAACCTGGGAGGCTGAGGCTGCCCAAGGGCAGGGGCTTGAGG TGGTAGAGCGGCTGGTGACCAACCGAGAGACTCCGTCCTGGACTGGCCCAGGCTTTGTGCGGCTGCGAGA AGGTCAGGAAGTGGAGTTCCTGGTGACCTCTTTGCCTAGGGCCATGGACTATGACCTGCTACTGCGCTGG GAGCCCCAGGTCCCTGAGCAATGGGCAGAGCTGGAACTGATGGTGCAGCGTCCGGGGCCTGTGTCTGCTC ACAGTCCGTGCGGGCATGTGCTGCCTAAGGATGACCGCATTCAGGGGATGCTTCACCCAAACACCAGGTT TTTGGTGTTTCCCAGACCTGTCTGCCTTGAGCCTGGCATCTCCTACAAGCTGAAGCTGAAACTGATCGGA ACAGGGGGACGAGCCCAGCCTGAAACCTCCTACTCTGGATTACTCATTGACTCGCTGGTCCTGCAGCCCC ACGTCTTGGTGCTAGAGATGTTTAGTGGGGGCGATGCTGCTGCTCTGGAGCGCCGTACCACCTTTGAACG CTACCGCTGCCATGAGGAAGGTCTGATGCCCAGCAAGGCCCCTCTATCTGAGACCTGTGCCCCCCTCCTC ATCAGCGTGTCCGCCTTGATCTACAATGGCGCCTTGCCATGTCAGTGTGACCCTCAAGGCTCACTGAGTT CTGAATGCAGTCCTCACGGTGGCCAGTGCCGGTGCAAACCTGGAGTGGTTGGACGCCGTTGTGATGTCTG TGCTACTGGCTACTATGGCTTTGGCCCTGCAGGCTGTCAAGCCTGCCAGTGTAGTCCTGATGGAGCACTC AGTGCCCTCTGTGAAGGGACTAGTGGACAGTGCCCCTGCCGACCTGGTGCCTTTGGTCTTCGCTGTGACC ACTGTCAACGTGGCCAGTGGGGATTCCCTAATTGCCGGCCGTGTGTCTGCAATGGGCGTGCGGATGAGTG TGATACCCACACAGGCGCTTGCCTGGGCTGCCGTGATTACACGGGGGGCGAGCACTGTGAAAGGTGCATT GCTGGTTTTCATGGGGACCCACGGCTGCCATATGGGGGCCAGTGCCGGCCTTGTCCCTGCCCTGAAGGCC CTGGGAGCCAGCGACACTTTGCTACTTCTTGCCACCGGGATGGATATTCCCAGCAAATTGTGTGCCAGTG TCGAGAAGGCTACACAGGGCTTCGGTGTGAAGCTTGTGCCCCCGGGCACTTTGGGGACCCATCAAAGCCA GGTGGCAGGTGCCAACTGTGTGAGTGCAGTGGAAACATTGATCCCATGGACCCTGATGCCTGTGATCCCC ACACGGGGCAATGCTTGCGTTGTTTACACAACACAGAGGGGCCCCACTGTGGCTATTGCAAGCCTGGCTT CCATGGGCAAGCTGCCCGACAGAGCTGTCACCGCTGTACCTGCAACCTTCTGGGCACAGATCCCAGGCGG TGCCCATCTACCGACCTGTGCCATTGTGACCCAAGCACTGGGCAGTGCCCATGCCTTCCCCATGTCCAAG GCCTCAACTGTGACCATTGTGCCCCCAACTTTTGGAACTTCACCAGTGGCCGTGGCTGCCAGCCTTGTGC TTGTCACCCAAGCCGGGCCAGAGGCCCTACCTGCAATGAGTTCACAGGGCAGTGTCACTGTCATGCTGGC TTTGGTGGGAGGACTTGTTCTGAGTGCCAAGAGCTCTACTGGGGAGACCCTGGTCTGCAGTGCCGTGCCT GTGACTGTGATCCTAGAGGAATAGACAAACCTCAGTGTCATCGTTCCACAGGCCACTGTAGCTGCCGCCC AGGCGTGTCTGGTGTGCGCTGTGACCAGTGTGCTCGTGGCTTCTCAGGGGTTTTTCCTGCTTGTCACCCC TGCCACGCTTGCTTTGGAGACTGGGATCGTGTGGTACAGGACCTGGCTGCTCGGACGCGGCGCCTGGAGC AGTGGGCTCAGGAGTTGCAGCAAACAGGAGTGCTGGGTGCCTTTGAGAGCAGCTTTTTGAACATGCAGGG GAAGCTAGGCATGGTGCAGGCCATTATGAGTGCCCGCAATGCCTCAGCCGCCTCTACGGCGAAGCTTGTA GAGGCCACAGAGGGACTACGTCATGAAATCGGGAAGACCACCGAGCGCCTGACTCAGTTAGAAGCAGAGC TAACAGCTGTGCAGGATGAGAACTTCAATGCCAACCATGCACTCAGTGGTCTGGAGAGAGACGGGCTTGC GCTTAATCTCACCCTGAGGCAGCTGGATCAGCATCTGGAGATCCTCAAACATTCAAATTTCTTAGGTGCC TATGACAGCATCCGACATGCCCACAGCCAGTCCACAGAGGCAGAGCGCCGTGCCAACGCCTCCACCTTTG CAGTACCCAGCCCTGTGAGCAACTCAGCAGATACCCGGCGTCGGACGGAAGTGCTAATGGGTGCCCAAAA AGAAAACTTCAACCGCCAACATTTGGCCAACCAGCAGGCACTGGGACGGCTCTCTGCACATGCCCACACC CTGAGCCTGACGGGCATAAATGAGTTGGTGTGTGGGGCACCAGGGGACGCACCCTGTGCCACCAGCCCTT GTGGGGGTGCCGGATGTCGGGATGAAGATGGGCAGCCCCGTTGTGGTGGCCTCGGTTGCAGTGGGGCAGC AGCCACGGCAGATCTAGCGCTGGGCCGGGCTCGGCACACGCAGGCAGAGCTGCAGCGGGCACTGGTAGAA GGTGGCGGCATCCTCAGCCGGGTGTCTGAGACTCGTCGGCAGGCAGAAGAGGCACAGCAGCGAGCACAGG CAGCCCTGGACAAGGCTAATGCTTCCAGGGGCCAGGTGGAACAGGCCAATCAGGAGCTTCGAGAACTTAT CCAGAATGTGAAAGACTTCCTCAGCCAGGAGGGAGCCGATCCTGACAGTATTGAAATGGTAGCGACTCGG GTGCTAGACATCTCCATCCCGGCCTCACCCGAGCAGATCCAGCGCCTAGCCAGTGAGATTGCAGAACGCG TCCGAAGCCTGGCCGACGTGGACACAATCCTGGCCCATACCATGGGCGACGTGCGTCGGGCTGAACAGCT ACTGCAAGATGCGCACCGGGCACGGAGCCGGGCCGAGGGTGAGAGACAGAAGGCAGAGACAGTCCAAGCG GCACTGGAGGAGGCTCAGAGGGCACAAGGAGCTGCTCAGGGTGCCATCTGGGGAGCAGTGGTTGACACAC AAAACACAGAGCAGACCCTGCAGCGGGTCCAGGAGAGGATGGCAGGTGCAGAGAAGTCTCTGAACTCTGC CGGTGAGCGGGCTCGGCAATTAGACGCCCTCCTGGAGGCCCTGAAACTGAAACGGGCAGGAAATAGCCTG GCAGCATCTACAGCGGAAGAAACAGCAGGCAGTGCCCAGAGCCGTGCCAGGGAGGCTGAGAAACAACTAC GGGAACAAGTAGGTGACCAATACCAAACAGTGAGGGCGTTGGCAGAGCGGAAGGCTGAAGGTGTTCTGGC TGCACAAGCCAGGGCAGAACAACTGCGGGATGAGGCTCGGGACCTGTTGCAGGCCGCTCAGGATAAGCTG CAGCGGCTACAGGAGCTGGAGGGCACATATGAGGAGAACGAGCGTGCACTGGAGGGCAAAGCGGCCCAGC TGGATGGGCTGGAAGCCAGGATGCGCAGTGTGCTCCAGGCCATCAACTTGCAGGTCCAGATCTACAACAC CTGCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212084 representing NM_008483 Red=Cloning site Green=Tags(s) MEWASGEPGRGRQGQPLPWELRLGLLLSVLAATLAQAPSLDVPGCSRGSCYPATGDLLVGRADRLTASST CGLHSPQPYCIVSHLQDEKKCFLCDSRRPFSARDNPNSHRIQNVVTSFAPQRRTAWWQSENGVPMVTIQL DLEAEFHFTHLIMTFKTFRPAAMLVERSADFGRTWHVYRYFSYDCGADFPGIPLAPPRRWDDVVCESRYS EIEPSTEGEVIYRVLDPAIPIPDPYSSRIQNLLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYALYEL VIRGNCFCYGHASQCAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYQDLPWHPAEDGHTHACRKC ECNGHTHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCEFCRPFFYRDPTKDMRDPAVCRPCDCDPMGS QDGGRCDSHDDPVLGLVSGQCRCKEHVVGTRCQQCRDGFFGLSASDPRGCQRCQCNSRGTVPGSSPCDSS SGTCFCKRLVTGHGCDRCLPGHWGLSHDLLGCRPCDCDVGGALDPQCDEATGQCRCRQHMIGRRCEQVQP GYFRPFLDHLTWEAEAAQGQGLEVVERLVTNRETPSWTGPGFVRLREGQEVEFLVTSLPRAMDYDLLLRW EPQVPEQWAELELMVQRPGPVSAHSPCGHVLPKDDRIQGMLHPNTRFLVFPRPVCLEPGISYKLKLKLIG TGGRAQPETSYSGLLIDSLVLQPHVLVLEMFSGGDAAALERRTTFERYRCHEEGLMPSKAPLSETCAPLL ISVSALIYNGALPCQCDPQGSLSSECSPHGGQCRCKPGVVGRRCDVCATGYYGFGPAGCQACQCSPDGAL SALCEGTSGQCPCRPGAFGLRCDHCQRGQWGFPNCRPCVCNGRADECDTHTGACLGCRDYTGGEHCERCI AGFHGDPRLPYGGQCRPCPCPEGPGSQRHFATSCHRDGYSQQIVCQCREGYTGLRCEACAPGHFGDPSKP GGRCQLCECSGNIDPMDPDACDPHTGQCLRCLHNTEGPHCGYCKPGFHGQAARQSCHRCTCNLLGTDPRR CPSTDLCHCDPSTGQCPCLPHVQGLNCDHCAPNFWNFTSGRGCQPCACHPSRARGPTCNEFTGQCHCHAG FGGRTCSECQELYWGDPGLQCRACDCDPRGIDKPQCHRSTGHCSCRPGVSGVRCDQCARGFSGVFPACHP CHACFGDWDRVVQDLAARTRRLEQWAQELQQTGVLGAFESSFLNMQGKLGMVQAIMSARNASAASTAKLV EATEGLRHEIGKTTERLTQLEAELTAVQDENFNANHALSGLERDGLALNLTLRQLDQHLEILKHSNFLGA YDSIRHAHSQSTEAERRANASTFAVPSPVSNSADTRRRTEVLMGAQKENFNRQHLANQQALGRLSAHAHT LSLTGINELVCGAPGDAPCATSPCGGAGCRDEDGQPRCGGLGCSGAAATADLALGRARHTQAELQRALVE GGGILSRVSETRRQAEEAQQRAQAALDKANASRGQVEQANQELRELIQNVKDFLSQEGADPDSIEMVATR VLDISIPASPEQIQRLASEIAERVRSLADVDTILAHTMGDVRRAEQLLQDAHRARSRAEGERQKAETVQA ALEEAQRAQGAAQGAIWGAVVDTQNTEQTLQRVQERMAGAEKSLNSAGERARQLDALLEALKLKRAGNSL AASTAEETAGSAQSRAREAEKQLREQVGDQYQTVRALAERKAEGVLAAQARAEQLRDEARDLLQAAQDKL QRLQELEGTYEENERALEGKAAQLDGLEARMRSVLQAINLQVQIYNTCQ SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_008483
ORF Size	5397 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008483.2, NP_032509.2
RefSeq Size	5624 bp
RefSeq ORF	5400 bp
Locus ID	16779
UniProt ID	Q61292
MW	197 kDa
Gene Summary	Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.[UniProtKB/Swiss-Prot Function]

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