Tln2 (BC079896) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5730421P04Rik; AI507121; AI787438; AL118320; mKIAA0320
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212081 representing BC079896 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGTCAACTATGTTGGAAGAATCAGTTTCCCCAAAAAAAATCTACCATCCTGCAGCAGCAGTTCA ACCGGACTGGGAAAGCAGAGCATGGTTCGGTAGCACTGCCAGCTGTCATGCGCTCTGGCTCCAGTGGGCC TGAGACGTTCAATGTTGGCAGCATGCCGTCACCACAGCAGCAGGTCATGGTCGGACAGATGCACCGAGGA CACATGCCTCCGCTGACGTCGGCACAGCAAGCCCTTATGGGGACCATCAACACAAGCATGCACGCTGTCC AGCAGGCTCAGGATGACCTCAGCGAGCTCGACTCGCTGCCACCCCTCGGCCAAGATATGGCATCTAGGGT ATGGGTTCAGAACAAAGTGGATGAATCCAAGCATGAAATCCACTCTCAAGTCGATGCTATCACAGCTGGG ACAGCTTCAGTTGTCAACCTCACAGCTGGTGACCCTGCAGACACTGACTACACAGCCGTGGGCTGTGCAA TCACTACCATTTCTTCCAACCTGACCGAAATGTCCAAGGGGGTCAAGCTGCTGGCAGCCCTCATGGACGA TGACGTGGGCAGTGGGGAGGATCTTCTCAGAGCTGCCAGGACCCTCGCCGGGGCTGTGTCTGATTTGCTG AAGGCTGTGCAGCCTACTTCAGGAGAGCCTCGACAGACTGTTTTGACTGCTGCCGGAAGCATCGGCCAAG CCAGTGGAGATCTCCTCAGACAGATTGGAGAGAACGAGACGGATGAGAGATTCCAAGATGTTTTAATGAG TCTAGCTAAGGCTGTTGCCAATGCTGCTGCCATGTTAGTACTGAAGGCGAAGAATGTGGCCCAGGTGGCT GAAGACACCGTCCTACAGAACAGGGTGATCGCCGCAGCCACCCAGTGTGCCCTCTCTACCTCGCAGCTTG TGGCGTGTGCTAAGGTGGTGAGCCCCACCATCAGCTCTCCCGTGTGCCAGGAGCAGCTGATCGAAGCTGG GAAGCTGGTGGACCGCTCTGTGGAAAACTGTGTCCGAGCCTGCCAGGCGGCCACCAGTGATAGCGAGCTC CTGAAGCAGGTCAGCGCGGCGGCCAGTGTGGTCAGCCAGGCCCTGCACGACCTCCTGCAGCATGTGCGGC AGTTCGCCAGCCGAGGCGAGCCCATTGGCCGCTATGACCAGGCCACCGACACCATCATGTGTGTCACTGA GAGCATCTTCAGTTCCATGGGCGATGCTGGTGAGATGGTGCGCCAAGCCCGGGTGCTAGCCCAGGCCACA TCAGATCTCGTCAATGCCATGAGGTCAGATGCGGAGGCTGAGATTGACATGGAGAATTCCAAGAAGCTCC TGGCGGCCGCAAAGCTCTTGGCTGACTCCACTGCCCGCATGGTGGAAGCTGCGAAGGGGGCTGCAGCCAA CCCAGAGAATGAAGACCAGCAGCAGAGGCTGAGAGAAGCTGCCGAGGGTCTCCGAGTAGCAACCAATGCC GCTGCGCAGAATGCTATTAAGAAAAAAATCGTCAACCGGCTGGAGGTTGCAGCCAAGCAGGCTGCAGCTG CTGCCACACAGACCATCGCAGCCTCCCAGAATGCAGCCATCTCCAACAAGAACCCCTCAGCCCAGCAACA ACTGGTCCAGAGCTGCAAGGCGGTGGCTGATCACATCCCTCAGCTAGTGCAGGGCGTGAGAGGGAGCCAA GCACAAGCAGAAGACCTCAGTGCCCAGCTGGCTCTCATCATCTCCAGCCAGAACTTCCTGCAGCCTGGAA GCAAGATGGTGTCCTCTGCTAAGGCGGCGGTGCCCACTGTGAGTGACCAGGCTGCAGCCATGCAGTTGAG TCAGTGTGCCAAGAACCTGGCAACCAGCCTGGCCGAGCTGCGCACAGCCTCGCAGAAGGCCCACGAAGCC TGTGGCCCCATGGAAATAGATTCTGCACTGAACACTGTGCAGACTCTCAAGAATGAGCTGCAGGACGCCA AGATGGCCGCTGCAGAAAGTCAACTGAAACCACTTCCAGGAGAAACGCTGGAAAAGTGTGCTCAGGACCT GGGAAGCACCTCTAAGGGAGTGGGCTCCTCCATGGCACAGCTCCTGACCTGTGCTGCTCAAGGCAATGAG CATTACACAGGCGTGGCAGCAAGAGAAACAGCCCAGGCCCTGAAAACACTGGCCCAGGCTGCCCGGGGAG TGGCGGCATCCACAAATGATCCTGAGGCTGCTCATGCCATGTTAGACTCTGCTCGGGATGTGATGGAGGG CTCTGCCATGCTCATCCAGGAAGCCAAGCAGGCACTAATTGCACCTGGAGACACAGAGAGTCAGCAGAGA TTAGCCCAGGTGGCCAAAGCTGTGTCTCACTCACTGAATAACTGCGTGAATTGCCTTCCTGGACAGAAGG ATGTGGATGTCGCCTTGAAGAGCATCGGGGAGGCCAGCAAGAAGCTACTTGTAGATTCGCTACCTCCAAG CACAAAGCCCTTCCAGGAGGCCCAGAGTGAGCTGAACCAGGCTGCTGCTGATCTGAACCAATCTGCTGGG GAAGTCGTCCATGCCACAAGGGGCCAGAGTGGAGAGCTGGCAGCCGCTTCTGGAAAATTCAGTGATGACT TTGACGAGTTCCTGGATGCCGGCATTGAGATGGCTGGCCAGGCACAGACAAAAGAAGACCAGATGCAAGT GATAGGGAACCTGAAGAATATCTCGATGGCGTCCAGCAAGCTCCTGCTGGCCGCCAAGTCTCTCTCTGTA GATCCTGGAGCTCCCAACGCAAAAAATCTCCTGGCTGCAGCTGCAAGAGCTGTGACAGAGAGCATCAATC AGCTCATCATGCTGTGTACTCAGCAGGCCCCTGGGCAGAAGGAGTGTGACAACGCCCTGCGTGAGCTCGA GACTGTCAAGGGGATGTTGGAAAACCCGAATGAGCCTGTGAGCGACCTCTCTTATTTTGACTGCATTGAG AGTGTGATGGAGAACTCCAAGGTTCTGGGCGAGTCAATGGCAGGGATTTCACAGAATGCCAAGACTGGAG GTAACCCCAAGGCACAGCACACCCACGACGCCATCACAGAGGCTGCGCAGCTGATGAAGGAAGCTGTGGA TGACATCATGGTGACACTGAATGAAGCAGCCAGTGAGGTGGGACTGGTGGGAGGCATGGTGGACGCCATC GCAGAGGCTATGAGCAAGCTGGATGAAGGCACACCTCCAGAACCAAAGGGAACGTTTGTTGACTACCAGA CGACTGTGGTTAAATACTCCAAAGCCATTGCCGTCACAGCTCAGGAAATGATGACTAAGTCGGTTACTAA CCCGGAGGAGTTGGGAGGCCTGGCTTCACAAATGACCACTGACTATGGGCACCTGGCTCTCCAGGGCCAG ATGGCAGCAGCCACCGCCGAACCAGAGGAGATTGGATTCCAGATTCGCACACGTGTGCAGGACCTTGGCC ATGGCTGTATCTTCCTGGTGCAAAAGGCAGGAGCCCTCCAGGTGTGCCCCACAGACAGCTACACCAAGAG GGAGCTGATCGAGTGTGCCCGCTCTGTCACTGAGAAGGTGTCCTTGGTGCTGTCTGCTCTCCAGGCTGGA AACAAGGGGACCCAGGCATGCATCACGGCCGCAACTGCAGTTTCTGGGATCATTGCCGATCTGGACACCA CCATTATGTTTGCAACAGCTGGCACACTGAATGCAGAGAACGGCGAGACCTTTGCCGATCACAGGGAGAA CATTCTGAAGACAGCCAAGGCCTTGGTGGAAGACACAAAGCTCCTCGTGTCTGGGGCTGCATCCACTCCA GACAAGCTGGCCCAGGCCGCTCAGTCCTCAGCAGCCACCATCACCCAGCTGGCAGAGGTGGTCAAGCTGG GGGCCGCCAGCCTGGGCTCCAATGACCCTGAGACCCAGGTGGTACTGATAAATGCCATCAAAGACGTGGC CAAGGCCCTTTCCGATCTCATTGGTGCTACCAAGGGAGCTGCCAGCAAGCCGGCTGACGACCCCTCCATG TACCAGCTCAAAGGAGCCGCCAAGGTGATGGTGACCAATGTCACCTCCCTTCTTAAGACTGTGAAGGCAG TGGAAGACGAGGCCACCCGGGGCACAAGGGCACTTGAGGCCACCATCGAGTACATAAAACAGGAGCTCAC AGTGTTCCAGTCAAAAGACATACCTGAAAAGACATCATCACCCGAGGAATCAATAAGAATGACGAAAGGT ATCACCATGGCAACAGCGAAAGCCGTGGCAGCTGGGAACTCATGTAGACAAGAGGATGTAATTGCAACCG CCAATCTGAGCAGGAAGGCTGTCTCAGATATGTTGATAGCTTGCAAGCAAGCATCCTTCTACCCTGATGT CAGTGAAGAGGTGCGAACCAGAGCCTTGCGGTATGGCACAGAATGCACCTTGGGCTACCTGGACCTACTA GAACACGTCTTGGTGATCCTTCAAAAGCCAACCCCGGAACTCAAGCATCAGCTGGCTGCTTTCTCCAAGA GAGTTGCTGGAGCTGTGACGGAGCTCATCCAGGCAGCGGAAGCCATGAAAGGAACAGAGTGGGTGGATCC AGAAGACCCAACCGTCATTGCAGAAACTGAGTTACTGGGAGCTGCAGCATCCATTGAAGCCGCTGCCAAG AAGTTAGAGCAGCTGAAGCCAAGAGCAAAGCCAAAGCAAGCAGACGAGACCCTGGATTTTGAAGAACAGA TCTTAGAAGCTGCTAAATCCATTGCTGCTGCCACAAGTGCCCTGGTCAAATCGGCCTCAGCAGCCCAGAG GGAGCTGGTAGCCCAAGGCAAGGTGGGCTCCATCCCTGCCAATGCTGCCGATGATGGACAGTGGTCCCAG GGGCTGATCTCTGCCGCCCGGATGGTGGCGGCAGCAACCAGCAGTCTCTGTGAGGCAGCCAATGCCTCTG TGCAGGGACATGCCAGTGAGGAAAAGCTCATCTCATCCGCCAAGCAGGTCGCAGCTTCAACCGCTCAGCT GCTGGTGGCCTGCAAGGTGAAGGCCGACCAGGATTCAGAGTCTATGAAGCGGCTACAGGTAATGGTCACT GATGCTGGTGGGAAAATACTCCTGTTGGAGCGGGCGGCAGGAAATGCTGTGAAAAGAGCCTCAGACAATC TTGTTCGTGCAGCCCAGAAGGCAGCATTTGGCAAAGCTGATGATGACGATGTTGTGGTGAAAACCAAGTT CGTGGGAGGCATTGCTCAGATCATTGCAGCCCAGGAGGAAATGCTGAAGAAAGAACGAGAGCTGGAGGAA GCCAGGAAGAAGCTGGCCCAGATCCGCCAGCAGCAGTACAAATTCCTCCCCACAGAGCTGAGGGAAGATG AGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212081 representing BC079896 Red=Cloning site Green=Tags(s) MRSQLCWKNQFPQKKSTILQQQFNRTGKAEHGSVALPAVMRSGSSGPETFNVGSMPSPQQQVMVGQMHRG HMPPLTSAQQALMGTINTSMHAVQQAQDDLSELDSLPPLGQDMASRVWVQNKVDESKHEIHSQVDAITAG TASVVNLTAGDPADTDYTAVGCAITTISSNLTEMSKGVKLLAALMDDDVGSGEDLLRAARTLAGAVSDLL KAVQPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMSLAKAVANAAAMLVLKAKNVAQVA EDTVLQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVENCVRACQAATSDSEL LKQVSAAASVVSQALHDLLQHVRQFASRGEPIGRYDQATDTIMCVTESIFSSMGDAGEMVRQARVLAQAT SDLVNAMRSDAEAEIDMENSKKLLAAAKLLADSTARMVEAAKGAAANPENEDQQQRLREAAEGLRVATNA AAQNAIKKKIVNRLEVAAKQAAAAATQTIAASQNAAISNKNPSAQQQLVQSCKAVADHIPQLVQGVRGSQ AQAEDLSAQLALIISSQNFLQPGSKMVSSAKAAVPTVSDQAAAMQLSQCAKNLATSLAELRTASQKAHEA CGPMEIDSALNTVQTLKNELQDAKMAAAESQLKPLPGETLEKCAQDLGSTSKGVGSSMAQLLTCAAQGNE HYTGVAARETAQALKTLAQAARGVAASTNDPEAAHAMLDSARDVMEGSAMLIQEAKQALIAPGDTESQQR LAQVAKAVSHSLNNCVNCLPGQKDVDVALKSIGEASKKLLVDSLPPSTKPFQEAQSELNQAAADLNQSAG EVVHATRGQSGELAAASGKFSDDFDEFLDAGIEMAGQAQTKEDQMQVIGNLKNISMASSKLLLAAKSLSV DPGAPNAKNLLAAAARAVTESINQLIMLCTQQAPGQKECDNALRELETVKGMLENPNEPVSDLSYFDCIE SVMENSKVLGESMAGISQNAKTGGNPKAQHTHDAITEAAQLMKEAVDDIMVTLNEAASEVGLVGGMVDAI AEAMSKLDEGTPPEPKGTFVDYQTTVVKYSKAIAVTAQEMMTKSVTNPEELGGLASQMTTDYGHLALQGQ MAAATAEPEEIGFQIRTRVQDLGHGCIFLVQKAGALQVCPTDSYTKRELIECARSVTEKVSLVLSALQAG NKGTQACITAATAVSGIIADLDTTIMFATAGTLNAENGETFADHRENILKTAKALVEDTKLLVSGAASTP DKLAQAAQSSAATITQLAEVVKLGAASLGSNDPETQVVLINAIKDVAKALSDLIGATKGAASKPADDPSM YQLKGAAKVMVTNVTSLLKTVKAVEDEATRGTRALEATIEYIKQELTVFQSKDIPEKTSSPEESIRMTKG ITMATAKAVAAGNSCRQEDVIATANLSRKAVSDMLIACKQASFYPDVSEEVRTRALRYGTECTLGYLDLL EHVLVILQKPTPELKHQLAAFSKRVAGAVTELIQAAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAK KLEQLKPRAKPKQADETLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGSIPANAADDGQWSQ GLISAARMVAAATSSLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSESMKRLQVMVT DAGGKILLLERAAGNAVKRASDNLVRAAQKAAFGKADDDDVVVKTKFVGGIAQIIAAQEEMLKKERELEE ARKKLAQIRQQQYKFLPTELREDEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC079896
ORF Size	5325 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC079896.1
RefSeq Size	6238 bp
RefSeq ORF	5327 bp
Locus ID	70549
MW	228.7 kDa
Gene Summary	As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity (By similarity).[UniProtKB/Swiss-Prot Function]

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