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Cc2d2a (NM_172274) Mouse Tagged ORF Clone
CAT＃: MR212058
- TrueORF®
Cc2d2a (Myc-DDK-tagged) - Mouse coiled-coil and C2 domain containing 2A (Cc2d2a)
ORF Plasmid: tGFP
"NM_172274" in other vectors (4)
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CNY 11,176.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730509K17Rik; b2b1035Clo |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212058 representing NM_172274
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATACCAACGACGAAAAAATGAAAATAATTTCAGAGGACTTCACTGGAGATGGTGTGGACACAGAGG CGGGAAGACGGAAGAAGAATTCAAAGGTGCGAAGACAGCAGAGAAAGAAAAAGCCACCCGCCTCTCCCCC TGAGGAAATGGTGTCAGACAAATTCCAAGATGGTGGGCAGCAGGAAGTGGTGGAGGAAGAGCCCGAGACC AACCTCCTGAGCTTGACAGCCCGGCGGGGGCCCCGGAGCTTACCTCCCATCCCTTCAGCTTCTAGAACCG GCTTTGCAGAATTTTCCATGAGGGAACGAATGAGGGAGAAACTTCAGGCTGCAAGGTCCAAGGCAGAAAG CGCATTGTTGCGGGATGTCCCCACCCCTAGACCCAGGCGCCTGCGCAGTCCCTCCAGAGAGAAGGAAACA GAGACTGAATTTGGCACAGAGCCAAGTACAGAGGTGCAAGACACTCAAAAAGAAGACGACACAAAGAGTT ATTCAAGAATTAAGTTCCGTGATTCTGTACGAAAAATTAAGTCAAAACCCCAGCTCCCTCCTGGTTTCCC ATCTGCAGAAGAGGCCTATAATTTCTTTACGTTCAACTTTGATCCTGAGCCAGAGGAATCAGAGGAAAAA TCACCAGTAAAAGGTGGAGAGAGAGCTCATCATGAGGACCAAGAAGGAGAAGAAGGAACACAAGCTCAAG AGAGAGCGAAGAAAACGGAAGAGGAAGAGCTGCTTAATGGGAAGGATGCTGAAGACTTCCTGTTGGGCTT AGATCCCACGGCCCATGACTTCGTGGCAGTCAGAGCTGCAGAGTACAAGAGTGCCCGCATTCAGCTGCAG AAGGAGAAAGAAATCCTCTTCACGCCTAGTCGGCTGACAGTGCCGACCTATAAAAAGCTTCCTGAGAACA TACAGCCAAGATTCCTGGAAGATGAAGGTCTTTACATCGGGGCCAGACCAGAGGTGGCACGCACCAATGA GAACATCATGGAGAACCGACTGCTGATACAGGAGCCTGGGAGTAAGTGGTTCGGAGATGATGGCAGAATC CTGGCACTGCCCAGCCCCATCAAGCCGTTTCCCTCAAGGCCATCACTAACCACACGGGAGCAAAGCCCCA AGGCTGGACTGGAGACTTTGTACAAGAAGGCAGAGAAGTATGTCCACAGTAGACAGCATATGATTGGATC TGGAGACCCTCCAGGAAATTTCCAACTGGACATCGATATTTCTGGCTTAATCTTCACTCATCACCCCTGT TTTAGCCGAGAGCATGTCTTGGCATCAAAGCTGGCCCAGTTGTATGACCAGTACCTTGCAAGACAGCAAA GAAATAAGACGAAATTCCTTACAGATAAGCTTCAAGCACTAAGAAAAGCTGTTCAGACCAGTCTTAACCC AGAGAAACCTCATCAGTCTCTTGACACAACCCAGAAAACCATCAATGAGTATAAATCTGAAATTCGGCAG ACTCGAAAACTGCGAGATGCTGAACAAGAGAAAGATCGAACACTGCTGAAGACCATCATAAAGGTCTGGA AAGAGATGAAGTCATTACGGGAGTTCCAGAGGTTTACAAACACGCCGTTGAAGCTCGTCTTGAGAAAGGA AAAAGTTGACCCGAAACTAGATGAGGATGCATATGAAGCCGAGATTCAAGCTGAGATCCATGAACTGCTG GAGGAACACATGGAGGAATATGCAACAAAGATGGAAGAGTACAGAACATCTCATCAGCAATGGAAAGCCT GGAGGAAAGCCCAGAGGGCCAAAAAGAAGAAAAAGAAGCAAACAACAGAGGAACACCTCGAGGAGGAAGA GGCTGAGGAGTCCTTCCCTGAGGAAGAGGTTACTAAGCCCATTCCCCCAGAGCCCACTGACCCAGCTGTC ATAGAGCAGCAGGTGCGGGAGAGAGCAGCCCACAGCAGGAGGAGGCCAGGGGAGCCCACACTCATTCCAG AGTTGAGTCTGGCAGGGAATGTGACCCCCAATGACCAGTGTCCTAGGGTGGAGGTCTCCAGAAGGGAAGA TGTCAGGAGGCGCTCTGTATACTTAAAGGTGGTCTTCAACAGCAAAGAGGTGTCGAGGACTGTTAGCCGG CCTCTAGGGGCAGATTTCCGGGTTCACTTTGGACAGATTTTCAACCTGCAGATATTCAACTGGCCAGAGA GCTTAATGCTTCAGGTCTATGAAACTATTGGTCATAGTGGTACCACCTTGCTAGCTGAGGTCTTCCTGCC TATCCCTGAGACGACCCTTGTCACGGGAAGGGCCCCCATTGAAGAGGTGGAGTTCAGCAGTAACCAGCAT GTGACATTAGACCATGAGGGAGTTGGAAGTGGGGTGCCCTTCTCCTTCGAAGCTGATGGCAGCAATCAAC TGACTCTAATGACATCAGGGAAAGTGTCCCACAGTGTGGCATGGGCCGTTGGGGAAAATGGGATTCCTTT AATTCCTCCACTGTCACAGCAGAACATTGGATTTCGAAGTGCTTTGAGGAGGGCAGATGCCATCTCATCC ATTGGCACATCAGGGCTGACGGACATGAAGAAGTTGGCCAAGTGGGCAGCAGAGTCCAAGCTTGACCCCA ATGATCCCAACCACGCCCCATTGATGCAGCTGATCTCGGTTGCTACCAGTGGTGAATCCTATGTCCCTGA CTTCTTCCGGCTGGAGCAGCTGCAGCAGGAATTCAACTTCGTCTCAGAGGAGGAATTAAATAGATCCAAA CGGTTCCGACTTCTCCATCTTAGGAGCCAAGAGGTGCCAGAATTCCGGAACTATAAACAAATTCCAGCAT ATGACCGAGAAATTATGGAAAAGGTATTCCAGGACTATGAGAAACGATTACGAGACAGAAATGTAATTGA AACCAAGGACCACCTGGACATGCACAGAGCCACAGTAGCCAAGTACCTTCAGCAAGTTAGAGAAGCAGTG GTAAATCGCTTCCTAACTGCCAAACACCATTTTCTTCTTACTGATTTGGTAGTAGAAGAAGAAGTCCCCA ATATCAGTTCTGAAGGCTCGGGCATTTTGGGCCTAAGCCTTTTCAAGCTGGCAGAACAAAAGCGACCACT GAGGCCACGGAGGAAAGGTCGGAAGAAGGTGACAGCCCAAAACCTGTCTGATGGAGACATAAAGCTGCTG GTGAACATTATCCGGGCTTACGACATCCCTGTAAGGAAACCTGTAGTGAGCAAGTTTCAGCAGCCATCGA GATCTTCAAGAACTTTCAGTGAAAAGCAAACTGCTTCCCCCAGCACACACAGCCCACTACACAACGCTGA CTACCCCCTCGGCCAGGTCTTAGTCCGGCCTTTTGTGGAGGTCTCTTTTCAAAGAACAATTTGCCACACA ACCACAGCTGAAGGACCAAATCCCAGCTGGAACGAAGAACTTGAACTTCCGTTCAGGGCCCCCAATGGGG ACTACAGCACAGCCAGCTTACAGTCAGTGAAGGATGACGTATACATTAACATTTTTGATGAAGTGCTGTA TGATATCCTAGAGGATGACCGTGAAAGAGGAAGTGGGATCCATACGCGTATTGAGAGACACTGGTTAGGA TGTGTGAAAATCCCATTTAGCACCATCTATTTCCAGGCAAGGATTGATGGCACATTTAAAATAGACATTC CTCCAGTTCTCCTGGGCTATAGTAAGGAGCGAAACATTATTATGGAACGAGCCTTTGATTCTGCCCGAAG CTTGAGTGAAGGCTCCTACATCACCCTGTTTATTACCATTGAGCCTCAGCTGGTTCCTGGGGAGCCTATG AGAGAAAAGATGAGTGACATGCTTAAGAAGTTTGATACCCAGGAAGATGAGAAATTACTTCAAGCAACAG AGAAATTTCAAGCTGAATGTGCCTTAAAATTCCCCCAGCGTCAGTGCCTCACCACAGTGACTGACATGAC CGGGAAAACGGTTTTTATTACGCGTTATCTCAAGCCTTTAAACCCTCCGCAGGAGCTCCTTCACGTCTAC CCCAACAACCCGCAGGCCACTGCGGAGCTGGTAGCTCGGTATGTGTCCTTGATCCCTTTCTTGCCTGATA GCGTGTCGTTTGCTGGAGTCTGTGACCTGTGGAGCACTTCTGATCAATTCCTTGACCTCCTGGCGGGTGA TGAAGAAGAGCATGCAGTACTGCTATGCAATTACTTTCTGTTCCTGGGTAAGAAGGCATGGCTGGTGATG GGCAGTGCGATTCCTGAGGGTCCAACTGCCTATGTGCTAACTTGGGAGAAAAATTATTATTTAATATGGA ATCCCTGCAGCGGACATTGTTACGGACAATTTGATGCATTTTGCCCTTTGAAAAGTGTGGGCTGTTTAAT TGGTCCTGATAATATTTGGTTTAATATTCAACACCATGATTCTCCACTAAGGATAAATTTTGATGTCACA AAGCCCAAACTATGGAAATCTTTTTTTTCACGAAGTCTTCCATATCCTGGCCTATCCAGTGTTCAGCCGG AAGAGCTAATTTATCAACACACAGACAAAGCAGTAGCTGCTGAACTGCAAGACAGGATTGAAAAAATACT AAAAGAAAAAATCATGGACTGGAGGCCACGCCACCTGACCAGGTGGAACAGGTACTGTACCTCCACCCTG CGCCACTTCTTGCCTCTACTGGAAAGAAGTCAAGGAGAAGATATAGAAGACGACCACAGGGCAGAGCTGT TGAAGCAGCTGGGGGACTACAGGTTCTCTGGATTTCCTCTTCACATGCCTTATTCTGAAGTGAAGCCTTT GGTTGAAGCGGTGTATAGCACTGGAGTACATAACATTGACCTTCCTAATGTTGAGTTTGCTTTAGCTGTA TACATACACCCTTATCCCAAAAACGTTTTGTCTGTTTGGATCTATGTTGCTTCTCTTGTACGGAACAGG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR212058 representing NM_172274
Red=Cloning site Green=Tags(s) MNTNDEKMKIISEDFTGDGVDTEAGRRKKNSKVRRQQRKKKPPASPPEEMVSDKFQDGGQQEVVEEEPET NLLSLTARRGPRSLPPIPSASRTGFAEFSMRERMREKLQAARSKAESALLRDVPTPRPRRLRSPSREKET ETEFGTEPSTEVQDTQKEDDTKSYSRIKFRDSVRKIKSKPQLPPGFPSAEEAYNFFTFNFDPEPEESEEK SPVKGGERAHHEDQEGEEGTQAQERAKKTEEEELLNGKDAEDFLLGLDPTAHDFVAVRAAEYKSARIQLQ KEKEILFTPSRLTVPTYKKLPENIQPRFLEDEGLYIGARPEVARTNENIMENRLLIQEPGSKWFGDDGRI LALPSPIKPFPSRPSLTTREQSPKAGLETLYKKAEKYVHSRQHMIGSGDPPGNFQLDIDISGLIFTHHPC FSREHVLASKLAQLYDQYLARQQRNKTKFLTDKLQALRKAVQTSLNPEKPHQSLDTTQKTINEYKSEIRQ TRKLRDAEQEKDRTLLKTIIKVWKEMKSLREFQRFTNTPLKLVLRKEKVDPKLDEDAYEAEIQAEIHELL EEHMEEYATKMEEYRTSHQQWKAWRKAQRAKKKKKKQTTEEHLEEEEAEESFPEEEVTKPIPPEPTDPAV IEQQVRERAAHSRRRPGEPTLIPELSLAGNVTPNDQCPRVEVSRREDVRRRSVYLKVVFNSKEVSRTVSR PLGADFRVHFGQIFNLQIFNWPESLMLQVYETIGHSGTTLLAEVFLPIPETTLVTGRAPIEEVEFSSNQH VTLDHEGVGSGVPFSFEADGSNQLTLMTSGKVSHSVAWAVGENGIPLIPPLSQQNIGFRSALRRADAISS IGTSGLTDMKKLAKWAAESKLDPNDPNHAPLMQLISVATSGESYVPDFFRLEQLQQEFNFVSEEELNRSK RFRLLHLRSQEVPEFRNYKQIPAYDREIMEKVFQDYEKRLRDRNVIETKDHLDMHRATVAKYLQQVREAV VNRFLTAKHHFLLTDLVVEEEVPNISSEGSGILGLSLFKLAEQKRPLRPRRKGRKKVTAQNLSDGDIKLL VNIIRAYDIPVRKPVVSKFQQPSRSSRTFSEKQTASPSTHSPLHNADYPLGQVLVRPFVEVSFQRTICHT TTAEGPNPSWNEELELPFRAPNGDYSTASLQSVKDDVYINIFDEVLYDILEDDRERGSGIHTRIERHWLG CVKIPFSTIYFQARIDGTFKIDIPPVLLGYSKERNIIMERAFDSARSLSEGSYITLFITIEPQLVPGEPM REKMSDMLKKFDTQEDEKLLQATEKFQAECALKFPQRQCLTTVTDMTGKTVFITRYLKPLNPPQELLHVY PNNPQATAELVARYVSLIPFLPDSVSFAGVCDLWSTSDQFLDLLAGDEEEHAVLLCNYFLFLGKKAWLVM GSAIPEGPTAYVLTWEKNYYLIWNPCSGHCYGQFDAFCPLKSVGCLIGPDNIWFNIQHHDSPLRINFDVT KPKLWKSFFSRSLPYPGLSSVQPEELIYQHTDKAVAAELQDRIEKILKEKIMDWRPRHLTRWNRYCTSTL RHFLPLLERSQGEDIEDDHRAELLKQLGDYRFSGFPLHMPYSEVKPLVEAVYSTGVHNIDLPNVEFALAV YIHPYPKNVLSVWIYVASLVRNR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172274 |
| ORF Size | 4899 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172274.2, NP_758478.1 |
| RefSeq Size | 5463 bp |
| RefSeq ORF | 4902 bp |
| Locus ID | 231214 |
| UniProt ID | Q8CFW7 |
| MW | 187.5 kDa |
| Gene Summary | Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224775 | Cc2d2a (untagged) - Mouse coiled-coil and C2 domain containing 2A (Cc2d2a), (10ug) |
CNY 20,900.00 |
|
| MG212058 | Cc2d2a (tGFP-tagged) - Mouse RIKEN cDNA 5730509K17 gene (5730509K17Rik) |
CNY 13,300.00 |
|
| MR212058L3 | Lenti ORF clone of Cc2d2a (Myc-DDK-tagged) - Mouse coiled-coil and C2 domain containing 2A (Cc2d2a) |
CNY 13,970.00 |
|
| MR212058L4 | Lenti ORF clone of Cc2d2a (mGFP-tagged) - Mouse coiled-coil and C2 domain containing 2A (Cc2d2a) |
CNY 13,970.00 |
