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Smarca2 (NM_026003) Mouse Tagged ORF Clone
CAT＃: MR212039
- TrueORF®
Smarca2 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (Smarca2), transcript variant 2
"NM_026003" in other vectors (3)
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CNY 15,770.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610209L14Rik; brahma; brm; SNF2alpha; Snf2l2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212039 representing NM_026003
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACACCCACAGACCCAGCAGCAATGCCCCATCCTGGGCCCTCCCCGGGGCCTGGACCCTCTCCTG GACCAATTCTGGGGCCTAGTCCAGGACCAGGACCATCCCCAGGTTCTGTGCACAGCATGATGGGTCCTAG TCCCGGACCTCCCAGCGTCTCACATCCTCTGTCAACGATGGGCTCTGCAGACTTCCCACAGGAAGGCATG CACCAATTACATAAGCCCATGGATGGGATACATGACAAAGGGATTGTAGAAGATGTCCACTGTGGATCCA TGAAGGGCACCAGCATGCGCCCCCCACACCCAGGAATGGGCCCTCCACAGAGCCCCATGGACCAGCACAG CCAAGGTTATATGTCACCACATCCGTCTCCTCTGGGAGCCCCGGAGCACGTCTCTAGCCCAACCCCACCC CAGATGCCACCGAGCCAGCCAGGGGCACTCATCCCAGGAGATCCGCAGGCCATGAACCAGCCTAACAGAG GTCCCTCGCCTTTCAGTCCTGTGCAGCTGCATCAGCTTCGAGCTCAGATTTTAGCTTACAAAATGTTGGC CAGGGGCCAGCCTCTCCCTGAAACTCTGCAGCTGGCAGTCCAGGGAAAAAGGACCTTGCCTGGCATGCAG CAGCAGCAGCAGCAACAACAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAACAAC AGCAGCCCCAGCAGCCTCAGCAGCAGGCTCAGGCACAGCCCCAGCAGCAGCAGCAACAGCAGCAGCAGCC AGCTCTTGTTAGCTATAATCGACCATCTGGCCCCGGGCAGGAGCTGCTACTGAGTGGCCAGAGCGCTCCG CAGAAGCTGTCAGCACCAGCACCAAGCGGCCGACCTTCACCGGCACCCCAGGCCGCCGTCCAGCCCACGG CCACAGCGGTGCCCGGGCCCTCCGTGCAGCAGCCCGCCCCAGGGCAGCCGTCTCCGGTCCTACAGCTGCA ACAGAAGCAGAGCCGCATCAGCCCCATCCAGAAACCGCAAGGCCTGGACCCGGTGGAGATCCTGCAGGAA CGAGAGTACAGACTTCAAGCTCGCATCGCTCATAGGATACAAGAACTGGAAAGTCTGCCTGGTTCCTTGC CACCAGATTTACGCACCAAAGCAACCGTGGAACTGAAAGCACTTCGCTTACTCAACTTCCAACGTCAGCT GAGACAGGAGGTGGTGGCCTGCATGCGGAGGGACACCACCCTGGAGACGGCCCTCAACTCCAAAGCATAT AAGCGGAGCAAGCGCCAGACCCTGCGTGAGGCACGCATGACAGAGAAACTGGAGAAGCAGCAGAAGATAG AACAGGAGAGGAAACGCCGGCAGAAACACCAGGAATACCTGAACAGTATTTTGCAACATGCAAAAGATTT TAAGGAATATCACCGGTCTGTGGCCGGGAAGATCCAGAAGCTCTCCAAAGCAGTGGCGACTTGGCATGCT AACACAGAAAGGGAGCAGAAGAAGGAGACGGAGCGGATCGAGAAGGAGAGAATGCGGAGGCTGATGGCCG AAGATGAAGAGGGCTACAGGAAGCTTATTGACCAAAAGAAAGACAGACGTCTCGCCTACCTATTGCAGCA GACCGATGAGTATGTCGCCAATCTGACCAACCTGGTGTGGGAGCACAAGCAGGCCCAAGCAGCCAAAGAG AAGAAGAAGAGGAGGAGGAGGAAGAAGAAGGCTGAAGAGAATGCAGAGGGAGGGGAACCTGCCCTGGGAC CAGATGGAGAGCCAATAGATGAAAGCAGCCAGATGAGTGACCTGCCTGTCAAAGTGACACACACAGAAAC TGGCAAGGTCCTCTTTGGACCAGAAGCACCCAAAGCAAGTCAGCTGGATGCCTGGCTGGAGATGAATCCT GGTTACGAAGTTGCACCCAGATCTGACAGTGAAGAGAGTGAATCGGACTACGAGGAGGAGGATGAAGAAG AAGAGTCCAGTAGGCAGGAAACCGAGGAGAAGATACTGCTGGATCCCAACAGTGAAGAAGTTTCCGAAAA GGATGCCAAGCAGATCATTGAGACTGCGAAGCAGGACGTGGACGACGAATACAGCATGCAGTACAGTGCC AGAGGCTCTCAGTCCTACTACACGGTGGCTCACGCTATCTCTGAGAGGGTGGAGAAGCAGTCTGCCCTCC TCATTAACGGCACCCTAAAGCATTACCAGCTCCAGGGCCTGGAATGGATGGTTTCCCTGTATAATAACAA TCTGAACGGAATCTTAGCTGATGAAATGGGGCTAGGCAAGACCATCCAGACCATTGCACTCATCACGTAT CTGATGGAGCACAAAAGGCTCAATGGTCCCTACCTCATCATCGTCCCCCTCTCGACTCTGTCTAACTGGA CATATGAATTTGACAAATGGGCTCCTTCTGTGGTGAAAATTTCTTACAAGGGTACCCCTGCCATGCGACG CTCCCTCGTTCCCCAGCTACGGAGTGGCAAATTCAATGTCCTCCTGACTACTTACGAGTACATTATAAAA GACAAGCACATTCTTGCAAAGATTCGGTGGAAGTACATGATCGTGGACGAAGGCCACCGGATGAAGAATC ACCACTGCAAGCTAACCCAGGTCCTGAACACACACTATGTGGCCCCCAGGCGGATCCTTCTGACTGGGAC CCCACTGCAGAATAAGCTTCCGGAACTCTGGGCCCTCCTCAACTTCCTCCTCCCTACAATCTTCAAGAGT TGCAGCACATTTGAGCAGTGGTTTAATGCTCCATTTGCCATGACCGGTGAAAGGGTGGACCTGAACGAAG AAGAAACGATTTTGATCATCAGGCGTCTACACAAGGTGCTGAGACCCTTTTTACTGAGGAGGCTGAAGAA AGAGGTTGAGTCTCAGCTTCCGGAAAAGGTTGAGTATGTGATCAAGTGTGACATGTCAGCTCTGCAGAAG ATTCTGTACCGTCACATGCAAGCCAAGGGGATCCTCCTCACGGACGGGTCTGAGAAAGATAAGAAGGGGA AAGGAGGTGCCAAGACACTTATGAACACCATCATGCAGCTGAGAAAAATATGCAACCACCCATATATGTT TCAGCACATTGAGGAATCCTTTGCTGAACACCTGGGCTATTCGAATGGGGTCATCAATGGGGCTGAGCTG TATCGGGCCTCGGGAAAGTTTGAGCTGCTTGATCGTATTCTGCCCAAATTGAGAGCGACTAACCACCGCG TGCTGCTTTTCTGCCAGATGACGTCACTCATGACCATTATGGAGGATTACTTTGCTTTTCGGAACTTCCT GTACCTGCGCCTTGACGGCACCACCAAGTCTGAAGATCGTGCTGCTTTGCTAAAGAAGTTCAATGAACCT GGGTCCCAGTATTTCATTTTCTTGCTGAGCACAAGAGCAGGGGGCCTGGGCTTAAATCTTCAGGCGGCAG ACACGGTGGTCATATTTGACAGCGACTGGAATCCTCACCAGGATCTGCAGGCCCAAGACCGAGCTCACCG CATTGGCCAACAAAACGAGGTCCGGGTGCTGAGGCTTTGCACCGTCAACAGTGTGGAGGAAAAGATTCTC GCGGCTGCCAAGTACAAGCTGAACGTGGATCAGAAGGTTATCCAAGCAGGCATGTTTGACCAGAAGTCAT CCAGCCACGAGCGGAGGGCCTTCCTGCAGGCCATTCTGGAGCACGAGGAGGAGAATGAGGAAGAAGATGA GGTACCAGACGACGAGACCCTGAACCAGATGATTGCTCGCCGGGAGGAAGAATTTGATCTTTTTATGCGC ATGGACATGGACCGGCGGAGGGAGGATGCCCGGAACCCGAAGCGCAAACCCCGCTTGATGGAGGAAGATG AGCTGCCCTCCTGGATTATCAAGGATGACGCCGAAGTGGAAAGGCTCACCTGTGAAGAAGAGGAGGAGAA GATATTTGGGAGGGGCTCTCGCCAGCGCCGGGATGTGGACTACAGTGATGCCCTCACCGAGAAGCAATGG CTCAGGGCCATCGAAGACGGCAATTTGGAAGAAATGGAAGAGGAGGTACGGCTTAAGAAGAGAAAAAGAC GAAGAAATGTGGATAAAGACCCCGTGAAGGAAGATGTGGAAAAAGCGAAGAAAAGAAGAGGCCGCCCTCC GGCTGAGAAGTTGTCACCAAATCCCCCAAAACTAACGAAGCAGATGAACGCCATCATTGATACTGTGATA AACTACAAAGACAGTTCAGGGCGACAGCTCAGTGAAGTCTTCATTCAGTTACCTTCCAGGAAAGACTTAC CAGAATACTATGAATTAATTAGGAAGCCAGTGGATTTCAAAAAGATAAAGGAGCGAATCCGTAATCATAA GTATCGGAGCCTGGGAGACCTGGAGAAAGACGTCATGCTTCTCTGTCACAACGCACAGACATTCAACTTG GAAGGATCCCAGATCTACGAAGACTCCATTGTCCTACAGTCAGTGTTTAAGAGTGCTCGGCAGAAAATTG CCAAAGAAGAAGAGAGTGAGGAAGAAAGCAATGAAGAAGAGGAAGAAGATGATGAAGAGGAGTCGGAGTC AGAGGCGAAATCTGTGAAGGTGAAAATCAAGCTGAATAAAAAGGAAGAGAAAGGCCGGGACACAGGGAAG GGCAAGAAGCGGCCAAACCGAGGCAAAGCCAAACCCGTCGTGAGCGATTTTGACAGTGACGAGGAACAGG AAGAGAACGAACAGTCAGAAGCAAGTGGAACTGATAACGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212039 representing NM_026003
Red=Cloning site Green=Tags(s) MSTPTDPAAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPLSTMGSADFPQEGM HQLHKPMDGIHDKGIVEDVHCGSMKGTSMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPTPP QMPPSQPGALIPGDPQAMNQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGMQ QQQQQQQQQQQQQQQQQQQQQQQQQPQQPQQQAQAQPQQQQQQQQQPALVSYNRPSGPGQELLLSGQSAP QKLSAPAPSGRPSPAPQAAVQPTATAVPGPSVQQPAPGQPSPVLQLQQKQSRISPIQKPQGLDPVEILQE REYRLQARIAHRIQELESLPGSLPPDLRTKATVELKALRLLNFQRQLRQEVVACMRRDTTLETALNSKAY KRSKRQTLREARMTEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVAGKIQKLSKAVATWHA NTEREQKKETERIEKERMRRLMAEDEEGYRKLIDQKKDRRLAYLLQQTDEYVANLTNLVWEHKQAQAAKE KKKRRRRKKKAEENAEGGEPALGPDGEPIDESSQMSDLPVKVTHTETGKVLFGPEAPKASQLDAWLEMNP GYEVAPRSDSEESESDYEEEDEEEESSRQETEEKILLDPNSEEVSEKDAKQIIETAKQDVDDEYSMQYSA RGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSLYNNNLNGILADEMGLGKTIQTIALITY LMEHKRLNGPYLIIVPLSTLSNWTYEFDKWAPSVVKISYKGTPAMRRSLVPQLRSGKFNVLLTTYEYIIK DKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLLPTIFKS CSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCDMSALQK ILYRHMQAKGILLTDGSEKDKKGKGGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHLGYSNGVINGAEL YRASGKFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLRLDGTTKSEDRAALLKKFNEP GSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKIL AAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEENEEEDEVPDDETLNQMIARREEEFDLFMR MDMDRRREDARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRQRRDVDYSDALTEKQW LRAIEDGNLEEMEEEVRLKKRKRRRNVDKDPVKEDVEKAKKRRGRPPAEKLSPNPPKLTKQMNAIIDTVI NYKDSSGRQLSEVFIQLPSRKDLPEYYELIRKPVDFKKIKERIRNHKYRSLGDLEKDVMLLCHNAQTFNL EGSQIYEDSIVLQSVFKSARQKIAKEEESEEESNEEEEEDDEEESESEAKSVKVKIKLNKKEEKGRDTGK GKKRPNRGKAKPVVSDFDSDEEQEENEQSEASGTDNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026003 |
| ORF Size | 4731 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 1964 bp |
| RefSeq ORF | 711 bp |
| Locus ID | 67155 |
| MW | 27.9 kDa |
| Gene Summary | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215695 | Smarca2 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (Smarca2), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MR212039L3 | Lenti ORF clone of Smarca2 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (Smarca2), transcript variant 2 |
CNY 17,670.00 |
|
| MR212039L4 | Lenti ORF clone of Smarca2 (mGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (Smarca2), transcript variant 2 |
CNY 17,670.00 |
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