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A2m (NM_175628) Mouse Tagged ORF Clone
CAT＃: MR212004
- TrueORF®
A2m (Myc-DDK-tagged) - Mouse alpha-2-macroglobulin (A2m)
"NM_175628" in other vectors (2)
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CNY 10,088.00
货期*
现货
规格
Product images
经常一起买 (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A2mp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212004 representing NM_175628
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGCGCTGGCTCCCAAGCCTGGCCCTGCTGCCGCTGCCGCCACCGCTGCTGCTGCTGCTGTTGC TGCTGCTGCCCACAAATGCCTCAGCACCACAGAAACCAATCTACATGGTGATGGTTCCCTCCCTGCTCCA CGCTGGCACCCCTGAGAAGGGCTGCCTCCTGTTCAACCACCTAAACGAGACAGTAACAGTGAAAGTATCC ATGGAGTCTGTCCGTGGGAACCAAAGCCTCTTCACTGACCTTGTTGTTGACAAGGACTTATTCCACTGTG CCTCCTTCATCGTCCCACAGTCTTCATCCAATGAGGTGATGTTTCTCACTGTCCAAGTAAAAGGACCAAC TCATGAGTTCAGGAGGAGGAGCACGGTGCTGATCAAGACTAAAGAGAGCCTGGTCTTTGCTCAGACTGAC AAGCCCATCTACAAACCAGGACAGATGGTGAGATTTCGTGTTGTCTCATTGGACGAAAATTTCCACCCCC TTAATGAATTGATTCCTCTACTGTACATTCAGGATTCAAAAAAAAATCGCATTGCACAGTGGCAGAATTT CCGCTTAGAGGGTGGCCTCAAACAGCTGTCCTTCCCCCTCTCCTCAGAGCCCACTCAGGGCTCCTACAAG GTGGTGATACGTACAGAATCAGGCAGGACCGTCGAGCACCCCTTCTCTGTGAAGGAATTTGTGCTTCCCA AGTTTGAAGTCAAAGTTGCAGTACCAGAGACAATCACCATCCTGGAGGAAGAGATGAATGTGTCCGTGTG TGGCATATACACCTATGGGAAGCCTGTTCCAGGACACGTGACTGTGAACATTTGCAGAAAGTACAGTAAT CCTTCTAGCTGCTTTGGTGAAGAATCGCTGGCTTTCTGTGAGAAATTCAGTCAACAGCTAGATGGCCGTG GCTGCTTCTCACAGCTAGTGAAAACCAAGTCTTTCCAGCTAAAGAGGCAAGAGTATGAGATGCAGCTTGA TGTGAATGCCAAGATCCAAGAAGAAGGAACAGGTGTGGAAGAAACCGGTAAAGGGCTCACTAAGATCACA AGAACTATAACAAAACTATCATTTGTGAACGTGGATACACATTTCAGACAAGGAATTCCTTTCGTTGGGC AGGTGCTCCTGGTGGATGGGAGAGGCACCCCTATTCCATATGAAATGATCTTCATTGGAGCTGATGAAGC AAACCAGAACATAAATACTACCACTGATAAGAATGGCCTGGCACGGTTCTCCATCAACACTGATGACATC ATGGGCACGTCCCTAACTGTCAGGGCCAAATACAAAGATAGCAATGTCTGCTATGGATTTAGATGGTTGA CAGAAGAGAACGTAGAGGCTTGGCACACTGCCAACGCTGTCTTCTCACCGAGCAGAAGCTTCGTGCACCT GGAATCCCTGCCCTATAAACTGCGCTGTGAGCAAACCCTGGCGGTCCAGGCTCATTATATTCTAAATGAC GAGGCTGTGCTGGAGCGGAAGGAGTTGGTCTTCTATTATCTGATGATGGCAAAGGGAGGCATTGTTCGGG CAGGGACTCACGTTCTGCCTGTGACGCAGGGACACAAGAAAGGCCATTTCTCCATCCTCATCTCGATGGA GACAGACCTGGCTCCTGTAGCTCGACTGGTCCTTTATACCATTCTGCCCAATGGAGAAGTGGTTGGAGAT ACTGTTAAATATGAGATTGAAAAGTGCCTGGCCAACAAGGTGGATTTGGTCTTCCACCCAAATATCGGTC TTCCGGCCACACGTGCCTTCCTCAGTGTCATGGCTTCTCCTCAGTCTCTCTGTGGCCTGCGAGCTGTGGA CCAAAGCGTACTGCTCACAAAGCCTGAGGCCGAACTCTCTGCATCCCTGGTTTATGATCTGCTACCAGTG AAAGACCTCACTGGCTTCCCTAAGGGTGTGAATCAACAGGAAGAAGACACTAATGGCTGCCTTAAGCAAA ATGACACTTACATTAATGGAATCCTGTACTCCCCAGTACAGAATACAAATGAAGAGGACATGTATGGCTT CCTAAAGGATATGGGCTTAAAGGTATTCACCAACTTGAACATCCGTAAACCCAAAGTCTGTGAACGCCTT GGAGTCAATAAAATACCAGCTGCATACCACCTTGTAAGCCAAGGCCACATGGATGCTTTTCTAGAGTCTT CAGAGTCTCCCACAGAGACTACACGAAGCTACTTCCCTGAGACGTGGATCTGGGACTTGGTGATCGTGGA CTCAACAGGAGTGGCTGAAATGGAAGTGACAGTCCCTGACACCATCACTGAATGGAAGGCTGGGGCCTTC TGCCTGTCCAATGACACTGGGTTGGGCCTCTCTCCTGTGATCGATTTCCAAGCCTTCCAGCCCTTCTTCG TGGATCTCACAATGGCCTACTCCGTGATCCGTGGAGAGGCCTTCACGCTCAAGGCCACTGTGCTGAACTA CCTCCAGACATGCATCCGGGTTGGTGTACAGCTGGAGGCCTCTCCTGATTTCCTAGCTACCCCAGAGGAG AAGGAACAAAAGTCTCACTGCATCTGTATGAACGAGCGGCATACCATGTCCTGGGCGGTGATCCCCAAGT CATTAGGAAATGTGAATTTCACAGTGAGTGCAGAGGCACTGGACTCTAAGGAGCTGTGTAGGAATGAGGT GCCAGTGGTTCCTGAACGTGGAAAGAAAGACACGATCATCAAGTCCCTGCTGGTTGAACCTGAAGGTCTA GAGAATGAAGTGACATTCAACAGTTTGCTTTGTCCAACAGGTGCTGAGGTATCGGAACAGATATCTCTGA AACTGCCATCAGATGTGGTAGAGGAATCTGCCCGAGCTTCTGTCACAGTTTTGGGAGATATATTGGGTTC TGCTATGCAGAACACACAGGATCTCCTTAAGATGCCCTATGGCTGTGGAGAACAGAACATGGTTCTGTTT GCTCCTAATATCTATGTCCTGGACTATCTGAATGAAACAGAGCAGCTGACACAGGAGATCAAGACCAAGG CCATTACCTATCTCAATACGGGTTACCAAAGACAATTAAACTACAAGCACCGGGATGGCTCCTACAGTAC TTTTGGGGATAAACCTGGTAGGAGTCATGCCAATACCTGGCTCACAGCCTTTGTACTGAAGAGTTTTGCT CAGGCTCGAAGATATATCTTCATCGATGAATCACACATCACCCAAGCCCTCACCTGGCTCTCCCAGCAGC AGAAGGACAATGGCTGCTTCAGGAGCTCCGGGTCACTGCTCAACAATGCCATGAAGGGAGGAGTAGAAGA TGAAGTCACTTTGTCTGCCTACATCACCATTGCTCTCCTGGAGATGTCTCTTCCTGTCACTCATCCTGTT GTCCGAAATGCCCTCTTTTGCCTGGACACAGCCTGGAAGTCAGCGAGGAGAGGAGCCAGCGGCAACCATG TCTACACTAAGGCACTGTTGGCCTATGCATTTGCCCTTGCTGGTAACCAGGACACGAAGAAGGAGATACT GAAATCACTCGATGAGGAGGCGGTAAAAGAAGACAATTCTGTCCACTGGACCAGAGCTCAGAAACCCAGA GTGCCAGCAGATCTCTGGTACCAACCCCAGGCTCCCTCGGCTGAGGTAGAGATGACCGCTTATGTGCTCC TGGCTTATCTTACCACTGAGCTGGTTCCAACCCGAGAGGACCTGACTGCTGCAATGCTCATTGTGAAGTG GCTCACAAAGCAGCAGAATTCCCACGGTGGCTTCTCCTCCACCCAGGACACGGTAGTGGCTCTCCATGCT TTGTCCAAATACGGAGCAGCTACTTTCACAAGAGCGAAGAAAGCTGCACATGTGACCATCCAGTCTTCTG GCGCATTTTATACAAAATTCCAAGTGAACAACGACAACCAGTTATTATTACAGAGAGTCACATTGCCAAC TGTGCCTGGGGATTACACCGCAAAGGTAGCTGGAGAAGGGTGTGTCTACCTCCAGACATCCTTGAAATAT AGTGTTCTCCCGAGGGAGAAGGAGTTTCCATTTGCTTTGGTGGTGCAGACTCTGCCTGGGACTTGTGAGG ATCTCAAAGCTCACACCACCTTCCAGATCTCACTCAATATCAGTTACATTGGAAGCCGTTCTGACTCCAA CATGGCAATTGCTGATGTGAAGATGGTGTCTGGCTTCATCCCCTTGAAACCAACAGTGAAAATGCTTGAA AGATCTGTGCATGTGAGCCGGACGGAAGTCAGCAATAACCATGTCTTGATTTACTTGGATAAGGTGTCAA ATCAGATGCTGACCTTGTTCTTCATGGTGCAGCAAGATATTCCAGTGAGAGACCTGAAGCCAGCCATAGT GAAAGTCTATGATTACTACGAGAAAGATGAGTTTGCAGTTGCAAAATACAGTGCTCCCTGCAGTGCAGGT TATGGAAATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212004 representing NM_175628
Red=Cloning site Green=Tags(s) MGKRWLPSLALLPLPPPLLLLLLLLLPTNASAPQKPIYMVMVPSLLHAGTPEKGCLLFNHLNETVTVKVS MESVRGNQSLFTDLVVDKDLFHCASFIVPQSSSNEVMFLTVQVKGPTHEFRRRSTVLIKTKESLVFAQTD KPIYKPGQMVRFRVVSLDENFHPLNELIPLLYIQDSKKNRIAQWQNFRLEGGLKQLSFPLSSEPTQGSYK VVIRTESGRTVEHPFSVKEFVLPKFEVKVAVPETITILEEEMNVSVCGIYTYGKPVPGHVTVNICRKYSN PSSCFGEESLAFCEKFSQQLDGRGCFSQLVKTKSFQLKRQEYEMQLDVNAKIQEEGTGVEETGKGLTKIT RTITKLSFVNVDTHFRQGIPFVGQVLLVDGRGTPIPYEMIFIGADEANQNINTTTDKNGLARFSINTDDI MGTSLTVRAKYKDSNVCYGFRWLTEENVEAWHTANAVFSPSRSFVHLESLPYKLRCEQTLAVQAHYILND EAVLERKELVFYYLMMAKGGIVRAGTHVLPVTQGHKKGHFSILISMETDLAPVARLVLYTILPNGEVVGD TVKYEIEKCLANKVDLVFHPNIGLPATRAFLSVMASPQSLCGLRAVDQSVLLTKPEAELSASLVYDLLPV KDLTGFPKGVNQQEEDTNGCLKQNDTYINGILYSPVQNTNEEDMYGFLKDMGLKVFTNLNIRKPKVCERL GVNKIPAAYHLVSQGHMDAFLESSESPTETTRSYFPETWIWDLVIVDSTGVAEMEVTVPDTITEWKAGAF CLSNDTGLGLSPVIDFQAFQPFFVDLTMAYSVIRGEAFTLKATVLNYLQTCIRVGVQLEASPDFLATPEE KEQKSHCICMNERHTMSWAVIPKSLGNVNFTVSAEALDSKELCRNEVPVVPERGKKDTIIKSLLVEPEGL ENEVTFNSLLCPTGAEVSEQISLKLPSDVVEESARASVTVLGDILGSAMQNTQDLLKMPYGCGEQNMVLF APNIYVLDYLNETEQLTQEIKTKAITYLNTGYQRQLNYKHRDGSYSTFGDKPGRSHANTWLTAFVLKSFA QARRYIFIDESHITQALTWLSQQQKDNGCFRSSGSLLNNAMKGGVEDEVTLSAYITIALLEMSLPVTHPV VRNALFCLDTAWKSARRGASGNHVYTKALLAYAFALAGNQDTKKEILKSLDEEAVKEDNSVHWTRAQKPR VPADLWYQPQAPSAEVEMTAYVLLAYLTTELVPTREDLTAAMLIVKWLTKQQNSHGGFSSTQDTVVALHA LSKYGAATFTRAKKAAHVTIQSSGAFYTKFQVNNDNQLLLQRVTLPTVPGDYTAKVAGEGCVYLQTSLKY SVLPREKEFPFALVVQTLPGTCEDLKAHTTFQISLNISYIGSRSDSNMAIADVKMVSGFIPLKPTVKMLE RSVHVSRTEVSNNHVLIYLDKVSNQMLTLFFMVQQDIPVRDLKPAIVKVYDYYEKDEFAVAKYSAPCSAG YGNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175628 |
| ORF Size | 4422 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175628.1, NM_175628.2, NM_175628.3, NP_783327.2 |
| RefSeq Size | 4687 bp |
| RefSeq ORF | 4425 bp |
| Locus ID | 232345 |
| UniProt ID | Q6GQT1 |
| MW | 164.8 kDa |
| Gene Summary | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR212004L3 | Lenti ORF clone of A2m (Myc-DDK-tagged) - Mouse alpha-2-macroglobulin (A2m) |
CNY 14,820.00 |
|
| MR212004L4 | Lenti ORF clone of A2m (mGFP-tagged) - Mouse alpha-2-macroglobulin (A2m) |
CNY 14,820.00 |
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