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Eea1 (NM_001001932) Mouse Tagged ORF Clone
CAT＃: MR211975
- TrueORF®
Eea1 (Myc-DDK-tagged) - Mouse early endosome antigen 1 (Eea1)
ORF Plasmid: tGFP
"NM_001001932" in other vectors (4)
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CNY 9,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A430109M19Rik; B230358H09Rik; ZFYVE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211975 representing NM_001001932
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGAAGGATCTTGCAGAGGACTCCTGGGCGAGTTGGCTCTCAAGGTTCTGATTTAGATTCATCAG CAACTCCTATAAATACGGTGGATGTCAATAATGAAAGTTCTTCAGAGGGTTTCATATGTCCCCAGTGTAT GAAATCCCTTGGCTCTGCTGATGAGCTTTTCAAGCACTATCAGGCCGTTCATGATGCTGGTAATGACTCG GGTCATGGTGGAGAGGCCGGTCTTGCTTTGACACGAGATGACATAACACTACTCAGACAAGAGGTCCAAG ACCTACAGGCCTCACTTAAGGAAGAAAAATGGTACTCAGAAGAATTAAAGAAAGAATTAGAAAAATATCA AGGGCTACAGCAGCAAGAGGCTAAATCTGATGGATTGGTGACTGATTCGTCAGCAGAGCTACAGGCTTTA GAGCAACAATTAGAAGAAGCACAAACAGAGAATTTTAATATTAAGCAAATGAAAGATCTGTTTGAACAGA AAGCAGCCCAACTTGCTACTGAAATTGCAGATATAAAGTCAAAATATGATGAAGAAAAGAGTCTCCGAGC AGCTGCTGAACAAAAAGTGACACATTTGACAGAAGACTTAAACAAACAGACAACTGTAATTCAAGATCTG AAAACTGAACTGCTTCAGAGACCTGGTATAGAAGATGTTGCTGTACTAAAGAAAGAACTGGTCCAAGTTC AAACACTAATGGATAACATGACTTTGGAACGTGAGAGAGAATCTGAAAAACTTAAAGATGAATGCAAAAA GTTACAATCAGAGCATGCTCATTTGGAGGCTACAATAAACCAGCTAAGGAGTGAACTTGCCAAAGGCCCC CAAGAAGTTGCAGTATATGTGCAGGAAATACAAAAACTTAAAGGTTCAATTAATGAATTAACACAAAAAA ACCAGAACTTGACTGAAAAATTGCAGAAGAAAGACCTAGACTACACCCACTTAGAGGAGAAGCACAATGA AGAGTCAGCAAGTCGGAAGACTCTCCAGGCATCCCTCCACCAGCGGGACCTGGATTGCCAGCAGCTTCAG GCAAGGCTAACTGCATCTGAAAGCTCACTCCAGAGGGCCCAGGGAGAACTCAGTGAAAAGGCAGAGGCTG CTCAGAAGCTCAGAGAAGAACTGCGGGAAGTAGAGAGCACGCGCCAGCATCTCAAGGTGGAGGTGAAGCA GCTGCAGCAGCAGCGGGAAGAGAAGGAACAGCACGGGCTGCAGCTGCAGGGGGAAGTCAGCCAGTTACAC TGCAAACTTCTGGAGACCGAGCGCCAACTGGGGGAAGCTCACGGCAGGCTGAAGGAACAGCGGCAGCTTT CCAGCGAGAAGCTGATGGAAAAGGAGCAGCAGGTGGCTGACCTACAGCTCAAGCTCTCTCGTCTGGAGGA GCAGTTGAAGGAAAAGGTAACAAATTCCACTGAATTACAACATCAGTTAGAAAAATCAAAGCAACAGCAT CAAGAACAGCAAGCTCTACAGCAAAGTGCTACAGCCAAACTCCGAGAAGCTCAGAATGATTTGGAGCAAG TGTTACGTCAGATTGGAGATAAGGACCAAAAGATCCAGAACCTTGAAGCCTTGCTGCAGAAGGGTAAAGA AAGCGTTTCCTTACTAGAAAAGGAAAGAGAAGATCTTTATGCAAAAATCCAGGCCGGTGAAGGAGAGACT GCTGTTCTTAACCAGTTACAAGAGAAAAATCATGCACTGCAGCAGCAGCTAACTCAACTGACGGAGAAGC TGAAGAATCAGTCGGAAAGCCATAAACAAGCCGAGGAGAACTTGCATGACCAGGTACAGGAACAGAAGGC ACATCTTCGAGCTGCACAAGACCGTGTTCTTTCTCTGGAAACCAGTGTCAGTGAACTAAGCAGTCAGTTA AATGAAAGCAAGGAGAAGGTCTCGCAGCTTGACATACAGATTAAAGCCAAAACTGAATTATTACTATCAG CAGAAGCAGCAAAAGCTGCTCAGAGAGCTGATCTTCAGAATCACTTGGACACAGCTCAACATGCGTTACA GGATAAACAACAGGAGTTAAATAAGGTCAGTGTGCAGTTGGATCAGCTCACTGCAAAGTTTCAGGAGAAG CAGGAGCATTGCATTCAGCTGGAAAGCCACCTTAAGGATCACAAGGAGAAGCACCTCTCTTTAGAACAAA AAGTTGAAGACTTAGAAGGCCACATTAAGAAACTAGAAGCTGATGCTCTTGAAGTTAAAGCCAGCAAGGA ACAGGCTTTGCAGAGTCTACAGCAGCAGAGGCAGCTGAGCACAGACCTGGAGTTGAGAAATGCAGAGCTG AGTAGAGAGCTCCAGGAGCAGGAGGAAGTAGTATCTTGTACAAAACTGGATCTACAGAATAAATCTGAAA TCCTTGAAAATATCAAACAAACCCTCACCAAGAAAGAAGAAGAAAATGTCGTCTTGAAACAAGAATTTGA AAAGTTAAGTCAAGATTCAAAGACACAGCACAAGGAGCTGGGGGACAGGATGCAAGCTGCGGTCACAGAG CTGACAGCAGTGAAGGCACAGAAGGACGCACTCCTGGCCGAGCTTTCTACAACAAAAGAAAAACTGTCAA AAGTTTCGGATTCTTTAAAAAACTCCAAAAGTGAATTTGAGAAAGAAAATCAGAAAGGAAAAGCTGCTGT ATTAGATTTGGAAAAAGCTTGTAAAGAATTAAAGCATCAGCTTCAAGTACAGGCAGAGAGTGCCCTGAAG GAGCAGGAGGACCTGAAAAAGTCACTCGAGAAAGAGAAAGAGACTTCTCAGCAGTTGAAGATAGAACTCA ATTCAGTCAAGGGAGAAGTCTCACAGGCTCAAAATACATTAAAGCAAAAGGAGAAAGACGAACAACAGCT TCAGGGGACCATAAATCAGCTAAAGCAATCAGCTGAACAAAAGAAAAAACAAATTGAAGCGCTTCAAGGA GAGGTTAAAAATGCTGTTTCACAAAAGACAGTGCTGGAGAATAAGCTGCAGCAGCAGTCATCACAGGCAG CCCAGGAGCTTGCAGCAGAGAAAGGAAAACTGTCAGCGCTGCAGAGCAACTACGAGAAGTGCCAGGCGGA CCTGAAACAGCTGCAGTCCGACCTCTATGGCAAAGAGTCTGAACTTCTAGCCACGAGGCAAGACCTTAAG TCTGTAGAAGAGAAGCTCACTCTGGCACAAGAAGACTTGATTTCAAACAGAAATCAAATTGGAAACCAAA ATAAATCAATTCAAGAATTGCAAGCCGCCAAAGCTAGTTTGGAGCAGGACTCAGCAAAGAAAGAGGCGCT GCTAAAGGAGCAGAGTAAGGCACTTGAGGATGCTCAGAGAGAAAAGTCAGTGAAGGAAAAAGAACTAGTA GCTGAAAAGTCTAAATTGGCAGAGATGGAGGAAATTAAATGTAGACAAGAAAAAGAAATCACAAAACTCA ATGAGGAGCTCAAGTCGCACAAACAAGAAAGCATAAAGGAAATAACAAATCTCAAGGATGCCAAGCAACT TTTGATTCAACAGAAATTAGAACTTCAAGGCAGAGTGGACTCACTGAAGGCAGCTCTTGAGCAGGAGAAG GAGAGTCAGCAGCTGATGCGAGAGCAGGTGAAGAAGGAGGAAGAGAAGCGGAAGGAAGAGTTTTCTGAGA AGGAAGCGAAGCTGCATTCTGAAATAAAAGAAAAGGAAGCGGGAATGAAGAAACACGAAGAAAACGAGGC TAAGCTTACTATGCAGGTCACAACATTAAATGAAAACTTGGGTACTGTGAAGAAGGAGTGGCAGTCTAGT CAACGGAGAGTCAGTGAGCTTGAGAAGCAAACGGATGACTTACGGGGAGAAATTGCTGTCTTAGAAGCAA CTGTTCAGAATAATCAAGACGAAAGAAGAGCACTCCTAGAGAGGTGTCTTAAAGGCGAAGGTGAAATAGA AAAGCTTCAGACCAAAGCCCTAGAGCTGCAGAGAAAGCTGGATAACACAACTGCGGCCGTGCAGGAGCTG GGCAGGGAGAACCAGTCTCTCCAGATCAAACACACACAAGCATTGAACAGAAAGTGGGCGGAAGACAACG AAGTGCAGAACTGCATGTCCTGTGGGAAGTGCTTCTCCGTCACCGTGCGGCGGCATCACTGCCGACAGTG TGGGAATATCTTCTGTGCCGAGTGCTCAACCAAAAATGCCTTAACTCCTTCTTCGAAGAAGCCTGTTCGT GTCTGTGATGCATGCTTCAATGATTTGCAAGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211975 representing NM_001001932
Red=Cloning site Green=Tags(s) MFRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYQAVHDAGNDS GHGGEAGLALTRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKSDGLVTDSSAELQAL EQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEEKSLRAAAEQKVTHLTEDLNKQTTVIQDL KTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSEHAHLEATINQLRSELAKGP QEVAVYVQEIQKLKGSINELTQKNQNLTEKLQKKDLDYTHLEEKHNEESASRKTLQASLHQRDLDCQQLQ ARLTASESSLQRAQGELSEKAEAAQKLREELREVESTRQHLKVEVKQLQQQREEKEQHGLQLQGEVSQLH CKLLETERQLGEAHGRLKEQRQLSSEKLMEKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLEKSKQQH QEQQALQQSATAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKGKESVSLLEKEREDLYAKIQAGEGET AVLNQLQEKNHALQQQLTQLTEKLKNQSESHKQAEENLHDQVQEQKAHLRAAQDRVLSLETSVSELSSQL NESKEKVSQLDIQIKAKTELLLSAEAAKAAQRADLQNHLDTAQHALQDKQQELNKVSVQLDQLTAKFQEK QEHCIQLESHLKDHKEKHLSLEQKVEDLEGHIKKLEADALEVKASKEQALQSLQQQRQLSTDLELRNAEL SRELQEQEEVVSCTKLDLQNKSEILENIKQTLTKKEEENVVLKQEFEKLSQDSKTQHKELGDRMQAAVTE LTAVKAQKDALLAELSTTKEKLSKVSDSLKNSKSEFEKENQKGKAAVLDLEKACKELKHQLQVQAESALK EQEDLKKSLEKEKETSQQLKIELNSVKGEVSQAQNTLKQKEKDEQQLQGTINQLKQSAEQKKKQIEALQG EVKNAVSQKTVLENKLQQQSSQAAQELAAEKGKLSALQSNYEKCQADLKQLQSDLYGKESELLATRQDLK SVEEKLTLAQEDLISNRNQIGNQNKSIQELQAAKASLEQDSAKKEALLKEQSKALEDAQREKSVKEKELV AEKSKLAEMEEIKCRQEKEITKLNEELKSHKQESIKEITNLKDAKQLLIQQKLELQGRVDSLKAALEQEK ESQQLMREQVKKEEEKRKEEFSEKEAKLHSEIKEKEAGMKKHEENEAKLTMQVTTLNENLGTVKKEWQSS QRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKALELQRKLDNTTAAVQEL GRENQSLQIKHTQALNRKWAEDNEVQNCMSCGKCFSVTVRRHHCRQCGNIFCAECSTKNALTPSSKKPVR VCDACFNDLQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001932 |
| ORF Size | 4233 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001932.3, NP_001001932.1 |
| RefSeq Size | 7836 bp |
| RefSeq ORF | 4236 bp |
| Locus ID | 216238 |
| UniProt ID | Q8BL66 |
| MW | 161.4 kDa |
| Gene Summary | Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224411 | Eea1 (untagged) - Mouse early endosome antigen 1 (Eea1), (10ug) |
CNY 18,150.00 |
|
| MG211975 | Eea1 (tGFP-tagged) - Mouse early endosome antigen 1 (Eea1) |
CNY 13,590.00 |
|
| MR211975L3 | Lenti ORF clone of Eea1 (Myc-DDK-tagged) - Mouse early endosome antigen 1 (Eea1) |
CNY 14,250.00 |
|
| MR211975L4 | Lenti ORF clone of Eea1 (mGFP-tagged) - Mouse early endosome antigen 1 (Eea1) |
CNY 14,250.00 |
