Akna (NM_001045514) Mouse Tagged ORF Clone
CAT#: MR211970
- TrueORF®
Akna (Myc-DDK-tagged) - Mouse AT-hook transcription factor (Akna)
ORF Plasmid: tGFP
"NM_001045514" in other vectors (4)
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CNY 9,616.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI597013 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211970 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCAGGGGCAAAGGCACAATGGGTTGGGCCAAGCCTGGGGCAAGGGCCCCGAAGGCGGCGCT GGGCTTGGGCTGAGGAACAAGATACAGATGGAAGAAGAGACCAGGGCTGGGGAAACAGCCAGTCTCTTCC CGAAGCCCCCAGTCCTGAGCTCCTAGAGGACTTCCGTCGGGCACAGGAGCACCTGCCACCCCTGGAGTGG GACCCAGACATGCAGGACTCCGAAGAGTCTTCTGGAGAAGAGACCGAAGCTGACGATGCAAGCAGCCCAG AGGGTTCCACCGTGCCTCTGCCCTGGCTCTCCAGGAGCAACCAACAGCTGGACATGTCTGAAGAGGAGCT GGATGAGGCCTCTGGGAGGCCTGAGGTAGACTTAGCTGGAGAGAGTTGTACGGAGCTTGAGTGTGAAGAC CAGGGAGATTCCAGCCCTCCACCTCCAGGACAGGGGCCAGCCAAAGGCTGGGTCACCTTTATCAAACAAG GCAGTAACTACAGACCTTCAGAGCATCTCGAGGCCCAGCCATCTGTTGAACATAGCCGCACAAAGTCTTG GAGCAGTGGAACTGTGAGCCTCCGTCAACCTAGTGACAGCCTTGGTTCTACCTGGGAAGGAGATACCGAA GTCCCTCAGCCTTCCATCTTGCCCAAAGCCCTGCCACAGAGTCCGTGCCACAACTTTCCACACCCAGGTG ACAGAAATGGAGGTGACGTTGCTCCGGCAACACCCACAGAATTCCGGGACTCCTTAGCAGCGCCAGCCCA GAATGCCGAGTGCTCTGCAGGCACGTGGGGGGAAGAAACCACCAGCCTACCCAGCTCTCGGCCTGAGGAC CAAACCTGGAAGAGGACTAAGACATCACCTAAGCCACTGCCCTCCCGGTTTACTGGTTCTGTCAGCCCCC TGAGTACTCGGCTTGGGGCAATAAAGAAAGTTGTGCCCCAGCACAAGCAGGGAGCCACTCTGGCTGGTCA CTCTTCTTCACAAGCTCCCAAGTATGGCAGGGGGCGCCGCCTGAACTACCCACTCCCCGACTTCTCCAAG GTGGGGCCCCGGGTGAGGTTTCCCAAAGATGAGAACTACCGACCCCCGAAGTCCAGAGGCCACAACAGGC AACAAGGCTCTACCAGACCCCTCATCTTCAAGTCGCCAGCTGAGATTGTTCGGGATGTACTGTTGAGTAG TGGAGAGGCGTCCCTGGCCAAGGAATCTTCTCTTGCTCACACCATCACCAGGGTGCCCCAGGAGTTCCAG ACACCCGAACAAGCCACTGAGCTAGTTCATCAGCTCCAGGAGGACTATCACAAGCTCCTCACCAAGTATG CGGAGGCTGAGAACACTATCGACCAACTTCGCCTTGGGGCCAAGGTGCACCTGTATTCAGACCCACCACA GCCCAGTCAGAGCTTCTGCTCGGGGTCAATGCCACAAGGAAGCAAGGTCTTATCCTTCTCTATCCCGCAA CCCAGAGTAGCAGAGTGGTGGCCTGACCCTGCCCAGGATCCACAGGCCTCTGAGGCCACAGGGTGGCCGT TCCCACGAACAGACCTGAGTCCCTCCTCGTCCCCCGGCGTGGCCACTCCAGGGCGGCTTCCTCAGAGCCA GGGCATTGCCACAGACCAGCCCTCCACAGGACAGACCCAGGCACTGACCTCTCAGGCCAGCCGGCTCCTG GCCAAGGTGCAGTCCTTCGAGGAACTGGTACTGGCTGGACACCTCCCACCTCAGGACCAAATCAAGAGCT TGGAGCAACTGAGGGCAGCCCACATGGCCCTAGAGGCAGAGTACCTGCAGGCCTGCAGAGAAGAGCTCCT GGACCCTCAGCTTGATGCCTCTCAGGGGAGTCCCAGGACCCTCAACCTCTGCAGGGAGCTAGAAGCAGAG ATATACCACCTGGGACAGCGCCTGGAAGAGCTACAGGACCATATGGACCAGACCCAAAGAGAGACAGAGC CATGTAGGCCAGACCTGCAAGACAGCACCCCAACCATGTCCTTTCTGCCCCAGTCAGCTCATCTGTCCAT GCCTTCAGGACCTGTCTCCTTGCCAGATGGCCAAACCTACCAGGAGCCTGCCACCACCACCACCACCTCC CCTGGAAGCTCTTGCACGCTCCCCATCAACAAGAAGTTAAGCCTGAGCATCAAAACTGAGGAAAGCCCAA GAGGTCTCCCGGTCCCACTCAGGGACAGGACGCTTCAGGTGGAGCAGGACTTCCATGGCCTTCTGGAGAG ATACCTCAGCGTTAAATCCCTCCCTGAAGCCTTGAGGGATGAAGATGAGGATGACCTGGAGGAGGAGGAA GAGGAGCAGGACCATCAAGGCCCCTTGGAAGTTGATAGTCCAGCCACAGCTCCAGGGAAAACAGAAGCCG TGAGGGTACCACCAGGAGAGCGCCCAACACAGGCTGAGGAAAGCCATAGGGATGCTACCCAGGAGGATGA GGAACAGATGGGACCTATGAAGTCACCAGACTTCCGGCCATCCATGGCCAGAGATACGTACACACCAGTC CTGGATACAGCTGAAGTGGCTCAACGGGGCACCAAAGCCATGGTGTCACATCAGAGCAGTCTGACCAGCC TGGAAGAGAGCAGGCCCTCAGAGCTCCTTCCTCGAAAAGCCCTGCTTCGGGCTGGTGGGCCCCACACTGA GGAGCCCTGGATGGTGTCACCAGAGACAGACAGTGGCTTCGTGGGCTCAGAAACCAGTATAGTGTCACCC TTCACCCAGACCCCAGAGCACCGGCTCTCCCATGTCAGCACTTCAGGACCATCAGCTCAACACCTCACTG CCTCTGTGCCTGGTGATAGAACCTCCCATCCCAAGGCCAGGGGTCTCATGGTTCCCAGAAGAGCCACTGA GACTGGCATACCCAGAAGCCGAACCCAGCAGCATTTCTCCAGCCTGAGCTCTCCCGGGAGAGGGGCACAG AGCTGCCACCTAGAGGAGACATCAGTGGCTAAAATAGCGGTTCCCAGGTCTGAGTTCAAGAGACAGAAGC AGATTTCCAAACAGCTCCTTCCCAGTGGAAGAACCAGCCCCGACTCTGCCCCAGCTCCCACAGCTGCCTC CACGCCCCATGGATCAGCAGAGAGCACTGCCAACCTCCTCCTCAACAGGACAGAGCGCGACCAGGCCATC AAGGATCTGCAGGCGGAGGTGTCACGGCTCCGCCTTCAACTGGAGGACAGCCTGCACCGGCCACACCCAG ACGGCCCAGCCTGTGTAGCTTCTGCTTTCAACCACTCCACCCAGACCCAGGAAAAGCTGGGTTCCTCACC CTCCTGGGGCCCCCACTATGGCAGTAAATCCACAGAGAGGCTGTCTAGGGAGCCTAATGGTGTAGAGCCA GCTGAGCCAATGGGAAGACGTCGAGCCAGGTCCTCTTCGGTGCCTCGGGATGTACCCAGACTGTACCTAA GTTCTGAATCGGAGTCGCCTGCCCCTCGGCTCTCATCTGAGAAGAGCAGGACCTTTGAGGAGCACCCCGA GGCTGCACAGTGGGGGACAAGACCACAGAGCAGCTCGAAGCGGAGGGAAAGAGTTTCCTTCCGGGGTCAA TACACAGGCCAGGAGTACCACATTCTATCCCCAAAGGCCATCCTGAAGGACAGTGGCACACCGTCCTGTC CCCACTGCCATCCCATTAGGACCCAGGACACAGGCAGTGCTGTCTCTAGAGACACCACACGGGGCTCATC TGCCGCTGACACCCTCCGCTGTGCCCTGTGTGGTGAAGTCAAGTCCTCTGCAGAAGCAGATGGCTCCAGC TCAGGTCCCTCTGAGAAGAACACCCCCAAAAAGCCTTCCACCCCCATCCTCAAACGGAAGAACAGGCAGA CGGGCTCGCCAGTACGAATGGCCCCTGGACTCTGGTACCTGGCTGCTGCGCCCCCAGCCCCAGCCCCACC GGCTTTGGCCTACATCTCCTCAGCTCCCATTATGCCTTATCTACCACCCACTGTGTACTATGCAGCCCCA GCACCTACCTCAGCCCAGACAGCTTCCCCGCAGCCTGCTCGGGGACCTCGGAGAACCCGGCACTCAGTCC AACTGGGTCTGAATGACCTGGAGGAGCTGCAGGCCGCACTCAGAGAGGCTGCACAGGCCGCCGAGAACGT TCGCTCCACCACCCGCCAGCTGAGCCGCTCCCTGTCTGCGGACCTGCGCCATGCCCGTAGCCTGCGAGGC TCCTGCCTTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211970 protein sequence
Red=Cloning site Green=Tags(s) MASSGAKAQWVGPSLGQGPRRRRWAWAEEQDTDGRRDQGWGNSQSLPEAPSPELLEDFRRAQEHLPPLEW DPDMQDSEESSGEETEADDASSPEGSTVPLPWLSRSNQQLDMSEEELDEASGRPEVDLAGESCTELECED QGDSSPPPPGQGPAKGWVTFIKQGSNYRPSEHLEAQPSVEHSRTKSWSSGTVSLRQPSDSLGSTWEGDTE VPQPSILPKALPQSPCHNFPHPGDRNGGDVAPATPTEFRDSLAAPAQNAECSAGTWGEETTSLPSSRPED QTWKRTKTSPKPLPSRFTGSVSPLSTRLGAIKKVVPQHKQGATLAGHSSSQAPKYGRGRRLNYPLPDFSK VGPRVRFPKDENYRPPKSRGHNRQQGSTRPLIFKSPAEIVRDVLLSSGEASLAKESSLAHTITRVPQEFQ TPEQATELVHQLQEDYHKLLTKYAEAENTIDQLRLGAKVHLYSDPPQPSQSFCSGSMPQGSKVLSFSIPQ PRVAEWWPDPAQDPQASEATGWPFPRTDLSPSSSPGVATPGRLPQSQGIATDQPSTGQTQALTSQASRLL AKVQSFEELVLAGHLPPQDQIKSLEQLRAAHMALEAEYLQACREELLDPQLDASQGSPRTLNLCRELEAE IYHLGQRLEELQDHMDQTQRETEPCRPDLQDSTPTMSFLPQSAHLSMPSGPVSLPDGQTYQEPATTTTTS PGSSCTLPINKKLSLSIKTEESPRGLPVPLRDRTLQVEQDFHGLLERYLSVKSLPEALRDEDEDDLEEEE EEQDHQGPLEVDSPATAPGKTEAVRVPPGERPTQAEESHRDATQEDEEQMGPMKSPDFRPSMARDTYTPV LDTAEVAQRGTKAMVSHQSSLTSLEESRPSELLPRKALLRAGGPHTEEPWMVSPETDSGFVGSETSIVSP FTQTPEHRLSHVSTSGPSAQHLTASVPGDRTSHPKARGLMVPRRATETGIPRSRTQQHFSSLSSPGRGAQ SCHLEETSVAKIAVPRSEFKRQKQISKQLLPSGRTSPDSAPAPTAASTPHGSAESTANLLLNRTERDQAI KDLQAEVSRLRLQLEDSLHRPHPDGPACVASAFNHSTQTQEKLGSSPSWGPHYGSKSTERLSREPNGVEP AEPMGRRRARSSSVPRDVPRLYLSSESESPAPRLSSEKSRTFEEHPEAAQWGTRPQSSSKRRERVSFRGQ YTGQEYHILSPKAILKDSGTPSCPHCHPIRTQDTGSAVSRDTTRGSSAADTLRCALCGEVKSSAEADGSS SGPSEKNTPKKPSTPILKRKNRQTGSPVRMAPGLWYLAAAPPAPAPPALAYISSAPIMPYLPPTVYYAAP APTSAQTASPQPARGPRRTRHSVQLGLNDLEELQAALREAAQAAENVRSTTRQLSRSLSADLRHARSLRG SCLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001045514 |
ORF Size | 4215 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001045514.3, NP_001038979.1 |
RefSeq Size | 5513 bp |
RefSeq ORF | 4215 bp |
Locus ID | 100182 |
UniProt ID | Q80VW7 |
MW | 153.1 kDa |
Gene Summary | Centrosomal protein that plays a key role in cell delamination by regulating microtubule organization (PubMed:30787442). Required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis (PubMed:30787442). Also regulates the epithelial-to-mesenchymal transition in other epithelial cells (PubMed:30787442). Acts by increasing centrosomal microtubule nucleation and recruiting nucleation factors and minus-end stabilizers, thereby destabilizing microtubules at the adherens junctions and mediating constriction of the apical endfoot (PubMed:30787442). In addition, may also act as a transcription factor that specifically activates the expression of the CD40 receptor and its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for antigen-dependent-B-cell development (By similarity). Binds to A/T-rich promoters (By similarity). It is unclear how it can both act as a microtubule organizer and as a transcription factor; additional evidences are required to reconcile these two apparently contradictory functions (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224410 | Akna (untagged) - Mouse AT-hook transcription factor (Akna), (10ug) |
CNY 18,150.00 |
|
MG211970 | Akna (tGFP-tagged) - Mouse AT-hook transcription factor (Akna) |
CNY 11,880.00 |
|
MR211970L3 | Lenti ORF clone of Akna (Myc-DDK-tagged) - Mouse AT-hook transcription factor (Akna) |
CNY 12,730.00 |
|
MR211970L4 | Lenti ORF clone of Akna (mGFP-tagged) - Mouse AT-hook transcription factor (Akna) |
CNY 12,730.00 |