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Nid2 (NM_008695) Mouse Tagged ORF Clone
CAT＃: MR211968
- TrueORF®
Nid2 (Myc-DDK-tagged) - Mouse nidogen 2 (Nid2)
"NM_008695" in other vectors (3)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW547149; entactin-2; Ly111; NID-2; nidogen-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211968 representing NM_008695
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGGGACCCGACAGCCGGGTGGCTAACACCGCCATCACCGCTATCGCTGCTGGTGATGCTGTTGC TGCTGTCGCGAGTCGGGGCTCTGCGTCCCGACGAGCTCTTTCCTTACGGGGAGTCGTGGGGAGACCAGCT GCTGCCGGAGGGCGACGACGAAAGCTCAGCAGCTGTGAAGCTGGCAATACCCCTGCGTTTCTACGATGCC CAGTTCAGCAGCCTCTACGTGGGCACCAACGGCATCATCTCCACCCAGGACTTCCCCAGGGAGACTCAAT ATGTAGATGATGATTTTCCTACTGACTTCCCAGCCATCGCCCCTTTCCTGGCTGACATCGACACTAGTCA CAGCAGGGGCCGGATCCTGTACCGCGAGGACACCTCCGGAGCTGTGCTGAGCCTGGCTGCCCGCTACGTG CGCACTGGCTTCCCGCTGTCGGGGTCCAGCTTCACCCCCACCCACGCCTTCCTGGCCACCTGGGAGCACG TGGGCGCCTACGAGGAGGTCAGTCGCGGGGCTGCGCCGTCAGGAGAGCTGAACACTTTCCAGGCTGTTCT GGCATCCGATGAATCTGATACCTACGCCCTCTTTCTCTACCCTGCCAATGGCCTTCAGTTCTTTGGAACC CGCCCCAAAGAGTCCTACAATGTCCAGCTGCAGCTTCCCGCTAGGGTGGGCTTCTGCCGAGGGGAAGCGG ACGACCTGAAGAGAGAAGCACTGTATTTCAGCCTGACTAACACCGAGCAGTCTGTGAAGAATCTCTACCA GCTAAGCAACCTGGGGATTCCTGGAGTGTGGGCTTTTCACATCGGCAGCAGGTTTGCCCTGGACAATGTC CGTCCAGCCACAGTTGGAGGTGACCCTTCCACAGCCCGTTCCTCAGCACTGGAGCACCCCTTCAGCCATG CTGCAGCCCTGGAGAGCTACACCGAGGACAGTTTCCATTACTACGATGAGAATGAAGAGGATGTTGAGTA CCCACCAGTTGAACCGGGGGAGGCCCCGGAAGGCCACAGCAGAATTGATGTGTCTTTCAATTCAAAGGCT GATCCAGGCCTTGTGGACGTCGGCACCTCATCTCCAGGTTCCGACCGCGCTTCTCCTTGGCCATATCCAG CACCTGGTAACTGGCCATCCTACCGGGAAACAGAATCGGCTTCTTTGGACCCTCAAACCAAACAGGGGCG GCCGGTGGGAGAGGGAGAGGTTCTGGATTTCAGGGACCCAGCGGAGCTTTTGGACCAGATGGGTACCAGA GCCCCAGCTCCTCCAGAGGCAGATGCAGCTCTCCTAACTCCAGTCAACGAAGACCTTGGGGGCAGAAACA CCCAGTCCTATCCAGAGGCAGGGCCAGTGCCTTCAGAGCCAGATGTTCCTGTCCCTCCTCTGGAAGGAGA AGTCCTTCCTCATTACCCCGAGTCTGGTCACGTTCCGCCCCTGAGAGGAGGGAAGTATGTGATAGGACTG GAGGACCACGTGGGATCTAATGATCAAGTCTTCACGTATAATGGCGCCAACCTGGAAACCTGCGAACACA GCCACGGCCGGTGCTCCCAGCATGCCTTCTGCACCGACTACACCACTGGCTTTTGCTGCCACTGCCAGTC GAGGTTTTACGGAAACGGGAAGCACTGCCTGCCAGAAGGGGCACCTCACCGAGTGAATGGGAAAGTGAGT GGTCGCCTCCGAGTGGGTCACATTCCTGTGCACTTCACTGACGTGGATCTACACGCTTACATCGTGGGCA ATGATGGCAGAGCCTACACTGCCATCAGCCATGTCCCACAGCCGGCAGCCCAGGCCCTGCTCCCTGTCCT GCCCATTGGAGGTCTGTTTGGCTGGCTCTTTGCTTTGGAGAAGCCAGGCTCTGAGAATGGCTTCAGCCTC ACAGGTGCTACCTTTGTCCATGACGTGGAAGTCACTTTTCACCCTGGAGAGGAGAGGGTTCGTATCACTC AAACTGCCGAGGGGCTTGACCCAGAAAACTATCTGAGCATTAAGACCAACATTGAAGGCCAGGTGCCCTT CATCCCAGCGAATTTCACAGCCCACATTACTCCGTACAAAGAGTTTTATCATTACAGGGACTCAGTCGTG ACGTCCTCCAGTTCCCGAAGTTTCTCTCTCACTTCTGGTTCTATTAACCAAACGTGGTCCTACCACATTG ACCAGAACATCACTTACCAGGCGTGCAGGCACGCCCCCAGACACCTGGCCATCCCTGCCACCCAGCAGCT GACTGTCGACCGGGCCTTTGCTTTGTACAGTGAGGATGAGGGCGTGCTGAGGTTTGCTGTGACCAATCAG ATTGGCCCTGTTGAAGTGGACTCAGCCCCTGTTGGAGTGAATCCTTGCTACGACGGAAGCCACACCTGTG ACACAACAGCAAGGTGCCACCCGGGAACAGGTGTAGACTACACCTGCGAGTGCACGCCTGGGTTCCAGGG TGACGGACGGAGCTGTGTGGATGTCAATGAGTGTGCCACTGGCTTCCACCGCTGTGGCCCTAATTCTGTG TGTGTCAACTTGGTGGGAAGCTACAGGTGTGAATGCCGCAGTGGCTATGAATTTGCAGATGACCAGCACA CTTGTATCTTGATCGCCCCACCTCCCAACCCTTGCCTCGATGGCAGTCACACCTGTGCCCCTGAGGGGCA AGCCAGGTGCATTCACCATGGAGGCAGCTCATTCAGTTGTGCCTGCCTGCCAGGCTTCATTGGCACTGGG CATCAGTGTTCTGATGTTGATGAATGTGCGGAAAACCGATGTCATGAGGCAGCTATCTGCTACAATACCC CCGGGTCCTTCTCCTGCCGTTGCCAGCCTGGGTATCGAGGGGACGGGTTTCACTGCACCTCTGACACGGT TCCGGAAGATTCCATCTCAGGACTGAAGCCCTGTGAATACCAGCAGCGCTATGCTCAGACACAGCACGCC TACCCTGGGTCACGGATCCACATCCCCCAGTGTGATGACCAGGGAAACTTCGTGCCACTGCAGTGCCACG GCAGCACTGGCTTCTGCTGGTGTGTGGACCGAAATGGCCACGAAGTCCCTGGCACGCAGACTCCACCCGG CTCCACCCCGCCCCACTGTGGACCACCTCCAGAGCCCACCCAGAGGCCTCGGACTGTCTGTGAGCGTTGG CGGGAAAGCCTGCTGGAACACTACGGAGGCACACCCAGGGATGACCAGTATGTGCCCCAGTGTGATGACC TGGGCCACTTCATCCCCCTGCAGTGCCATGGAAAGAGTGATTTCTGTTGGTGTGTGGACAAGGATGGCAG AGAGCTACAGGGCACGCGCTCACAGCCAGGCACCAGGCCTGCATGCATACCCACCGTCGCTCCACCCGTG GTCCGGCCCACACCCCGGCCCGATGTGACTCCTCCATCTGTGGGCACCTTCCTGCTCTATGCCCAGGGCC AACAGATTGGCCACTTGCCCCTCAATGGCAGCAGGCTTCAGAAGGACGCAGCCCGGACCCTGCTGTCACT GCATGGCTCCATAGTTGTGGGGATTGACTATGACTGCCGGGAGAGGATGGTCTACTGGACAGACGTTGCT GGTCGGACCATCAGCCGTGCCAGCTTGGAGGCAGGAGCCGAACCCGAGACCATCATTACCTCAGGTTTGA TAAGCCCGGAAGGACTTGCCATCGACCACTTCCGTAGAACAATGTACTGGACAGATAGTGGCCTGGATAA GATAGAGCGGGCCGAACTGGATGGTTCCGAGCGGAAAGTCCTCTTCCACACAGATCTGGTGAATCCACGA GCCATCACTGTGGATCCAATCCGAGGCAACTTGTACTGGACAGACTGGAATAGAGAAGCTCCTAAAATTG AAACATCATCTTTAGATGGCGAAAACAGAAGGATTCTGATCAACAAAGATATTGGATTACCCAATGGATT GACCTTTGACCCCTTCTCCAAACTGCTCTGCTGGGCAGATGCAGGAACCAAAAAACTGGAGTGTACACTA CCCGATGGAACTGGACGACGTGTCATCCAAAACCACCTCAATTACCCCTTCAGCATCGTCAGCTATGCAG ATCACTTCTACCACACGGACTGGCGGAGGGATGGTGTTATATCAGTGAATAAGGACAGTGGCCAGTTTAC TGATGAGTTTCTCCCTGAGCAGCGGTCTCATCTCTATGGGATCACTGCAGTCTACCCGTACTGTCCAACA GGAAGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211968 representing NM_008695
Red=Cloning site Green=Tags(s) MFRDPTAGWLTPPSPLSLLVMLLLLSRVGALRPDELFPYGESWGDQLLPEGDDESSAAVKLAIPLRFYDA QFSSLYVGTNGIISTQDFPRETQYVDDDFPTDFPAIAPFLADIDTSHSRGRILYREDTSGAVLSLAARYV RTGFPLSGSSFTPTHAFLATWEHVGAYEEVSRGAAPSGELNTFQAVLASDESDTYALFLYPANGLQFFGT RPKESYNVQLQLPARVGFCRGEADDLKREALYFSLTNTEQSVKNLYQLSNLGIPGVWAFHIGSRFALDNV RPATVGGDPSTARSSALEHPFSHAAALESYTEDSFHYYDENEEDVEYPPVEPGEAPEGHSRIDVSFNSKA DPGLVDVGTSSPGSDRASPWPYPAPGNWPSYRETESASLDPQTKQGRPVGEGEVLDFRDPAELLDQMGTR APAPPEADAALLTPVNEDLGGRNTQSYPEAGPVPSEPDVPVPPLEGEVLPHYPESGHVPPLRGGKYVIGL EDHVGSNDQVFTYNGANLETCEHSHGRCSQHAFCTDYTTGFCCHCQSRFYGNGKHCLPEGAPHRVNGKVS GRLRVGHIPVHFTDVDLHAYIVGNDGRAYTAISHVPQPAAQALLPVLPIGGLFGWLFALEKPGSENGFSL TGATFVHDVEVTFHPGEERVRITQTAEGLDPENYLSIKTNIEGQVPFIPANFTAHITPYKEFYHYRDSVV TSSSSRSFSLTSGSINQTWSYHIDQNITYQACRHAPRHLAIPATQQLTVDRAFALYSEDEGVLRFAVTNQ IGPVEVDSAPVGVNPCYDGSHTCDTTARCHPGTGVDYTCECTPGFQGDGRSCVDVNECATGFHRCGPNSV CVNLVGSYRCECRSGYEFADDQHTCILIAPPPNPCLDGSHTCAPEGQARCIHHGGSSFSCACLPGFIGTG HQCSDVDECAENRCHEAAICYNTPGSFSCRCQPGYRGDGFHCTSDTVPEDSISGLKPCEYQQRYAQTQHA YPGSRIHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERW RESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELQGTRSQPGTRPACIPTVAPPV VRPTPRPDVTPPSVGTFLLYAQGQQIGHLPLNGSRLQKDAARTLLSLHGSIVVGIDYDCRERMVYWTDVA GRTISRASLEAGAEPETIITSGLISPEGLAIDHFRRTMYWTDSGLDKIERAELDGSERKVLFHTDLVNPR AITVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINKDIGLPNGLTFDPFSKLLCWADAGTKKLECTL PDGTGRRVIQNHLNYPFSIVSYADHFYHTDWRRDGVISVNKDSGQFTDEFLPEQRSHLYGITAVYPYCPT GRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008695 |
| ORF Size | 4209 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008695.2, NP_032721.2 |
| RefSeq Size | 4913 bp |
| RefSeq ORF | 4212 bp |
| Locus ID | 18074 |
| UniProt ID | O88322 |
| MW | 154.4 kDa |
| Gene Summary | Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202583 | Nid2 (untagged) - Mouse nidogen 2 (Nid2), (10ug) |
CNY 9,608.00 |
|
| MR211968L3 | Lenti ORF clone of Nid2 (Myc-DDK-tagged) - Mouse nidogen 2 (Nid2) |
CNY 12,730.00 |
|
| MR211968L4 | Lenti ORF clone of Nid2 (mGFP-tagged) - Mouse nidogen 2 (Nid2) |
CNY 12,730.00 |
