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Edc4 (NM_181594) Mouse Tagged ORF Clone
CAT＃: MR211958
- TrueORF®
Edc4 (Myc-DDK-tagged) - Mouse enhancer of mRNA decapping 4 (Edc4)
ORF Plasmid: tGFP
"NM_181594" in other vectors (4)
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CNY 9,512.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC022641 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211958 representing NM_181594
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCTGCGCGAGCATCGACATTGAGGACGCCACGCAGCATCTGCGGGACATACTCAAACTGGACC GGCCCGCCGGAGGTTCCAATGCCGAAAGCCAAAGGCCATCCAGTGCCTACAATGGAGACCTCAATGGGCT CCTGGTCCCCGACCCGCTCAGTTCAGGTGATGGTAACTCAACGAACAAACCTGGTATACGGACCATGCCA CCCATTAACCTGCAGGAAAAGCAGGTCATTTGCCTCTCTGGAGATGACAGCTCCACCTGCATTGGGATTT TAGCCAAGGAAGTGGAAATTGTGGCCAGCAGTGATTCTAGCATTTCCAGCAAGGCTCGGGGGAGCAACAA GGTGAAAATCCAGCCTGTCGCCAAGTATGACTGGGAGCAGAAATACTACTATGGCAACCTGATTGCAGTG TCTAACTCCTTCTTGGCATACGCCATTAGGGCTGCCAACAATGGCTCAGCAATGGTCCGTGTGATCAGTG TCAGCACTTCGGAGCGGACCCTGCTCAAAGGTTTCACAGGCAGTGTGGCTGATCTTGCTTTTGCACACCT CAACTCTCCACAGCTCGCCTGCCTGGATGAGGCTGGCAATCTTTTTGTGTGGCGCTTGGCTCTTGTTAAG GGTAAAATTCAAGAAGAGATTTTAGTCCATATCCGGCAGCCAGAGGGTACAGCGCTGAACCACTTCCGAA GGATCATCTGGTGCCCTTTCATCCCCGAGGAGAGTGAGGACTGTTGTGAGGAGAGCAGCCCGACAGTGGC CCTGCTGCATGAGGATCGGGCTGAGGTGTGGGACTTGGATATGCTTCGCTCCAGCCACAACACCTGGCCT GTGGATGTCAGCCAAATCAAGCAAGGCTTTATTGTGGTGAAAGGCCATAGCACGTGCCTAAGTGAAGGAG CCCTCTCCCCTGATGGGACTGTCCTGGCTACCGCAAGCCATGATGGCTTTGTCAAGTTCTGGCAGATCTA TATTGAAGGTCAGGATGAGCCAAGGTGTCTGCATGAATGGAAGCCTCATGATGGGAGGCCTCTTTCTTGC CTCCTGTTCTGTGATAACCATAAGAAACAGGATCCTGAGGTCCCGTTCTGGAGGTTCCTCATAACCGGCG CCGACCAGAATCGAGAGCTGAAGATGTGGTGCACAGTGTCCTGGACCTGCCTGCAAACCATTCGCTTCTC CCCAGATATCTTCAGCTCAGTGAGTGTGCCTCCCAGCCTCAAAGTTTGTTTGGACCTCTCAGCAGAGTAT TTGATTCTTAGTGATGTGCAACGAAAGGTCCTCTATGTGATGGAGCTGCTGCAGAACCAGGACGAGGGCC GCGCGTGCTTTAGTTCTATCTCTGAGTTCCTGCTCACCCACCCTGTGCTGAGCTTTGGCATCCAGGTTGT CAGTCGCTGCCGGCTACGGCACACTGAGGTCCTGCCTGCTGAGGAGGAGAATGACAGTCTGGGGACCGAG AGTTCCCATGGAGCTGGAGCCTTGGAATCCGCAGCTGGTGTGCTTATCAAGCTCTTTTGTGTGCACACTA AGGCACTACAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCTGATGTGGTGGCCCCACTCTCTAC CCACACTGCCCACGAGGACTTCACATTTGGAGAGTCTCGACCTGAACTAGGCTCCGAGGGGCTAGCTTCA GCTGCTCATGGTTCCCAGCCTGACCTGCGGCGCATTGTGGAACTGCCTGCTCCTGCAGACTTCCTCAGTC TGAGCAGTGAGACCAAGCCCAAGTTGATGACACCTGATGCTTTCATGACACCTACTGCCTCCCTGCAGCA GATCTCTGCATCCCCTAGTAGTAGCAGCAGCAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGC AGCTCTCTAACAGCTGTGTCTGCTGTGAGCAGCTCCTCAGCCATGGACCCTTCCTTGCCCAGACCACCTG AGGAGCTGACCTTGAGCCCCAAGCTGCAGCTAGATGGCAGCCTGACGCTAAACAGCAGCAGCAGCAGCCT GCAGGCAAGCCCTCGAAGCCTCCTTCCGGGCCTGCTCCCAGGCCCAGCTGACAAGTTGATTTCCAAGGGA CCTGGTCAGGTATCCACTGCTGCCTCTGCACTGTCCTTGGATTTGCAGGAAGTGGAGCCATTAGGGCTAC CCCAGGCCTCCCCAAGTCGAACCCGTTCTCCTGATGTGATCTCCTCAGCATCCACTGCCCTGTCCCAGGA CATTCCTGAAATCGCATCTGAGGCCCTGTCCCGTGGCTTTGGCTCCTCTGTTCCTGAGGGCCTCATTGAG CCAGACAGCATGGCCTCAGCTGCCTCAGCACTACACCTACTCTCTCCTCGGCCCAGGCAAGGCCCTGAGC TTGGCTCTCAGCTGGGTCTGGATGGAGGCCCTGGAGATGGGGATAGGCATAGTACCCCTTCCCTGCTGGA AGCAGCCCTGACCCAGGAAGTTGCAACCCCTGACAGTCAAGTCTGGCCTACGGCACCTGACATTACTCGT GAGACCTGTAGCACACTGACAGAAAGGCCACCTTATCACTTGCTGCAGCAACGTGACAGTCAGGACACAA GTGCTGAGCAAAGTGACCATGATGATGAGGTTGCCAGCCTTGCCTCTGCCTCAGGAGGTTTTGGCAGCAA AATTCCTACTCCACGGCTGCCTTCCAAGGATTGGAAGACGAAGGGATCCCCTCGGACGTCACCTAAGCTC AAGAGAAAAAGCAAGAAGGATGATGGGGATTCAGCTGTGGGATCCCGGCTCACAGAGCACCAGGTGGCAG AGCCCCCTGAAGACTGGCCAGCACTAATTTGGCAGCAGCAAAGAGAGCTGGCAGAGCTATGGCACAACCA AGAAGAACTGCTACAGCGTCTCTGTGCCCAGCTTGAAGGTCTTCAGAGTACTGTCACAGACCATGTAGAG CGTGCCCTGGAGACGCGGCATGAGCAGGAGCAACGGCGGCTGGAACGGGCACTGGCTGAGGGGCAGCAAC GGGGTGGGCAGCTGCAGGAGCAGCTGACACAGCAGCTATCCCAGGCATTGTCTTCAGCTGTGGCTGGGCG GCTAGAGCGCAGCGTAAGGGATGAAATCAAGAAAACGGTTCCTCCATGTGTCTCCAGAAGTCTGGAGCCT GTTGCAGGTCAACTAAGCAACTCAGTGGCTACCAAGCTTACAGCTGTGGAAGGCAGCATGAAAGAGAATA TCTCTAAGCTACTCAAGTCCAAGAACTTAACAGATGCCATTGCCCGAGCAGCTGCAGACACGTTACAGGG ACCAATGCAGGCTGCCTACCGGGAAGCCTTCCAGAGTGTGGTACTGCCAGCTTTTGAGAAGAGTTGCCAG GCTATGTTCCAGCAAATCAATGACAGCTTTAGGCTGGGCACACAGGAATATTTGCAGCAGCTGGAGAGTC ACATGAAGAGCCGGAAGGCCCGTGAACAAGAAGCTAGGGAGCCTGTGCTGGCCCAGCTTCGGGGCCTGGT CAGCACACTGCAGAGTGCCACCGAGCAGATGGCGGCCACTGTATCTAGCAGTGTTCGGGCCGAGGTGCAA CACCAGCTGCATGTGGCTGTGGGCAGCTTGCAGGAGTCAATCTTAGCACAAGTACAGCGCATTGTCAAGG GTGAAGTGAGTGTGGCACTTAAGGAGCAGCAGGCCACCGTCACTTCCAGCATCATGCAGGCTATGCGTTC AGCTGCTGGCACACCTGTCCCCTCTGCCCACCTTGACTGCCAGGCCCAACAAGCCCATATCTTGCAGTTA CTGCAGCAGGGCCACCTCAATCAGGCCTTCCAGCAGGCACTGACTGCTGCTGATCTCAATCTGGTGCTGT ATGTGTGTGAAACTGTCGACCCAGCCCAGGTTTTTGGGCAGCCGCCCTGTCCACTATCCCAGCCTGTTCT CCTTTCCCTAATCCAGCAGCTAGCATCTGACCTTGGAACTAGAAGTGACCTCAAGCTCAGCTACTTGGAA GAAGCTGTGATGCACCTGGACCACAGCGATCCCATCACTCGAGACCACATGGGCTCTGTCATGGCTCAGG TGCGCCAGAAGCTCTTCCAGTTCCTGCAGGCTGATCCACACAACTCACTTAGCAAAGCTGCCCGCCGCCT CAGCCTAATGTTACACGGCCTTGTGACCCCTAGCCTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211958 representing NM_181594
Red=Cloning site Green=Tags(s) MASCASIDIEDATQHLRDILKLDRPAGGSNAESQRPSSAYNGDLNGLLVPDPLSSGDGNSTNKPGIRTMP PINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAV SNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVK GKIQEEILVHIRQPEGTALNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHNTWP VDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGFVKFWQIYIEGQDEPRCLHEWKPHDGRPLSC LLFCDNHKKQDPEVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEY LILSDVQRKVLYVMELLQNQDEGRACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGTE SSHGAGALESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLSTHTAHEDFTFGESRPELGSEGLAS AAHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPTASLQQISASPSSSSSSSSSSSSSSSSSS SSLTAVSAVSSSSAMDPSLPRPPEELTLSPKLQLDGSLTLNSSSSSLQASPRSLLPGLLPGPADKLISKG PGQVSTAASALSLDLQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSVPEGLIE PDSMASAASALHLLSPRPRQGPELGSQLGLDGGPGDGDRHSTPSLLEAALTQEVATPDSQVWPTAPDITR ETCSTLTERPPYHLLQQRDSQDTSAEQSDHDDEVASLASASGGFGSKIPTPRLPSKDWKTKGSPRTSPKL KRKSKKDDGDSAVGSRLTEHQVAEPPEDWPALIWQQQRELAELWHNQEELLQRLCAQLEGLQSTVTDHVE RALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSVRDEIKKTVPPCVSRSLEP VAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYREAFQSVVLPAFEKSCQ AMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSATEQMAATVSSSVRAEVQ HQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQATVTSSIMQAMRSAAGTPVPSAHLDCQAQQAHILQL LQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQLASDLGTRSDLKLSYLE EAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQADPHNSLSKAARRLSLMLHGLVTPSLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181594 |
| ORF Size | 4170 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181594.3, NP_853625.1 |
| RefSeq Size | 4755 bp |
| RefSeq ORF | 4173 bp |
| Locus ID | 234699 |
| UniProt ID | Q3UJB9 |
| MW | 151.1 kDa |
| Gene Summary | The protein encoded by this gene is thought to promote mRNA decay, and is known to interact with several mRNA decapping proteins. In humans, decreased expression of this gene prevents the accumulation of mRNA decapping proteins to mRNA processing bodies (P-body). Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203631 | Edc4 (untagged) - Mouse enhancer of mRNA decapping 4 (Edc4), (10ug) |
CNY 9,520.00 |
|
| MG211958 | Edc4 (tGFP-tagged) - Mouse enhancer of mRNA decapping 4 (Edc4) |
CNY 13,490.00 |
|
| MR211958L3 | Lenti ORF clone of Edc4 (Myc-DDK-tagged) - Mouse enhancer of mRNA decapping 4 (Edc4) |
CNY 14,160.00 |
|
| MR211958L4 | Lenti ORF clone of Edc4 (mGFP-tagged) - Mouse enhancer of mRNA decapping 4 (Edc4) |
CNY 14,160.00 |
