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Usp19 (NM_027804) Mouse Tagged ORF Clone
CAT＃: MR211946
- TrueORF®
Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 1
ORF Plasmid: tGFP
"NM_027804" in other vectors (4)
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CNY 9,304.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 8430421I07Rik; AI047774; Zmynd9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211946 representing NM_027804
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCAGGGGCCAGTGCTACAGGGCCCAGGAGGGGGCCGCCAGGACTGGAAGAGGCCACTAGTAAGA AGAAACAGAAGGATCGAGCAAACCTGGAAAGTAAAGATGGAGATGCTAGGAGAGTGTCCCTTCCTCGAAA GGAACCAACCAAAGATGAATTGTTGCTCGATTGGAGGCAGAGTGCAGATGAGGTGATTGTTAAGCTGCGC GTGGGAACAGGTCCCGTACGTCTGGAGGATGTAGATGCTGCGTTCACAGACACGGACTGTGTGGTGAGGC TTCCAGATGGTCGGCAGTGGGGTGGTGTCTTCTTTGCTGAAATACAAAGTTCTTGCACCAAAGTGCAGGC TCGCAAGGGTGGTCTTCTACAGCTAGTACTACCCAAGAAGGTGCCTCTGCTCACGTGGCCCTCTCTCCTG AAACCTCTGGGAACCCAAGAGCTGGTGCCAGGTTTGCAGTGCCAGGAGAACGGGCAAGAGCTGTCTCCCA TTGCCCTGGAGCCAGGCTCTGAGCCCCGCAGAGCTAAACAGGAAGCCCGAAACCAGAAGCGGGCCCAGGG CCGTGGTGAGGTAGGCTCGGGGGCTGGCCCTGGGACACAGGCAGGGCCCAGCGCCAAGAGGGCTGTTCAC CTCCGCAGAGGGCCAGAAGGGGAAGGGTCCATGGATGGCCCCGGCCCCCAGGGTGATGCCCCGTCTTTCC TGTCTGACTCAGCTACCCAGGTTGAGGCTGAGGAGAAGCTCTGTGCTCCACCAATGAACACTCAAACAAG TCTCTTGAGCTCAGAGAAGAGTTTAGCCCTTCTGACAGTAGAGAAGACAGTGTCCCCCAGGAATGACCCA GTCGCCCCGGTTATGGTCCAGGACAGAGACCCTGAGCCTGAGCAAGAAGACCAAGTCAAAGAGGAGATGG CACTTGGGGCTGATCCTACAGCCTTGGTGGAGGAACCAGAGTCTATGGTGAACCTGGCATTTGTCAAGAA CGACTCGTATGAGAAGGGCCCGGATTCGGTGGTGGTGCACGTGTACGTGAAGGAGATCCGCAGGGACAGC TCCCGAGTGCTCTTCCGAGAGCAGGACTTCACACTGATCTTCCAGACCAGGGACGGAAACTTTCTGAGGC TGCATCCGGGCTGTGGGCCCCACACCATCTTCCGATGGCAGGTGAAGCTCAGAAACTTGATTGAACCAGA GCAGTGTACGTTCTGTTTCACGGCCTCTCGAATCGATATCTGCCTCCGGAAGCGGCAGAGTCAGCGCTGG GGGGGACTGGAGGCCCCTGCTACACGAGGTGCAGTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGCCCAA CCCCTTTGGATTCAACCCCTCCAGGAGGTGGCCCCCACCCCCTGACAGGCCAGGAGGAAGCCAGGGCTGT GGAGAAGGAAAAACCCAAGGCTCGATCAGAGGACTCAGGGCTGGATGGTGTGGTGGCCCGCACCCCCTTG GAGCATGTAGCCCCAAAGCCAGACCCACACTTGGCCTCGCCCAAACCCACGTGTATGGTGCCTCCAATGC CGCACAGTCCAGTTAGTGGGGATAGTGTGGAGGAGGACGAAGAGGAAGAGAAGAAGGTGTGCCTGCCAGG CTTCACTGGCCTTGTCAACTTAGGGAACACCTGCTTCATGAATAGCGTCATTCAGTCTTTGTCCAACACT CGGGAACTTCGTGACTTCTTTCACGACCGATCCTTTGAAGCTGAGATTAACTACAATAACCCATTGGGGA CTGGTGGGCGCCTCGCCATTGGCTTTGCTGTGCTGCTCCGGGCCCTATGGAAGGGTACTCACCAAGCCTT TCAGCCCTCCAAGCTAAAGGCCATTGTGGCAAGCAAGGCCAGCCAGTTCACAGGCTATGCACAGCATGAT GCTCAAGAGTTCATGGCTTTCTTGTTGGATGGGCTACATGAAGACCTCAATCGAATCCAAAACAAACCCT ACACAGAGACTGTGGACTCGGACGGGCGGCCCGATGAGGTGGTAGCCGAGGAAGCATGGCAGCGGCACAA GATGAGAAATGATTCATTCATTGTGGACCTGTTTCAGGGCCAGTACAAGTCAAAGCTGGTGTGCCCTGTG TGTGCCAAGGTCTCCATCACTTTTGACCCGTTCCTTTATCTGCCGGTACCCTTGCCACAAAAGCAAAAGG TTCTCCCCATATTTTATTTTGCCAGGGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGTGTCAGCAA GGAGAACTCCAGCGCGAGTGAAGTGTTGGACTCCCTCTCTCAGAGTGTCCACGTGAAGCCTGAGAACCTG CGCCTAGCCGAGGTAATTAAGAACCGTTTCCACCGTGTTTTCTTGCCCTCCCACTCACTGGACGCTGTGT CCCCCACGGACGTGCTCCTCTGCTTTGAGCTGCTCTCCCCAGAACTGGCTAAGGAGCGGGTAGTAGTGCT GGAGGTGCAGCAGCGCCCCCAGGTACCCAGCATCCCTATCTCCAAGTGCGCAGCCTGCCAGCGGAAGCAG CAATCAGAAGAAGAAAAGCTGAAGCGCTGTACCCGTTGCTACCGTGTGGGCTACTGCAACCAGTTCTGCC AGAAAACCCATTGGCCTGACCACAAAGGCCTCTGCCGCCCTGAGAACATTGGCTACCCCTTCCTGGTCAG TGTGCCTGCTTCACGCCTCACTTATGCCCGTCTTGCTCAGCTACTAGAAGGTTATGCCCGGTACTCTGTG AGTGTATTCCAACCGCCCTTCCAGCCTGGCCGCATGGCTTTGGAATCGCAGAGCCCTGGCTGTACCACGT TGCTTTCAACCAGCTCTCTGGAGGCTGGGGACAGTGAAAGAGAACCCATTCAGCCTTCTGAGCTCCAGCT GGTGACCCCTGTGGCTGAAGGGGATACAGGGGCTCACCGAGTATGGCCGCCTGCTGATAGGGGTCCTGTG CCTAGCACCAGTGGACTCTCTTCTGAGATGCTGGCCAGTGGGCCTATCGAAGGTTGTCCCTTGCTTGCTG GTGAGAGGGTATCTCGGCCTGAAGCTGCTGTGCCTGGGTACCAACACTCAAGTGAATCTGTGAATACCCA CACGCCCCAGTTCTTCATCTATAAAATTGATGCATCAAACCGTGAGCAGCGGCTGGAGGACAAAGGGGAG ACACCATTGGAGCTAGGTGATGACTGTAGCCTGGCTCTGGTGTGGCGGAACAATGAACGCCTGCAGGAGT TTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAAGATCCAGGCTCTGCTGGTGAGGCTGCCCGTGC TGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTTACACGGCCTGAAGTGCTGGCACCTGAGGAGGCC TGGTACTGCCCACAGTGCAAACAGCATCGTGAGGCCTCCAAACAGCTGCTGTTGTGGCGCCTACCGAACG TGCTGATTGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTCATTTGGCGAGACAAGATCAATGACTTGGT GGAGTTTCCTGTTCGGAACCTGGACTTGAGCAAGTTCTGTATCGGTCAGAAAGAGGAGCAGCTGCCTAGC TATGACCTGTATGCTGTCATCAACCACTACGGAGGCATGATCGGTGGCCACTATACTGCTTGTGCACGGC TGCCCAATGATCGCAGTAGCCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACGGTGACAAC AGTAGACGAAAGCCAGGTCGTGACGCGCTATGCCTATGTTCTCTTCTACCGTCGTCGGAACTCTCCTGTG GAGAGACCCCCCAGGGCAAGTCACTCTGAACACCACCCAGACCTAGGCCCTGCAGCTGAGGCTGCTGCCA GCCAGGCTTCCCGGATTTGGCAGGAGCTCGAGGCCGAGGAGGAGATGGTACCTGAGGGGCCTGGGCCTCT GGGTCCTTGGGGGCCCCAAGACTGGGTGGGGCCCCCGCCACGTGGCCCTACCACACCAGACGAGGGTTGC CTCCGATACTTTGTCCTGGGTACCGTGGCGGCTTTGGTGGCCCTTGTGCTCAACGTATTCTATCCTCTGG TATCTCAGAGTCGCTGGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211946 representing NM_027804
Red=Cloning site Green=Tags(s) MSAGASATGPRRGPPGLEEATSKKKQKDRANLESKDGDARRVSLPRKEPTKDELLLDWRQSADEVIVKLR VGTGPVRLEDVDAAFTDTDCVVRLPDGRQWGGVFFAEIQSSCTKVQARKGGLLQLVLPKKVPLLTWPSLL KPLGTQELVPGLQCQENGQELSPIALEPGSEPRRAKQEARNQKRAQGRGEVGSGAGPGTQAGPSAKRAVH LRRGPEGEGSMDGPGPQGDAPSFLSDSATQVEAEEKLCAPPMNTQTSLLSSEKSLALLTVEKTVSPRNDP VAPVMVQDRDPEPEQEDQVKEEMALGADPTALVEEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEIRRDS SRVLFREQDFTLIFQTRDGNFLRLHPGCGPHTIFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRW GGLEAPATRGAVGGAKVAVPTGPTPLDSTPPGGGPHPLTGQEEARAVEKEKPKARSEDSGLDGVVARTPL EHVAPKPDPHLASPKPTCMVPPMPHSPVSGDSVEEDEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNT RELRDFFHDRSFEAEINYNNPLGTGGRLAIGFAVLLRALWKGTHQAFQPSKLKAIVASKASQFTGYAQHD AQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPV CAKVSITFDPFLYLPVPLPQKQKVLPIFYFAREPHSKPIKFLVSVSKENSSASEVLDSLSQSVHVKPENL RLAEVIKNRFHRVFLPSHSLDAVSPTDVLLCFELLSPELAKERVVVLEVQQRPQVPSIPISKCAACQRKQ QSEEEKLKRCTRCYRVGYCNQFCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSV SVFQPPFQPGRMALESQSPGCTTLLSTSSLEAGDSEREPIQPSELQLVTPVAEGDTGAHRVWPPADRGPV PSTSGLSSEMLASGPIEGCPLLAGERVSRPEAAVPGYQHSSESVNTHTPQFFIYKIDASNREQRLEDKGE TPLELGDDCSLALVWRNNERLQEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEA WYCPQCKQHREASKQLLLWRLPNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPS YDLYAVINHYGGMIGGHYTACARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPV ERPPRASHSEHHPDLGPAAEAAASQASRIWQELEAEEEMVPEGPGPLGPWGPQDWVGPPPRGPTTPDEGC LRYFVLGTVAALVALVLNVFYPLVSQSRWR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027804 |
| ORF Size | 4080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027804.4, NP_082080.3 |
| RefSeq Size | 4727 bp |
| RefSeq ORF | 4083 bp |
| Locus ID | 71472 |
| UniProt ID | Q3UJD6 |
| MW | 151 kDa |
| Gene Summary | Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates (By similarity). Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Exhibits a preference towards 'Lys-63'-linked ubiquitin chains (By similarity). Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224319 | Usp19 (untagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 1, (10ug) |
CNY 17,390.00 |
|
| MG211946 | Usp19 (tGFP-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19) |
CNY 11,880.00 |
|
| MR211946L3 | Lenti ORF clone of Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 1 |
CNY 12,730.00 |
|
| MR211946L4 | Lenti ORF clone of Usp19 (mGFP-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 1 |
CNY 12,730.00 |
