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Aox1 (NM_009676) Mouse Tagged ORF Clone
CAT＃: MR211930
- TrueORF®
Aox1 (Myc-DDK-tagged) - Mouse aldehyde oxidase 1 (Aox1)
ORF Plasmid: tGFP
"NM_009676" in other vectors (4)
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CNY 9,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI196512; AI255253; Ao; Aox-1; Aox-2; Aox2; Moro; Ro |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211930 representing NM_009676
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCCATTCAGCTGCTCTTCTACGTGAATGGCCAGAAGGTGGTAGAAAAAAATGTCGATCCTGAAA TGATGCTTTTACCATACCTGAGGAAGAATCTCCGACTCACAGGAACTAAGTATGGCTGTGGAGGCGGGGG CTGTGGGGCCTGCACAGTGATGATCTCGCGGTACAACCCCAGCACCAAGGCGATCAGGCATCATCCTGTC AATGCCTGTCTGACCCCCATCTGCTCTCTACATGGTACAGCAGTCACCACGGTAGAAGGCTTAGGCAACA CCAGGACCAGGCTTCATCCTATTCAGGAGAGAATTGCCAAGTGTCACGGCACCCAGTGTGGATTCTGTAC TCCTGGGATGGTGATGTCCATGTACGCACTGCTCAGGAACCATCCAGAGCCCACTCTAGATCAGTTAACT GATGCCCTTGGTGGGAATCTGTGCCGCTGCACTGGATATAGGCCCATAATTGATGCCTGCAAGACTTTCT GTAAAGCCTCTGGCTGCTGTCAAAGTAAAGAAAATGGGGTGTGCTGTTTGGATCAAGAAATAAATGGATT GGCAGAATCCCAGGAAGAAGATAAGACAAGTCCAGAACTGTTCTCAGAAGAGGAATTTCTGCCACTGGAC CCGACCCAAGAGCTGATATTTCCTCCCGAGCTAATGAGAATAGCTGAGAAACAGCCACCAAAGACCAGAG TGTTTTATGGTGAGAGGGTGACATGGATTTCCCCCGTGACTCTGAAGGAACTTGTGGAAGCTAAATTCAA GTATCCCCAGGCCCCTATTGTCATGGGGTACACTTCTGTGGGACCTGAAGTAAAGTTTAAAGGTGTCTTC CACCCCATCATAATTTCTCCTGACAGAATTGAAGAGCTGGGTGTCATAAGCCAGGCCAGGGATGGGCTGA CCCTGGGTGCTGGCCTCAGCCTGGATCAGGTGAAGGACATTCTGGCTGATATAGTCCAGAAGCTTCCAGA AGAGAAGACACAGACATACCGTGCTCTCCTGAAGCACCTGAGAACTCTGGCTGGCTCCCAGATCAGGAAC ATGGCTTCTCTAGGGGGCCACATTGTGAGCAGACATCTGGACTCAGATCTGAATCCCCTTCTGGCTGTGG GTAACTGTACCCTCAACTTACTGTCCAAAGATGGAGAACGGCGGATCCCTTTAAGTGAAGAGTTTCTCCG AAAGTGTCCTGAAGCAGATCTTAAGCCTCAGGAAGTCTTGGTCTCAGTGAACATCCCCTGGTCCAGGAAG TGGGAGTTTGTGTCAGCCTTCCGTCAAGCGCAAAGACAACAGAATGCACTAGCAATAGTCAACTCCGGAA TGAGAGTCCTTTTTAGAGAAGGAGGTGGCGTCATTGAAGAGTTATCCATTTTGTATGGAGGTGTCGGTTC AACTATCATCAGTGCCAAGAACTCCTGTCAGAGACTCATTGGGAGGCCCTGGAATGAAGGGATGCTGGAC ACAGCCTGTAGGCTGGTTTTGGATGAAGTCACCCTTGCAGCCTCAGCTCCTGGTGGGAAGGTGGAGTTCA AGAGGACCCTCATCATCAGCTTCCTTTTCAAGTTCTACCTGGAGGTGTCACAGGGTTTGAAGAGGGAGGA CCCAGGTCACTCTCCTAGCCTGGCAGGCAACCATGAGAGTGCTTTAGATGATCTTCATTCAAAACATCCC TGGAGAACATTAACCCACCAGAATGTAGATCCAGCACAGCTGCCTCAGGACCCCATTGGACGTCCCATCA TGCACCTTTCTGGGATTAAACATGCCACGGGCGAGGCCATCTACTGTGACGACATGCCTGCAGTAGACCG GGAGCTTTTCCTCACTTTTGTAACAAGTTCAAGAGCACACGCTAAGATTGTGTCCATTGATCTGTCGGAA GCTCTCAGCCTGCCTGGTGTGGTGGACATCATTACTGCAGATCATCTTCAGGAAGCAAACACCTTCGGCA CAGAGACATTTCTGGCCACAGATGAGGTACACTGCGTGGGCCATCTTGTCTGTGCTGTGATTGCAGATTC TGAGACACGGGCAAAGCAAGCGGCGAAGCAAGTGAAGGTGGTCTACCAAGACTTGGCGCCTCTGATCCTA ACGATTGAGGAAGCTATACAACACAAGTCCTTCTTCAAGTCAGAACGGAAGCTGGAGTGTGGGAATGTTG ACGAAGCATTTAAAATCGTTGATCAAATTCTTGAAGGTGAAATACACATAGGCGGCCAGGAACATTTTTA TATGGAAACCCAAAGCATGCTTGTTGTTCCCAAAGGAGAGGATGGAGAGATTGACATCTATGTGTCTACA CAGTTTCCCAAATATATACAGGATATAGTCGCTGCAACCTTGAAGCTCTCAGCCAACAAGGTCATGTGTC ATGTAAGGCGTGTTGGCGGGGCATTTGGAGGGAAGGTGGGCAAGACCAGCATCTTGGCAGCCATCACTGC ATTTGCTGCTAGCAAACACGGTCGCGCAGTCCGCTGCATTCTGGAACGAGGAGAAGACATGTTAATAACT GGAGGCCGCCATCCTTACCTTGGAAAGTATAAAGCTGGATTCATGAATGACGGCAGAATCTTGGCCCTGG ACGTGGAGCACTACTGCAATGGAGGGTGCTCCCTGGATGAGTCACTATGGGTGATAGAAATGGGGCTTCT GAAGCTGGACAACGCTTACAAGTTTCCCAACCTACGCTGCCGGGGCTGGGCCTGCAGAACCAACCTTCCA TCCAACACTGCTCTGCGTGGGTTTGGCTTTCCTCAGGCAGGGCTGGTCACCGAAGCCTGTATCACAGAAG TGGCAATCAAATGTGGCCTGTCCCCTGAGCAGGTTCGAACCATAAATATGTACAAGCACGTTGATACTAC CCATTACAAGCAAGAGTTCAGCGCCAAGGCCCTCTCTGAGTGCTGGAGAGAGTGCATGGCCAAGTGTTCC TACTTTGAGAGGAAAGCAGCCATAGGAAAATTCAACGCAGAGAATTCCTGGAAGAAGAGAGGAATGGCTG TGATTCCCCTGAAGTTTCCTGTGGGTATTGGATCAGTAGCCATGGGACAGGCAGCTGCCTTGGTTCATAT TTATTTGGATGGCTCTGCACTGGTCTCTCATGGTGGAATTGAGATGGGGCAGGGTGTGCACACTAAAATG ATTCAGGTGGTCAGCCGGGAACTAAGGATGCCGATGTCCAGTGTCCACCTGCGTGGGACAAGCACAGAAA CCGTCCCCAACACAAATGCCTCTGGAGGCTCTGTGGTGGCAGATCTCAATGGACTGGCAGTAAAGGATGC CTGTCAGACCCTTCTAAAACGCCTTGAACCCATCATCAGCAAGAATCCTCAGGGAACTTGGAAGGATTGG GCCCAGACTGCTTTTGACCAAAGCATCAGTCTCTCGGCTGTTGGATATTTCAGGGGTTATGAGTCGAATA TAGACTGGGAGAAAGGGGAAGGTCATCCCTTCGAATACTTTGTGTTTGGAGCTGCCTGCTCAGAGGTTGA AATAGACTGCCTGACTGGGGACCATAAGAATATCAGAACAAACATCGTGATGGATGTTGGCCACAGCATA AACCCAGCCCTTGACATAGGTCAGGTTGAAGGTGCATTTATTCAAGGAATGGGACTTTACACAATAGAGG AGCTGAGTTACTCTCCTCAGGGCACTCTATACAGTCGTGGTCCAAACCAATACAAGATTCCTGCCATCTG TGACATCCCCACGGAAATGCACATTTCTTTTTTGCCCCCATCTGAACACTCAAACACCCTGTATTCATCT AAGGGCCTGGGAGAGTCTGGGGTGTTTCTGGGATGTTCGGTATTTTTTGCCATCCATGATGCAGTGAAGG CAGCGCGGCAGGAGAGAGGCATCTCTGGACCATGGAAACTCAACAGTCCTCTGACTCCAGAGAAAATCAG AATGGCCTGTGAAGATAAGTTCACCAAAATGATCCCAAGAGATGAGCCTGGATCCTATGTTCCCTGGAAC ATACCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211930 representing NM_009676
Red=Cloning site Green=Tags(s) MDPIQLLFYVNGQKVVEKNVDPEMMLLPYLRKNLRLTGTKYGCGGGGCGACTVMISRYNPSTKAIRHHPV NACLTPICSLHGTAVTTVEGLGNTRTRLHPIQERIAKCHGTQCGFCTPGMVMSMYALLRNHPEPTLDQLT DALGGNLCRCTGYRPIIDACKTFCKASGCCQSKENGVCCLDQEINGLAESQEEDKTSPELFSEEEFLPLD PTQELIFPPELMRIAEKQPPKTRVFYGERVTWISPVTLKELVEAKFKYPQAPIVMGYTSVGPEVKFKGVF HPIIISPDRIEELGVISQARDGLTLGAGLSLDQVKDILADIVQKLPEEKTQTYRALLKHLRTLAGSQIRN MASLGGHIVSRHLDSDLNPLLAVGNCTLNLLSKDGERRIPLSEEFLRKCPEADLKPQEVLVSVNIPWSRK WEFVSAFRQAQRQQNALAIVNSGMRVLFREGGGVIEELSILYGGVGSTIISAKNSCQRLIGRPWNEGMLD TACRLVLDEVTLAASAPGGKVEFKRTLIISFLFKFYLEVSQGLKREDPGHSPSLAGNHESALDDLHSKHP WRTLTHQNVDPAQLPQDPIGRPIMHLSGIKHATGEAIYCDDMPAVDRELFLTFVTSSRAHAKIVSIDLSE ALSLPGVVDIITADHLQEANTFGTETFLATDEVHCVGHLVCAVIADSETRAKQAAKQVKVVYQDLAPLIL TIEEAIQHKSFFKSERKLECGNVDEAFKIVDQILEGEIHIGGQEHFYMETQSMLVVPKGEDGEIDIYVST QFPKYIQDIVAATLKLSANKVMCHVRRVGGAFGGKVGKTSILAAITAFAASKHGRAVRCILERGEDMLIT GGRHPYLGKYKAGFMNDGRILALDVEHYCNGGCSLDESLWVIEMGLLKLDNAYKFPNLRCRGWACRTNLP SNTALRGFGFPQAGLVTEACITEVAIKCGLSPEQVRTINMYKHVDTTHYKQEFSAKALSECWRECMAKCS YFERKAAIGKFNAENSWKKRGMAVIPLKFPVGIGSVAMGQAAALVHIYLDGSALVSHGGIEMGQGVHTKM IQVVSRELRMPMSSVHLRGTSTETVPNTNASGGSVVADLNGLAVKDACQTLLKRLEPIISKNPQGTWKDW AQTAFDQSISLSAVGYFRGYESNIDWEKGEGHPFEYFVFGAACSEVEIDCLTGDHKNIRTNIVMDVGHSI NPALDIGQVEGAFIQGMGLYTIEELSYSPQGTLYSRGPNQYKIPAICDIPTEMHISFLPPSEHSNTLYSS KGLGESGVFLGCSVFFAIHDAVKAARQERGISGPWKLNSPLTPEKIRMACEDKFTKMIPRDEPGSYVPWN IPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009676 |
| ORF Size | 3999 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009676.2, NP_033806.2 |
| RefSeq Size | 4382 bp |
| RefSeq ORF | 4002 bp |
| Locus ID | 11761 |
| UniProt ID | O54754 |
| MW | 147.1 kDa |
| Gene Summary | Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Also plays a role in the reductive metabolism of the xenobiotic imidacloprid (IMI) via its nitroreduction to nitrosoguanidine (IMI-NNO) and aminoguanidine (IMI-NNH(2)). Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis. Cannot use xanthine and hypoxanthine as substrate.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224256 | Aox1 (untagged) - Mouse aldehyde oxidase 1 (Aox1), (10ug) |
CNY 17,100.00 |
|
| MG211930 | Aox1 (tGFP-tagged) - Mouse aldehyde oxidase 1 (Aox1) |
CNY 12,830.00 |
|
| MR211930L3 | Lenti ORF clone of Aox1 (Myc-DDK-tagged) - Mouse aldehyde oxidase 1 (Aox1) |
CNY 13,590.00 |
|
| MR211930L4 | Lenti ORF clone of Aox1 (mGFP-tagged) - Mouse aldehyde oxidase 1 (Aox1) |
CNY 13,590.00 |
