United States
Germany
Japan
United Kingdom
China
Stag1 (NM_009282) Mouse Tagged ORF Clone
CAT＃: MR211863
- TrueORF®
Stag1 (Myc-DDK-tagged) - Mouse stromal antigen 1 (Stag1)
"NM_009282" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 13,136.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU045003; SA-1; Scc3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211863 representing NM_009282
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTACTTCAGAGTTACCAGTGTTACAGGATTCAACTAATGAAACTACTGCCCACTCTGATGCTGGCA GCGAACTTGAAGAAACAGAGGTCAAAGGAAAAAGAAAAAGGGGTCGTCCTGGCCGGCCTCCATCAACAAA TAAGAAACCTCGAAAATCTCCAGGAGAAAAGAGCAGAATTGAAGCTGGAATTCGAGGAGCAGGTCGTGGA AGAGCCAATGGGCACCCCCAACAGAACGGCGACGGGGATCCTGTCACCTTATTTGAGGTGGTGAAGCTGG GGAAGAGTGCAATGCAGTCCGTGGTGGATGACTGGATTGAATTATATAAACAAGACAGGGACATCGCACT TCTGGATTTAATCAACTTTTTTATCCAGTGTTCAGGATGTCGAGGTACGGTCAGAATAGAGATGTTTCGA AATATGCAGAACGCAGAAATAATCAGAAAAATGACTGAAGAATTTGATGAGGACAGTGGTGACTACCCCC TTACAATGCCTGGTCCTCAGTGGAAAAAATTTCGTTCCAATTTTTGTGAATTTATTGGAGTCCTGATTCG ACAGTGTCAATATAGCATAATTTATGATGAATATATGATGGACACCGTAATTTCCCTTTTGACTGGTTTG TCAGACTCCCAAGTCAGAGCTTTTAGGCATACAAGTACCCTTGCTGCAATGAAGCTGATGACTGCTCTGG TGAATGTTGCTTTAAACCTCAGTATTCATCAAGATAATACACAGAGGCAATATGAAGCTGAGAGAAATAA AATGATTGGGAAGAGAGCCAATGAAAGACTGGAGTTACTACTTCAGAAACGTAAAGAGCTACAAGAAAAT CAAGATGAAATTGAAAATATGATGAACTCTATCTTTAAGGGTATATTTGTTCATCGATACCGTGATGCAA TTGCTGAAATTCGAGCCATCTGTATTGAAGAAATTGGAGTGTGGATGAAAATGTATAGCGATGCCTTCCT AAATGACAGTTACTTGAAATATGTAGGATGGACTCTTCACGATAGGCAAGGGGAAGTCAGACTGAAGTGT TTGAAAGCTCTACAAAGCCTGTATACCAATAGAGAATTATTCCCCAAATTGGAGCTATTTACAAATCGAT TCAAGGATCGCATTGTATCAATGACTCTTGACAAAGAATATGATGTTGCTGTGGAAGCAATCCGATTGGT TACTCTGATCCTTCATGGCAGTGAAGAAGCTCTTTCCAACGAAGACTGTGAAAATGTTTACCATTTGGTG TATTCAGCACATCGCCCTGTTGCTGTGGCAGCTGGAGAGTTCCTACACAAAAAGCTGTTCAGCAGACATG ACCCACAAGCAGAGGAAGCGTTAGCGAAGAGGAGAGGAAGGAACAGTCCAAACGGGAACCTCATTAGGAT GCTTGTTCTTTTCTTTCTGGAAAGTGAGTTACATGAACATGCAGCCTACTTGGTGGACAGCTTGTGGGAG AGCTCTCAAGAACTGTTGAAAGACTGGGAATGTATGACAGAGTTACTATTAGAAGAACCTGTTCAAGGAG AAGAAGCAATGTCTGACCGTCAAGAGAGTGCTCTTATAGAGCTAATGGTCTGTACAATTCGTCAGGCAGC TGAGGCACATCCTCCAGTGGGAAGGGGTACTGGCAAGAGAGTGCTAACAGCCAAAGAAAGGAAAACTCAA ATTGATGATAGGAACAAATTAACTGAACATTTTATCATTACACTTCCTATGTTACTATCAAAGTATTCTG CAGATGCTGAGAAGGTAGCAAACTTGCTGCAAATTCCACAGTATTTTGATCTAGAAATCTACAGCACAGG TCGGATGGAAAAGCACCTGGATGCTTTATTAAAACAAATTAAGTTTGTTGTAGAGAAACATGTAGAATCA GATGTCCTAGAAGCCTGCAGTAAAACCTACAGCATTTTATGCAGTGAAGAGTATACCATTCAGAATCGAG TTGATATTGCTCGGAGCCAACTGATTGATGAATTTGTAGATCGATTCAATCATTCTGTAGAAGACCTGTT GCAAGAGGGTGAAGAAGCTGACGATGATGATATCTACAATGTTCTTTCCACCTTAAAGCGTTTAACTTCT TTTCACAATGCTCATGATCTCACTAAATGGGATCTATTTGGTAATTGCTACAGACTATTGAAGACTGGAA TTGAACATGGAGCTATGCCAGAACAGATAGTCGTACAAGCACTGCAGTGTTCCCATTACTCAATTCTGTG GCAGTTGGTGAAAATTACTGACGGATCTCCTTCCAAAGAGGATTTGTTGGTATTGAGGAAAACAGTGAAG TCTTTTCTGGCTGTTTGCCAACAGTGCCTATCTAATGTTAATACTCCAGTTAAGGAACAGGCTTTCATGC TGCTCTGTGATCTTTTAATGATTTTTAGCCACCAATTAATGACAGGTGGCAGAGAGGGCCTTCAGCCTTT GGTGTTTAATCCAGATACTGGACTCCAGTCTGAACTCCTCAGTTTTGTGATGGATCATGTTTTCATTGAC CAAGATGAGGAAAACCAAAGCATGGAGGGTGATGAGGAAGATGAAGCTAATAAAATTGAAGCCTTACATA AAAGGAGGAACCTGCTTGCTGCCTTCAGCAAACTTATCATTTATGATATTGTTGACATGCACGCAGCTGC AGACATCTTTAAACACTACATGAAGTATTACAATGACTATGGTGATATTATTAAGGAAACACTGAGTAAG ACCAGGCAGATTGATAAAATTCAGTGTGCTAAGACTCTCATTCTCAGTCTGCAACAGTTGTTTAATGAAC TTGTTCAAGAACAAGGTCCTAATCTGGATAGGACATCTGCCCACGTCAGTGGGATTAAAGAACTGGCACG TCGTTTTGCCCTTACATTTGGATTGGACCAGATCAAGACCCGAGAAGCTGTTGCCACACTTCACAAGGAT GGAATAGAGTTTGCCTTTAAATACCAGAATCAGAAAGGACAAGAATACCCACCCCCTAATCTGGCTTTTC TAGAAGTACTAAGTGAATTTTCTTCTAAACTCCTTCGACAGGACAAGAAAACTGTTCATTCGTATCTTGA AAAATTCCTCACCGAGCAGATGATGGAAAGGAGGGAGGATGTGTGGCTTCCACTCATCTCGTATAGAAAC TCATTAGTCACTGGAGGTGAAGATGACAGGATGTCTGTGAACAGTGGAAGTAGCAGCAGTAAAACGTCCT CAGTAAGGAGTAAGAAAGGAAGACCCCCACTGCACAGAAAACGAGTAGAAGATGAAAGTCTGGATAACAC ATGGCTAAATAGGACTGACACGATGATTCAGACTCCTGGACCCTTGCCAACCCCACAGCTCACCTCCACG GTACTTAGAGAAAACAGCCGTCCCATGGGAGAGCAGATTCAGGAACCTGAGTCTGAGCATGGCTCTGAAC CAGACTTTTTACATAATCCCCAGATGCAGATCTCTTGGTTAGGCCAGCCGAAGTTAGAAGACTTGAATCG GAAGGACAGAACAGGGATGAACTACATGAAAGTAAGAGCTGGAGTCCGGCACGCCGTTCGGGGTCTAATG GAGGAAGATGCTGAGCCCATCTTTGAAGATGTGATGATGTCATCACGGAGCCAGTTAGAAGACATGAATG AAGAATTTGAAGACACCATGGTTATTGATTTGCCCCCATCAAGAAATCGCCGAGAGAGAGCTGAGCTAAG GCCAGACTTCTTTGACTCTGCAGCTATCATAGAAGATGATTCAGGATTTGGAATGCCTATGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211863 representing NM_009282
Red=Cloning site Green=Tags(s) MITSELPVLQDSTNETTAHSDAGSELEETEVKGKRKRGRPGRPPSTNKKPRKSPGEKSRIEAGIRGAGRG RANGHPQQNGDGDPVTLFEVVKLGKSAMQSVVDDWIELYKQDRDIALLDLINFFIQCSGCRGTVRIEMFR NMQNAEIIRKMTEEFDEDSGDYPLTMPGPQWKKFRSNFCEFIGVLIRQCQYSIIYDEYMMDTVISLLTGL SDSQVRAFRHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQEN QDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKC LKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLV YSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWE SSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQ IDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVES DVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTS FHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVK SFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFID QDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSK TRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKD GIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRN SLVTGGEDDRMSVNSGSSSSKTSSVRSKKGRPPLHRKRVEDESLDNTWLNRTDTMIQTPGPLPTPQLTST VLRENSRPMGEQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRAGVRHAVRGLM EEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009282 |
| ORF Size | 3774 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009282.4 |
| RefSeq Size | 6010 bp |
| RefSeq ORF | 3777 bp |
| Locus ID | 20842 |
| UniProt ID | Q9D3E6 |
| MW | 144.4 kDa |
| Gene Summary | Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224081 | Stag1 (untagged) - Mouse stromal antigen 1 (Stag1), (10ug) |
CNY 13,144.00 |
|
| MR211863L3 | Lenti ORF clone of Stag1 (Myc-DDK-tagged) - Mouse stromal antigen 1 (Stag1) |
CNY 14,440.00 |
|
| MR211863L4 | Lenti ORF clone of Stag1 (mGFP-tagged) - Mouse stromal antigen 1 (Stag1) |
CNY 14,440.00 |
