Snapc4 (BC057031) Mouse Tagged ORF Clone

CAT#: MR211838

  • TrueORF®

Snapc4 (Myc-DDK-tagged) - Mouse small nuclear RNA activating complex, polypeptide 4 (cDNA clone MGC:67204 IMAGE:6833968)

ORF Plasmid: DDK tGFP



  "BC057031" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 5730436L13Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211838 representing BC057031
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCTACCAGGAGGTGATCCGGGAAAAGCTAGCAGAGGTTAGCCAGTTGCTAGCCCAGAACCAGGAGC
AGCAGGAAGAGATCCTGTTTGATCTGTCTGGAACCAAATGCCCCAAGGTGAAGGATGGCAGAAGTCTGCC
CTCATACATGTACATAGGCCACTTCCTGAAGCCATACTTCAAGGACAAGGTTACTGGAGTGGGGCCTCCT
GCCAACGAAGAAACACGGGAGAAGGCTACCCAGGGAATCAAGGCCTTTGAACAGCTCTTGGTGACCAAGT
GGAAGCACTGGGAGAAAGCCTTGCTCAGGAAGTCTGTAGTGAGTGACCGCCTACAACGTCTGCTTCAGCC
CAAATTGCTGAAGCTGGAGTACTTACACGAAAAACAGAGCCGAGTCTCCAGTGAGTTGGAAAGGCAGGCC
CTGGAGAAGCAGATTAAGGAAGCAGAGAAGGAGATTCAGGACATCAACCAGCTCCCAGAGGAAGCATTGT
TGGGGAACCGGCTAGACAGCCACGACTGGGAGAAGATTTCCAATATTAACTTTGAAGGAGCCCGCAGTGC
AGAGGAGATCCGGAAGTTCTGGCAGAGTTCTGAGCATCCAAGCATCAGTAAGCAGGAATGGAGCACAGAA
GAAGTAGAGAGGCTGAAGGCCATCGCTGCCACACATGGCCACCTGGAGTGGCACTTGGTTGCAGAGGAAC
TTGGGACAAGCCGCAGTGCCTTTCAGTGCCTGCAGAAATTCCAGCAGTACAACAAAACCCTGAAACGCAA
GGAGTGGACAGAAGAGGAAGACCACATGCTCACCCAGCTGGTCCAAGAGATGCGAGTTGGGAACCATATC
CCATACCGCAAGATCGTCTACTTCATGGAAGGGAGAGACTCCATGCAGCTGATCTACCGTTGGACCAAGA
GCTTAGATCCCAGCCTGAAAAGGGGATTCTGGGCCCCAGAGGAAGATGCTAAATTGCTTCAAGCTGTTGC
CAAGTATGGGGCGCAGGACTGGTTTAAAATCCGGGAAGAGGTGCCAGGTAGGAGCGATGCCCAATGCAGA
GACCGCTACATCAGAAGATTACATTTCAGCTTGAAAAAGGGACGGTGGAATGCAAAAGAAGAACAACAGC
TGATCCAGCTAATAGAGAAATATGGTGTTGGTCACTGGGCAAGAATCGCTTCTGAATTGCCCCATCGGTC
AGGCTCCCAGTGTCTGAGCAAGTGGAAGATCTTGGCCAGGAAGAAACAGCATCTGCAGAGGAAGAGAGGG
CAGAGGCCCCGTCACAGTAGCCAGTGGAGCTCTAGCGGCAGCAGCAGCAGCAGCAGTGAAGACTACGGCA
GCAGCAGTGGCAGCGACGGCAGCAGCGGCAGTGAAAACTCAGATGTGGAGCTGGAGGCGTCTCTGGAGAA
GAGTAGAGCACTGACTCCTCAACAGTACAGAGTGCCAGACATTGACCTGTGGGTTCCTACCAGGCTGATC
ACCAGCCAGTCACAGAGAGAAGGGACAGGGTGCTACCCACAACACCCTGCTGTCTCCTGCTGCACTCAGG
ATGCCTCTCAAAATCACCACAAGGAAGGTTCTACCACCGTCTCTGCTGCTGAGAAAAACCAGCTGCAGGT
GCCCTATGAGACCCACAGCACTGTTCCAAGAGGAGACCGGTTCCTACACTTCTCGGACACCCACTCAGCA
AGTTTGAAGGATCCGGCCTGCAAGAGTCACACACTGATGAAGGAAAGACCGAAGCAGCCACTCCTCCCTA
GTTCACGCTCAGGGTCTGACCCTGGCAACAATACGGCTGGGCCCCATCTGCGGCAGCTATGGCATGGAAC
TTACCAGAATAAGCAGAGACGCAAGAGACAGGCTCTACATCGCAGGCTCCTTAAGCACAGGCTTTTGCTG
GCTGTGATACCCTGGGTGGGTGACATCAACCTGGCCTGCACACAAGCTCCCCGGAGACCTGCAACCGTTC
AGACTAAAGCTGACAGCATCCGGATGCAGTTAGAGTGTGCCCGCCTGGCCAGCACTCCGGTATTCACACT
GCTTATTCAGCTATTGCAGATTGATACTGCGGGCTGCATGGAGGTGGTTCGAGAGAGGAAAAGCCAGCCC
CCTGCACTGCTCCAGCCTGGCACCCGGAACACCCAGCCGCATCTCCTGCAGGCATCCTCTAATGCCAAGA
ACAACACAGGCTGCCTCCCAAGCATGACGGGGGAACAAACTGCAAAGAGAGCCAGCCACAAAGGGCGCCC
ACGACTGGGGTCATGCAGGACTGAGGCTACCCCCTTCCAAGTCCCTGTAGCAGCCCCACGTGGCCTCCGG
CCAAAGCCTAAGACTGTTTCTGAGCTGCTTCGGGAGAAGCGGCTCCGTGAGTCCCATGCCAAGAAGGCCA
CTCAGGCTCTTGGTCTCAACAGCCAGCTGCTGGTCTCCTCACCTGTGATCCTCCAGCCCCCTCTGCTGCC
AGTCCCCCATGGTTCCCCAGTAGTAGGCCCTGCAACGTCCAGTGTAGAACTCTCTGTACCTGTGGCGCCG
GTGATGGTCAGTTCAAGTCCTTCTGGCTCCTGGCCGGTGGGTGGGATCTCAGCCACAGACAAGCAGCCCC
CCAACCTACAGACCATTTCCCTAAATCCTCCCCACAAAGGGACCCAGGTTGCAGCCCCTGCTGCTTTTAG
GAGCCTAGCCTTAGCCCCCGGGCAAGTCCCCACAGGCGGCCATCTGAGTACTCTAGGACAGACCTCTACC
ACATCCCAGAAGCAGAGCCTGCCCAAGGTCCTGCCCATTCTTCGAGCTGCTCCCAGCCTCACTCAGCTGT
CTGTGCAGCCACCAGTAAGTGGACAGCCCCTGGCAACCAAGTCCAGCCTGCCTGTCAACTGGGTTCTCAC
AACTCAGAAGTTGCTCTCTGTCCAAGTGCCAGCTGTGGTGGGCCTCCCCCAGTCAGTGATGACCCCTGAG
ACCATAGGACTACAAGCAAAACAGCTGCCTTCCCCTGCCAAGACTCCTGCTTTTCTGGAACAGCCTCCAG
CCAGTACAGACACAGAACCCAAGGGACCTCAGGGCCAGGAAATACCACCCACACCTGGGCCCGAAAAGGC
AGCTTTGGACCTAAGTCTCCTTTCCCAGGAAAGTGAGGCAGCCATCGTCACATGGCTGAAGGGGTGCCAA
GGTGCTTTTGTACCCCCGCTGGGGAGCAGGATGCCCTATCATCCCCCTTCCCTGTGCAGCTTGCGGGCAT
TGTCCAGCCTCCTCCTCCAGAAACAGGACTTGGAGCAGAAGGCTTCCTCTCTGGCGGCCAGCCAGGCAGC
TGGGGCCCAACCTGATCCCAAGGCTGGAGCCCTACAAGCTTCCCTGGAGCTGGTACAGAGGCAATTCCGG
GACAACCCAGCCTACCTCCTGCTAAAGACGCGTTTCCTGGCTATCTTCTCCCTTCCTGCATTCCTGGCCA
CTCTGCCCCCCAACAGCATCCCCACCACCCTGTCACCAGACGTGGCTGTGGTTTCTGAGAGTGACAGTGA
AGACCTCGGTGACTTGGAGCTCAAGGATAGGGCTAGACAGTTGGACTGCATGGCCTGCAGAGTGCAAGCA
AGCCCAGCAGCCCCAGACCCTGTACAGAGAGCTCCAAGTCCTGGTGAGGTTTCTGCTCCCTCCCCTCTGG
ATGCTTCTGATGGCCTTGATGACCTTAACGTGCTCAGGACTCGACGTGCTCGGCATTCCCGGAGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR211838 representing BC057031
Red=Cloning site Green=Tags(s)

MVYQEVIREKLAEVSQLLAQNQEQQEEILFDLSGTKCPKVKDGRSLPSYMYIGHFLKPYFKDKVTGVGPP
ANEETREKATQGIKAFEQLLVTKWKHWEKALLRKSVVSDRLQRLLQPKLLKLEYLHEKQSRVSSELERQA
LEKQIKEAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGARSAEEIRKFWQSSEHPSISKQEWSTE
EVERLKAIAATHGHLEWHLVAEELGTSRSAFQCLQKFQQYNKTLKRKEWTEEEDHMLTQLVQEMRVGNHI
PYRKIVYFMEGRDSMQLIYRWTKSLDPSLKRGFWAPEEDAKLLQAVAKYGAQDWFKIREEVPGRSDAQCR
DRYIRRLHFSLKKGRWNAKEEQQLIQLIEKYGVGHWARIASELPHRSGSQCLSKWKILARKKQHLQRKRG
QRPRHSSQWSSSGSSSSSSEDYGSSSGSDGSSGSENSDVELEASLEKSRALTPQQYRVPDIDLWVPTRLI
TSQSQREGTGCYPQHPAVSCCTQDASQNHHKEGSTTVSAAEKNQLQVPYETHSTVPRGDRFLHFSDTHSA
SLKDPACKSHTLMKERPKQPLLPSSRSGSDPGNNTAGPHLRQLWHGTYQNKQRRKRQALHRRLLKHRLLL
AVIPWVGDINLACTQAPRRPATVQTKADSIRMQLECARLASTPVFTLLIQLLQIDTAGCMEVVRERKSQP
PALLQPGTRNTQPHLLQASSNAKNNTGCLPSMTGEQTAKRASHKGRPRLGSCRTEATPFQVPVAAPRGLR
PKPKTVSELLREKRLRESHAKKATQALGLNSQLLVSSPVILQPPLLPVPHGSPVVGPATSSVELSVPVAP
VMVSSSPSGSWPVGGISATDKQPPNLQTISLNPPHKGTQVAAPAAFRSLALAPGQVPTGGHLSTLGQTST
TSQKQSLPKVLPILRAAPSLTQLSVQPPVSGQPLATKSSLPVNWVLTTQKLLSVQVPAVVGLPQSVMTPE
TIGLQAKQLPSPAKTPAFLEQPPASTDTEPKGPQGQEIPPTPGPEKAALDLSLLSQESEAAIVTWLKGCQ
GAFVPPLGSRMPYHPPSLCSLRALSSLLLQKQDLEQKASSLAASQAAGAQPDPKAGALQASLELVQRQFR
DNPAYLLLKTRFLAIFSLPAFLATLPPNSIPTTLSPDVAVVSESDSEDLGDLELKDRARQLDCMACRVQA
SPAAPDPVQRAPSPGEVSAPSPLDASDGLDDLNVLRTRRARHSRR

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057031
ORF Size 3705 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057031.1
RefSeq Size 4473 bp
RefSeq ORF 3707 bp
Locus ID 227644
MW 164 kDa
Gene Summary Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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