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Wnk4 (NM_175638) Mouse Tagged ORF Clone
CAT＃: MR211822
- TrueORF®
Wnk4 (Myc-DDK-tagged) - Mouse WNK lysine deficient protein kinase 4 (Wnk4)
ORF Plasmid: tGFP
"NM_175638" in other vectors (4)
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CNY 8,512.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010002J11Rik; Pha2b; Prkwnk4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211822 representing NM_175638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGCACCTCGAAATACGGAGACTGGGGTCCCCATGTCCCAAACCGAGGCTGACCTGGCTCTGCGGC CATCTCCGGCTCTCACCTCTACGGGGCCGACCCGCCTGGGGCCACCTCCTCGCCGAGTGCGCCGCTTCTC TGGGAAGGCTGAGCCTCGGCCGCGCTCTTCGAGACCTAGCCGCCGCAGCTCAGTCGATCTGGGACTGCTG AGCTCCTGGTCTCAACCAGCCTCACTCCTTCCGGAACCCCCGGATCCTCCAGACTCCGCTGGCCCCACGA GGAGCCCACCTTCAAGCTCTAAAGAACCCCCCGAGGGCACATGGATGGGGGCAGCTCCCGTGAAGGCTGT GGACTCTGCATGTCCTGAGCTTACGGGATCTTCAGGAGGTCCAGGGTCCCGGGAGCCACCGAGGGTTCCT GATGCTGCAGCCCGGGAACGGCGGCGGGAGCAGGAAGAAAAAGAGGACACAGAAACCCAGGCTGTGGCAA CGTCTCCGGACGGCCGATACCTCAAGTTTGACATTGAGATTGGAAGAGGTTCCTTCAAGACCGTGTATCG AGGGCTAGACACCGACACCACGGTAGAGGTGGCCTGGTGTGAGCTGCAGACCCGGAAACTCTCTCGGGCC GAGCGGCAGCGTTTCTCCGAGGAAGTTGAGATGCTCAAGGGGCTGCAGCACCCCAACATCGTCCGCTTCT ATGACTCCTGGAAGTCGGTGCTGAGGGGCCAGGTTTGCATCGTGCTGGTCACAGAACTCATGACCTCGGG CACGCTCAAGACGTACCTGAGGCGGTTCCGCGAAATGAAGCCCCGAGTCCTTCAGCGCTGGAGTCGCCAG ATTCTACGGGGACTTCATTTCCTTCATTCCCGAGTGCCCCCCATCCTGCACCGAGATCTCAAGTGTGACA ACGTCTTCATTACTGGCCCTTCAGGCTCTGTCAAAATCGGAGACCTCGGACTGGCCACGCTCAAGCGCGC CTCCTTTGCCAAGAGCGTCATTGGGACCCCGGAATTCATGGCTCCTGAGATGTACGAGGAAAAGTACGAC GAGGCTGTGGATGTGTACGCCTTTGGCATGTGCATGCTGGAGATGGCTACGTCTGAGTATCCCTACTCCG AGTGTCAGAATGCAGCACAAATCTACCGCAAGGTCACTTCGGGCACAAAGCCCAACAGCTTCTACAAGGT GAAGATGCCAGAGGTGAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAACGAGAGGTTCACCATC CAGGATCTCCTGGCCCATGCGTTCTTCCGCGAGGAACGAGGTGTGCACGTGGAGCTGGCAGAGGAAGATG ATGGAGAGAAGCCGGGCCTCAAGCTCTGGCTGCGCATGGAGGATGCGCGGCGCGGGGGGCGTCCACGAGA CAACCAGGCCATCGAGTTCCTGTTTCAGCTCGGCCGGGACGCTGCGGAGGAGGTGGCTCAGGAGATGGTA GCCTTGGGCTTAGTCTGTGAAGCTGACTACCAGCCAGTGGCCCGTGCAGTTCGAGAACGGGTTGCTGCTA TCCAGCGGAAGCGGGAGAAGCTTCGAAAAGCTAGGGAATTGGAGGTTCTCCCACCAGACTCAGGACCTCC TCCAGCAACTGTGTCTCTGGCTCCGGGTCCCCCCAGTGCCTTCCCCCCAGAGCCTGAGGAGCCAGAGGCT GACCAGCATCAATCCTTCCTCTTCCGCCATGCCAGCTACTCATCAACTACATCTGATTGCGAGACTGATG GCTACCTCAGTTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCCTGGGGGGTTACCATC CAGCCCCGCTGAATCCCATCTCTGCTTGCCCTCGGGTTTTGCCTTGTCTATTCCACGGTCTGGCCCTGGC AGTGACTTTTCTCCTGGGGATAGCTACGCCTCAGATGCGGCATCAGGCCTTAGTGACATGGGAGAAGGGG GGCAAATGAGGAAAAATCCAGTGAAGACTCTTCGACGGAGACCTCGATCCCGGCTCCGGGTCACTAGTGT CTCAGACCAGAGCGACAGAGTTGTCGAGTGTCAGCTGCAGACTCACAACAGCAAGATGGTGACGTTCCGA TTTGATCTGGATGGGGACAGCCCAGAAGAAATTGCAGCTGCCATGGTATACAATGAGTTCATCCTGCCCT CGGAGCGAGATGGATTCCTGAGCCGGATCCGGGAGATTATCCAGCGAGTGGAGACCCTGCTGAAGAGAGA TGCTGGTCCCCCAGAGGCTGCTGAAGATGCCCTGAGCCCCCAGGAAGAGCCAGCAGCCCTGCCTGCCCTC CCAGGCCCACCCAATGCAGAGCCCCAGAGAAGCATCTCCCCAGAACAGAGGAGCTGGGCAGCCTTCTCCA CCTCTCCATCTTCTCCTGGCACCCCCTTGTCCCCTGGGGCCCCCTTTTCCCCTGGGACCCCTCCTGTCTT CCCATGCCCCATCTTTCCTATCACTTCACCCTCATGCTATCCCTGCCCATTCTCCCAGGTCTCTTCAAAC CCCTATCCACAGGCCCCCAGTTCCCTGCTTCCCTTATCCTCCAGTGCTTCTCAGGTTCCACTTCCATCTT CCTCTCTTCCCATCAGCGCTCCCCTCCCATTCTCTCCTAGTTATCCCCAAGACCCTCTTAGCCCCACTTC TCTTCCCGTCTGCCCCTCTCCTCCCTCTCTTCCCTCTACCACAGCAGCCCCTCTCCTCTCTCTGGCTAGT GCCTTCTCTCTGGCTGTGATGACTGTGGCCCAGTCCCTGCTGTCCCCATCCCCTGGACTTCTTTCTCAGT CTCCTCCAGCCCCTCCAGGTCCTCTGCCTAGCCTGCCCCTTTCCCTTGCTTCTTGTGACCAGGAGAGCCT TTCGGCCCAAACAGCTGAGACAGAGAATGAGGCTTCCCGTAATCCTGCTCAGCCACTACTGGGTGATGCT AGACTGGCACCTATATCTGAAGAGGGAAAGCCCCAGCTTGTTGGCCGTTTCCAAGTGACTTCATCTAAGG AACCAGCAGAGCCTCCCCTGCAACCAGCATCCCCAACTCTCTCCAGATCCCTAAAGCTGCCAAGCCCTCC GCTGACCTCAGAGAGCTCAGACACAGAGGACAGTGCTGCAGGAGGCCCAGAGACTAGGGAGGCTCTGGCA GAGAGTGACCGTGCAGCCGAAGGCCTGGGGGTTGCGGTCGATGATGAAAAGGATGAAGGGAAGGAACCCC TACTTGGAGGCAGTTCCCCAATCTTGAGCCATCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCT GTGTCTGAGCAGTGAGGAGTCGGAGAGCAGCGGGGAAGACGAGGAATTCTGGGCTGAGCTGCAGAACCTT CGGCAGAAGCACTTGTCGGAAGTGGAGGCACTACAGACACTACAGAAGAAGGAAATCGAGGACTTATACA GCCGGCTTGGGAAACAGCCCCCACCTGGTATTGTAGCTCCAGCTGCTATGCTGTCCTGCCGCCAGCGCCG CCTCTCGAAGGGCAGCTTTCCCACCTCCCGCCGCAACAGCCTGCAGCGCTCTGATCTCCCTGGTCCTGGC ATCATGCGAAGGAACTCTCTGAGTGGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGA CATTCGCCGGGGATATTGGCAGGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211822 representing NM_175638
Red=Cloning site Green=Tags(s) MLAPRNTETGVPMSQTEADLALRPSPALTSTGPTRLGPPPRRVRRFSGKAEPRPRSSRPSRRSSVDLGLL SSWSQPASLLPEPPDPPDSAGPTRSPPSSSKEPPEGTWMGAAPVKAVDSACPELTGSSGGPGSREPPRVP DAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRA ERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQ ILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYD EAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTI QDLLAHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMV ALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSLAPGPPSAFPPEPEEPEA DQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGLPSSPAESHLCLPSGFALSIPRSGPG SDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFR FDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPPEAAEDALSPQEEPAALPAL PGPPNAEPQRSISPEQRSWAAFSTSPSSPGTPLSPGAPFSPGTPPVFPCPIFPITSPSCYPCPFSQVSSN PYPQAPSSLLPLSSSASQVPLPSSSLPISAPLPFSPSYPQDPLSPTSLPVCPSPPSLPSTTAAPLLSLAS AFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPLSLASCDQESLSAQTAETENEASRNPAQPLLGDA RLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPSPPLTSESSDTEDSAAGGPETREALA ESDRAAEGLGVAVDDEKDEGKEPLLGGSSPILSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNL RQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPG IMRRNSLSGSSTGSQEQRASKGVTFAGDIGRM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175638 |
| ORF Size | 3666 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175638.3, NP_783569.1 |
| RefSeq Size | 4148 bp |
| RefSeq ORF | 3669 bp |
| Locus ID | 69847 |
| UniProt ID | Q80UE6 |
| MW | 132.9 kDa |
| Gene Summary | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223989 | Wnk4 (untagged) - Mouse WNK lysine deficient protein kinase 4 (Wnk4), (10ug) |
CNY 15,680.00 |
|
| MG211822 | Wnk4 (tGFP-tagged) - Mouse WNK lysine deficient protein kinase 4 (Wnk4) |
CNY 10,550.00 |
|
| MR211822L3 | Lenti ORF clone of Wnk4 (Myc-DDK-tagged) - Mouse WNK lysine deficient protein kinase 4 (Wnk4) |
CNY 11,500.00 |
|
| MR211822L4 | Lenti ORF clone of Wnk4 (mGFP-tagged) - Mouse WNK lysine deficient protein kinase 4 (Wnk4) |
CNY 11,500.00 |
