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Evc2 (NM_145920) Mouse Tagged ORF Clone
CAT＃: MR211819
- TrueORF®
Evc2 (Myc-DDK-tagged) - Mouse Ellis van Creveld syndrome 2 homolog (human) (Evc2)
ORF Plasmid: tGFP
"NM_145920" in other vectors (4)
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CNY 8,496.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110017L09Rik; Lbn; limbin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211819 representing NM_145920
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCGACGGGCCCCACGGGCGCTGGGGGACGAGCCACGTGGGTGCTGGCTGGGAATATCCTGGCCG CTGCCCTGGTACTGGGCTCGGGTCCGCGTGCACTTCCACCATCCTTCCCTGCCCTGGGCCCAGGGTCACC ATCTCGGCCCGGCCCAGCAGGACCCTGGGCAAGCTCGCAGTACAGCGATATTTCAAGAGAAGCCCGAGGT CCCTTTGAGAATGGAGTGATATTTCAAAAATGCTCACTGGTGTCTGGGCAGAGCGAATCGCAGACCATGC ACGTGCAGCTGTCCGTGAACAACACCAGGACGCCCACATCAGTCAACCTTTCGAACCTGCTCGTGCTAGA TGAGATCACTGGCCTTGCTGTGAAGGAGTCGCCTGGAAATAATACTCAGGATGGAATCCAGACTTTCAGA AAGAGCTTTCTGCAAGTGGGAGAATGCTACTCTGTCAGCTACACAGCCTCGCTGGACCCCACAGCCCTTG GGACTGGGGAGAGCCTGGATCTTCCTGCCCGGCTCATCTTTCAGAGCCCCTCACAGAACAGAACTCAGCT AAAAGCCCCCTTTACCATCACAGTGGAAGAAAAGATAATGGTTCTTCCTAACCATGGTCTTCACGCAGCA GGCTTCATCGCAGCCTTCCTCATCTCCCTCCTCCTGACCGTGGCAGCCCTGTTCTTCTTGGCGAGGGGTC GATGTCTGCAGGGAGGCATGCTGTCCAGATGCCGGATTCAGCATCCTGAGAACAAGCTGGAGCCCTCGCC CTTCACCTCTGCTAACGGTGTGAGCCAGGACCTTTCCCTCAATGACCAAGTGGTTGCCATCCTGACTTCT GAGGAGCCTGGGAGCATGCTTCAAGCCCTAGAAGAGTTGGAGATCGCAACCCTGAATCAGGCAGATGCGG ATCTGGAGGCTTGTCGAAACCAGATTAGTAAGGACATCATTGCCCTTTTGATGAAGAATCTGGTCAGCGG TGGTCACCTCTCTCCGCAAACAGAAAGGAAGATGGCCGCTGCTTTCAAAAAGCAGTTTCTGCTGTTGGAA AATGAAATACAGGAGGAGTATGAGCGGAAGATGTTGGCACTGACGGCAGAGTGTGACCTAGAGATGAGGA AGAAGACAGAAAACCAGTACCAGAGGGAGATGGTGGCCATGGAGGAAGCAGAAGAGGTGTTGAAGAGAGT TAGCGAGAGGTCTGCTGCCGAGTGCAGCAGCCTTTTGCGGACCCTCCATGGGCTGGAGCAGGAGGACATG CAGAGGTCCCTTACTCTGGACCAAGCCGAGGACTTTGCTCAAGCACACAGGCAGCTGGCTGTTTTCCAGA GGAATGAATTACACAGCATCGTCTATACCCAGATACAGAGTGCTGTCTCCAAAGGGGAACTGAGGCCTGA GGTGGCTAAAATGATGCTGCAAGACTACTCTAAAACACAGGAGAGTGTAGAAGAGCTGATGGACTTCTTC CAGGCCACCAAGCGATACCATCTCAGCAAGAGGTTTGGCCACAGAGAGTACCTGGTCCAGAGACTGCAGG CCATGGAGACCCGGGTGCAAGGGCTGCTGAACACCGCGGCTACCCAGCTAACAAGCCTCATCCATAAACA TGAGAGGGCTGGCTACTTGGATGAAGATCAGATGGAAACGCTATTGGAGCGTGCACAGACAGAAACCTTT TCTATAAAACAGAAGCTGGACAATGACTTAAAGCAGGAAAAGAAAAGGCTCCACCAGAGATTAATAACCA GGAGGCGGCGAGAGCTTCTGCAGAAGCACAAGGAGCAGCAGAAGGAGCAGGTGTCCCTTGGCGAGGCCTC CAGCACCGCGGAGGACGCAGTCCAGTACCTGCACCAGTGGAGGAGCGTGATGGCCGAGCACACCGCTGCC CTGGAGGAGCTGCAGGAACGTCTGGACCAGGCTGCCCTGGATGACCTCCGGGTCCTGACTGTGTCGCTGT CGGAGAAAGCCACGGAAGAGCTGAGGCGCCTGCAGAGCACGGCCATGACACAGGAGCTGCTGAAGCGCAG TGCTCCCTGGCTCTTCCTGCAGCAGATCTTAGAGGAGCACAGCCGGGAGTCTGCGGCTCGCACCACGCAA CTGGAGGCAGAGGAGAGGGAACGTGGCCAGGAGCTGGTGCAGGGCGTGAGGCAGAGGCTACAGCAGGATG CACTGGAGGCCTACACGGAAGAGCAGGCAGAGCTGAGGCACTGGGAGCACTTGGTGTTCATGAAACTCTG CTGTGCGGCCATCTCATTGTCTGAAGAAGACCTGCTCCGGGTAAGGCAGGAGGCACAGGGCTGCTTCAGC CAGCTGGACCGGAGCTTGGCCCTCCCCAGAGTCCGTGCACGAGTGTTGCAGCAGCAGGCGCAGATGGCCT GGCGGGAAGCAGAGTTTCGGAAACTGGACCAGGCCCTGGCTGCGCCTGAGCTGCAGTCCAAGGCAAGAAA GCTGCGTTCCAAGGGCAGAGGCAAGGCCGATCTTCTGAAGAAGAACCTGGAGGATAAGATCCGACTATTC GAGGAGCGGGCCCCTGTAGAACTGGCTGACCAGGTGCGAGGGGAATTGCTCCAAGAGAGGGTCCAGAGGC TGGAGGCCCAGGAGGCGCACTTTGCTGAGTCACTTGTGGCTCTGCAGTTCCAGAAGGTGGCCCGGGCTGC TGAGACCCTCTCAGTCTACACCGCCCTGCTCAGCATTCAAGACCTGCTGCTGGGGGAGCTGAGTGAGTCG GAGACCCTGACCAAGTCAGCCTGTGTGCAGATCCTGGAGTCCCACAGGCCGGAGCTGCAGGAGCTGCAGG AGCTGGAGAGGAAGCTGGAGGACCAGCTGGTGCAGCAGGAAGAGGCCGAGCAGCAGCGGGTCCTGGAGAG CTGGCAGCGGTGGGCGGCCGATGGACCTGGGCTGAGCGAACCTGAGGAGATGGATCCTGAAAGGCAGGTC TCTGCCATCCTGCGGCAAGCCCTGAACAAGGGCCAGAAGCTGCTGGAGCAGCATCAGCAGAGGGTGAGAG AGGAGTGGCAGAACGGTGCGGTGCTGGAAGATTCTCTGGAAAGCATTGAAGCTGACACCATGGCCAGCCT TTGCAGCCAGGGACTGAGGCTGGTGTCATACCTCTCAAGGATGACAATGGTACCAGGCAGCACACTACTG CGACTCCTGAGTGTGGTGCTTCCAGCGGCTTCACAGCCTCAGCTACTGGCCCTGCTGGACGCAGTCAGTG AGAAGCACTCCGACCACACAGCTGAGAATGAGAGCAGTGGTGAGCAGGCTCAGGCAGAGCAGAGCAAGAG GAGGAAACACCAAGTTTGGTGGAAGGTTTTGGACAGCAGATTCAGAGCAGATCTGGTGAGTCAAGGACTG GAGAGGATGCTCTGGGCCCGCCAGAAGAAGGAGCGCATATTGAAGAAGATATATGTTCCCGTCCAGGAGA GGGTGATGTTTCCTGGGAAAGGAAGCTGGCCACACCTGTCCCTAGAACCCATTGGAGAACTGGCTCCCAT CCCCATCACTGGGGCAGACGCTATGGACATATTAAACACGGGAGAAAAGATCTTTGTATTCAGAAGCCCA AGGGAGCCCGAGATCTCTTTGCGTGTTCCTCCCAGGAGGAAAAAGAATTTCCTGAATGCCAAGAAGGCCA ACAGGGCCTTGGGCTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211819 representing NM_145920
Red=Cloning site Green=Tags(s) MGATGPTGAGGRATWVLAGNILAAALVLGSGPRALPPSFPALGPGSPSRPGPAGPWASSQYSDISREARG PFENGVIFQKCSLVSGQSESQTMHVQLSVNNTRTPTSVNLSNLLVLDEITGLAVKESPGNNTQDGIQTFR KSFLQVGECYSVSYTASLDPTALGTGESLDLPARLIFQSPSQNRTQLKAPFTITVEEKIMVLPNHGLHAA GFIAAFLISLLLTVAALFFLARGRCLQGGMLSRCRIQHPENKLEPSPFTSANGVSQDLSLNDQVVAILTS EEPGSMLQALEELEIATLNQADADLEACRNQISKDIIALLMKNLVSGGHLSPQTERKMAAAFKKQFLLLE NEIQEEYERKMLALTAECDLEMRKKTENQYQREMVAMEEAEEVLKRVSERSAAECSSLLRTLHGLEQEDM QRSLTLDQAEDFAQAHRQLAVFQRNELHSIVYTQIQSAVSKGELRPEVAKMMLQDYSKTQESVEELMDFF QATKRYHLSKRFGHREYLVQRLQAMETRVQGLLNTAATQLTSLIHKHERAGYLDEDQMETLLERAQTETF SIKQKLDNDLKQEKKRLHQRLITRRRRELLQKHKEQQKEQVSLGEASSTAEDAVQYLHQWRSVMAEHTAA LEELQERLDQAALDDLRVLTVSLSEKATEELRRLQSTAMTQELLKRSAPWLFLQQILEEHSRESAARTTQ LEAEERERGQELVQGVRQRLQQDALEAYTEEQAELRHWEHLVFMKLCCAAISLSEEDLLRVRQEAQGCFS QLDRSLALPRVRARVLQQQAQMAWREAEFRKLDQALAAPELQSKARKLRSKGRGKADLLKKNLEDKIRLF EERAPVELADQVRGELLQERVQRLEAQEAHFAESLVALQFQKVARAAETLSVYTALLSIQDLLLGELSES ETLTKSACVQILESHRPELQELQELERKLEDQLVQQEEAEQQRVLESWQRWAADGPGLSEPEEMDPERQV SAILRQALNKGQKLLEQHQQRVREEWQNGAVLEDSLESIEADTMASLCSQGLRLVSYLSRMTMVPGSTLL RLLSVVLPAASQPQLLALLDAVSEKHSDHTAENESSGEQAQAEQSKRRKHQVWWKVLDSRFRADLVSQGL ERMLWARQKKERILKKIYVPVQERVMFPGKGSWPHLSLEPIGELAPIPITGADAMDILNTGEKIFVFRSP REPEISLRVPPRRKKNFLNAKKANRALGLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145920 |
| ORF Size | 3660 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145920.3, NP_666032.1 |
| RefSeq Size | 4111 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 68525 |
| UniProt ID | Q8K1G2 |
| MW | 138.1 kDa |
| Gene Summary | Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (PubMed:21356043, PubMed:24582806). Plays a critical role in bone formation and skeletal development (PubMed:21356043). May be involved in early embryonic morphogenesis (PubMed:21356043).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205922 | Evc2 (untagged) - Mouse Ellis van Creveld syndrome 2 homolog (human) (Evc2), (10ug) |
CNY 8,504.00 |
|
| MG211819 | Evc2 (tGFP-tagged) - Mouse Ellis van Creveld syndrome 2 homolog (human) (Evc2) |
CNY 10,550.00 |
|
| MR211819L3 | Lenti ORF clone of Evc2 (Myc-DDK-tagged) - Mouse Ellis van Creveld syndrome 2 homolog (human) (Evc2) |
CNY 11,500.00 |
|
| MR211819L4 | Lenti ORF clone of Evc2 (mGFP-tagged) - Mouse Ellis van Creveld syndrome 2 homolog (human) (Evc2) |
CNY 11,500.00 |
