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Smc4 (BC061481) Mouse Tagged ORF Clone
CAT＃: MR211813
- TrueORF®
Smc4 (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 4 (cDNA clone MGC:70057 IMAGE:6512832)
ORF Plasmid: tGFP
"BC061481" in other vectors (3)
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CNY 8,464.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2500002A22Rik; C79747; SMC-4; Smc4l1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211813 representing BC061481
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTGAAGCTGGAGCTCCTCGTCTCATGATAACTCATATTGTAAACCAAAACTTCAAGTCCTATG CTGGAGAGAAAGTTCTGGGACCTTTCCATAAGCGCTTTTCCTGTATTATTGGACCAAATGGCAGTGGAAA ATCCAATGTTATTGATTCCATGCTTTTTGTGTTTGGCTATCGAGCACAAAAAATAAGGTCTAAAAAGCTA TCGGTACTGATACATAATTCTGATGAACACAAGGATATTCAGAGTTGTACTGTAGAAGTTCATTTTCAAA AGATAATTGATAAGGAAGGAGATGATTACGAAGTCCTTCCTAACAGCAACTTCTATGTATCCAGAACCGC CTACAGAGATAGCACTTCTGTCTACCACATAAGTGGGAAGAAGAAGACGTTTAAAGATGTTGGAAATCTC CTTAGAAGCCATGGCATTGACTTAGACCATAATCGATTTTTAATCTTGCAGGGTGAAGTTGAACAGATTG CTATGATGAAACCCAAAGGCCAGACTGAACATGATGAAGGTATGCTTGAGTATTTAGAAGATATAATTGG CTGTGGACGGCTAAATGAACCTATTAAAGTCTTGTGTCGGAGAGTTGAAATATTAAATGAACACAGAGGA GAAAAGTTAAATAGAGTGAAAATGGTAGAAAAGGAGAAGGATGCACTAGAGGGAGAGAAAAACATAGCCA TTGAGTTCCTTACCCTGGAAAATGAAATGTTTAAGAAAAAGAATCACATCTGTCAATACTACATTTATGA TCTGCAGAATAGAATTGCTGAAATAACGACTCAAAAGGAAAAAATTCATGAAGATACTAAAGAAATTACT GAGAAGAGCAATGTGCTTTCCAATGAAATGAAAGCGAAGAATTCAGCTGTAAAGGATGTAGAAAAGAAGC TCAATAAAGTTACAAAATTTATCGAACAGAATAAAGAAAAATTTACACAACTAGATTTGGAAGATGTTCA AGTTAGGGAAAAATTAAAACATGCTACAAGTAAAGCCAAGAAACTGGAGAAACAACTTCAAAAAGATAAA GAAAAGGTTGAAGAGCTGAAAAGTGTACCTGCCAAGAGTAAGACTGTCATAAATGAGACAACAACTAGAA ACAATTCGCTTGAGAAAGAAAGAGAGAAAGAAGAAAAAAAATTAAAAGAGGTCATGGACAGCCTTAAGCA GGAAACACAGGGGCTTCAGAAAGAAAAGGAGATTCAAGAAAAGGAACTTATGGGTTTTAACAAATCAGTA AATGAAGCACGTTCAAAGATGGAAGTTGCTCAGTCAGAACTTGATATCTATCTCAGTCGACATAATACAG CAGTGTCTCAATTAAGTAAGGCGAAGGAAGCCTTAATTACAGCTTCTGAGACTCTCAAGGAGAGGAAAGC TGCAATCAAGGATATAAACACAAAGCTCCCTCAGACGCAACAGGAACTAAAGGAGAAAGAAAAAGAACTT CAGAAACTGACACAAGAAGAAATAAACTTAAAAAGTTTGGTTCATGATCTTTTCCAAAAAGTTGAAGAAG CAAAGAGTTCCTTAGCAATGAATCGAAGTAGGGGGAAAGTACTTGATGCAATAATTCAAGAAAAAAAATC AGGCAGGATCCCAGGAATATATGGAAGATTGGGAGACTTAGGAGCTATTGATGAAAAATATGACATTGCT ATTTCATCCTGTTGCCATGCATTGGACTACATTGTTGTTGATTCTATTGATACAGCCCAAGAATGTGTAA ACTTCCTTAAAAAGCATAATATTGGGATTGCAACTTTCATAGGTTTGGATAAGATGACAGTGTGGGCCAA AAAAATGAGCAAAATTCAAACTCCTGAAAATACTCCTCGGTTATTTGATCTAGTTAAAGTGAAAAATGAG GAAATCCGCCAAGCCTTTTACTTTGCTTTACGAGATACCTTGGTGGCTAACAATTTGGATCAAGCTACAA GAGTAGCGTACCAAAGAGACAGACGCTGGAGAGTAGTGACTCTGCAGGGACAGATCATAGAACAGTCAGG TACAATGAGTGGCGGTGGAAGCAAGGTGATGAGAGGAAGGATGGGCTCTTCGGTTATCGATGAAATCTCT GTAGAAGAGGTAAATAAGATGGAATCCCAGTTGGAAAGGCATTCTAAACAAGCAATGCAAATCCAAGAAC AGAAAGTACAACATGAAGAAGCAGTAGTTAAGTTACGGCACAGTGAACGAGACATGAGAAATACACTGGA AAAGTTCGCTGCAAGCATCCAGGGCTTATCAGAGCAAGAAGAGTATCTGTGTGTACAAATTAAAGAACTG GAAGCTAACGTTCTTACTACGGCCCCTGACAGAAAACAACAGAAACTGCTGGAAGAAAATGTCAGTGTTT TCAAAAAAGAATATGATGCTGTGGCTGAGAAAGCTGGTAAAGTTGAAGCTGAGATTAAACGGCTACACAA TACCATCATAGATATCAACAACCGAAAACTCAAGGCCCAGCAAAACAAACTTGACACGATAAATAAGCAG CTTGATGAATGTGCTTCTGCTATTACTAAGGCCCAAGTAGCAATCAAGACTGCTGACAGAAATCTTAAAA AGGCACAGGATTCTGTTTGCCGCACAGAGAAAGAAATAAAAGACACTGAAAAAGAAATAAATGACTTAAA GACAGAGCTAAAAAATATTGAAGACAAGGCAGAAGAGGTCATAAACAATACAAAAACTGCAGAGACATCC TTACCAGAAATCCAGAAAGAACATCGTAATCTACTTCAGGAACTAAAAGTTATTCAAGAAAATGAACATG CTCTTCAGAAAGATGCACTTAGTATTAAGTTGAAACTTGAACAGATAGATGGCCACATTTCTGAACATAA CTCAAAAATAAAATACTGGCAAAAAGAGATTTCAAAAATAAAATTGCATCCTGTTGAAGATAATCCTGTT GAGACAGTAGCAGTTCTAAGCCAGGAGGAGCTTGAGGCAATCAAGAATCCAGAGTCTATAACAAATGAAA TTGCACTTTTGGAAGCTCAGTGTCGTGAAATGAAACCAAACCTTGGAGCCATCGCTGAGTATAAAAAAAA GGAAGATTTATATTTGCAAAGAGTAGCCGAACTGGACAAAATTACTTCTGAAAGAGATAATTTTAGACAA GCATATGAAGATCTTCGAAAACAAAGGCTGAATGAATTTATGGCTGGTTTTTACGTAATAACAAATAAAC TAAAAGAAAACTACCAGATGCTCACATTGGGAGGAGATGCTGAACTGGAGCTTGTGGACAGTTTAGATCC TTTTTCTGAAGGAATCATGTTCAGTGTTCGGCCACCTAAGAAAAGTTGGAAGAAGATCTTTAACCTCTCA GGAGGCGAGAAAACCCTTAGTTCCTTGGCCTTAGTGTTTGCTCTTCATCACTACAAGCCCACTCCCCTCT ACTTCATGGATGAGATTGATGCAGCTCTGGATTTTAAAAATGTGTCTATTGTTGCATTTTATATATATGA GCAAACAAAAAATGCCCAGTTCATAATAATCTCTCTTCGAAATAATATGTTTGAGATTTCAGACAGACTT ATTGGCATCTATAAAACATATAATAGTACAAAAAGTGTTGCAGTGAACCCAAAACAAATTGCGTCTAAAG GACTCTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211813 representing BC061481
Red=Cloning site Green=Tags(s) MASEAGAPRLMITHIVNQNFKSYAGEKVLGPFHKRFSCIIGPNGSGKSNVIDSMLFVFGYRAQKIRSKKL SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVLPNSNFYVSRTAYRDSTSVYHISGKKKTFKDVGNL LRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRG EKLNRVKMVEKEKDALEGEKNIAIEFLTLENEMFKKKNHICQYYIYDLQNRIAEITTQKEKIHEDTKEIT EKSNVLSNEMKAKNSAVKDVEKKLNKVTKFIEQNKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDK EKVEELKSVPAKSKTVINETTTRNNSLEKEREKEEKKLKEVMDSLKQETQGLQKEKEIQEKELMGFNKSV NEARSKMEVAQSELDIYLSRHNTAVSQLSKAKEALITASETLKERKAAIKDINTKLPQTQQELKEKEKEL QKLTQEEINLKSLVHDLFQKVEEAKSSLAMNRSRGKVLDAIIQEKKSGRIPGIYGRLGDLGAIDEKYDIA ISSCCHALDYIVVDSIDTAQECVNFLKKHNIGIATFIGLDKMTVWAKKMSKIQTPENTPRLFDLVKVKNE EIRQAFYFALRDTLVANNLDQATRVAYQRDRRWRVVTLQGQIIEQSGTMSGGGSKVMRGRMGSSVIDEIS VEEVNKMESQLERHSKQAMQIQEQKVQHEEAVVKLRHSERDMRNTLEKFAASIQGLSEQEEYLCVQIKEL EANVLTTAPDRKQQKLLEENVSVFKKEYDAVAEKAGKVEAEIKRLHNTIIDINNRKLKAQQNKLDTINKQ LDECASAITKAQVAIKTADRNLKKAQDSVCRTEKEIKDTEKEINDLKTELKNIEDKAEEVINNTKTAETS LPEIQKEHRNLLQELKVIQENEHALQKDALSIKLKLEQIDGHISEHNSKIKYWQKEISKIKLHPVEDNPV ETVAVLSQEELEAIKNPESITNEIALLEAQCREMKPNLGAIAEYKKKEDLYLQRVAELDKITSERDNFRQ AYEDLRKQRLNEFMAGFYVITNKLKENYQMLTLGGDAELELVDSLDPFSEGIMFSVRPPKKSWKKIFNLS GGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISDRL IGIYKTYNSTKSVAVNPKQIASKGLC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC061481 |
| ORF Size | 3648 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC061481.1 |
| RefSeq Size | 4097 bp |
| RefSeq ORF | 3650 bp |
| Locus ID | 70099 |
| MW | 150.2 kDa |
| Gene Summary | Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211813 | Smc4 (tGFP-tagged) - Mouse structural maintenance of chromosomes 4 (cDNA clone MGC:70057 IMAGE:6512832) |
CNY 7,600.00 |
|
| MR211813L3 | Lenti ORF clone of Smc4 (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 4 (cDNA clone MGC:70057 IMAGE:6512832) |
CNY 8,840.00 |
|
| MR211813L4 | Lenti ORF clone of Smc4 (mGFP-tagged) - Mouse structural maintenance of chromosomes 4 (cDNA clone MGC:70057 IMAGE:6512832) |
CNY 8,840.00 |
