United States
Germany
Japan
United Kingdom
China
Tonsl (NM_183091) Mouse Tagged ORF Clone
CAT＃: MR211809
- TrueORF®
Tonsl (Myc-DDK-tagged) - Mouse tonsoku-like, DNA repair protein (Tonsl)
ORF Plasmid: tGFP
"NM_183091" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,456.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810439M11Rik; Nfkbil2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211809 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGACCCAGCGGGAACTGAGTGAGATGAGGACCCGTCTCTACCTCAATCTGGGTCTCACCTGTGAGA GCCTGCAACAGACAGCCCTGTGCAACAACTACTTCAAAAAGAGCATCTTTCTTGCTGAGCAGAACCATCT CTATGAAGATCTGTTCCGGGCCCGATACAACCTGGGTGCCATCCACTGGCGCGGAGGGCAGCACTCTCAG GCCATGCGCTGCTTAGAAGGGGCCCGGGAATGTGCCCGTGCTATGAAGATGAGATTCATGGAGAGTGAAT GTTGCGTGCTAGTGTCCCAGGTGCTCCAAGATCTGGGGGACTTCTTAGCTGCCAAACGAGCCCTGAAGAA GGCCTATAGGTTGGGCTCTCAGAAGCCTAACCAGAGAGTGACTGTCTGTCAGAGTCTCAAGTATGTATTG GCAGTGATCCAGCTGCAGCAGCAGCTGGAAGAGGCTGAGGGCAATGATCTTCAAGGTGCCATGGCTATCT GTGAACAGTTGGGGGACCTGTTCTCCAAAGCGGGTGACTTTCCCAAGGCAGCTGAGGCTTACCAGAAGCA GCTGCACTTGGCTGAGCTGCTGAACAGACCAGATCTGGAGCTGGCTGTTATCCACGTATCCCTGGCCACC ACACTGGGAGACATGAAGGATCACCGCAAGGCTGTGCACCACTATGAGGAAGAACTGAGGCTGCGCAAGG GCAACGCTCTGGAGGAAGCTAAGACTTGGTTCAACATTGCCCTGTCACGTGAGGAGGCTGGAGACGCATA CGAGCTGCTAGCTCCATGCTTCCAGAAGGCTTTTTGCTGTGCCCAGCAGGCCCAGAGATTCCAGCTGCAG AGGCAGATCTTACAGCACCTTTATACCGTGCAACTAAAGTTGCAGCCACAAGAAGCCCGTGACACTGAAA TCAGACTGCAGGAACTGAGTATGGCAAAAGACACAGAAGAAGAGGAGGAGGAAGAGGAGGAAGAAGAGGA AGAAGCCAGTGAGGCCCCAGAGACCAGTGAACTGGAACTCTCAGAGAGCGAGGATGATGCTGACGGCCTG TCTCAGCAGCTGGAAGAAGATGAGGAGCTTCAGGGCTGTGTGGGCCGGCGGAAGGTGAACAAGTGGAACC GGCGCAATGACATGGGAGAGACCCTGCTGCACCGAGCTTGCATTGAAGGCCAACTGCGTCGTGTCCAGGA TCTTGTGAAGCAGGGCCATCCCCTGAATCCCCGAGACTACTGCGGCTGGACACCTCTCCATGAAGCATGC AACTATGGACATCTTGAGATTGTTCGCTTCCTTCTGGACCATGGAGCAGCAGTGGATGACCCAGGTGGCC AGGGGTGTGATGGCATCACCCCCCTGCATGACGCCCTCAACTGTGGCCACTTTGAGGTAGCTGAATTACT CATTGAGCGAGGGGCATCCGTGACTCTCCGTACCAGGAAGGGCCTCAGCCCACTGGAGACACTGCAGCAG TGGGTGAAGTTGTACTTCAGGGACCTTGACCTTGAGACAAGACAGAAGGCGGCAACCATGGAGGAGAGGC TCCAGATGGCCTCCTCAGGCCAAGCTTCCCGCAGCTCCCCTGCCCTCCAGACCATTCCAAGTAACCATCT ATTTGACCCTGAGACCTCTCCTCCCTCAAGCCCCTGTCCAGAACCCTCCTCATATACTCCTAGACCTCCA GAGGCCTCTCCGGCCCCTGCTAAAGTCTTTCTGGAGGAAACTGTGTCTGCCGTGTCCCGACCTCGAAAGA CCAGGCACAGACCAACCAGCAGCAGTAGCAGCTCAGAGGATGAGGATAACCCAAGTCCCTGCAGGCCATC GCAGAAAAGACTGAGACATACCACACAGCAGGGTGAAGTCAAGATTCCTGACCCACCCAAAAGTAGAGAG ACAGCCACGTCAAGTGCTTGCCGGGCGGCTTACCAAGCGGCCATCCGAGGCGTGGGTAGTGCCCAGAGCC GTCGCTTGGTGCCTAGCCTGCCTCGGGGCTCAGAGGAAGTCCCTGCCCCCAAGACAGCACTCATTCCCGA GGAGGAATACCTGGCTGGGGAATGGCTGGAGGTAGATACACCTCTGACCCGCAGCGGCAGGCCCAGCACC TCGGTGTCAGACTATGAGCGATGTCCTGCAAGGCCCCGGACCCGTGTCAAGCAGAGTCGCCTGACAAGTC TTGATGGTTGGTGTGCACGGACTCAAGCAGGAGATGGCAGCTTGAATGCAGAGCCTGCAGAGAACCCCAG CGTGCCTAGGACCTCAGGACCCAACAAGGAAAATTATGCAGCAGGCCAGCCTTTGCTTCTGGTCCAGCCC CCTCCCATCCGGGTTCGAGTTCAAATTCAGGATAACCTTTTCCTCATCCCCGTTCCCCAGAGTGACATCC GCCCCGTGGCTTGGCTCACAGAGCAAGCCGCCCAGCGCTATTTCCAAACGTGTGGGCTGCTCCCAAGGCT CACCCTGCGGAAGGATGGCGCACTGCTGGCCCCGCAGGACCCCATCCCTGACGTGCTGCAGAGCAACGAT GAGGTGTTAGCTGAGGTAACTTCGTGGGACCTTCCTCCGCTAAAGGACCGCTACCGCAGGGCCTGCCTGA GCCTGGGGCAAGGGGAGCACCAGCAAGTGCTGCATGCCATGGACCACCAGAGTTCCAGCCCTTCGTTCAG TGCCTGCTCCCTAGCCTTGTGCCAAGCCCAGCTCACACCCCTGCTGAGGGCCCTCAAGTTACACACAGCA CTCCGGGAGCTACGCCTTGCAGGGAACCGACTAGGTGATGCCTGCGCTACCGAGCTGCTGGCCACCCTGG GCACCACGCCTAACCTGGTCCTCCTCGATCTCTCTTCCAATCACTTGGGCCAGGAAGGTCTGCGTCAGCT TGTGGAAGGCTCCTCGGGCCAGGCTGCTTTGCAGAACTTGGAGGAGCTGGACTTGAGTATGAACCCACTA GGAGATGGCTGTGGTCAGGCCCTGGCCTCCCTTCTGCGGGCCTGCCCCATGCTCAGCACCCTGCGCCTAC AGGCCTGTGGCTTCAGCTCCAGTTTCTTCCTGAGCCACCAGGCTGCCCTGGGCGGTGCCTTCCAAGATGC TGTGCACCTGAAAACCTTGTCCTTATCGTACAATCTGCTCGGCGCTCCTGCCCTGGCGAGGGTGCTGCAG ACCTTACCCGCCTGTACCCTCAAACGTCTGGACCTAAGCTCTGTGGCAGCCAGCAAGAGCAACTCGGGTA TCATAGAGCCTGTTATCAAATACTTGACCAAGGAAGGCTGTGCTCTGGCCCACCTAACCCTGTCCGCAAA CTGCCTGGGTGACAAGGCCGTGAGAGAACTGAGCAGATGTCTCCCTTGCTGCCCCTCACTCACCTCTCTG GACCTGTCTGCCAACCCCGAGGTCAGCTGTGCCAGTCTAGAGGAGCTCTTGTCTGCCCTCCAAGAGCGGT CCCAAGGCCTCAGCTTCCTTGGCCTATCAGGCTGCTCTATTCAAGGGCCGCTGAACTCTGACCTCTGGGA CAAGATCTTCGTGCAGTTGCAGGAGTTGCAACTGTGCACCAAAGACCTGAGCACCAAAGACCGAGACTCG GTGTGTCAGAGACTGCCAGAGGGTGCGTGCACAATGGACCAAAGTTCCAAGCTCTTCTTTAAATGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211809 protein sequence
Red=Cloning site Green=Tags(s) MLTQRELSEMRTRLYLNLGLTCESLQQTALCNNYFKKSIFLAEQNHLYEDLFRARYNLGAIHWRGGQHSQ AMRCLEGARECARAMKMRFMESECCVLVSQVLQDLGDFLAAKRALKKAYRLGSQKPNQRVTVCQSLKYVL AVIQLQQQLEEAEGNDLQGAMAICEQLGDLFSKAGDFPKAAEAYQKQLHLAELLNRPDLELAVIHVSLAT TLGDMKDHRKAVHHYEEELRLRKGNALEEAKTWFNIALSREEAGDAYELLAPCFQKAFCCAQQAQRFQLQ RQILQHLYTVQLKLQPQEARDTEIRLQELSMAKDTEEEEEEEEEEEEEASEAPETSELELSESEDDADGL SQQLEEDEELQGCVGRRKVNKWNRRNDMGETLLHRACIEGQLRRVQDLVKQGHPLNPRDYCGWTPLHEAC NYGHLEIVRFLLDHGAAVDDPGGQGCDGITPLHDALNCGHFEVAELLIERGASVTLRTRKGLSPLETLQQ WVKLYFRDLDLETRQKAATMEERLQMASSGQASRSSPALQTIPSNHLFDPETSPPSSPCPEPSSYTPRPP EASPAPAKVFLEETVSAVSRPRKTRHRPTSSSSSSEDEDNPSPCRPSQKRLRHTTQQGEVKIPDPPKSRE TATSSACRAAYQAAIRGVGSAQSRRLVPSLPRGSEEVPAPKTALIPEEEYLAGEWLEVDTPLTRSGRPST SVSDYERCPARPRTRVKQSRLTSLDGWCARTQAGDGSLNAEPAENPSVPRTSGPNKENYAAGQPLLLVQP PPIRVRVQIQDNLFLIPVPQSDIRPVAWLTEQAAQRYFQTCGLLPRLTLRKDGALLAPQDPIPDVLQSND EVLAEVTSWDLPPLKDRYRRACLSLGQGEHQQVLHAMDHQSSSPSFSACSLALCQAQLTPLLRALKLHTA LRELRLAGNRLGDACATELLATLGTTPNLVLLDLSSNHLGQEGLRQLVEGSSGQAALQNLEELDLSMNPL GDGCGQALASLLRACPMLSTLRLQACGFSSSFFLSHQAALGGAFQDAVHLKTLSLSYNLLGAPALARVLQ TLPACTLKRLDLSSVAASKSNSGIIEPVIKYLTKEGCALAHLTLSANCLGDKAVRELSRCLPCCPSLTSL DLSANPEVSCASLEELLSALQERSQGLSFLGLSGCSIQGPLNSDLWDKIFVQLQELQLCTKDLSTKDRDS VCQRLPEGACTMDQSSKLFFKCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183091 |
| ORF Size | 3642 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183091.2, NP_898914.2 |
| RefSeq Size | 4239 bp |
| RefSeq ORF | 4092 bp |
| Locus ID | 72749 |
| UniProt ID | Q6NZL6 |
| MW | 134.1 kDa |
| Gene Summary | Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205306 | Tonsl (untagged) - Mouse tonsoku-like, DNA repair protein (Tonsl), (10ug) |
CNY 8,456.00 |
|
| MG211809 | Tonsl (tGFP-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 (Nfkbil2) |
CNY 11,880.00 |
|
| MR211809L3 | Lenti ORF clone of Tonsl (Myc-DDK-tagged) - Mouse tonsoku-like, DNA repair protein (Tonsl) |
CNY 12,730.00 |
|
| MR211809L4 | Lenti ORF clone of Tonsl (mGFP-tagged) - Mouse tonsoku-like, DNA repair protein (Tonsl) |
CNY 12,730.00 |
