Ankrd15 (BC079563) Mouse Tagged ORF Clone

CAT#: MR211799

  • TrueORF®

Ankrd15 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 15 (cDNA clone MGC:90826 IMAGE:30535512)

ORF Plasmid: DDK tGFP



  "BC079563" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,368.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms mKIAA0172, A930031B09Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211799 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACCCGGAGAAGACTGGAACAGGAGCGAGTCACCATGCAGATGGCACCGGGTGACTTCAGGAGGC
CCAGGCTGGCCAGTTTTGGAGGCATGGGCTCCACAAGCTCCCTCCCGTCCTTTGTGGGGTCTGCTAACCA
CAGTTCTGTAATACACCAGCTTCAGAATGGCTACCAAGGCAATGGGGATTATAACAGCTACGTCCCAGCA
GCTCCTACCACGTCTTCCATGGGAAGCTCCGTCCGGCACAGCCCATTGAGTTCAGGGATCTCCACCCCAG
TGACCAACGTGAGCCCCATGCACCTGCAGCACATCCGAGAGCAGATGGCCATCGCCTTAAAACGCCTGAA
GGAGCTTGAGGAGCAGGTGAGGACCATCCCTGTGCTCCAGGTCAAGATCTCCGTCTTGCAAGAAGAGAAA
AGGCAGTTGGCCTCGCAGCTGAAAAGCCAGAGGGCCTCGTCCCAGAACGAAGCGTGCGGTGTGAGGAAAC
GCTCCTACAGTGCGGGCAACGCCTCCCAGCTGGAACTGCTTGCCCGAGCCCGAAGAGGCGGCGGGGAATT
GTACATCGACTACGAGGAGGAAGAGATGGAGAGCGTAGAGCAGAGCACGCAGCGGATCCAGGAGTTCCGA
CAGCTCACGGCCGACATGCAGGCGCTGGAGCGGAAGATTCAGGATAGCAGCTGCGAGGTGGCATCAGAGC
TCAGGGAGAATGGGCAGTGCCCGTCTCGGGAGTGCAAGTCTGTGGCCGTGGGTAGTGATGAGAACATGAA
CGATGTCGTTGTATACCACAGAGACTTAAGGCCCTGCAAGGATACGGCTGTGGGGACGGTCACCGAGACG
AGGAATGTTGGCATCAGCGTGACAGAAGCTATGCTTGGGGTTATCACTGAAGCTGACAAAGAGATTGAGC
TGCAGCAGCAGACCATAGAGGCCTTGAAGGAGAAGATTTACCGCCTAGAAGTACAGCTTAAAGAAACCAC
CCATGACCGCGAGATGACTAAGCTCAAGCAAGAACTACAGGCTGCCGGATCCAGGAAGAAAGTTGACAAG
GCCACAATGGCCCAGCCACTTGTCTTCAGCAAGTTGGTGGAGGCACTGGTGCCAACCAGAGATCAAATGG
TTGGCAGTCATGTGGACACAAGGGAGTCATGTGTTGGGACCTCTGTGCAAACTAGTAGCGTAGGCACCTC
CTGCCATCCTGACTGCAAGAACCAAGTCGTGGGATCCGAGCTGCCCATGAATTGGTGGGTCGTGAAGGAG
AGGGTGGCAATGCATGACCAATGCGTAGGGAGGTCTGTTGAGACTTGTGACCGGAGTGTGGGTGTGGAAG
TCAGTGTCTGTGAAACAGGCAGCAACACAGAGGCTTCTGGGAGCGACCTGACACTCCTTAAGACAAACTT
GAACCTCAAAGACGTGCGGTCCATCGGCTGTGGAGATTGTTCCGTGGATGTGATTGTCTGCTTCCCCAAG
GAGTGCACCTCCCGAAGCATGAACACGGAGGCTGTAGGCCAGGAGGAAGCAGCTGTCATGGCGGTGCCCC
ATACCACAGACCAGCACACCAGCACGAATCTGGAGCGGGTGGACCAGTGCACTAACACAGAGGCAGCCAC
CCTGGTGGAGTCCTGCACCAACACTCTCCTCAGCACTATGGACAAGCAGACCAGCACCCAGACTGTGGAG
ATGCGAACGGTGGCCATCGGAGAAGGCCGCGTCAGAGACATCAACCCCTCCACTAAGACTCGGTCCGTTG
GTGTCGGGACAGTGCTGTCTGGCAATTCTGAGTTTGACAGGCCATGTGCTGTGAAGACCAAAGAGTCGGG
TGTGGGACAGATAAATATTCAGGACAACTATCTAGTTGGTCTCAAAATGCGGACCATAGCGTGCGGGCCT
CCACAGTTGACTGTGGGGCTGATGGGCAGCAGGAGGAGCGTGGGTGTTGGGAACGAGCCCGTAGGAGAGC
TCCCGGAGGAGTCTCCTCAGCCTCGGGTGGCGTCTGGAATGGTGACTGGCTTGGATCACTACATTGAGCG
CGTGCAGAGGCTGCTGGCAGAGCAACAGACGTTGCTGGCTGAGAACTACAGTGAGCTGGCGGAGGCTTTT
GGGGAGCCTCATTCACAGATTGGCTCCCTCAACTCGCAGCTCATCAGCACCCTGTCGTCCATCAATTCCG
TCATGAAGTCTGCAAGCACCGAGGAGCTCAGGAACTCTGACTTCCAGAAAGCCAGTCTGGGTAAAACTAC
AGGAAATCACTTGGAATATACCTGCAAGTGTGGAGGCCTTCGGTCAGGAGGACTGTTAAATGTACAACCA
TCCCAGCCTGAGGTGGAGGCGGAGACTGCAGAAGGCAAGCACAGCCGGGGCCATGAGCAGTTCCCCATGC
AAGGAAGTACCCTGCCCCCGGTGAACCTGACAGATGACCAGATAGCCACTGGCCTCTACGTATGTCCAAA
TAATGAAAACACACTGAAGTCCATCATGAAGAAAAGTGATGGGAATAAAGATTCAAATGGAGCCAAAAAG
AATCTTCAGTTCATTGGCATTAACGGCGGGTATGAGACAACTTCAAGTGACGAATCCAGCTCGGATGGAA
GCTCTTCTTCCGAGTCAGATGACGAGTGTGACACCATTGGGTACCCTCCCGAGGAAGAGGAGGAGGAGGA
AGAGAAGGACCACGACACTCGGGGAATGGCGGAAGGGCACCACGCAGTTAATATTGAAGGTTTCAAGTCT
GCCAGGGTGGAAGATGAAGTGCAGGTTCCAGAATGTGAGCCTGAGAAGGAGGAAATCAGAGAGAGGTATG
AACTAAGTGAAAAGATGTTGTCAGCCTGCAACTTACTGAAATACAACATAAAGGATCCCAAAGCTTTGGC
CAGCAAAGATATGAGGATCTGTCTGAACACCCTCCAGCACGATTGGTTCCGAGTGTCCAGTCAGAAGTCC
GCCGTGCCAGCCATGGTGGGCGACTACATCGCCGCGTTCGAGGCTGTCTCCCCGGACGTGCTCCGGTACA
TCATCAACATGGCGGACGGCAATGGCAACACTGCCCTCCACTACAGCGTGTCCCACTCCAACTTCCAGAT
CGTCAAGCTGCTTCTGGATGCAGACGTGTGCAATGTGGATCACCAGAACAAGGCAGGGTACACACCCATC
ATGCTGGCAGCCCTCGCGGCCGTGGAGGCAGAGAAGGACATGCAGGTCGTAGAAGAACTCTTCAGCTGTG
GAGACGTGAACGCCAAGGCCAGTCAGGCGGGACAGACGGCCCTCATGCTAGCTGTCAGTCACGGGCGGAT
AGACATGGTGAAGGGCCTGCTGGCCTGTGGAGCTGATGTCAATATCCAGGACGACGAGGGCTCCACCGCC
CTGATGTGTGCCAGTGAACACGGGCATGTGGAGATTGTGAAGCTGCTGCTGGCCCAGCCAGGCTGCAATG
GCCACCTGGAAGACAATGATGGCAGCACCGCTCTCTCCATAGCCCTGGAAGCTGGACACAAGGACATTGC
CGTTCTTCTGTACGCCCACCTCAACTTCTCGAAAGCCCAGTCCCCGAGCACCCCGAGGCTTGGCAGAAAG
ACATCTCCTGGTCCCACTCACCGAGGTTCTTTTGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211799 protein sequence
Red=Cloning site Green=Tags(s)

METRRRLEQERVTMQMAPGDFRRPRLASFGGMGSTSSLPSFVGSANHSSVIHQLQNGYQGNGDYNSYVPA
APTTSSMGSSVRHSPLSSGISTPVTNVSPMHLQHIREQMAIALKRLKELEEQVRTIPVLQVKISVLQEEK
RQLASQLKSQRASSQNEACGVRKRSYSAGNASQLELLARARRGGGELYIDYEEEEMESVEQSTQRIQEFR
QLTADMQALERKIQDSSCEVASELRENGQCPSRECKSVAVGSDENMNDVVVYHRDLRPCKDTAVGTVTET
RNVGISVTEAMLGVITEADKEIELQQQTIEALKEKIYRLEVQLKETTHDREMTKLKQELQAAGSRKKVDK
ATMAQPLVFSKLVEALVPTRDQMVGSHVDTRESCVGTSVQTSSVGTSCHPDCKNQVVGSELPMNWWVVKE
RVAMHDQCVGRSVETCDRSVGVEVSVCETGSNTEASGSDLTLLKTNLNLKDVRSIGCGDCSVDVIVCFPK
ECTSRSMNTEAVGQEEAAVMAVPHTTDQHTSTNLERVDQCTNTEAATLVESCTNTLLSTMDKQTSTQTVE
MRTVAIGEGRVRDINPSTKTRSVGVGTVLSGNSEFDRPCAVKTKESGVGQINIQDNYLVGLKMRTIACGP
PQLTVGLMGSRRSVGVGNEPVGELPEESPQPRVASGMVTGLDHYIERVQRLLAEQQTLLAENYSELAEAF
GEPHSQIGSLNSQLISTLSSINSVMKSASTEELRNSDFQKASLGKTTGNHLEYTCKCGGLRSGGLLNVQP
SQPEVEAETAEGKHSRGHEQFPMQGSTLPPVNLTDDQIATGLYVCPNNENTLKSIMKKSDGNKDSNGAKK
NLQFIGINGGYETTSSDESSSDGSSSSESDDECDTIGYPPEEEEEEEEKDHDTRGMAEGHHAVNIEGFKS
ARVEDEVQVPECEPEKEEIRERYELSEKMLSACNLLKYNIKDPKALASKDMRICLNTLQHDWFRVSSQKS
AVPAMVGDYIAAFEAVSPDVLRYIINMADGNGNTALHYSVSHSNFQIVKLLLDADVCNVDHQNKAGYTPI
MLAALAAVEAEKDMQVVEELFSCGDVNAKASQAGQTALMLAVSHGRIDMVKGLLACGADVNIQDDEGSTA
LMCASEHGHVEIVKLLLAQPGCNGHLEDNDGSTALSIALEAGHKDIAVLLYAHLNFSKAQSPSTPRLGRK
TSPGPTHRGSFD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC079563
ORF Size 3606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC079563, AAH79563
RefSeq Size 4752 bp
RefSeq ORF 3608 bp
Locus ID 107351
MW 130.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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