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Sin3a (BC052716) Mouse Tagged ORF Clone
CAT＃: MR211790
- TrueORF®
Sin3a (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3A (yeast) (cDNA clone MGC:64685 IMAGE:6837144)
ORF Plasmid: tGFP
"BC052716" in other vectors (4)
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CNY 8,336.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW553200; mKIAA4126; mSin3A; Sin3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211790 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGACGTTTGGATGACCAGGAATCACCAGTGTATGCAGCCCAGCAGCGAAGGATTCCTGGGAGCA CAGAGGCTTTTTCTCACCAGCACCGGGTCCTTGCCCCGGCCCCTCCTGTGTATGAAGCAGTGTCTGAGAC CATGCAGTCAGCTACAGGCATTCAGTACTCAGTGGCACCCAACTACCAGGTTTCAGCTGTGCCACAAAGT TCTGGCAGTCATGGGCCCGCCATAGCAGCAGTTCATAGCAGCCATCATCACCCAACAGCTGTCCAGCCTC ATGGAGGCCAGGTGGTCCAGAGCCATGCCCACCCAGCACCACCAGTTGCACCAGTACAGGGACAGCAGCA GTTTCAGAGGCTCAAGGTGGAAGACGCCCTGTCCTATCTTGACCAGGTGAAACTGCAGTTCGGTAGTCAG CCTCAGGTCTACAATGATTTCCTTGACATCATGAAGGAATTTAAATCTCAGAGCATTGATACTCCAGGAG TGATTAGCCGAGTGTCCCAGCTATTTAAAGGCCACCCTGATCTGATCATGGGCTTTAACACCTTCTTGCC TCCTGGCTACAAAATTGAGGTGCAGACTAATGACATGGTGAACGTGACAACACCTGGCCAAGTTCATCAG ATTCCCACCCATGGCATCCAGCCCCAGCCTCAGCCACCACCTCAGCATCCTTCCCAGCCTTCATCCCAGT CAGCTCCCACTCCTGCTCAGCCAGCTCCTCAGCCCACAGCTGCCAAAGTCAGCAAGCCTTCCCAACTACA AGCACATACTCCAGCCAGTCAGCAGACTCCCCCACTCCCACCATATGCATCCCCACGTTCTCCACCAGTC CAGCCTCACACACCAGTGACAATCTCCTTGGGGACAGCTCCATCTTTGCAAAACAATCAGCCTGTGGAGT TTAATCATGCCATCAACTATGTTAATAAGATCAAGAACAGATTCCAGGGCCAACCAGACATCTACAAAGC ATTCTTGGAGATTTTGCACACATACCAGAAAGAACAGCGGAATGCCAAGGAAGCTGGAGGAAACTACACT CCAGCTTTGACTGAGCAAGAGGTGTATGCCCAGGTGGCTCGACTCTTCAAAAACCAGGAAGATTTGTTGT CTGAATTTGGACAGTTCCTGCCAGATGCCAACAGCTCAGTGCTTTTAAGCAAAACAACTGCTGAGAAGGT TGATTCTGTGAGAAATGACCATGGAGGCACTGTGAAGAAGCCCCAACTGAATAACAAGCCACAGAGGCCC AGTCAGAATGGCTGCCAGATCCGCAGGCACTCTGGAACAGGAGCCACACCTCCAGTGAAGAAAAAACCCA AACTGATGAGTCTAAAAGAGTCTTCAATGGCAGATGCCAGCAAGCATGGTGTTGGAACGGAATCATTATT TTTTGATAAGGTTCGAAAGGCTCTTCGGAGTGCAGAGGCCTATGAAAACTTCCTTCGTTGCCTTGTTATC TTTAATCAGGAGGTGATCTCTCGGGCCGAGCTTGTACAGCTAGTCTCTCCTTTTCTGGGGAAATTCCCTG AATTGTTTAATTGGTTTAAAAACTTTTTGGGCTATAAGGAGTCTGTACATCTGGAAAGCTTTCCAAAGGA ACGAGCTACAGAAGGCATTGCCATGGAGATAGACTATGCCTCTTGTAAACGACTGGGCTCTAGCTACCGA GCCCTACCGAAAAGTTACCAGCAGCCCAAGTGCACGGGACGGACTCCTCTGTGTAAAGAGGTTTTAAATG ATACCTGGGTTTCCTTCCCATCTTGGTCTGAAGACTCCACTTTTGTTAGTTCCAAGAAGACTCAGTATGA AGAACATATTTACCGTTGTGAAGATGAACGATTTGAGCTTGATGTGGTTCTTGAGACCAATCTTGCAACA ATCCGGGTTTTAGAAGCAATACAGAAAAAACTTTCTCGCTTGTCTGCTGAGGAACAAGCCAAATTTCGCT TGGATAACACCCTTGGAGGCACGTCCGAAGTCATCCATCGAAAAGCACTCCAGAGGATATATGCTGACAA AGCAGCTGATATCATCGATGGCCTGAGGAAGAACCCCTCCATTGCTGTTCCGATTGTCCTTAAAAGGTTG AAGATGAAAGAAGAAGAGTGGCGAGAAGCTCAGAGAGGCTTCAACAAGGTCTGGCGAGAGCAAAATGAGA AGTACTACTTGAAGTCTCTGGATCACCAAGGCATCAACTTCAAGCAGAACGACACTAAGGTCTTGAGGTC TAAGAGCTTACTCAATGAGATCGAGAGCATCTATGACGAGAGGCAAGAGCAGGCTACAGAAGAGAACGCT GGTGTACCTGTTGGCCCGCACCTCTCTCTTGCCTATGAAGACAAACAGATACTAGAAGATGCTGCTGCTC TGATTATCCACCATGTGAAGAGGCAAACAGGCATTCAGAAAGAGGACAAATACAAAATCAAGCAAATCAT GCACCATTTCATTCCTGACCTGCTGTTTGCTCAGAGAGGCGATCTCTCAGATGTGGAAGAAGAGGAGGAG GAAGAAATGGATGTGGATGAAGCAACAGGAGCACCTAAGAAGCACAATGGTGTTGGGGGCAGCCCCCCTA AGTCCAAGTTGCTATTTAGTAACACAGCAGCTCAAAAGTTAAGAGGGATGGATGAAGTATATAACCTTTT CTATGTCAATAACAATTGGTATATCTTTATGCGACGGCATCAAATTCTCTGCTTGAGGCTGCTACGGATT TGTTTCCAAGCTGAACGGCAAATTGAAGAAGAAAACCGAGAGAGAGAATGGGAACGGGAGGTGCTAGGCA TAAAGCGAGACAAGAGTGATAGTCCTGCCATACAACTACGTCTCAAGGAACCTATGGATGTTGATGTAGA AGATTATTACCCAGCTTTCCTGGACATGGTGCGGAGCCTGCTTGATGGCAACATAGACTCATCACAGTAT GAAGATTCACTGAGAGAGATGTTCACCATTCATGCCTACATTGCCTTTACTATGGACAAATTAATCCAGA GCATCGTCAGACAGCTACAGCACATCGTCAGCGACGAGGTCTGTGTGCAGGTTACTGATCTTTACTTGGC AGAAAACAATAACGGAGCCACGGGAGGCCAGCTCAACAGTCAGACTTCAAGGAGCCTTCTGGAGTCAGCA TACCAGCGGAAGGCAGAGCAGCTTATGTCAGATGAGAACTGCTTCAAGCTAATGTTCATTCAAAGTCAAG GTCAAGTTCAGCTGACTGTTGAGCTCCTGGACACAGAAGAGGAGAACTCAGATGACCCCGTGGAAGCAGA GGTGTGGACACGTTGGTCAGACTACGTGGAGCGATATATGAGTTCTGATACTACTTCTCCTGAACTTCGA GAACATCTGGCACAGAAACCAGTATTTCTCCCAAGGAATTTGCGGCGTATCCGGAAGTGTCAACGTGGTC GAGAGCAACAGGAAAAAGAAGGGAAAGAAGGAAACAGCAAGAAGACCATGGAAAATGTAGAGGGCCTGGT ACCCTGCACCACCACCTGTGATACAGAGACTCTGCACTTTGTGAGCATTAACAAATATCGTGTCAAATAC GGCACAGTATTCAAAGCCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211790 protein sequence
Red=Cloning site Green=Tags(s) MKRRLDDQESPVYAAQQRRIPGSTEAFSHQHRVLAPAPPVYEAVSETMQSATGIQYSVAPNYQVSAVPQS SGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVEDALSYLDQVKLQFGSQ PQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGFNTFLPPGYKIEVQTNDMVNVTTPGQVHQ IPTHGIQPQPQPPPQHPSQPSSQSAPTPAQPAPQPTAAKVSKPSQLQAHTPASQQTPPLPPYASPRSPPV QPHTPVTISLGTAPSLQNNQPVEFNHAINYVNKIKNRFQGQPDIYKAFLEILHTYQKEQRNAKEAGGNYT PALTEQEVYAQVARLFKNQEDLLSEFGQFLPDANSSVLLSKTTAEKVDSVRNDHGGTVKKPQLNNKPQRP SQNGCQIRRHSGTGATPPVKKKPKLMSLKESSMADASKHGVGTESLFFDKVRKALRSAEAYENFLRCLVI FNQEVISRAELVQLVSPFLGKFPELFNWFKNFLGYKESVHLESFPKERATEGIAMEIDYASCKRLGSSYR ALPKSYQQPKCTGRTPLCKEVLNDTWVSFPSWSEDSTFVSSKKTQYEEHIYRCEDERFELDVVLETNLAT IRVLEAIQKKLSRLSAEEQAKFRLDNTLGGTSEVIHRKALQRIYADKAADIIDGLRKNPSIAVPIVLKRL KMKEEEWREAQRGFNKVWREQNEKYYLKSLDHQGINFKQNDTKVLRSKSLLNEIESIYDERQEQATEENA GVPVGPHLSLAYEDKQILEDAAALIIHHVKRQTGIQKEDKYKIKQIMHHFIPDLLFAQRGDLSDVEEEEE EEMDVDEATGAPKKHNGVGGSPPKSKLLFSNTAAQKLRGMDEVYNLFYVNNNWYIFMRRHQILCLRLLRI CFQAERQIEEENREREWEREVLGIKRDKSDSPAIQLRLKEPMDVDVEDYYPAFLDMVRSLLDGNIDSSQY EDSLREMFTIHAYIAFTMDKLIQSIVRQLQHIVSDEVCVQVTDLYLAENNNGATGGQLNSQTSRSLLESA YQRKAEQLMSDENCFKLMFIQSQGQVQLTVELLDTEEENSDDPVEAEVWTRWSDYVERYMSSDTTSPELR EHLAQKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVEGLVPCTTTCDTETLHFVSINKYRVKY GTVFKAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052716 |
| ORF Size | 3591 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052716, AAH52716 |
| RefSeq Size | 4820 bp |
| RefSeq ORF | 3593 bp |
| Locus ID | 20466 |
| MW | 135.8 kDa |
| Gene Summary | Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (PubMed:22476904). Required for cortical neuron differentiation and callosal axon elongation (PubMed:27399968).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223067 | Sin3a (untagged) - Mouse transcriptional regulator, SIN3A (yeast) (cDNA clone MGC:64685 IMAGE:6837144), (10ug) |
CNY 12,920.00 |
|
| MG211790 | Sin3a (tGFP-tagged) - Mouse transcriptional regulator, SIN3A (yeast) (cDNA clone MGC:64685 IMAGE:6837144) |
CNY 7,510.00 |
|
| MR211790L3 | Lenti ORF clone of Sin3a (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3A (yeast) (cDNA clone MGC:64685 IMAGE:6837144) |
CNY 8,740.00 |
|
| MR211790L4 | Lenti ORF clone of Sin3a (mGFP-tagged) - Mouse transcriptional regulator, SIN3A (yeast) (cDNA clone MGC:64685 IMAGE:6837144) |
CNY 8,740.00 |
