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Itga2 (NM_008396) Mouse Tagged ORF Clone
CAT＃: MR211760
- TrueORF®
Itga2 (Myc-DDK-tagged) - Mouse integrin alpha 2 (Itga2)
ORF Plasmid: tGFP
"NM_008396" in other vectors (4)
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CNY 8,200.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD49B; DX5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211760 representing NM_008396
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCGGGACAGGCAGGGGGCGCGCTCCTGCTGCGGCTGCTAATGCTAGTTCAAGGTATTTTGAATT GTCTGGCGTATAATGTTGGCCTCCCAGGAGCGAAAATATTTTCCGGGCCTTCAAGTGAACAGTTTGGGTA TTCAGTACAGCAGCTTACGAACCCACAAGGCAACTGGCTACTGGTTGGTTCACCGTGGAGTGGCTTCCCT GAGAACCGAATGGGAGATGTGTACAAGTGCCCTGTGGACCTACCCACTGCCACCTGTGAAAAGCTGAATC TGCAAAATTCAGCAAGCATCTCAAATGTTACCGAGATAAAGACCAACATGAGCCTGGGATTGACCCTCAC CAGGAATCCGGGGACCGGAGGCTTTCTAACCTGTGGTCCTCTCTGGGCACATCAGTGTGGAAATCAGTAT TATGCAACTGGGATCTGTTCTGATGTTAGTCCAGACTTTCAGTTCTTGACCAGCTTTTCACCTGCAGTTC AGGCTTGCCCTTCCCTCGTGGATGTTGTAGTTGTATGTGATGAATCAAACAGTATTTATCCTTGGGAAGC AGTAAAGAACTTTTTGGTAAAGTTTGTGACAGGCCTGGATATAGGACCTAAAAAGACACAGGTGGCGTTA ATTCAATATGCCAATGAGCCGAGAATTATATTTAACTTGAACGATTTCGAAACCAAAGAGGATATGGTCC AGGCCACATCTGAGACGCGCCAACATGGTGGGGACCTCACAAACACCTTCAGAGCTATCGAATTCGCAAG AGACTACGCTTATTCACAGACTTCTGGCGGGCGCCCGGGTGCTACAAAAGTCATGGTAGTTGTGACCGAT GGCGAGTCCCATGATGGGTCGAAGCTGAAAACTGTGATCCAGCAATGCAATGATGACGAGATACTGAGGT TCGGCATAGCAGTTCTTGGGTATTTAAACAGAAATGCTCTTGATACTAAAAATTTAATCAAAGAAATAAA AGCAATTGCTAGTACTCCAACCGAGAGATACTTTTTCAATGTGGCCGACGAAGCGGCTCTTCTGGAGAAG GCTGGAACTCTAGGGGAGCAAATATTCAGCATTGAAGGCACTGTTCAAGGAGGAGACAACTTCCAGATGG AAATGGCACAAGTTGGATTCAGTGCAGATTACGCTCCTCAAAATGATATTCTGATGTTAGGTGCAGTGGG GGCTTTTGACTGGAGTGGAACTCTTGTCCAGGAGACATCTCACAAGCCCGTGATCTTTCCTAAACAAGCC TTTGACCAGGTTCTGCAGGATAGAAACCACAGTTCGTTTTTAGGTTACTCTGTGGCTGCAATTTCTACTG AAGACGGTGTCCATTTTGTTGCTGGTGCTCCTCGAGCAAATTATACTGGCCAGATAGTGTTGTACAGCGT TAACAAGCAAGGCAATGTGACCGTGATTCAGTCTCACAGAGGGGACCAGATTGGCTCCTATTTTGGCAGT GTGCTGTGCTCAGTTGATGTAGATAAAGACACCATTACAGACGTGCTCCTGGTAGGTGCTCCTACATACA TGAACGACCTCAAGAAAGAAGAGGGAAAAGTCTACCTATTTACCATCACAAAGGGCATCTTGAATCAGCA CCAGTTTCTTGAAGGGCCAGAAGGCACTGGAAATGCTCGGTTTGGTTCAGCAATTGCAGCCCTGTCAGAC ATCAACATGGATGGCTTTAATGATGTGATTGTCGGTTCACCTGTAGAGAATGAGAACTCTGGAGCTGTGT ACATTTACAATGGTCACCAGGGTACCATTCGCACCAAGTACTCCCAGAAAATCCTTGGGTCCAATGGAGC CTTTAGGAGACATCTCCAGTTCTTTGGAAGGTCCCTGGATGGCTATGGTGATTTAAATGGGGATTCCATC ACTGATGTATCCATTGGTGCTTTGGGGCAAGTGATTCAACTCTGGTCACAAAGCATTGCTGACGTGGCCA TAGAAGCTTTGTTCACTCCAGACAAAATCACTTTGCTCAACAAGGATGCTAAGATAACCCTAAAGCTCTG CTTCAGAGCAGAGTTTAGACCTGCTGGGCAAAACAATCAAGTAGCCATCCTGTTTAACATGACGCTTGAT GCGGACGGACATTCATCCAGGGTAACCTCCAGGGGGGTGTTCAGAGAAAACAGTGAGCGATTCTTGCAGA AGAATATGGTGGTAAACGAAGTGCAGAAGTGTTCTGAGCACCACATTAGCATACAGAAGCCCTCTGATGT TGTCAACCCTTTGGATCTGCGTGTGGACATCAGTTTGGAAAACCCAGGCACTAGCCCTGCCCTCGAAGCC TATTCTGAAACTGTCAAAGTCTTCAGTATCCCTTTCTATAAGGAGTGTGGCAGCGATGGAATTTGCATCT CTGACCTGATCCTGGATGTCCAACAGCTGCCAGCCATTCAAACTCAATCCTTTATTGTCAGTAACCAAAA CAAGAGGTTAACATTTTCAGTCATCCTGAAAAACAGAGGGGAAAGTGCATACAACACTGTGGTCCTGGCC GAGTTTTCTGAGAACTTGTTTTTTGCTTCTTTCTCCATGCCGGTCGATGGAACAGAAGTAACATGTGAGG TTGGTTCATCGCAGAAATCTGTTACCTGTGATGTGGGATACCCTGCCTTAAAGAGTGAACAGCAGGTGAC TTTTACCATTAACTTCGACTTTAATCTTCAAAACCTCCAGAACCAGGCGGCCATCAATTTCCAAGCCTTC AGTGAGAGCCAAGAAACAAACAAAGCAGACAATTCGGTCAGTCTCACGATTCCTCTCCTGTATGATGCCG AACTTCACTTAACCCGATCCACCAACATCAACTTCTATGAGATCTCTTCGGATGAGAACGCTCCTTCTGT CATCAAGAGTGTCGAAGATATTGGTCCTAAATTCATCTTCTCCCTCAAGGTAACAGCCGGAAGTGCCCCA GTGAGCATGGCATTGGTGACTATCCACATTCCTCAGTACACCAAGGAAAAGAACCCACTCCTGTATCTGA CTGGGATACAAACAGACCAGGCTGGCGACATCAGCTGCACAGCAGAAATAAATCCACTCAAGCTACCACA CACCGCCCCTTCTGTATCTTTCAAGAATGAAAACTTCCGGCATACGAAAGAATTGGACTGCAGAACCACT TCCTGCAGTAACATCACCTGCTGGCTGAAAGACCTTCACATGAAAGCAGAGTACTTCATCAATGTGACAA CCAGAGTTTGGAACAGGACCTTTGCTGCTTCGACTTTCCAGACTGTGCAGCTGACAGCAGCGGCAGAGAT CGATACACATAACCCTCAGCTCTTCGTGATTGAGGAAAATGCTGTCACGATTCCCCTCATGATAATGAAA CCCACGGAGAAAGCAGAAGTACCGACAGGGGTTATCATAGGCAGCATAATTGCGGGAATCCTTTTGCTGT TGGCTATGACTGCAGGTTTGTGGAAGCTCGGCTTCTTCAAAAGACAATATAAGAAAATGGGCCAAAATCC AGATGAGATGGATGAGACCACAGAACTCAACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211760 representing NM_008396
Red=Cloning site Green=Tags(s) MGPGQAGGALLLRLLMLVQGILNCLAYNVGLPGAKIFSGPSSEQFGYSVQQLTNPQGNWLLVGSPWSGFP ENRMGDVYKCPVDLPTATCEKLNLQNSASISNVTEIKTNMSLGLTLTRNPGTGGFLTCGPLWAHQCGNQY YATGICSDVSPDFQFLTSFSPAVQACPSLVDVVVVCDESNSIYPWEAVKNFLVKFVTGLDIGPKKTQVAL IQYANEPRIIFNLNDFETKEDMVQATSETRQHGGDLTNTFRAIEFARDYAYSQTSGGRPGATKVMVVVTD GESHDGSKLKTVIQQCNDDEILRFGIAVLGYLNRNALDTKNLIKEIKAIASTPTERYFFNVADEAALLEK AGTLGEQIFSIEGTVQGGDNFQMEMAQVGFSADYAPQNDILMLGAVGAFDWSGTLVQETSHKPVIFPKQA FDQVLQDRNHSSFLGYSVAAISTEDGVHFVAGAPRANYTGQIVLYSVNKQGNVTVIQSHRGDQIGSYFGS VLCSVDVDKDTITDVLLVGAPTYMNDLKKEEGKVYLFTITKGILNQHQFLEGPEGTGNARFGSAIAALSD INMDGFNDVIVGSPVENENSGAVYIYNGHQGTIRTKYSQKILGSNGAFRRHLQFFGRSLDGYGDLNGDSI TDVSIGALGQVIQLWSQSIADVAIEALFTPDKITLLNKDAKITLKLCFRAEFRPAGQNNQVAILFNMTLD ADGHSSRVTSRGVFRENSERFLQKNMVVNEVQKCSEHHISIQKPSDVVNPLDLRVDISLENPGTSPALEA YSETVKVFSIPFYKECGSDGICISDLILDVQQLPAIQTQSFIVSNQNKRLTFSVILKNRGESAYNTVVLA EFSENLFFASFSMPVDGTEVTCEVGSSQKSVTCDVGYPALKSEQQVTFTINFDFNLQNLQNQAAINFQAF SESQETNKADNSVSLTIPLLYDAELHLTRSTNINFYEISSDENAPSVIKSVEDIGPKFIFSLKVTAGSAP VSMALVTIHIPQYTKEKNPLLYLTGIQTDQAGDISCTAEINPLKLPHTAPSVSFKNENFRHTKELDCRTT SCSNITCWLKDLHMKAEYFINVTTRVWNRTFAASTFQTVQLTAAAEIDTHNPQLFVIEENAVTIPLMIMK PTEKAEVPTGVIIGSIIAGILLLLAMTAGLWKLGFFKRQYKKMGQNPDEMDETTELNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008396 |
| ORF Size | 3534 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008396.3 |
| RefSeq Size | 4235 bp |
| RefSeq ORF | 3537 bp |
| Locus ID | 16398 |
| UniProt ID | Q62469 |
| MW | 129.4 kDa |
| Gene Summary | Integrin alpha-2/beta-1 is a collagen receptor, being responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides and E-cadherin. Mice homozygous for a null mutation in the alpha-2 die very early in embryogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223813 | Itga2 (untagged) - Mouse integrin alpha 2 (Itga2), (10ug) |
CNY 15,110.00 |
|
| MG211760 | Itga2 (tGFP-tagged) - Mouse integrin alpha 2 (Itga2) |
CNY 11,400.00 |
|
| MR211760L3 | Lenti ORF clone of Itga2 (Myc-DDK-tagged) - Mouse integrin alpha 2 (Itga2) |
CNY 12,260.00 |
|
| MR211760L4 | Lenti ORF clone of Itga2 (mGFP-tagged) - Mouse integrin alpha 2 (Itga2) |
CNY 12,260.00 |
