Mybpc2 (NM_146189) Mouse Tagged ORF Clone
CAT#: MR211692
- TrueORF®
Mybpc2 (Myc-DDK-tagged) - Mouse myosin binding protein C, fast-type (Mybpc2), transcript variant 2
"NM_146189" in other vectors (2)
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CNY 7,920.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC28149 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211692 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAGGCTAAACCAGCGGCCAAAAAGGCCTCCAAAGGCAAAGATGCCCCAAAGGAAGCCCCTGCAA AGCAGACTCCTGAAGAGCCCCCCAAAGAGGCCCCACCTGAGGACCAATCCCCAACTGCGGAGGAGCCCAC AGGCATCTTCCTGAAAAAGCCGGACTCTGTGTCAGTGGAGACTGGGAAAGACGCGGTGATTCTGGCCAAG GTGAACGGGAAGGAGCTCCCAGGCAAGCCGACCATCAAGTGGTTCAAGGGGAAGTGGCAGGAGCTGGGCA GCAAGAGCGGAGCCCGGTTCATCTTCAAGGAATCTCATGACTCTACCAGCAATGTGTACACCGTGGAGCT CCACATTGGGAAGGTGGTCCTAGGGGACCGCGGGGATTACCGTCTAGAGATCAAAGCCAAGGATGTCTGC GACAGCTGCTCGTTCAACGTGGATGTGGAGGCACCCCGTCAGGACTCATCCGGTCAGAGCCTCGAGAGCT TCAAGCGTTCGGGTGACGGGAAGTCAGAAGATGCAGGCGAGCTGGATTTCAGTGGCTTGCTGAAGAAGAG GGAAGTAGTTGAGGAGGAGAAGAAGAAGAAGAAAGATGATGATGATCTGGGTATACCTCCGGAGATTTGG GAGCTCCTGAAAGGGGCCAAGAAGAGCGAGTACGAGAAGATCGCCTTCCAGTACGGCATCACTGACCTCC GTGGCATGCTGAAGCGGCTCAAGAAGGCCAAGGTGGAGGTCAAGAAGAGTGCAGCCTTCACTAAGAAGTT GGACCCAGCCTACCAAGTAGACAGGGGCAACAAGATCAAGTTGGTGGTGGAGATCAGTGACCCAGACCTT CCTCTCAAGTGGTTCAAGAATGGCCAGGAGATCAAACCAAGCAGCAAGTATGTGTTTGAGAACGTTGGGA AGAAACGAATTCTTACCATCAACAAGTGCACGCTGGCGGATGATGCTGCATATGAGGTGGCAGTCCAAGA TGAGAAGTGTTTCACTGAACTCTTTGTCAAAGAGCCCCCAGTCTTGATTGTCACCCCACTGGAGGACCAG CAGGTATTCGTAGGTGACCGAGTGGAAATGTCGGTAGAGGTGTCAGAAGAGGGTGCACAGGTTATGTGGA TGAAAGATGGTGTGGAGATGACTCGGGAGGATTCCTACAAGGCGCGCTACCGCTTCAAGAAGGACGGGAA ACGGCACATCCTCATCTACTCTGATGTGGCCCAGGAGGATGGGGGCCGCTACCAGGTCATAACCAATGGT GGCCAGTGTGAGGCTGAACTCATTGTGGAAGAGAAGCAACTGGAGGTCCTGCAGGACATTGCTGACCTGA CAGTGAAGGCTGCAGAGCAGGCTGTGTTCAAGTGTGAAGTGTCTGATGAGAAGGTGACAGGCAAATGGTA CAAGAATGGCGTGGAGGTACGACCCAGCAAGAGGATCACCATCTCCCATGTGGGCAGATTCCACAAGCTG GTGATTGATGACGTCCGCCCTGAGGATGAGGGAGACTACACATTCGTGCCCGATGGCTATGCCCTATCCC TCTCAGCCAAGCTCAACTTTTTGGAAATCAAAGTGGAGTACGTACCTAAGCAAGAGCCCCCGAAGATCCA CCTGGACTGCTCAGGGAAGACCTCAGATAACTCAATTGTTGTGGTGGCTGGAAACAAGCTGCGGCTGGAT GTGGCCATCACAGGGGAACCACCTCCCACTGCTACCTGGCTAAGGGGAGATGAGGTGTTCACAGCCACAG AAGGCAGGACACACATCGAGCAGAGGCCAGACTGCAGCAGCTTTGTGATCGAGAGTGCGGAGCGGTCGGA TGAGGGCCGCTACACCATCAAAGTCACCAATCCTGCGGGCGAGGACGTGGCCTCCATTTTCCTGCGGGTT GTGGATGTTCCTGATCCTCCAGAGGCTGTCCGTGTCACCTCAGTTGGAGAAGACTGGGCCATTCTGGTCT GGGAGCCACCCAAGTATGATGGAGGACAGCCAGTGACTGGGTACTTGATGGAGCGGAAGAAGAAAGGCTC CCAGCGCTGGATGAAGATCAACTTTGAGGTCTTCACTGACACCACCTACGAGTCCACAAAGATGATCGAG GGCGTCCTTTATGAGATGCGGGTCTTCGCTGTCAACGCCATTGGGGTCTCACAGCCCAGCATGAACACCA AGCCCTTCATGCCCATTGCTCCCACGAGTGCACCACAACACCTCACCGTGGAGGATGTGACGGACACCAC CACCACACTTAAGTGGAGACCTCCAGATCGGATTGGTGCTGGTGGCATTGATGGCTACCTGGTGGAGTAC TGCCTGGAGGGCTCTGAGGAATGGGTACCGGCTAACAAGGAGCCAGTGGAACGCTGTGGCTTCACTGTCA AGGATCTCCCAACTGGAGCCAGGATCCTCTTCCGGGTTGTTGGGGTCAACATCGCAGGGCGCAGTGAACC AGCCACCCTCCTTCAGCCAGTCACTATCAGGGAGATTGTTGAGCAACCTAAGATCCGCCTTCCCCGCCAT CTTCGTCAGACTTATATCCGAAAAGTTGGGGAGGCCCTCAACCTCGTCATCCCCTTCCAGGGCAAGCCCC GGCCTCAGGTGGTGTGGACCAAGGGTGGGGCTCCCTTGGATACCTCCCGTGTGAATGTGCGGACTAGTGA CTTCGACACAGTGTTCTTCGTGCGACAGGCGGCCCGCTCTGACTCCGGAGAGTATGAGCTGAGTGTGCAG ATTGAAAACATGAAGGACACTGCTACCATACGCATCCGGGTCGTGGAAAAGGCAGGGCCAGCAGAGAATG TGATGGTGAAGGAGGTGTGGGGCACAAATGCTCTGGTGGAGTGGCAGCCGCCCAAGGACGACGGGAACAG TGAGATCACAGGCTACTTTGTCCAGAAGGCTGACAAAAAGACCATGGAGTGGTTCAATGTCTACGAACAC AATCGCCACACCAGCTGTACGGTGTCTGACCTCATTGTGGGTAATGAGTACTACTTCCGTATCTTCAGCG AGAACATCTGCGGTCTCAGTGACTCGCCAGGTGTCTCCAAGAACACAGCTCGAATTCTCAAGACAGGAAT CACCTTAAAACCACTGGAGTACAAGGAGCATGATTTCCGGACGGCTCCCAAGTTCCTGACCCCGCTGATG GACCGGGTCGTGGTGGCGGGATATACCGCAGCTCTCAACTGTGCCGTCAGAGGCCACCCTAAGCCAAAGG TGGTGTGGATGAAGAACAAGATGGAAATCCATGAAGATCCCAAATTCCTCATAACCAACTACCAGGGCAT CCTGACACTGAACATCCGCCGACCGTCACCCTTTGATGCAGGGACCTATTCCTGCCGCGCCTTCAATGAG CTGGGGGAGGCCCTGGCTGAATGCAAACTGGATGTCCGAGTGCCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211692 protein sequence
Red=Cloning site Green=Tags(s) MPEAKPAAKKASKGKDAPKEAPAKQTPEEPPKEAPPEDQSPTAEEPTGIFLKKPDSVSVETGKDAVILAK VNGKELPGKPTIKWFKGKWQELGSKSGARFIFKESHDSTSNVYTVELHIGKVVLGDRGDYRLEIKAKDVC DSCSFNVDVEAPRQDSSGQSLESFKRSGDGKSEDAGELDFSGLLKKREVVEEEKKKKKDDDDLGIPPEIW ELLKGAKKSEYEKIAFQYGITDLRGMLKRLKKAKVEVKKSAAFTKKLDPAYQVDRGNKIKLVVEISDPDL PLKWFKNGQEIKPSSKYVFENVGKKRILTINKCTLADDAAYEVAVQDEKCFTELFVKEPPVLIVTPLEDQ QVFVGDRVEMSVEVSEEGAQVMWMKDGVEMTREDSYKARYRFKKDGKRHILIYSDVAQEDGGRYQVITNG GQCEAELIVEEKQLEVLQDIADLTVKAAEQAVFKCEVSDEKVTGKWYKNGVEVRPSKRITISHVGRFHKL VIDDVRPEDEGDYTFVPDGYALSLSAKLNFLEIKVEYVPKQEPPKIHLDCSGKTSDNSIVVVAGNKLRLD VAITGEPPPTATWLRGDEVFTATEGRTHIEQRPDCSSFVIESAERSDEGRYTIKVTNPAGEDVASIFLRV VDVPDPPEAVRVTSVGEDWAILVWEPPKYDGGQPVTGYLMERKKKGSQRWMKINFEVFTDTTYESTKMIE GVLYEMRVFAVNAIGVSQPSMNTKPFMPIAPTSAPQHLTVEDVTDTTTTLKWRPPDRIGAGGIDGYLVEY CLEGSEEWVPANKEPVERCGFTVKDLPTGARILFRVVGVNIAGRSEPATLLQPVTIREIVEQPKIRLPRH LRQTYIRKVGEALNLVIPFQGKPRPQVVWTKGGAPLDTSRVNVRTSDFDTVFFVRQAARSDSGEYELSVQ IENMKDTATIRIRVVEKAGPAENVMVKEVWGTNALVEWQPPKDDGNSEITGYFVQKADKKTMEWFNVYEH NRHTSCTVSDLIVGNEYYFRIFSENICGLSDSPGVSKNTARILKTGITLKPLEYKEHDFRTAPKFLTPLM DRVVVAGYTAALNCAVRGHPKPKVVWMKNKMEIHEDPKFLITNYQGILTLNIRRPSPFDAGTYSCRAFNE LGEALAECKLDVRVPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_146189 |
ORF Size | 3411 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146189.1 |
RefSeq Size | 3624 bp |
RefSeq ORF | 3411 bp |
Locus ID | 233199 |
UniProt ID | Q5XKE0 |
MW | 127.3 kDa |
Gene Summary | Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR211692L3 | Lenti ORF clone of Mybpc2 (Myc-DDK-tagged) - Mouse myosin binding protein C, fast-type (Mybpc2), transcript variant 2 |
CNY 11,780.00 |
|
MR211692L4 | Lenti ORF clone of Mybpc2 (mGFP-tagged) - Mouse myosin binding protein C, fast-type (Mybpc2), transcript variant 2 |
CNY 11,780.00 |