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Ascc3 (BC059917) Mouse Tagged ORF Clone
CAT＃: MR211675
- TrueORF®
Ascc3 (Myc-DDK-tagged) - Mouse activating signal cointegrator 1 complex subunit 3 (cDNA clone MGC:69928 IMAGE:6826790)
ORF Plasmid: tGFP
"BC059917" in other vectors (4)
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CNY 11,210.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RNAH, ASC1p200, D630041L21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211675 representing BC059917
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTTTCTCCCTCATATCAGATTCTGCATATGTCGCCCAGAATGCAGCTAGAATTGTCCGTGCTC TCTTTGAAATTGCACTGAGGAAACGTTGGCCTACCATGACCTACAGGCTTTTGAATCTTAGTAAAGTCAT TGACAAGAGACTCTGGGGTTGGGCTAGCCCTTTGAGACAATTTTCTGTGTTACCACCACACATTCTAACA AGATTAGAAGAAAAAAACCTTACTGTGGATAAACTCAAAGACATGAGAAAAGATGAAATAGGTCACATTT TGCATCATGTGAATATTGGACTGAAGGTCAAACAGTGTGTTCATCAAATTCCTTCTGTTACAATGGAAGC TTCCATTCAGCCCATCACACGGACTGTCCTCCGAGTATCACTGAACATCCACCCTGATTTCTCTTGGAAT GACCAGGTTCATGGAACAGTGGGGGAACCATGGTGGATTTGGGTAGAAGATCCTACAAATGACCATATTT ATCACTCGGAGTACTTTCTAGCTCTTAAAAAACAGGTTATTAATAAGGAAGCTCAACTTCTGGTATTTAC AATTCCTATTTTTGAGCCTTTGCCCTCTCAGTACTACATTCGAGCAGTGTCTGATCGATGGCTAGGAGCT GAAGCTGTTTGCATTATCAACTTTCAACATCTGATTCTACCAGAGAGACATCCTCCCCATACAGAATTAT TGGACCTTCAACCTTTACCAATTACAGCTTTGGGGTGCAAAGCATATGAAGCTCTATATAACTTCAGCCA CTTTAACCCTGTACAGACGCAGATATTCCACACACTGTATCACACAGACTGTAATGTTCTGCTTGGCGCC CCCACCGGATCAGGAAAGACAGTTGCAGCTGAACTAGCCATTTTCAGAGTTTTCAACAAATATCCTACTT CAAAGGCAGTATATATTGCACCCCTCAAAGCTCTAGTACGTGAAAGAATGGATGATTGGAAAATTAGGAT AGAAGAAAAACTTGGTAAAAAAGTTATTGAACTAACTGGGGATGTGACTCCAGACATGAAATCCATTGCT AAGGCTGACCTTATTGTCACCACACCAGAAAAGTGGGATGGAGTTAGTCGAAGCTGGCAGAATAGAAGCT ATGTTCAGCAGGTCAACATTCTCATCATAGATGAGATCCATCTGCTAGGGGAGGAGAGAGGCCCAGTTTT AGAAGTCATTGTATCTCGAACAAACTTTATCTCATCACACACAGAAAAACCAGTTAGAATAGTTGGACTG TCCACTGCATTAGCTAATGCTAGAGATCTTGCTGACTGGCTCAACATTAAGCAGATGGGTTTGTTTAACT TCCGACCATCAGTTCGCCCAGTTCCACTGGAAGTTCACATTCAGGGTTTCCCAGGACAACATTACTGCCC ACGCATGGCTAGTATGAACAAGCCTGCATTTCAGGCAATTAGAAGTCACTCTCCAGCCAAGCCTGTTTTG ATATTTGTCTCATCAAGACGTCAAACACGACTTACAGCCTTAGAGTTGATAGCATTTCTGGCCACTGAAG AAGACCCAAAGCAGTGGTTGAATATGGATGAGCAAGAGATGGACAACATCATTGGAACAGTACGAGATTC CAACCTCAAGCTGACTCTAGCTTTTGGAATAGGAATGCATCATGCTGGCCTGCATGAAAGAGACCGAAAA ACTGTAGAAGAGCTGTTTGTGAACTGTAAAGTTCAGGTTCTCATTGCTACAAGCACATTAGCATGGGGTG TAAACTTTCCAGCACACTTAGTAATTATTAAGGGAACTGAATATTATGATGGAAAGACAAGACGTTATGT GGATTTTCCCATCACAGATGTGCTGCAGATGATGGGACGAGCTGGGAGACCTCAGTTTGATGACCAGGGC AAAGCTGTAATTCTAGTTCATGATATAAAAAAAGATTTTTATAAAAAGTTTCTTTATGAACCTTTCCCAG TAGAATCAAGCTTATTAGGAGTGTTGTCTGACCACTTAAATGCAGAGATTGCTGGAGGTACAATAACATC AAAGCAAGATGCAATGGATTATATCACCTGGACTTACTTTTTCCGGCGTCTTATCATGAATCCCAGCTAC TACAGCTTGGGTGACGTCAGCCAGGACTCCATAAACAAGTTCCTGTCACATCTGATTGGGCAGTCTCTGG TTGAATTGGAGCTTTCCCACTGCATTGAAGTTGGAGAGGATAATCGCACCATTGAACCACTGACATGTGG TCTAATTGCCTCCTATTACTATTTGAAGCACAAAACTGTCAAAATGTTCAAGGACCGTTTGAAACCTGAG TGCAGCACTGAGGAACTGCTGTCAATTCTGAGTGATGCAGAAGAATACACAGACTTACCAGTAAGACACA ATGAGGACCATACGAATAATGAATTGGCCAAATGTCTTCCCATTGAATTAAATCCTCATTCATTTGACAG CCCTCACACCAAAGCCCATCTCTTGCTTCAAGCGCATCTCAGCCGAGCCATGCTGCCCTGCCCAGATTAT GATACTGATACCAAAACAGTCTTAGACCAAGCTCTCAGAGTATGCCAGGCAATGCTGGATGTGGCTGCAA GCCAGGGATGGCTAGTGACCGTCCTGAATATCACCCACCTGATTCAGATGGTGATCCAAGGCCGATGGCT CAAGGACTCTTCTCTTCTCACAATACCAAACATAGAGCAGCACCACCTTCACCTTTTCAGGAAATGGAAG CCACCTGTAAAGAGCTCACATGCTAAGTGCCGAACATCCATTGAGTGCCTTCCTGAGCTGATCCATGCCT GTGAAGGGAAGGACCATGTGTTCAGCTCCATGGTAGAGAAAGAGTTACAGCCTGCAAAAACAAAGCAGGC ATGGAATTTCTTATCTCGCTTGCCAGTGATAAATGTTGGCATAAGTGTTAAAGGCTCATGGGATGACTTA GTTGAAGGACATAATGAACTCTCCATCTCAACTCTGACAGCAGACAAACGGGATGAAAACAAATGGATCA AGTTGCATGCTGATCAGGAGTATGTGCTTCAAGTCAGCCTACAGAGAGTCCATTTTGGGCTTCCAAAGGG AAAACATGAGAATCACGCAGTTACTCCTCGATTTCCAAAATTAAAAGATGAAGGCTGGTTTTTGATATTA GGAGAAGTGGATAAGAGAGAGCTGATGGCTGTAAAACGAGTAGGATTTGTTCGAACACATCATGATGCTT CCATCTCGTTCTTTACTCCTGAAACACCTGGAAGATATATCTTTACCCTGTATCTCATGAGTGACTGTTA CCTCGGCCTAGACCAGCAGTATGACATTTATCTCAATGTCATAAAAGCAAACATTTCTACAAAGGACTCT GATGTCTTCACTGACTTGTCAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211675 representing BC059917
Red=Cloning site Green=Tags(s) MDSFSLISDSAYVAQNAARIVRALFEIALRKRWPTMTYRLLNLSKVIDKRLWGWASPLRQFSVLPPHILT RLEEKNLTVDKLKDMRKDEIGHILHHVNIGLKVKQCVHQIPSVTMEASIQPITRTVLRVSLNIHPDFSWN DQVHGTVGEPWWIWVEDPTNDHIYHSEYFLALKKQVINKEAQLLVFTIPIFEPLPSQYYIRAVSDRWLGA EAVCIINFQHLILPERHPPHTELLDLQPLPITALGCKAYEALYNFSHFNPVQTQIFHTLYHTDCNVLLGA PTGSGKTVAAELAIFRVFNKYPTSKAVYIAPLKALVRERMDDWKIRIEEKLGKKVIELTGDVTPDMKSIA KADLIVTTPEKWDGVSRSWQNRSYVQQVNILIIDEIHLLGEERGPVLEVIVSRTNFISSHTEKPVRIVGL STALANARDLADWLNIKQMGLFNFRPSVRPVPLEVHIQGFPGQHYCPRMASMNKPAFQAIRSHSPAKPVL IFVSSRRQTRLTALELIAFLATEEDPKQWLNMDEQEMDNIIGTVRDSNLKLTLAFGIGMHHAGLHERDRK TVEELFVNCKVQVLIATSTLAWGVNFPAHLVIIKGTEYYDGKTRRYVDFPITDVLQMMGRAGRPQFDDQG KAVILVHDIKKDFYKKFLYEPFPVESSLLGVLSDHLNAEIAGGTITSKQDAMDYITWTYFFRRLIMNPSY YSLGDVSQDSINKFLSHLIGQSLVELELSHCIEVGEDNRTIEPLTCGLIASYYYLKHKTVKMFKDRLKPE CSTEELLSILSDAEEYTDLPVRHNEDHTNNELAKCLPIELNPHSFDSPHTKAHLLLQAHLSRAMLPCPDY DTDTKTVLDQALRVCQAMLDVAASQGWLVTVLNITHLIQMVIQGRWLKDSSLLTIPNIEQHHLHLFRKWK PPVKSSHAKCRTSIECLPELIHACEGKDHVFSSMVEKELQPAKTKQAWNFLSRLPVINVGISVKGSWDDL VEGHNELSISTLTADKRDENKWIKLHADQEYVLQVSLQRVHFGLPKGKHENHAVTPRFPKLKDEGWFLIL GEVDKRELMAVKRVGFVRTHHDASISFFTPETPGRYIFTLYLMSDCYLGLDQQYDIYLNVIKANISTKDS DVFTDLSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059917 |
| ORF Size | 3384 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059917.1 |
| RefSeq Size | 4162 bp |
| RefSeq ORF | 3386 bp |
| Locus ID | 77987 |
| MW | 152.6 kDa |
| Gene Summary | 3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223107 | Ascc3 (untagged) - Mouse activating signal cointegrator 1 complex subunit 3 (cDNA clone MGC:69928 IMAGE:6826790), (10ug) |
CNY 12,920.00 |
|
| MG211675 | Ascc3 (tGFP-tagged) - Mouse activating signal cointegrator 1 complex subunit 3 (cDNA clone MGC:69928 IMAGE:6826790) |
CNY 12,350.00 |
|
| MR211675L3 | Lenti ORF clone of Ascc3 (Myc-DDK-tagged) - Mouse activating signal cointegrator 1 complex subunit 3 (cDNA clone MGC:69928 IMAGE:6826790) |
CNY 13,110.00 |
|
| MR211675L4 | Lenti ORF clone of Ascc3 (mGFP-tagged) - Mouse activating signal cointegrator 1 complex subunit 3 (cDNA clone MGC:69928 IMAGE:6826790) |
CNY 13,110.00 |
