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Anln (NM_028390) Mouse Tagged ORF Clone
CAT＃: MR211659
- TrueORF®
Anln (Myc-DDK-tagged) - Mouse anillin, actin binding protein (Anln)
ORF Plasmid: tGFP
"NM_028390" in other vectors (3)
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CNY 7,808.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110037A17Rik; 2900037I21Rik; C78101; Scraps |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211659 representing NM_028390
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCGTTTACCGAGAAGTTGCTAGAACGAACTCGTGCCAGACGAGAGAATCTTCAGAGAAAAATGG CTGAGAGGCCTACGGCAGTAGCGAGATCTGCCCCGCATGCGAAGAGAGGCAGAGAGCCACTTTCAGAAGC AAGTAATCAGCAGCAGCCCCTACCAGGGGGCGAAGAAAAATCTTGTACAAAACCATCACCATCAAAAAAA CGTTGTTCTGACAAAATTGAAGTGGGAGCTCCGGACTTAGAAAATACAGAACCTATTGATGTTGCAAAGC CCTGTTCTCCGATGCCTGCACCCCGGCAGGCGAAGCCCCCAGCACCAGCTGCCATCAGCGAGTCTGTGGC TGCCCCAGCAGCCCTGCTCAGCGCGGACAGAGGGCTGAACTCAGGATCCGAAGCATCTGCAACCTCCTCA GTTAAAACTCGAATGCAAAGGCTTGCTGAGCAGCGGCGCCATTGGGATAGTGATCTCACAGATGATGTAT CAGAAAGTTCATACTTTGCACCAGTGCCAACTGAGGACAAGGCTGCCTCACCTTCTAAGCCACCCATTTC AAATGCCTCAGCTACTCCAGTTGGGAGAAGGGGCCGTCTGGCCAACCTTGCTGCAACGATTTGCTCCTGG GAAGATGATGTAAGCCACTCATCTGCAAAGCAAAATAGTGTGCAAGAACAGCCTGGTACCGCTTGTTTAT CCAAATCTTCCTCTGCAAGTGGAGCATCTGCTAGCATCAATAGCAGCAGTGTTCAGCAGGAAGCTACATG CTGTTCCCCAAGGGACGGCAATGCCTCTGTCAGGAAAGACCCATCTTCAAATGCTGCCCATGGACCTTTG CTTAGCGCCTCAGTGTCCAGCTCTGTGAAAGCGTCTTCCCCTGTGACAGCTGCTACCTTCATCACTGAAA ACCGTGAGGCACAAAATCCTGAGCTACTTCACAAAACTGCTAGTCCTCTGAAAACAGAGGCGCGGAAACC ATGTGAGAAGCCAACTTTGTCCCAGGGAGCTCAGCCCAAAGAGGAGGCTAACAGAGAAGTTTGTCTACAG TCACAATCCAAGGACAAACTTGCAACACCAGGAGGAAGAGGAATTAAGCCTTTCCTGGAACGCTTTGGAG AGCGTTGTCAAGAACACAGTAAAGAAAGTCCGTCTTATAGAGCATCTCATAAAACCCCAAATATCACTCC AAATACAAAAGCCATCCAGGAAAGATTATTCAAACAAAACACATGCTCGTCTACTACCCATTTAGCACAG CAGCTCAAACAGGAACGTGAAAAAGAACTGGCATGTCTTCGTGGTCGACTTGACAAGGGCAATTTATGGA GTGCAGAAAAGAATGAAAAGTCAAGAAGCAAGCATCTAGAAACCAAACAGGAAGTTCACTGTCAGAACAC TCCACTCAAGAAACATCAAACTGTCGCAAGCACCCCATTGACTTCTGTAACAGATAAGGTGGCTGAAAAT GAACCAGCAGTGAAGCTTTCTAGCACAGAGCCTGCAGGTTCCACTGAAAGCGAAATGACAAAGTCCAGCC CTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAAAGTAGCATCAGACCTGGAGGTTGAGCA GAACACTGAAGCAGTGCGTGAGGTTGAGATGAGTGTGGACGATGAGGACATCAATAGCTCCAGAGTCATT AACGACATCTTCAGTGACGTCCTAGAGGAAGGGGAGCTGGATGTGGAAAAGAGCCAAGAGGAGATGGACC AAGTGGGAGCAGAAAACAGTGAGGAGCAGGAAGATGCGCTCAATATCTCTTCAATGTCTTTACTTGCTCC GCTAGCTCAGACGGTCGGTGTGGTGAGCCTAGAGAATGTAATTTCTTCACCTCCGTCGGAATTGAGAGAC TCTAACCTAAGCGCTGCAAGTCCTAAGCCCGGGAAATTCCAGAGAACCCGCGTCCCTCGCGCCGAATCTG CCGATAGCCTCGGTTCTGAGGACCGGGACCTTCTCTATAGCATTGATGCATATAGGTCTCAAAGATTCAA AGAAACAGAACGCCCTTCCATAAAGCAAGTGATTGTTCGAAAGGAAGATGTTACTTCAAAGTTGGGTGAA AAGAAAAACGTATTTTCTGGTCAAGTTAATATCAAACAAAAAATGCAGGAGCTCAATAATGACATAAATT TGCAGCAGACAGTGATCTATCAGGCCAGTCAGGCTCTCAACTGCTGTGTGGATGAAGAACACGGGAAAGG ATCCTTGGAAGAAGCTGAAGCAGAAAGACTTCTTCTGATTGCAACTGAGAAAAGAGCACTTCTGATTGAT GAGCTGAATAAGCTGAAGAGTGAAGGACCTCAGAGGAGAAACAAGACCAGTGTCATATCCCAGAGTGAAT TTGCTCCATCTAAAGGGTCAGTCACTCTGTCAGAAATCTGCTTGCCTCTGAAGGCAGATTTTGTCTGCAG CACTGCGCAAAAAACAGATGCATCAAATTATTACTACTTAATTATGCTAAAAGCTGGGGCTGAGCAGATG GTCGCCACTCCATTAGCAAGTACTGCAAACTCTCTCAGTGGTGACGCTCTGACATTTCCTACTACATTTA CTCTGCATGATGTTTCCAATGACTTTGAAATAAACATTGAAGTTTACAGCCTGGTACAAAAGAAAGATTC CTTGGGCCCCGATAAGAAGAAGAAAGCCTCCAAGTCCAAGGCTATTACTCCAAAGAGACTCCTCACATCT ATAACTTCAAAAAGCAGCCTTCATTCTTCAGTTATGGCCAGTCCCGGAGGTCTCGGTGCTGTGCGTACCA GCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTCTGTTGGAGACACTAAGTTTGCTTTGGA CAAGGTACCTTTTTTGTCTCCGTTGGAAGGTCACATCTGTTTAAAAATAAGCTGTCAAGTGAATTCAGCT GTTGAGGAAAAGGGTTTCCTTACCATATTTGAAGATGTTAGTGGCTTTGGTGCCTGGCACCGAAGATGGT GTGTTCTCTCTGGCAACTGTATCTCTTACTGGACTTACCCAGATGATGAGAGGCGAAAGAATCCCATAGG AAGGATAAATCTGGCCAATTGTATCAGTCATCAGATAGAACCAGCCAACAGAGAATTTTGTGCAAGACGC AACACTCTGGAATTGATTACTGTCCGACCACAAAGAGAAGACGATCGAGAAACTCTTGTCAGCCAATGTA GAGACACACTCTGTGTCACCAAGAACTGGCTCTCTGCAGATACTAAAGAAGAGCGGGATCTCTGGATGCA GAAACTCAACCAGGTCATTGTTGATATTCGCCTCTGGCAGCCTGATGCATGCTACAAGCCTGTTGGGAAG CCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211659 representing NM_028390
Red=Cloning site Green=Tags(s) MDPFTEKLLERTRARRENLQRKMAERPTAVARSAPHAKRGREPLSEASNQQQPLPGGEEKSCTKPSPSKK RCSDKIEVGAPDLENTEPIDVAKPCSPMPAPRQAKPPAPAAISESVAAPAALLSADRGLNSGSEASATSS VKTRMQRLAEQRRHWDSDLTDDVSESSYFAPVPTEDKAASPSKPPISNASATPVGRRGRLANLAATICSW EDDVSHSSAKQNSVQEQPGTACLSKSSSASGASASINSSSVQQEATCCSPRDGNASVRKDPSSNAAHGPL LSASVSSSVKASSPVTAATFITENREAQNPELLHKTASPLKTEARKPCEKPTLSQGAQPKEEANREVCLQ SQSKDKLATPGGRGIKPFLERFGERCQEHSKESPSYRASHKTPNITPNTKAIQERLFKQNTCSSTTHLAQ QLKQEREKELACLRGRLDKGNLWSAEKNEKSRSKHLETKQEVHCQNTPLKKHQTVASTPLTSVTDKVAEN EPAVKLSSTEPAGSTESEMTKSSPLKITLFLEEEKSLKVASDLEVEQNTEAVREVEMSVDDEDINSSRVI NDIFSDVLEEGELDVEKSQEEMDQVGAENSEEQEDALNISSMSLLAPLAQTVGVVSLENVISSPPSELRD SNLSAASPKPGKFQRTRVPRAESADSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLGE KKNVFSGQVNIKQKMQELNNDINLQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATEKRALLID ELNKLKSEGPQRRNKTSVISQSEFAPSKGSVTLSEICLPLKADFVCSTAQKTDASNYYYLIMLKAGAEQM VATPLASTANSLSGDALTFPTTFTLHDVSNDFEINIEVYSLVQKKDSLGPDKKKKASKSKAITPKRLLTS ITSKSSLHSSVMASPGGLGAVRTSNFTLVGSHTLSLSSVGDTKFALDKVPFLSPLEGHICLKISCQVNSA VEEKGFLTIFEDVSGFGAWHRRWCVLSGNCISYWTYPDDERRKNPIGRINLANCISHQIEPANREFCARR NTLELITVRPQREDDRETLVSQCRDTLCVTKNWLSADTKEERDLWMQKLNQVIVDIRLWQPDACYKPVGK P TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028390 |
| ORF Size | 3363 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028390.3, NP_082666.2 |
| RefSeq Size | 5421 bp |
| RefSeq ORF | 3366 bp |
| Locus ID | 68743 |
| UniProt ID | Q8K298 |
| MW | 122.8 kDa |
| Gene Summary | Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211659 | Anln (tGFP-tagged) - Mouse anillin, actin binding protein (scraps homolog, Drosophila) (Anln) |
CNY 9,408.00 |
|
| MR211659L3 | Lenti ORF clone of Anln (Myc-DDK-tagged) - Mouse anillin, actin binding protein (Anln) |
CNY 10,260.00 |
|
| MR211659L4 | Lenti ORF clone of Anln (mGFP-tagged) - Mouse anillin, actin binding protein (Anln) |
CNY 10,260.00 |
