Col4a6 (BC057648) Mouse Tagged ORF Clone

CAT#: MR211656

  • TrueORF®

Col4a6 (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 6 (cDNA clone MGC:67937 IMAGE:3500535)

ORF Plasmid: DDK tGFP



  "BC057648" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,768.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms BB116301
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211656 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACCCTGGATTGTGGCTGTTCCTGGTTTCCTTATGCTTGACCAAAGAACTGGCAGAATCGGGACAGA
AGATGTCCTATGGAAAGCCATGTGGGAGCCGAGATTGCCATGGAGTCTGTACATGCTTTCCTGAGAAAGG
AGCAAGAGGGCACCCTGGACCAATTGGAACTCAAGGTCCAGCCGGTCCGGAAGGGTTTACTGGTCCAACT
GGTTTATCAGGTTTGAAAGGAGAAAGAGGCTCCCCAGGCCCTCTGGGACCATATGGATCAAAAGGAGATA
AGGGTCCCATTGGAGTTCCTGGCTTTGTTGGCATCAGTGGTATTCCGGGCCACCCTGGACAGCCAGGTCC
AAGAGGTCCACCTGGCCCAGATGGCTGTAATGGAACTCAAGGAGCTGTTGGATTTCCAGGCCCTGATGGC
TACCCTGGGATTTTAGGACCACCTGGGCTGCCTGGTCACAAAGGTGCCAAAGGAGAGCCTGTTCCTTTTC
AAGGCAGCATTACAGGAATAAGGGGAGATCCTGGGCTGCCTGGACTAGATGGAATCCCTGGTCCATCAGG
ATCCTCCGGCTCTCCTGGAGATGCAGGACCCATAGGACCACCAGGCTTACAAGGTCCCCCAGGCCTCCCC
GGACTTCCCGGTCCTGAGGGAAATATGGGGTTAGGTTTCCAAGGAGAAAAAGGAGTCAAGGGGGATGTTG
GCCTCCCTGGCCCTGCAGGACCACCTCCATCTACTGGGGAACTAGAATTCATGGGATTCCCCAAAGGAAA
GAAAGGTTCCAAGGGTGAGCCAGGGCCTCCAGGTTTCCCAGGAATGAGTGGCCCTCCAGGTGTCCCGGAA
TTTGGATCTACTGGAGAAAAAGGAGAAAAGGGGATTCTTGGTTTGCCAGGACCTAGGGGTCCCATGGGTT
CAGAAGGAATACAAGGCCATCCAGGGAGACAGGGAAAGAAGGGGACTTCAGGTTTTCCTGGGATTAACGG
GTTCCCAGGAATTAAGGGTGAAAAGGGAGACATTGGTGTTCGAGGCCCAGACAGTTTCACTGATGCAGAT
GGCGCTGTGATCTCAGGTTATCCTGGAGACCCTGGTGTGCTGGGCCTTCCAGGCCTTAGAGGAGATGAAG
GCGTCCAAGGCCAACGTGGCCCTTCTGGAACCCCTGGCCTCCCATCATTAACAGGTCTTCCAGGTGCCCT
AGGGCCTCAGGGATTTCCTGGCCTGAAAGGAGACCAAGGAAACTCAGGACGTACCACCTTTGGAGAAGCT
GGCCTACCTGGCAGGGTTGGTTTACCAGGTTTACCAGGCCTGCCAGGCCCATCAGGCCCACCTGGTCGCA
CATTTGAGACTGGACATCTGTCCAACATAGAGCCTGGGTTCCCTGGTCTCCAAGGAGAACAAGGTCCAAA
AGGACATCAAGGCCTCAAAGGAGTAAAAGGAGACTCTGGTTTTTGTGCTTGTGAAGGTGGTGCCCCCAAC
ATTGGACCACATGGGGAACCAGGTCTGCCTGGGATACAAGGTCCCATTGGTCTACAGGGTTTTAAGGGGA
CTAAAGGAGATCCAGGCTCAAGGGGAGCATCTGGTCCTGCAGGGACGCCAGGGCTATTTGGACCTAGAGG
TCAGACTGGCCTCAAAGGAAAGAAAGGAGAACCAACTGTCAGTAGAGGATCAAAAATGTCAGGGGACAAA
GGTGACCCTGGTCCTCAGGGTACCCCAGGTTTGGCAGGAACTCCGGGCAAGGATGGAAGACCAGGTTTAC
CAGGCCTCCCAGGCATTCAGGGAGATGGTGGGTCTGGCTTCCCAGGTGAAAGAGGGTTACCAGGACTTCC
TGGTGAAAAAGGCCATGATGGTCCAATTGGACCACCAGGAATTGGGCTGCCAGGACCTCCTGGGCCCCGT
GGACTTCCTGGAGATAAAGGAGTAGATGGGTTACCAGGGCAACAAGGCCTCCGTGGAGCTCAAGGAGTCA
CCTTGCCTTGTATCATTCCTGGGTCATATGGTCCATCAGGATTTCCTGGAGCTCCTGGATTCCCAGGCTC
TAAGGGAGCTCGGGGCCTCCCTGGAATTCCAGGCAAGCCTGGCACTCACGGAAGCAAAGGAGGGCCTGGG
AGTCCAGGGTTAATGCATCTCCCAGGATTGCCAGGGTTTCCTGGAGTTCGTGGAGAGAAGGGATTGCCTG
GGTTTCCTGAGCTTCCTGGAAAAGATGGCTACCCTGGGAAAGCTGGCAGTCCAGGGTTACCAGGTTTCAA
GGGAGCTGCTGGTGACATATTTGGTGCTGAAAATGGTGCTTCGGGGGAGCAAGGCCTACAGGGATTGCCA
GGGGACAAAGGATTTCCTGGAGACTCTGGCCTTCCAGGACCCAAGGGTTTGAATGGGAAACCTGGTATGC
TAGGCCCCAAGGGTGAGCGGGGCAACCCTGGAACATCAGGACCACCAGGACAGCCAGGACCCTCAGGGTC
TACTGACCCATTTGGCATCAAGGGCACATCTGGATTCCCAGGAGCACCAGGCCTTCCAGGCATTTCAGGG
CACCCTGGAAAGAAAGGTCAAAGAGGAGACATAGGTCAGCCTGGATCAACTGGAAAAAGAGGTCTACCTG
GGATAAAAGGCCTTCCTGGTCCTCAAGGGTCACCTGGCTTCCTGGGGAGCCCAGGTTTGTCAGGCGTCAC
TGGGTTGCCGGGCATCGAAGGCCAAAAGGGTGAAAAGGGGTCCTCTGGACCAGTAGGATTTCCTGGGTTA
CCTGGTCTTCCTGGTCTTCCTGGAGCTGATGGATTAAAGGGATTTTCTGGGTCATTTGGAAAGGTGGGAC
AACCTGGACAGACTGGTACTTCCGGAGAAAAAGGAGACAGAGGGGATCCAGGGCCAGTTGGCATATCTAG
TCCAAGACCTCCAATGCTAAACCTTTGGTTCAAAGGAGAAAAAGGCTCTCGAGGTTCTGCTGGATCGGAT
GGATTTCCTGGGCCCAGAGGTGACAAAGGAGAGCCTGGCATCCCAGGGTTACCAGGTCCACCTGGAGCTA
CTGGACAAACCAATACCATCAAAGGGCTCAATGGGAGGCCAGGTTCTCCTGGATCTATGGGAAGAAGGGG
ATTACCTGGCCTGAAGGGGTCTCTTGGGATTGCAGGTTTCCCAGGAATGCCAGGGAAAAGCGGTTCACAG
GGGCTCACTGGGACTTCTGGCCTCCTGGGAGCAACTGGTATCCCAGGTTTAAAAGGAGATCAAGGCCAAA
CACTTGGCATTTCTGGAAGCCCAGGACCCAAGGGACAGCCTGGAGAATTAGGTTTTAAAGGTGTCAAAGG
AAAAGATGGACTAGTTGGTGATAGAGGCCACCCAGATTTATGCAGGGTGACAAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211656 protein sequence
Red=Cloning site Green=Tags(s)

MHPGLWLFLVSLCLTKELAESGQKMSYGKPCGSRDCHGVCTCFPEKGARGHPGPIGTQGPAGPEGFTGPT
GLSGLKGERGSPGPLGPYGSKGDKGPIGVPGFVGISGIPGHPGQPGPRGPPGPDGCNGTQGAVGFPGPDG
YPGILGPPGLPGHKGAKGEPVPFQGSITGIRGDPGLPGLDGIPGPSGSSGSPGDAGPIGPPGLQGPPGLP
GLPGPEGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPPGFPGMSGPPGVPE
FGSTGEKGEKGILGLPGPRGPMGSEGIQGHPGRQGKKGTSGFPGINGFPGIKGEKGDIGVRGPDSFTDAD
GAVISGYPGDPGVLGLPGLRGDEGVQGQRGPSGTPGLPSLTGLPGALGPQGFPGLKGDQGNSGRTTFGEA
GLPGRVGLPGLPGLPGPSGPPGRTFETGHLSNIEPGFPGLQGEQGPKGHQGLKGVKGDSGFCACEGGAPN
IGPHGEPGLPGIQGPIGLQGFKGTKGDPGSRGASGPAGTPGLFGPRGQTGLKGKKGEPTVSRGSKMSGDK
GDPGPQGTPGLAGTPGKDGRPGLPGLPGIQGDGGSGFPGERGLPGLPGEKGHDGPIGPPGIGLPGPPGPR
GLPGDKGVDGLPGQQGLRGAQGVTLPCIIPGSYGPSGFPGAPGFPGSKGARGLPGIPGKPGTHGSKGGPG
SPGLMHLPGLPGFPGVRGEKGLPGFPELPGKDGYPGKAGSPGLPGFKGAAGDIFGAENGASGEQGLQGLP
GDKGFPGDSGLPGPKGLNGKPGMLGPKGERGNPGTSGPPGQPGPSGSTDPFGIKGTSGFPGAPGLPGISG
HPGKKGQRGDIGQPGSTGKRGLPGIKGLPGPQGSPGFLGSPGLSGVTGLPGIEGQKGEKGSSGPVGFPGL
PGLPGLPGADGLKGFSGSFGKVGQPGQTGTSGEKGDRGDPGPVGISSPRPPMLNLWFKGEKGSRGSAGSD
GFPGPRGDKGEPGIPGLPGPPGATGQTNTIKGLNGRPGSPGSMGRRGLPGLKGSLGIAGFPGMPGKSGSQ
GLTGTSGLLGATGIPGLKGDQGQTLGISGSPGPKGQPGELGFKGVKGKDGLVGDRGHPDLCRVTN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057648
ORF Size 3345 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057648, AAH57648
RefSeq Size 3952 bp
RefSeq ORF 3347 bp
Locus ID 94216
MW 106.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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