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Slc12a6 (BC062099) Mouse Tagged ORF Clone
CAT＃: MR211642
- TrueORF®
Slc12a6 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732)
ORF Plasmid: tGFP
"BC062099" in other vectors (4)
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CNY 11,020.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9530023I19Rik; gaxp; KCC3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211642 representing BC062099
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCCACCAGAAGCCACCACCAAGATGTCCTCAGTTCGGTTCATGGTGACACCAACTAAGATTGATG ACATTCCAGGTTTGTCAGACACCAGCCCGGACCTCAGCTCTCGGTCTAGTTCTCGAGTAAGATTTAGCTC CCGAGAAAGTGTGCCAGAAACAAGCCGTAGTGAGCCTATGAGCGAACTGTCTGGGGCTACTACTTCTCTG GCAACTGTTGCCCTAGATCCTTCCAGTGACCGGACTTCTAATCCCCAGGATGTTACGGAGGATGACGGCC ATAAAAAAGCCCGAAATGCTTATCTCAATAATTCCAACTATGAAGAAGGAGACGAATATTTTGATAAAAA TTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCAAAGGTGTCTTCTCTCCTCAACCGCATGGCCAAC TATACAAATCTGACACAAGGAGCAAAGGAACATGAAGAGGCAGAGAACATCACTGAAGGGAAAAAGAAGC CTACCAAGAGCCCCCAAATGGGTACTTTCATGGGTGTCTACCTCCCATGTCTACAGAACATCTTTGGAGT GATCCTCTTCCTGCGTCTTACCTGGGTAGTGGGAACAGCTGGAATCCTTCAGGCCTTTGCAATTGTCCTC ATCTGCTGCTGCTGTACAATGTTAACTGCCATCTCCATGAGCGCCATCGCCACTAACGGAGTGGTGCCAG CTGGGGGCTCATACTTCATGATTTCCAGAGCCCTGGGCCCAGAGTTTGGCGGGGCTGTAGGCCTCTGCTT TTATCTTGGCACCACATTTGCAGCAGCCATGTATATTCTTGGTGCCATTGAAATCTTTCTGGTATACATT GTCCCCCGAGCTGCCATCTTTCGGAGTGACGATGCACTCAAGGAGTCAGCAGCTATGCTGAACAACATGC GCGTCTATGGTACAGCCTTCTTGGTCCTCATGGTCTTGGTGGTATTCATCGGCGTACGCTATGTGAATAA GTTTGCCTCACTCTTCCTGGCCTGTGTAATTGTGTCGATCTTGGCTATCTATGCTGGTGCCATCAAGTCT TCCTTTGCTCCACCACACTTCCCGGTCTGTATGCTGGGCAACCGTACCCTGTCATCAAGACACCTTGACA TTTGCTCTAAGACCAAGGAGGTTGACAACATGACAGTACCATCAAAGTTATGGGGATTCTTCTGCAACTC GAGTCAGTTCTTTAATGCCACCTGTGATGAGTACTTTGTTCACAATAACGTCATCTCAATCCAAGGCATT CCAGGGTTGGCTAGTGGTATCATTACTGAAAATCTTTGGAGTAATTATTTACCAAAGGGTGAGATAATTG AAAAGCCATCAGCCAAGTCATCTGATGTCTTAGGCAACTTAAACCATGAATATGTTCTTGCTGATATCAC CACCTCCTTCACTCTGCTGGTGGGGATCTTCTTTCCCTCGGTCACAGGTATCATGGCTGGGTCAAACAGA TCTGGAGATCTGAAAGATGCCCAGAAGTCTATTCCCATTGGGACCATCCTTGCCATCCTGACCACATCCT TTGTGTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATTGAAGGAGTCGTTCTCAGAGACAAATTTGG GGATGCTGTAAAAGGGAATTTGGTTGTAGGCACCTTATCCTGGCCATCCCCGTGGGTGATCGTTATTGGC TCCTTCTTTTCAACATGTGGGGCTGGGCTGCAGAGCCTCACAGGTGCGCCTCGGCTGCTGCAGGCTATCG CCAAGGATAACATCATACCTTTCCTTAGGGTTTTTGGTCACAGCAAAGCTAATGGGGAACCTACCTGGGC TTTACTTCTAACTGCTGCCATAGCAGAGCTGGGAATTCTCATCGCCTCCCTGGATCTCGTGGCCCCAATT CTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTGAACTTGGCTTGTGCCTTGCAAACATTGCTGCGAA CCCCCAACTGGAGGCCTCGATTCCGCTATTATCACTGGGCCCTCTCTTTCATGGGAATGAGTATCTGTCT AGCTCTGATGTTCATTTCTTCTTGGTATTATGCCATTGTAGCTATGGTAATAGCTGGCATGATCTACAAG TACATTGAATATCAAGGGGCTGAGAAAGAATGGGGGGATGGTATCCGTGGGCTGTCGCTCAGTGCAGCCC GCTTCGCTTTGCTCCGTCTAGAGGAAGGACCTCCTCACACTAAAAACTGGAGGCCTCAGCTGCTCGTCCT ACTGAAGCTGGATGAAGATTTACACGTCAAGCACCCTCGCCTCCTCACCTTTGCCTCCCAGCTCAAGGCA GGAAAGGGACTCACGATTGTGGGCTCTGTCATCGTGGGGAACTTCTTGGAGAACTATGGTGACGCGCTCG CGGCAGAGCAGACCATTAAGCACCTAATGGAGGCAGAAAAGGTAAAAGGATTCTGCCAATTGGTGGTGGC TGCCAAGCTGAAAGAGGGCATTTCACACCTCATCCAGTCCTGTGGCCTCGGAGGCATGAAACACAACACA GTGGTGATGGGCTGGCCCAATGGCTGGCGTCAGAGTGAAGATGCTCGCGCTTGGAAGACTTTCATTGGCA CAGTACGAGTGACAACTGCTGCCCATCTAGCCCTGCTGGTGGCTAAAAATGTCTCCTTCTTTCCCAGCAA TGTGGAGCAGTTTTCTGAGGGCAACATTGATGTGTGGTGGATTGTGCATGATGGGGGCATGCTCATGCTA TTACCGTTCCTGCTGAAACAGCACAAGGTTTGGCGGAAATGCAGCATACGGATCTTCACAGTAGCCCAAC TAGAAGACAACAGTATCCAGATGAAGAAGGATCTGGCCACCTTTCTGTACCACCTGCGCATTGAGGCAGA AGTGGAAGTGGTGGAGATGCACGACAGTGACATATCTGCCTATACATATGAGCGCACCCTGATGATGGAG CAGAGGTCCCAGATGCTTCGGCATATGCGGCTGTCCAAAACAGAGCGAGACAGGGAGGCACAGCTGGTGA AAGATCGAAACTCAATGCTACGCTTGACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAAACGTACCA GGAGAAGGTGCACATGACTTGGACCAAGGATAAATACATGGCATCCCGGGGGCAAAAGGTCAAGTCAATG GAAGGATTCCAGGACCTACTTAATATGCGTCCGGACCAGTCCAACGTGAGACGGATGCATACAGCAGTGA AGCTCAATGAAGTTATAGTCAACAAGTCTCATGAAGCAAAGCTGGTTTTGTTGAATATGCCAGGACCACC CCGGAACCCTGAAGGTGATGAAAACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211642 representing BC062099
Red=Cloning site Green=Tags(s) MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSL ATVALDPSSDRTSNPQDVTEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMAN YTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVL ICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYI VPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKS SFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGI PGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNR SGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIG SFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPI LSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYK YIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKA GKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNT VVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLML LPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMME QRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSM EGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC062099 |
| ORF Size | 3318 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC062099.1 |
| RefSeq Size | 4439 bp |
| RefSeq ORF | 3320 bp |
| Locus ID | 107723 |
| MW | 162.7 kDa |
| Gene Summary | Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223117 | Slc12a6 (untagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732), (10ug) |
CNY 12,920.00 |
|
| MG211642 | Slc12a6 (tGFP-tagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732) |
CNY 12,160.00 |
|
| MR211642L3 | Lenti ORF clone of Slc12a6 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732) |
CNY 12,920.00 |
|
| MR211642L4 | Lenti ORF clone of Slc12a6 (mGFP-tagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732) |
CNY 12,920.00 |
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