Setd1b (NM_001040398) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA516740; BC035291; KMT2G; mKIAA1076
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR211620 representing NM_001040398 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACAGTCACCCCCACCACCACCACCAGCAGCCCCCGCCGCAGCCCGGCCCTTCGGGCGAGAGGA GGAACCACCATTGGAGAAGTTACAAGTTGATGATTGACCCGGCTCTGAAAAAGGGGCACCATAAACTGTA CCGCTACGATGGGCAGCATTTCAGCCTGGCCATGTCCAGCAACCGCCCGGTGGAGATCGTGGAAGATCCC CGGGTGGTCGGAATCTGGACCAAAAACAAGGAGCTGGAGCTGTCTGTGCCCAAATTCAAGATCGATGAGT TCTACGTGGGCCCTGTGCCTCCAAAGCAGGTGACATTTGCCAAGCTGAATGACAACGTCCGTGAAAACTT CCTAAGGGACATGTGCAAAAAGTATGGGGAGGTGGAGGAGGTGGAGATCTTGTATAACCCGAAGACCAAG AAACACCTGGGCATTGCCAAGGTGGTCTTTGCCACAGTCAGGGGCGCCAAGGAGGCCGTCCAACACCTGC ACAGCACTTCTGTCATGGGCAACATTATCCACGTGGAGCTGGACACCAAAGGGGAAACTCGCATGCGCTT CTACGAGCTGTTGGTCACCGGCCGGTACACCCCTCAGACCCTCCCTGTTGGTGAGCTGGATGCTATTTCT CCGATTGTGAGCGAGACCCTGCAGCTATCAGATGCTCTGAAGCGCCTCAAAGATGGCAGCCTGTCTGCAG GCTGCGGCTCTGGCTCCTCGTCAGTCACCCCCAATAGCGGTGGGACCCCCTTCTCCCAAGACACAGCTTA CTCCAGCTGCCGCCTGGACACACCCAACTCCTATGGACAAGGCACCCCAATCACGCCTCGCCTGGGCACC CCTTTCTCCCAGGATTCCAGCTACTCCAGCCGTCAGCCCACACCCTCCTATCTCTTCAGCCAGGACCCCA CAGCCACATTCAAGGCGAGGCGGCACGAGAGCAAGTTCACAGATGCCTACAACCGCCGCCACGAACATCA TTATGTCCACAACTCAGCGGTGGCTGGAGCCACAGCTCCTTTCCGAGGCTCCTCGGACCTCTCTTTTGGG ACAGTTGGCAGCAGCGGGACTCCCTTCAAGGCTCAGTCGCAGGATGCCACCACGTTTGCCCATACTCCAC CTCCTGCCCAAACAGCCACGGCTTCTGGATTCAAATCAGCTTTCTCTCCATATCAGACTCCAGCACCCCC CTTCCCGCCACCCCCTGAGGAGCCCACCGCCACAGCAGCCTTTGGGAGCCGCGACAGTGGGGAGTTCCGG AGGGCACCTGCGCCCCCGCCCCTGCCACCCGCTGAGCCTCCAGCCAAGGAGAAGCCAGGCACCCCTCCTG GTCCCCCGCCCCCTGACAGCAACAGCATGGAGCTGGGTGGTCGGCCTACCTTTGGCTGGAGTCCTGAGCC CTGTGACAGCCCTGGCACCCCTACTCTGGAGTCCTCTCCAGCCGGGCCAGAGAAGCCCCATGACAGCCTG GACTCCCGCATTGAGATGCTGCTGAAGGAGCAACGCACCAAGCTGCCCTTCCTTCGTGAGCAGGACTCAG ACACCGAGATACAGATGGAAGGCAGCCCCATCTCCTCCTCCTCCTCCCAACTCTCCCCGCTCAGCCACTT TGGCACTAACTCGCAGCCCGGCTTCCGCGGTCCCTCGCCACCCTCCTCGCGCCCCTCCAGCACAGGTCTA GAGGACATCAGTCCCACACCACTGCCTGACTCTGATGAAGATGAGGACTTGGGCCTGGGCCTGGGGCCTC GGCCCCCACCTGAGCCAGGCCCCCCAGACCCTATGGGTCTGCTAGGCCAGACAGCCGAGGTGGATTTGGA CCTGGCAGGAGACAGGACCCCCACCTCAGAGCGGATGGATGAGGGCCAGCAGTCCTCAGGAGAGGACATG GAAATCTCAGATGATGAGATGCCCTCGGCCCCCATCACCAGCGCTGACTGCCCCAAGCCCATGGTGGTGA CCCCAGGGGCCGGGGCTGTGGCAGCCCCCAACGTGCTGGCTCCAAACCTGCCCCTGCCCCCACCCCCTGG CTTCCCTCCATTACCCCCGCCCCCTCCTCCACCCCCACCACAACCTGGCTTCCCCATGCCCCCACCTCTG CCTCCACCACCACCACCACCACCACCAGCCCATCCTGCAGTGACAGTGCCCCCGCCACCCTTGCCAGCGC CCCCGGGTGTCCCGCCCCCACCCATCCTTCCGCCGCTACCTCCCTTCCCACCGGGACTGTTTCCTGTGAT GCAGGTGGACATGAGCCATGTGCTGGGTGGCCAGTGGGGTGGCATGCCCATGTCCTTCCAGATGCAAACA CAGATGTTAAGCCGGCTCATGACAGGCCAGGGGGCTTGTCCCTACCCACCTTTCATGGCCGCTGCGGCTG CCGCCGCTTCTGCTGGACTGCAGTTTGTCAACTTGCCCCCCTACCGGAGCCCATTCTCCCTGAGCAACAG TGGCCCAGGACGTGGACAGCATTGGCCGCCCCTGCCCAAGTTTGACCCCTCCGTGCCACCACCAGGCTAC ATACCACGTCAGGAGGACCCGCACAAGGCCACGGTGGATGGGGTCTTGCTGGTAGTGCTCAAGGAACTTA AGGCCATCATGAAGCGTGATCTCAACCGCAAGATGGTAGAGGTGGTGGCCTTCCGGGCCTTTGACGAATG GTGGGACAAGAAGGAGCGGATGGCCAAGGCCTCACTGACCCCCGTGAAGTCCGGTGAGCACAAGGACGAG GACAGGCCAAAGCCCAAAGACCGAATCGCCTCCTGTCTGCTGGAGTCGTGGGGCAAGGGTGAGGGCCTGG GTTACGAGGGCCTAGGCCTGGGCATTGGACTGCGGGGCGCCATTCGCTTGCCCTCCTTCAAGGTCAAGAG GAAGGAGCCACCGGACACAGCCTCTTCTGGTGACCAGAAGCGGCTACGGCCTTCAACCTCTGTGGATGAG GAAGATGAAGAGTCAGAGCGGGAGCGGGACCGGGACATAGCTGATGCTCCCTGCGAACTCACCAAGCGGG ACCCCAAAAGCGTGGGTGTGCGACGCCGGCCAGGACGGCCCCTGGAGCTGGACAGCGGCGGGGAGGAGGA TGAGAAGGAGTCCTTGTCAGCGTCCTCCTCTTCCTCAGCATCCTCATCCTCTGGATCTTCTACCACCTCA CCCTCATCCTCCGCCTCAGACAAGGAAGAGGAGGACCGAGAAAGCACCGAGGAGGAGGAGGAGGAAGAGG AGGAGGAAGCAGAGGAAGAGGAGGAGGAAGGCCCCAGGAGCCGCATATCCTCACCCTCATCCTCCTCAAG CTCCGACAAGGATGACGAAGATGACAACGAAGCCGACAGCGATGGCCAGATTGATTCAGACATCGACGAT CAGGGTGCACCCCTGTCGGAGGCCAGCGAGAAAGACAATGGAGATTCTGAGGAGGAGGAGACAGAGAGCA TCACCACCTCAAAGGCCCCAGCTGAGTCGTCCTCATCCTCCAGTGAAAGTTCTGGATCTTCTGAGTTTGA ATCCAGTTCCGAGTCCGAGTCTTCATCTTCATCCTCAGAGGACGAGGAGGAGATGACCGTACCTGGGGTG GAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGAAAGAGACTGCAATGGCTGCAGCCACGGTAGTGGCTA TGGCAGAAGAGAGCATGCCTCCAGCGGGTGGCCAGGACTTTGAGCAGGACAGGGCAGAAGTGCCTCTGGG CCCACGAGGCCCCATGAGGGAGTCCTTGGGCACAGAGGAGGAAGTGGACATTGAGGCTGAGGACGAAGTC CCTGAGATGCAGGCTCCTGAGCTGGAGGAGCCTCCATTGCCCATGGGTGCTCGGAAGCTGGAAGGGAGCC CAGAGCCCCCAGAAGAGCCAGGCCCCAACACACAAGGGGACATGCTGCTCTCTCCAGAGCTCCCTGCCCG GGAGACGGAGGAGGCTCAACTCCCATCACCGCCTGAACATGGTCCAGAGTCAGACCTGGACATGGAGCCC GAGCCCCCTCCTATGCTCTCCTTGCCCTTGCAGCCGCCATTGCCGCCCCCTCGACTGCTCCGGCCACCCA GCCCACCGCCTGAGCCTGAGACCCCAGAGCCCCCCAAACCTCCCGTCCCCCTGGAGCCTCCCCCAGAAGA CCACCCTCCACGTACCCCAGGCCTCTGTGGCAGTCTGGCCAAGTCACAGAGCACAGAGACAGTGCCAGCC ACCCCAGGCGGGGAGCCCCCACTGTCAGGGAGCAGCAGTGGCCTGTCCCTGAGCTCCCCGCAGGTGCCTG GCAGCCCGTTCTCCTATCCATCCCCGTCCCCCGGCTTGAGCAGTGGGGGACTTCCACGGACACCTGGTCG GGACTTCAGCTTCACACCTACTTTCCCTGAGCCCAGTGGACCCTTGCTTCTGCCTGTCTGTCCCCTCCCA ACCGGTCGGCGAGATGAGCGTACCGGCCCCCTGGCCTCCCCAGTGCTCCTGGAAACTGGCCTGCCCCTCC CACTTCCCCTGCCCCTGCCCTTACCTCTGGCATTGCCTGTCCCGGTTCTGAGGGCCCAGCCTCGGCCACC TCCCCAGCTGCCTCCCCTACTGCCCGCTACCCTAGCTCCCTGCCCAACCCCCATCAAGAGGAAGCCAGGC CGGCCCCGGAGATCCCCACCGTCCATGCTGTCCTTGGATGGGCCTTTGGTCCGGCCACCGCCAGGACCTG CCCTTGGGAGGGACCTTCTCCTCCTGCCAGGCCAGCCACCGGCCCCCATTTTCCCCAGCGCACATGACCC TCGGGCTGTGACCTTGGACTTCCGGAACACGGGGATCCCGGCCCCCCCACCGCCCCTCCCACCCCAGCCT CCCCCACCTCCACCCCCACCACCCGTTGAATCCACCAAGCTACCCTTCAAGGAGCTGGACAACCAGTGGC CCTCTGAAGCTATCCCCCCAGGACCTCGCCGAGATGAGGTCACTGAAGAGTACGTGGACCTGGCCAAGGT GCGGGGACCGTGGCGGCGGCCACCCAAGAAGCGGCACGAAGACCTGGTGGCCCCATCGGCCTCACCTGAA CCCTCACCACCCCAGCCTCTCTTCCGGCCCCGCTCAGAGTTTGAGGAGATGACCATCTTGTACGACATCT GGAACGGTGGCATCGACGAGGAGGACATCCGCTTCCTGTGCGTCACCTACGAGCGCCTGCTGCAGCAGGA CAATGGCATGGACTGGCTGAATGACACACTCTGGGTCTACCATCCCTCCACCAGCCTCTCCTCAGCCAAG AAAAAGAAACGGGAGGACGGTATCCGTGAGCACGTGACAGGCTGCGCCCGAAGCGAGGGCTTCTACACCA TCGACAAGAAGGACAAGCTGAGATACCTCAACAGCAGCCGTGCCAGCACAGACGAGCCCCCCATGGACAC CCAGGGTATGAGTATCCCAGCCCAGCCCCATGCCTCTACTCGGGCAGGCTCAGAGCGGCGGTCTGAGCAG AGGCGCCTCTTGTCCTCCTTCACCGGCAGCTGTGACAGCGACCTGCTCAAGTTCAACCAGCTCAAGTTTC GAAAGAAGAAGCTCAAATTCTGTAAGAGCCACATTCACGACTGGGGCCTGTTTGCCATGGAGCCAATTGC TGCTGACGAGATGGTCATCGAGTACGTGGGCCAGAACATCCGCCAGGTGATTGCGGACATGAGGGAGAAA CGGTATGAGGATGAAGGCATCGGTAGCAGCTACATGTTCAGAGTAGACCATGACACCATCATCGACGCCA CCAAGTGCGGCAACTTTGCACGCTTCATTAACCACAGCTGCAATCCCAACTGCTACGCCAAGGTGATCAC GGTGGAGTCCCAGAAGAAGATCGTCATCTACTCTAAGCAGCACATCAACGTCAACGAGGAGATCACGTAC GACTACAAGTTCCCGATCGAAGATGTCAAGATCCCCTGCCTCTGTGGCTCCGAGAACTGCCGGGGGACCC TCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR211620 representing NM_001040398 Red=Cloning site Green=Tags(s) MENSHPHHHHQQPPPQPGPSGERRNHHWRSYKLMIDPALKKGHHKLYRYDGQHFSLAMSSNRPVEIVEDP RVVGIWTKNKELELSVPKFKIDEFYVGPVPPKQVTFAKLNDNVRENFLRDMCKKYGEVEEVEILYNPKTK KHLGIAKVVFATVRGAKEAVQHLHSTSVMGNIIHVELDTKGETRMRFYELLVTGRYTPQTLPVGELDAIS PIVSETLQLSDALKRLKDGSLSAGCGSGSSSVTPNSGGTPFSQDTAYSSCRLDTPNSYGQGTPITPRLGT PFSQDSSYSSRQPTPSYLFSQDPTATFKARRHESKFTDAYNRRHEHHYVHNSAVAGATAPFRGSSDLSFG TVGSSGTPFKAQSQDATTFAHTPPPAQTATASGFKSAFSPYQTPAPPFPPPPEEPTATAAFGSRDSGEFR RAPAPPPLPPAEPPAKEKPGTPPGPPPPDSNSMELGGRPTFGWSPEPCDSPGTPTLESSPAGPEKPHDSL DSRIEMLLKEQRTKLPFLREQDSDTEIQMEGSPISSSSSQLSPLSHFGTNSQPGFRGPSPPSSRPSSTGL EDISPTPLPDSDEDEDLGLGLGPRPPPEPGPPDPMGLLGQTAEVDLDLAGDRTPTSERMDEGQQSSGEDM EISDDEMPSAPITSADCPKPMVVTPGAGAVAAPNVLAPNLPLPPPPGFPPLPPPPPPPPPQPGFPMPPPL PPPPPPPPPAHPAVTVPPPPLPAPPGVPPPPILPPLPPFPPGLFPVMQVDMSHVLGGQWGGMPMSFQMQT QMLSRLMTGQGACPYPPFMAAAAAAASAGLQFVNLPPYRSPFSLSNSGPGRGQHWPPLPKFDPSVPPPGY IPRQEDPHKATVDGVLLVVLKELKAIMKRDLNRKMVEVVAFRAFDEWWDKKERMAKASLTPVKSGEHKDE DRPKPKDRIASCLLESWGKGEGLGYEGLGLGIGLRGAIRLPSFKVKRKEPPDTASSGDQKRLRPSTSVDE EDEESERERDRDIADAPCELTKRDPKSVGVRRRPGRPLELDSGGEEDEKESLSASSSSSASSSSGSSTTS PSSSASDKEEEDRESTEEEEEEEEEEAEEEEEEGPRSRISSPSSSSSSDKDDEDDNEADSDGQIDSDIDD QGAPLSEASEKDNGDSEEEETESITTSKAPAESSSSSSESSGSSEFESSSESESSSSSSEDEEEMTVPGV EEEEEEEEEEEKETAMAAATVVAMAEESMPPAGGQDFEQDRAEVPLGPRGPMRESLGTEEEVDIEAEDEV PEMQAPELEEPPLPMGARKLEGSPEPPEEPGPNTQGDMLLSPELPARETEEAQLPSPPEHGPESDLDMEP EPPPMLSLPLQPPLPPPRLLRPPSPPPEPETPEPPKPPVPLEPPPEDHPPRTPGLCGSLAKSQSTETVPA TPGGEPPLSGSSSGLSLSSPQVPGSPFSYPSPSPGLSSGGLPRTPGRDFSFTPTFPEPSGPLLLPVCPLP TGRRDERTGPLASPVLLETGLPLPLPLPLPLPLALPVPVLRAQPRPPPQLPPLLPATLAPCPTPIKRKPG RPRRSPPSMLSLDGPLVRPPPGPALGRDLLLLPGQPPAPIFPSAHDPRAVTLDFRNTGIPAPPPPLPPQP PPPPPPPPVESTKLPFKELDNQWPSEAIPPGPRRDEVTEEYVDLAKVRGPWRRPPKKRHEDLVAPSASPE PSPPQPLFRPRSEFEEMTILYDIWNGGIDEEDIRFLCVTYERLLQQDNGMDWLNDTLWVYHPSTSLSSAK KKKREDGIREHVTGCARSEGFYTIDKKDKLRYLNSSRASTDEPPMDTQGMSIPAQPHASTRAGSERRSEQ RRLLSSFTGSCDSDLLKFNQLKFRKKKLKFCKSHIHDWGLFAMEPIAADEMVIEYVGQNIRQVIADMREK RYEDEGIGSSYMFRVDHDTIIDATKCGNFARFINHSCNPNCYAKVITVESQKKIVIYSKQHINVNEEITY DYKFPIEDVKIPCLCGSENCRGTLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001040398
ORF Size	5955 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001040398.2, NP_001035488.2
RefSeq Size	8868 bp
RefSeq ORF	5958 bp
Locus ID	208043
UniProt ID	Q8CFT2
MW	215.4 kDa
Gene Summary	Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.