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Esyt1 (NM_011843) Mouse Tagged ORF Clone
CAT＃: MR211619
- TrueORF®
Esyt1 (Myc-DDK-tagged) - Mouse extended synaptotagmin-like protein 1 (Esyt1)
ORF Plasmid: tGFP
"NM_011843" in other vectors (4)
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CNY 7,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fam62a; Mbc2; vp115 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211619 representing NM_011843
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCACTCCCCTGAAGAGGGCGCCAGCCCCGAGCCGTCAGGGCAGCCCCCTGCCACGGACTCCACGC GGGACGGGGGCTCCGGGGTCCCACCTGCCGGCCCAGGTGCGGCGAGCGAGGCCCTGGCGGTGCTGACTTC CTTCGGGCGCCGCTTGTTGGTGCTGGTGCCGGTGTACCTGGCAGGGGCAGCGGGTCTTAGCGTAGGTTTC GTGCTTTTCGGCCTCGCCCTGTACTTGGGCTGGCGCCGGGTCCGCGATGGGAAAGAACGGAGCCTGAGGG CAGCGAGGCAGCTGCTGGATGACGAGGAGCGGATCACCGCAGAGACGCTTTATATGAGCCACCGGGAACT ACCTGCCTGGGTCAGCTTCCCAGATGTGGAAAAGGCCGAATGGCTGAACAAGATCGTGGCTCAGGTGTGG CCCTTCCTAGGCCAGTATATGGAGAAGCTTCTGGCGGAGACAGTGGCCCCAGCTGTCCGGGGAGCTAACC CTCATCTGCAGACATTCACATTCACACGTGTGGAGCTGGGTGAAAAGCCATTACGAATCATTGGCGTCAA AGTTCACCCTAGTCAGAGGAAAGATCAGATTCTGTTGGACTTGAATGTCAGCTATGTAGGTGATGTACAG ATTGACGTGGAGGTGAAGAAATATTTCTGCAAAGCTGGAGTCAAGGGCATGCAGCTCCATGGTGTCTTGC GAGTGATTCTTGAGCCACTCACAGGGGACCTTCCTATCGTGGGGGCTGTGTCCATGTTCTTTATCAAACG CCCGACGCTTGACATCAACTGGACAGGGATGACCAACCTGCTAGATATCCCAGGACTTAGCTCACTCTCT GACACCATGATCATGGACTCCATTGCTGCCTTCCTCGTGCTCCCTAACCGACTGTTGGTGCCCCTTGTGC CCGACCTTCAAGATGTGGCCCAGCTGCGGTCCCCACTACCCAGGGGCATTATCCGGATTCACCTGCTGGC AGCCCGAGGTCTGAGTTCCAAGGACAAGTATGTGAAAGGCCTGATTGAGGGCAAATCGGATCCCTACGCG CTCGTCCGTGTGGGCACCCAGACGTTCTGCAGCCGTGTCATAGATGAGGAGCTCAACCCTCACTGGGGAG AGACATATGAGGTGATAGTCCACGAGGTTCCAGGACAGGAGATCGAGGTGGAGGTATTTGACAAAGATCC AGATAAAGATGATTTTCTGGGAAGAATGAAGCTGGACGTGGGGAAGGTATTACAGGCTGGAGTCCTGGAT AATTGGTACCCTCTGCAAGGCGGGCAAGGCCAAGTTCACTTGAGACTAGAATGGCTATCACTCCTGCCAG ATGCAGAGAAGCTGGATCAGGTCCTGCAGTGGAATCGGGGCATCACTTCTCGGCCAGAGCCCCCATCGGC TGCCATCCTCGTTGTCTACTTGGACCGAGCCCAGGACCTTCCTCTGAAGAAAGGCAACAAGGAACCCAAC CCCATGGTGCAACTATCAGTTCAGGATGTGACCCGGGAGAGCAAGGCTACCTACAGCACCAACAGCCCGG TGTGGGAAGAGGCTTTCCGGTTTTTCCTGCAGGACCCTCGAAGCCAGGAGCTTGATGTACAGGTGAAGGA CGACTCCCGAGCCCTGACTTTAGGGGCCCTGACTCTCCCTCTTGCGCGCCTGTTGACTGCCTCTGAACTC ACCCTGGACCAGTGGTTCCAGCTCAGCAGCTCAGGCCCAAACTCCAGGCTCTACATGAAACTGGTCATGC GGATCTTATACTTAGATTACTCGGAAATCCGCTTCCCCACTGTGCCTGGTGCCCAGGACTGGGACCGTGA GAGCCTAGAGACAGGCAGCAGTGTGGATGCCCCACCTCGGCCCTATCACACAACCCCTAACAGCCACTTT GGGACTGAGAATGTTCTTCGGATCCATGTATTAGAAGCGCAGGACCTAATTGCCAAAGACCGTTTCTTGG GAGGCCTGGTGAAGGGCAAATCAGACCCCTACGTCAAACTAAAGGTGGCAGGAAAAAGTTTCCGGACCCA TGTTGTTCGGGAAGATCTCAATCCCCGCTGGAATGAGGTTTTTGAGGTGATTGTCACATCAATCCCTGGC CAAGAGCTTGAGATTGAGGTCTTTGATAAGGACTTGGACAAGGATGACTTTTTGGGCAGGTATAAAGTGA GTCTCACCACAGTGCTCAACAGTGGCTTTCTTGATGAGTGGCTGACCCTGGAGGATGTCCCATCCGGGCG CCTGCACTTGCGCCTGGAGCGTCTGACCCCTAGACCGACTGCTGCTGAGTTAGAGGAGGTGCTGCAGGTG AACAGCCTGATCCAGACTCAGAAGAGTTCAGAGCTGGCAGCAGCCCTGCTGTCCGTCTTCCTGGAGCGGG CAGAAGATCTGCCGCTCCGAAAAGGTACCAAGCCTCCCAGCCCTTACGCTACTATCACCGTGGGAGAGAC TTCCCATAAAACCAAGACTGTCTCCCAATCTTCGGCCCCTGTCTGGGAGGAGAGTGCCTCTTTTCTCATC AGGAAGCCACACGCTGAGAGCCTGGAGCTGCAGGTTCGGGGTGAAGGGACCGGCACCCTGGGCTCTGTAT CCCTTCCTCTCTCTGAGCTCCTCCAGGAGGACCAGCTCTGCCTGGACCACTGGTTTGCACTCAGTGGTCA GGGCCAGGTGCTGATGAGAGCGCAGCTTGGGATCCTGGTATCTCAGCACTCGGGAGTGGAAGCCCACAGC CACAGCTACAGCCACAGTCACAGCTCCTCCTCGCTCAATGACGAACCTGAGGCCTTAGGGGGACCCACTC ACCCTGCCTCCCCGGTCCTAGAGGTCAGGCATCGCCTAACACATGGTGACAGTCCCTCTGAGGCTCCCGT TGGGCCCCTGGGCCAGGTGAAGCTGACAGTGTGGTACCACAGCGACGAACAAAAGCTGATCAGCATTATT CACAGCTGCCGGGCCCTTCGACAAAATGGACGTGATCTCCCAGACCCCTATGTGTCAGTGTTGCTGCTCC CAGACAAGAACCGAAGCACCAAGAGGAAGACCCCGCAGAAGAAGAGGACCCTCAATCCTGAGTTCAATGA GCGGTTTGAGTGGGATCTGCCCCTGGATGGGACTCTCAGGCGGAAGCTCGATGTCTCTGTGAAGTCAAAC TCCTCCTTCATGTCACGAGAGCGTGAGCTTCTGGGGAAGGTGCAGCTGGATCTAGCAGAGATAGACCTTT CCCAGGGTGCAGCCCAGTGGTATGACCTGATGGATGACAGAGATAAGGGCGGTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211619 representing NM_011843
Red=Cloning site Green=Tags(s) MEHSPEEGASPEPSGQPPATDSTRDGGSGVPPAGPGAASEALAVLTSFGRRLLVLVPVYLAGAAGLSVGF VLFGLALYLGWRRVRDGKERSLRAARQLLDDEERITAETLYMSHRELPAWVSFPDVEKAEWLNKIVAQVW PFLGQYMEKLLAETVAPAVRGANPHLQTFTFTRVELGEKPLRIIGVKVHPSQRKDQILLDLNVSYVGDVQ IDVEVKKYFCKAGVKGMQLHGVLRVILEPLTGDLPIVGAVSMFFIKRPTLDINWTGMTNLLDIPGLSSLS DTMIMDSIAAFLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSKDKYVKGLIEGKSDPYA LVRVGTQTFCSRVIDEELNPHWGETYEVIVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDVGKVLQAGVLD NWYPLQGGQGQVHLRLEWLSLLPDAEKLDQVLQWNRGITSRPEPPSAAILVVYLDRAQDLPLKKGNKEPN PMVQLSVQDVTRESKATYSTNSPVWEEAFRFFLQDPRSQELDVQVKDDSRALTLGALTLPLARLLTASEL TLDQWFQLSSSGPNSRLYMKLVMRILYLDYSEIRFPTVPGAQDWDRESLETGSSVDAPPRPYHTTPNSHF GTENVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKVAGKSFRTHVVREDLNPRWNEVFEVIVTSIPG QELEIEVFDKDLDKDDFLGRYKVSLTTVLNSGFLDEWLTLEDVPSGRLHLRLERLTPRPTAAELEEVLQV NSLIQTQKSSELAAALLSVFLERAEDLPLRKGTKPPSPYATITVGETSHKTKTVSQSSAPVWEESASFLI RKPHAESLELQVRGEGTGTLGSVSLPLSELLQEDQLCLDHWFALSGQGQVLMRAQLGILVSQHSGVEAHS HSYSHSHSSSSLNDEPEALGGPTHPASPVLEVRHRLTHGDSPSEAPVGPLGQVKLTVWYHSDEQKLISII HSCRALRQNGRDLPDPYVSVLLLPDKNRSTKRKTPQKKRTLNPEFNERFEWDLPLDGTLRRKLDVSVKSN SSFMSRERELLGKVQLDLAEIDLSQGAAQWYDLMDDRDKGGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011843 |
| ORF Size | 3276 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011843.2, NP_035973.1 |
| RefSeq Size | 3687 bp |
| RefSeq ORF | 3279 bp |
| Locus ID | 23943 |
| UniProt ID | Q3U7R1 |
| MW | 122 kDa |
| Gene Summary | Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200986 | Esyt1 (untagged) - Mouse extended synaptotagmin-like protein 1 (Esyt1), (10ug) |
CNY 7,608.00 |
|
| MG211619 | Esyt1 (tGFP-tagged) - Mouse membrane bound C2 domain containing protein (Mbc2) |
CNY 9,220.00 |
|
| MR211619L3 | Lenti ORF clone of Esyt1 (Myc-DDK-tagged) - Mouse extended synaptotagmin-like protein 1 (Esyt1) |
CNY 10,260.00 |
|
| MR211619L4 | Lenti ORF clone of Esyt1 (mGFP-tagged) - Mouse extended synaptotagmin-like protein 1 (Esyt1) |
CNY 10,260.00 |
