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Xpo1 (NM_001035226) Mouse Tagged ORF Clone
CAT＃: MR211584
- TrueORF®
Xpo1 (Myc-DDK-tagged) - Mouse exportin 1, CRM1 homolog (yeast) (Xpo1), transcript variant 2
ORF Plasmid: tGFP
"NM_001035226" in other vectors (4)
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CNY 11,184.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA420417; Crm1; Exp1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211584 representing NM_001035226
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCAATTATGACAATGTTAGCAGACCATGCAGCTCGTCAGCTGCTTGATTTCAGCCAAAAACTGG ATATCAACCTTTTAGACAACGTGGTGAATTGCTTATACCATGGAGAAGGAGCCCAGCAAAGAATGGCTCA AGAAGTACTGACACATTTAAAGGAGCATCCTGATGCTTGGACACGGGTCGACACAATATTGGAGTTTTCG CAGAACATGAACACGAAATACTATGGACTACAGATTTTGGAAAATGTGATCAAAACAAGGTGGAAGATCC TTCCAAGGAACCAGTGTGAAGGAATAAAAAAATATGTTGTTGGTCTCATTATCAAGACATCCTCTGACCC AACTTGTGTAGAGAAAGAAAAGGTGTATATTGGGAAATTGAATATGATTCTTGTTCAGATACTTAAGCAA GAGTGGCCTAAGCATTGGCCAACTTTTATCAGTGACATTGTTGGAGCGAGTAGGACCAGTGAAAGTCTAT GTCAAAATAATATGGTGATTCTTAAACTCTTGAGTGAAGAAGTGTTTGATTTCTCTAGTGGACAGATAAC CCAAGTTAAAGCAAAGCATTTAAAAGACAGCATGTGCAATGAGTTTTCACAAATATTTCAGCTTTGTCAG TTTGTGATGGAAAATTCCCAAAATGCTCCCCTTGTTCATGCTACATTAGAAACACTGCTGAGATTTCTGA ACTGGATTCCACTGGGATACATCTTTGAGACCAAGTTAATCAGCACATTAATTTACAAGTTCCTGAATGT TCCAATGTTCCGAAATGTGTCTCTAAAGTGCCTCACTGAGATTGCTGGTGTGAGTGTAAGCCAATATGAG GAACAATTTGAAACTCTGTTTACACTTACAATGATGCAGCTAAAACAGATGCTTCCTTTAAACACTAATA TCCGACTTGCATACTCGAATGGGAAAGATGATGAACAGAACTTTATTCAAAATCTCAGCCTGTTTCTCTG TACCTTTCTAAAAGAGCACGGTCAACTTCTAGAAAAACGACTGAATCTCAGGGAAGCACTCATGGAGGCT CTTCATTATATGTTGTTGGTGTCTGAAGTGGAAGAAACTGAAATTTTTAAAATCTGCCTTGAGTACTGGA ATCATTTGGCAGCTGAGCTCTACAGAGAGAGTCCATTTTCTACATCTGCTTCTCCCTTGTTATCTGGAAG TCAACACTTTGATATTCCTCCTAGGAGACAGCTGTACTTGACCGTGTTATCAAAGGTCCGTTTATTGATG GTTAGTCGCATGGCTAAACCAGAGGAGGTACTGGTTGTAGAAAATGATCAGGGAGAAGTTGTAAGAGAGT TCATGAAGGATACTGATTCCATAAATTTATATAAGAATATGAGAGAAACATTAGTTTATCTTACTCATCT GGATTATGTAGATACGGAAATAATAATGACTAAGAAACTTCAAAATCAAGTGAATGGTACAGAATGGTCA TGGAAGAATTTGAATACGTTGTGTTGGGCAATAGGCTCCATCAGTGGCGCAATGCATGAAGAGGATGAGA AGCGGTTTCTTGTTACTGTTATAAAGGATCTTTTAGGATTATGTGAGCAGAAAAGAGGCAAAGATAATAA AGCTATTATTGCATCGAATATCATGTACATAGTAGGTCAATACCCAAGATTTTTGAGAGCACACTGGAAA TTTTTGAAGACAGTAGTAAACAAGCTATTCGAATTCATGCATGAGACCCATGATGGAGTCCAGGATATGG CTTGTGACACATTTATTAAAATAGCTCAAAAATGCCGTAGGCATTTTGTTCAGGTTCAAGTTGGAGAAGT AATGCCGTTCATTGATGAGATTTTAAACAACATAAATACTATAATTTGTGATCTTCAGCCTCAGCAGGTT CATACATTTTATGAAGCTGTGGGGTACATGATTGGTGCACAGACAGACCAGACAGTTCAAGAGCATTTGA TAGAAAAATACATGCTACTCCCTAATCAAGTGTGGGACAGTATAATTCAGCAGGCTACCAAAAATGTGGA TATCCTGAAAGATCCTGAAACAGTCAAGCAGCTGGGTAGCATATTAAAAACAAATGTGAGAGCCTGCAAA GCTGTTGGTCATCCCTTTGTAATTCAGCTTGGCAGAATTTATTTAGACATGCTTAATGTATATAAGTGCC TCAGTGAAAATATTTCTGCAGCTATTCAAGCTAATGGTGAGATGGTTACAAAGCAACCATTGATTAGAAG TATGCGGACAGTAAAAAGGGAAACTTTAAAGTTAATATCTGGTTGGGTGAGCCGTTCCAATGATCCTCAG ATGGTAGCTGAAAACTTTGTTCCTCCTTTGTTGGATGCAGTTCTTATTGATTACCAGAGAAATGTACCAG CTGCTAGAGAACCAGAAGTGCTTAGTACTATGGCCATTATTGTCAACAAATTAGGTGGACATATAACAGC TGAAATACCTCAAATATTCGATGCAGTTTTTGAGTGCACATTGAATATGATAAATAAGGATTTTGAAGAG TATCCAGAACACAGAACAAACTTTTTCTTATTACTTCAAGCTGTCAATTCTCACTGTTTCCCAGCATTCC TAGCTATTCCACCTGCACAGTTCAAGCTGGTTTTGGATTCTATTATTTGGGCTTTTAAACATACTATGAG GAATGTTGCAGATACAGGCTTACAGATACTTTTTACACTTTTACAAAATGTTGCACAAGAGGAAGCTGCC GCACAGAGTTTTTATCAAACTTATTTTTGTGATATTCTTCAGCATATTTTCTCTGTTGTAACAGACACTT CACATACTGCAGGTCTAACAATGCATGCATCAATTCTTGCATATATGTTTAATTTGGTTGAAGAAGGAAA AATAAGTACACCACTAAATCCTGGAAATCCAGTTAACAACCAAATGTTTATTCAAGACTATGTGGCTAAT CTCCTTAAATCTGCATTCCCTCATCTACAAGATGCTCAAGTAAAGCTCTTTGTAACAGGACTTTTCAGCT TAAATCAGGATATTCCTGCTTTCAAAGAACACCTAAGGGATTTCTTAGTTCAAATAAAGGAGTTTGCAGG TGAAGATACATCGGATCTATTTTTGGAAGAAAGAGAAACAGCCCTTCGACAGGCTCAGGAGGAGAAACAT AAACTTCAAATGTCTGTACCTGGGATTCTTAATCCACACGAAATTCCAGAAGAAATGTGTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211584 representing NM_001035226
Red=Cloning site Green=Tags(s) MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFS QNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQ EWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKAKHLKDSMCNEFSQIFQLCQ FVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYE EQFETLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHGQLLEKRLNLREALMEA LHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDIPPRRQLYLTVLSKVRLLM VSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTEIIMTKKLQNQVNGTEWS WKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWK FLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQV HTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACK AVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQ MVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEE YPEHRTNFFLLLQAVNSHCFPAFLAIPPAQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAA AQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTPLNPGNPVNNQMFIQDYVAN LLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEERETALRQAQEEKH KLQMSVPGILNPHEIPEEMCD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001035226 |
| ORF Size | 3213 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001035226.1, NP_001030303.1 |
| RefSeq Size | 4906 bp |
| RefSeq ORF | 3216 bp |
| Locus ID | 103573 |
| UniProt ID | Q6P5F9 |
| MW | 123.5 kDa |
| Gene Summary | Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223439 | Xpo1 (untagged) - Mouse exportin 1, CRM1 homolog (yeast) (Xpo1), transcript variant 2, (10ug) |
CNY 13,780.00 |
|
| MG211584 | Xpo1 (tGFP-tagged) - Mouse exportin 1, CRM1 homolog (yeast) (Xpo1), transcript variant 2 |
CNY 11,690.00 |
|
| MR211584L3 | Lenti ORF clone of Xpo1 (Myc-DDK-tagged) - Mouse exportin 1, CRM1 homolog (yeast) (Xpo1), transcript variant 2 |
CNY 12,540.00 |
|
| MR211584L4 | Lenti ORF clone of Xpo1 (mGFP-tagged) - Mouse exportin 1, CRM1 homolog (yeast) (Xpo1), transcript variant 2 |
CNY 12,540.00 |
