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Grip1 (BC067398) Mouse Tagged ORF Clone
CAT＃: MR211562
- TrueORF®
Grip1 (Myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:76573 IMAGE:30096759)
ORF Plasmid: tGFP
"BC067398" in other vectors (4)
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CNY 10,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4931400F03Rik; eb; GRIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211562 representing BC067398
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGCTGTCTCTTTTAAATGCCGCTGTCAAATTCTAAGGCGACTTACCAAAGATGAGAGTCCCTACA CTAAATCTGCCAGCCAGACAAAGCCGCCCGATGGAGCATTGGCTGTGAGGAGACAGAGCATCCCAGAGGA ATTCAAGGGCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCGGGA GGCATTGATAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTGACC AGCTGGATGTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGAGAT CATCAGCCTGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTCTCT GTACAAGGATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACGCCTTTGGTT TTGTCATCCGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCGTCC TGGAGGGCCTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATTCGG CTCCTGGGAACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGCTGA TAGAATACGATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGCCAA AACTCCAGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTCATA GACAAAATCAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCTCCA TTGACGGCACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGACCA GGTCAAGCTGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGCGGCTATG GTGCCCTCATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTGAACATGGGGACTTTACCTC GAAGCCTCTACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGAAAAAGAAAGACTTCAAAAG CTCACTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCACACTGAAACCACAGAGGTT GTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGCGTGTTTGCCACAGAGACGC TCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGAGATGTGGTGTGCTACAGAT TGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTTCGAGGAAGCCAATCAACTC CTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGATGTTGCAGAGTCTGTCATCC CAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAACTTGGAATAACCATCAGTTC GCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAAAGGCAGTGTGGCACACAGA ACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGGCTGGATAACTGTTCCATGG AAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAATCCGCAAAGATGAAGATAA CTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAAGCGCTATGGGGGGCCCCTT GGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCGAGCCTCACTAAAGGGGGAT TAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCAATAGCAGCAGCTTGAAGGG GAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGTCACCCTGAAAATTAAGAAA CAGACAGATGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGCCACTCGGGGGACCTAGGAG ATGGTGAGGAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATGCGTACCCCTCCACGGTGCC CAGCGTGGACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAGCTATGGGAGTCAAGGCTCA ACTTTTCAGACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCAAAGCAAAGAACCAGCCTGT CCCCAGTCCCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTAATGAAGACTGGGATCGATC CACAGCCAGTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGAGGAAAACTTCTGGTCTCAA GCATTGGAGGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTTGAGGCAACAATCATGTCGG GGAGTACTATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGGGGCGACAGGCCAGCTTCCA GGAACGGAGCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACACCCTGCCCTCAGACGTGGGC AGAAAGTCTGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATGTCCCCAACTCCGGTGGAGC TACACAAGGTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCAGTGTGGCAGATGGCCTGCT GGAGAAAGGCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGTTGGGGGCTTGAAGCCCTAC GACAGGCTCTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGCCTGGTGGTGCCTCTCATAG CTGAATCTGGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCTCCCAGAAGTCGATAGAACA GCCGGCTCTGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCAACCCAGCCACGGTGGTAAT CTAGAGACACGAGAACCCACTAACACACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211562 representing BC067398
Red=Cloning site Green=Tags(s) MIAVSFKCRCQILRRLTKDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGTTLGLTVSG GIDKDGKPRVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPVS VQGSSVMFRTVEVTLHKEGNAFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGIR LLGTTHAEAMSILKQCGQEATLLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIVI DKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHAAM VPSSSPTSMSAYSLSSLNMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEV VLTADPVTGFGIQLQGSVFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQL LRDSSITSKVTLEIEFDVAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHR TGTLELGDKLLAIDNIRLDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPL GITISGTEEPFDPIIISSLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKK QTDAQSASSPKKFPIPGHSGDLGDGEEDPSPIQKPGKLSDAYPSTVPSVDSAVDSWDGSGIDASYGSQGS TFQTSGYNYNTYDWRSPKQRTSLSPVPKPRSQTYPDVGLSNEDWDRSTASGFVGASDSADAEQEENFWSQ ALEDLETCGQSGILRELEATIMSGSTMSLNHEAPMARSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVG RKSVTLRKMKQEIKEIMSPTPVELHKVTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDVGGLKPY DRLLQVNHVRTRDFDCCLVVPLIAESGNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGGN LETREPTNTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC067398 |
| ORF Size | 3180 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC067398.1 |
| RefSeq Size | 5024 bp |
| RefSeq ORF | 3182 bp |
| Locus ID | 74053 |
| MW | 184.1 kDa |
| Gene Summary | This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223103 | Grip1 (untagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:76573 IMAGE:30096759), (10ug) |
CNY 12,920.00 |
|
| MG211562 | Grip1 (tGFP-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:76573 IMAGE:30096759) |
CNY 11,590.00 |
|
| MR211562L3 | Lenti ORF clone of Grip1 (Myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:76573 IMAGE:30096759) |
CNY 12,450.00 |
|
| MR211562L4 | Lenti ORF clone of Grip1 (mGFP-tagged) - Mouse glutamate receptor interacting protein 1 (cDNA clone MGC:76573 IMAGE:30096759) |
CNY 12,450.00 |
