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Timeless (BC058641) Mouse Tagged ORF Clone
CAT＃: MR211559
- TrueORF®
Timeless (Myc-DDK-tagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105)
ORF Plasmid: tGFP
"BC058641" in other vectors (4)
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CNY 7,368.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | tim, Debt69 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211559 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGTACATGATGAACTGTGAACTTCTAGCCACGTGTAGCGCCCTTGGGTACTTGGAAGGAGGGA CTTACCACAAGGAGCCGGATTGCCTGGAGAGTGTGAAGGATTTGATCCGATACCTGAGGCACGAGGATGA GACCCGAGATGTGCGGCAGCAGCTGGGAGCTGCACAGATCCTGCAGAGCGACCTCCTGCCAATCCTCACG CAGCATCGCCAGGACAAGCCTCTCTTCGATGCCGTGATCAGGCTGATGGTAAATTTGACACAGCCAGCCT TGCTCTGTTTTGGCAGCGTGCCTAAGGACTCCAGTGTACGGCACCATTTTCTGCAGGTTCTAACGTACCT GCAAGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCATTTGGAGTCCTCAGCGAGACCTTGTATGAATTG CTACAGCTGGGCTGGGAGGATCGGCAAGAAGAAGACAACTTGCTGATCGAGCGGATCCTTCTGCTGGTCA GAAATATTCTCCATGTCCCGGCCAACCTTGAGCAGGAGAAGAGTATCGATGATGATGCCAGCATCCACGA CCGTCTCCTTTGGGCAATTCACCTCAGTGGCATGGACGACTTGCTCCTCTTCCTGTCCAGCTCATCCGCC GAGCAGCAGTGGAGCCTCCATGTGCTGGAGATCATCTCCCTCATGTTCCGAGACCAGACCCCTGAGCAGC TAGCGGGAGTAGGGCAGGGACGCTTGGCTCAGGAGCGAAGCACGGATGTGGCAGAATTGGAGGTGCTGCG CCAACGGGAGATGGCGGAGAAGAGAGCTCGGGCCCTCCAGCGAGGAAACAGGCACTCTCGATTTGGGGGC TCCTACATTGTCCAGGGGTTGAAATCTATTGGGGAGAAGGATGTCGTCTTTCACAAAGGCCTTCACAATC TCCAGAACTACAGCTCAGATCTGGGAAAGCAGCCCAGGAGGGTGCCCAAGCGTCGTCAGGCTGCCCAGGA GCTGTCTGTCCATCGCCGCTCTGTCCTGAATGTGAGACTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG GAGAACTGCTACAACCCGCTCATGGGCGCGGTCAAGGATCATCTGCTTCGGGAGAGAGCGCAGCAGCATG ACGAGACTTACTACATGTGGGCAATGGCTTTCTTCATGGCCTTCAACCGAGCTGCCACCTTCCGCCCCGG CCTTGTTTCTGAGACCCTCAGTATCCGTACCTTTCACTTTGTGGAGCAGAACCTCACCAACTACTACGAG ATGATGCTGACAGACCGCAAGGAGGCCGCCTCCTGGGCGCGCAGGATGCACCTGGCCCTGAAGGCCTACC AGGAGCTGCTGGCCACGGTGAACGAGATGGACATGTGCCCAGATGAGGCTGTTAGGGAGAGCAGTCGTAT CATCAAAAACAACATTTTCTATATGATGGAGTACCGAGAACTATTCCTGGCGCTCTTTCGAAAGTTTGAT GAGAGATACCATCCACGCTCATTCCTTCGAGACCTGGTGGAAACCACCCACCTCTTCCTCAAAATGTTGG AGCGCTTTTGCCGGAGCCGCGGGAACCTGATGGTGCAGAACAAAAGAAAAAAGAGGAAAAAGAAAAAGAA GGTTCAGGACCAGGGTGTTGCTTTCTCACAAAGCCCCGGGGAGCTGGAGGCCATGTGGCCAGCCCTGGCA GAGCAGCTGCTGCAGTGTGCCCAGGACCCTGAGCTCAGTGTGGACCCCGTCGTTCCCTTTGATGCGGCCT CAGAGGTGCCAGTGGAGGAGCAGCGGGTAGAAGCCATGGTGAGGATCCAAGACTGCCTTACGGCTGGCCA GGCCCCGCAAGCCCTGGCCCTCCTGCGGTCTGCCCGGGAAGTGTGGCCTGAAGGAAATGCGTTTGGCTCT CCAGTCATTTCCCCAGGGGAAGAAATGCAGTTGCTGAAACAAATCCTCTCCACGCCCCTTCCCCGGCAGC AGGAGCCAGAAGAAGGAGATGCAGAGGAGGAAGAGGAAGAGGAGGAGGAAGAGGAGTTACAGGTGGTCCA GGTGTCAGAGAAGGAGTTTAACTTTCTGGAATACCTGAAACGCTTCGCATCCTCAACCATCGTGCGGGCC TACGTGCTTCTCCTGCGGAGCTACAGGCAGAACAGTGCTCACACCAACCACTGCATCGCCAAGATGCTGC ACCGGCTGGCCCATGGCCTGGGGATGGAAGCCCTGCTTTTCCAGCTGTCCCTGTTCTGCCTCTTCAATCG GCTGCTTAGTGACCCAGCTGCTGCGGCCTACAAAGAGCTAGTGACTTTTGCCAAATACATCATTGGCAAG TTCTTTGCGTTGGCTGCCGTGAACCAGAAAGCGTTTGTGGAGCTGCTGTTCTGGAAGAACACCGCAGTGG TTCGGGAAATGACCCAGGGATATGGCTCCCTCGACAGTGGGTCTTCCAGCCACAGAGCTCCTCTGTGGAG CCCTGAGGAAGAGGCCCAGCTTCAGGAACTATACCTCGCCCACAAGGATGTGGAAGGTCAAGATGTAGTG GAAACCATATTGGCGCACCTGAAAGTCGTTCCTCGAACACGCAAGCAGGTCATCCACCACCTGGTCCGGA TGGGCCTGGCCGACAGCGTCAAGGAGTTCCAGAAGAGGAAAGGGACCCAGATTGTCTTGTGGACGGAGGA CCAGGAGCTGGAGTTACAGCGGCTCTTTGAGGAGTTCCGGGACTCTGATGATGTTCTTGGTCAAATCATG AAGAATATCACAGCCAAACGTTCACGGGCTCGAGTAGTGGACAAACTGTTGGCCCTGGGGTTGGTGTCTG AGCGGAGGCAGCTATACAAGAAACGGAGAAAGAAGCTGGCGCCTTCTTGCATGAATGGAGAAAAGTCCCC GAGAGACCCCTGGCAGGAAGATCCGGAAGAGGAGCCAGAGCCAGGAGTCCCCGGAGAGCAGGGTCCCAGT GAGGAGCACCGGACAGAAGCCCTGAGAGCCCTTCTGTCAGCCCGTAAGAGGAAAGCAGGCCTGGGGCCTA CAGAAGAGGAGGCCACTGGGGAGGAAGAATGGAACTCAGCGCCCAAGAAGCGGCAACTGCTGGACAGCGA CGAAGAGGAAGATGATGAGGGGAGGAGGCAAGCAGTGTCGGGAACGCCAAGAGTCCACAGGAAGAAACGG TTTCAGATTGAGGATGAGGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211559 protein sequence
Red=Cloning site Green=Tags(s) MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILT QHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYEL LQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSA EQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGG SYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFL ENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYE MMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFD ERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALA EQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGS PVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRA YVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGK FFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVV ETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIM KNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMNGEKSPRDPWQEDPEEEPEPGVPGEQGPS EEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKR FQIEDEDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC058641 |
| ORF Size | 3174 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058641, AAH58641 |
| RefSeq Size | 4002 bp |
| RefSeq ORF | 3176 bp |
| Locus ID | 21853 |
| MW | 122 kDa |
| Gene Summary | The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223076 | Timeless (untagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105), (10ug) |
CNY 12,920.00 |
|
| MG211559 | Timeless (tGFP-tagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105) |
CNY 6,560.00 |
|
| MR211559L3 | Lenti ORF clone of Timeless (Myc-DDK-tagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105) |
CNY 7,890.00 |
|
| MR211559L4 | Lenti ORF clone of Timeless (mGFP-tagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105) |
CNY 7,890.00 |
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