Timeless (BC058641) Mouse Tagged ORF Clone

CAT#: MR211559

  • TrueORF®

Timeless (Myc-DDK-tagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105)

ORF Plasmid: DDK tGFP



  "BC058641" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,368.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms tim, Debt69
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211559 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTTGTACATGATGAACTGTGAACTTCTAGCCACGTGTAGCGCCCTTGGGTACTTGGAAGGAGGGA
CTTACCACAAGGAGCCGGATTGCCTGGAGAGTGTGAAGGATTTGATCCGATACCTGAGGCACGAGGATGA
GACCCGAGATGTGCGGCAGCAGCTGGGAGCTGCACAGATCCTGCAGAGCGACCTCCTGCCAATCCTCACG
CAGCATCGCCAGGACAAGCCTCTCTTCGATGCCGTGATCAGGCTGATGGTAAATTTGACACAGCCAGCCT
TGCTCTGTTTTGGCAGCGTGCCTAAGGACTCCAGTGTACGGCACCATTTTCTGCAGGTTCTAACGTACCT
GCAAGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCATTTGGAGTCCTCAGCGAGACCTTGTATGAATTG
CTACAGCTGGGCTGGGAGGATCGGCAAGAAGAAGACAACTTGCTGATCGAGCGGATCCTTCTGCTGGTCA
GAAATATTCTCCATGTCCCGGCCAACCTTGAGCAGGAGAAGAGTATCGATGATGATGCCAGCATCCACGA
CCGTCTCCTTTGGGCAATTCACCTCAGTGGCATGGACGACTTGCTCCTCTTCCTGTCCAGCTCATCCGCC
GAGCAGCAGTGGAGCCTCCATGTGCTGGAGATCATCTCCCTCATGTTCCGAGACCAGACCCCTGAGCAGC
TAGCGGGAGTAGGGCAGGGACGCTTGGCTCAGGAGCGAAGCACGGATGTGGCAGAATTGGAGGTGCTGCG
CCAACGGGAGATGGCGGAGAAGAGAGCTCGGGCCCTCCAGCGAGGAAACAGGCACTCTCGATTTGGGGGC
TCCTACATTGTCCAGGGGTTGAAATCTATTGGGGAGAAGGATGTCGTCTTTCACAAAGGCCTTCACAATC
TCCAGAACTACAGCTCAGATCTGGGAAAGCAGCCCAGGAGGGTGCCCAAGCGTCGTCAGGCTGCCCAGGA
GCTGTCTGTCCATCGCCGCTCTGTCCTGAATGTGAGACTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG
GAGAACTGCTACAACCCGCTCATGGGCGCGGTCAAGGATCATCTGCTTCGGGAGAGAGCGCAGCAGCATG
ACGAGACTTACTACATGTGGGCAATGGCTTTCTTCATGGCCTTCAACCGAGCTGCCACCTTCCGCCCCGG
CCTTGTTTCTGAGACCCTCAGTATCCGTACCTTTCACTTTGTGGAGCAGAACCTCACCAACTACTACGAG
ATGATGCTGACAGACCGCAAGGAGGCCGCCTCCTGGGCGCGCAGGATGCACCTGGCCCTGAAGGCCTACC
AGGAGCTGCTGGCCACGGTGAACGAGATGGACATGTGCCCAGATGAGGCTGTTAGGGAGAGCAGTCGTAT
CATCAAAAACAACATTTTCTATATGATGGAGTACCGAGAACTATTCCTGGCGCTCTTTCGAAAGTTTGAT
GAGAGATACCATCCACGCTCATTCCTTCGAGACCTGGTGGAAACCACCCACCTCTTCCTCAAAATGTTGG
AGCGCTTTTGCCGGAGCCGCGGGAACCTGATGGTGCAGAACAAAAGAAAAAAGAGGAAAAAGAAAAAGAA
GGTTCAGGACCAGGGTGTTGCTTTCTCACAAAGCCCCGGGGAGCTGGAGGCCATGTGGCCAGCCCTGGCA
GAGCAGCTGCTGCAGTGTGCCCAGGACCCTGAGCTCAGTGTGGACCCCGTCGTTCCCTTTGATGCGGCCT
CAGAGGTGCCAGTGGAGGAGCAGCGGGTAGAAGCCATGGTGAGGATCCAAGACTGCCTTACGGCTGGCCA
GGCCCCGCAAGCCCTGGCCCTCCTGCGGTCTGCCCGGGAAGTGTGGCCTGAAGGAAATGCGTTTGGCTCT
CCAGTCATTTCCCCAGGGGAAGAAATGCAGTTGCTGAAACAAATCCTCTCCACGCCCCTTCCCCGGCAGC
AGGAGCCAGAAGAAGGAGATGCAGAGGAGGAAGAGGAAGAGGAGGAGGAAGAGGAGTTACAGGTGGTCCA
GGTGTCAGAGAAGGAGTTTAACTTTCTGGAATACCTGAAACGCTTCGCATCCTCAACCATCGTGCGGGCC
TACGTGCTTCTCCTGCGGAGCTACAGGCAGAACAGTGCTCACACCAACCACTGCATCGCCAAGATGCTGC
ACCGGCTGGCCCATGGCCTGGGGATGGAAGCCCTGCTTTTCCAGCTGTCCCTGTTCTGCCTCTTCAATCG
GCTGCTTAGTGACCCAGCTGCTGCGGCCTACAAAGAGCTAGTGACTTTTGCCAAATACATCATTGGCAAG
TTCTTTGCGTTGGCTGCCGTGAACCAGAAAGCGTTTGTGGAGCTGCTGTTCTGGAAGAACACCGCAGTGG
TTCGGGAAATGACCCAGGGATATGGCTCCCTCGACAGTGGGTCTTCCAGCCACAGAGCTCCTCTGTGGAG
CCCTGAGGAAGAGGCCCAGCTTCAGGAACTATACCTCGCCCACAAGGATGTGGAAGGTCAAGATGTAGTG
GAAACCATATTGGCGCACCTGAAAGTCGTTCCTCGAACACGCAAGCAGGTCATCCACCACCTGGTCCGGA
TGGGCCTGGCCGACAGCGTCAAGGAGTTCCAGAAGAGGAAAGGGACCCAGATTGTCTTGTGGACGGAGGA
CCAGGAGCTGGAGTTACAGCGGCTCTTTGAGGAGTTCCGGGACTCTGATGATGTTCTTGGTCAAATCATG
AAGAATATCACAGCCAAACGTTCACGGGCTCGAGTAGTGGACAAACTGTTGGCCCTGGGGTTGGTGTCTG
AGCGGAGGCAGCTATACAAGAAACGGAGAAAGAAGCTGGCGCCTTCTTGCATGAATGGAGAAAAGTCCCC
GAGAGACCCCTGGCAGGAAGATCCGGAAGAGGAGCCAGAGCCAGGAGTCCCCGGAGAGCAGGGTCCCAGT
GAGGAGCACCGGACAGAAGCCCTGAGAGCCCTTCTGTCAGCCCGTAAGAGGAAAGCAGGCCTGGGGCCTA
CAGAAGAGGAGGCCACTGGGGAGGAAGAATGGAACTCAGCGCCCAAGAAGCGGCAACTGCTGGACAGCGA
CGAAGAGGAAGATGATGAGGGGAGGAGGCAAGCAGTGTCGGGAACGCCAAGAGTCCACAGGAAGAAACGG
TTTCAGATTGAGGATGAGGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211559 protein sequence
Red=Cloning site Green=Tags(s)

MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILT
QHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYEL
LQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSA
EQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGG
SYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFL
ENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYE
MMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFD
ERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALA
EQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGS
PVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRA
YVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGK
FFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVV
ETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIM
KNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMNGEKSPRDPWQEDPEEEPEPGVPGEQGPS
EEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKR
FQIEDEDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC058641
ORF Size 3174 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058641, AAH58641
RefSeq Size 4002 bp
RefSeq ORF 3176 bp
Locus ID 21853
MW 122 kDa
Gene Summary The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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