Uba1 (NM_001136085) Mouse Tagged Lenti ORF Clone
CAT#: MR211556L4
- LentiORF®
Lenti ORF clone of Uba1 (mGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 2
"NM_001136085" in other vectors (3)
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CNY 10,260.00
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | A1; A1S9; Sb; Sbx; Ube-1; Ube1x |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR211556).
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Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001136085 |
ORF Size | 3177 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001136085.1, NP_001129557.1 |
RefSeq Size | 4073 bp |
RefSeq ORF | 3177 bp |
Locus ID | 22201 |
UniProt ID | Q02053 |
Gene Summary | This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223384 | Uba1 (untagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 2, (10ug) |
CNY 13,590.00 |
|
MR211556 | Uba1 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 2 |
CNY 7,376.00 |
|
MR211556L3 | Lenti ORF clone of Uba1 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 2 |
CNY 10,260.00 |