United States
Germany
Japan
United Kingdom
China
Usp25 (NM_013918) Mouse Tagged ORF Clone
CAT＃: MR211552
- TrueORF®
Usp25 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 25 (Usp25)
ORF Plasmid: tGFP
"NM_013918" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,344.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211552 representing NM_013918
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGTGGAGCAGAATGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACATTTTTGAATCAGC TGAGAGAAATTACTGGGATTAATGACGCCCAGATACTCCAACAAGCCCTGAAGGATAGTAACGGAAACTT GGAATTAGCAGTAGCCTTCCTCACTGCAAAGAATGCCAAGACCCCCCCACAGGAGGAGACAGGCTATTAC CAGACAGCGCTTCCAGGCAACGACAGGTACATCAGCGTGGGAAGCCAGGCAGATGCAAATGTAATTGATC TCACTGGAGATGACAAAGATGATCTTCAGAGAGCGATTGCCTTGAGTTTGGCGGAGTCAAACAGGGCATT CAGGGAGACGGGCATAACCGATGAAGAGCAGGCCATCAGTAGAGTTCTTGAAGCCAGCATAGCAGAAAAT AAAGCATGCTTGAAGAGAACACCTATAGAAGTTTGGAGGGATTCTCGAAACCCCTATGACAGAAAAAGAC AAGAGAAAGCTCCAGTCGGTCTGAAGAATGTCGGCAACACCTGCTGGTTTAGTGCAGTTATTCAGTCATT ATTCAATCTTTTGGAGTTTAGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAAGATTTACCC CGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGGGAACTGAGGTATCTGTTTGCACTTCTTGTTG GTACCAAGAGGAAGTATGTTGATCCGTCCAGAGCAGTTGAGATTCTGAAGGATGCCTTCAAATCCAATGA CTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAGTTATTGGACTGGTTGGAAGATGCCTTCCAAATG AAAGCTGAAGAGGAAACGGATGAAGAGAAGCCAAAGAACCCTATGGTAGAGCTGTTCTATGGAAGATTCC TAGCCATGGGAGTGCTTGAAGGAAAAAAATTTGAGAACACTGAAATGTTCGGTCAGTATCCACTTCAGGT GAACGGGTTCAAAGACCTGCATGAGTGCCTGGAGGCCGCCATGATCGAAGGGGAGATCGAATCCTTACAC TCCGATAACTCAGGAAAGTCAGGCCAGGAGCATTGGTTCACTGAATTGCCGCCGGTATTAACATTTGAAC TGTCAAGATTTGAATTTAACCAGGCATTGGGAAGACCAGAAAAAATTCACAATAAACTAGAATTTCCCCA AGTTTTATATCTGGACAGGTATATGCACAGAAATAGAGAAATAACAAGAATTAAACGTGAAGAGATCAAG AGACTTAAAGATTACCTCACAGTATTACAACAAAGATTAGAGAGATATTTAAGCTATGGTTCCGGTCCCA AAAGATTCCCATTGGTAGATGTTCTACAGTATGCATTGGAGTTTGCCTCCAGTAAACCTGTGTGCACTTC CCCCGTTGATGACATCGATGCTAGTTCCTCAGCAAGTGGCCCCCTACCATCACAGTCCTTACCAAGCACA ACAGAACAGCAGGGACCCTGTGCTTCAGATCTGCCCGGCTCCTCCTCACCCGCATCAGGTGCCGCCCTGC CCTTGAGGTCAGTGATTCACAAACCCTTCACTCAGTCTCGGATACCTCCAGATTTGCCCATGCATCCGGC ACCAAGGCACATCACAGAGGAAGAACTGTGCGTGCTGGAGAGCTGTTTACATCGCTGGAGGACGGAAATA GAAAATGACACGCGAGATCTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAACTCATGTACTCGG ACAAATCCATGATCCAAGTCCCTTACCGCCTCCATGCCGTCCTAGTTCATGAGGGCCAGGCTAATGCCGG CCACTACTGGGCGTACATCTTTGACCACCGGGAGAGCCGGTGGATGAAGTATAATGATATTGCTGTGACC AAGTCTTCATGGGAAGAGCTAGTGAGGGACTCTTTCGGTGGTTATCGAAATGCCAGTGCATACTGCTTAA TGTACATAGACGACAAGGCACAGTTCCTAATACAAGAGGAGTTTAATAAAGAGACTGGGCAGGCCCTTGT TGGAATGGAAACCTTACCTCCAGACTTGAGAGACTTTGTTGAGGAGGACAACCAGCGCTTTGAGAAGGAA CTGGAAGAATGGGACACGCAGCTTGCCCAACGATCACTGCAAGAAAAGCTGCTAGCTGCGCCGAAACTGC GGGAAGCAGAGGCTTCGGCCACCACAGCACAAGCAGGAGGCGCAGACTATCTAGAGCAGCCATCAAGAAG TGACTTGTCAAAGCACTGGAAAGAAGAAACGCTCCGAGTGATTGCCAAGGCGTCACATGATCTTGAAGAT AAAGGGCCAGAGACAGTTTTGCAGTCGGCAATTAAATTAGAGTATTCTAGGCTGGTTAAGTTGGCCCAAG AAGATACTCCACCAGAAACAGATTATCGTTTACATCATGTCCTGGTCTACTTTATCCAGAACCAGGCACC AAAGAAAATCATTGAGAAAACATTACTCGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGT CACAACATAATGAAAGTTGCCCAAGCCAAACTGGAAATGATCAAACCTGAAGAAGTGAACTTGGAAGAAT ATGAGGAGTGGCATGCAGATTATAAGAAATTCCGAGAAACAACTATGTATCTCATAACTGGACTAGAAAA CTTTCAGAGAGAAAGTTATATAGACTCCTTGCTGTTCCTTCTCTGTGCTTATCAGAATAACAAAGAGCTC TTGTCTAAGGGTCCGTACAGAGGCCACGATGGGGAGCTGATATCACATTACAGAAGAGAGTGCTTACTGA AATTAAACGAGCAAGCGGCCGAGCTGTTTGAGTCTGGAGAGGACGGAGACGTGAACAATGGCTTAATTAT TATGAATGAGTTTATCGTTCCCTTTCTGCCCTTGTTGCTGGTGGATGACATGGAAGAGAAAGATATCCTT GCTGTGGAAGACATGAGGAATCGCTGGTGTTCATACCTGGGGCAAGAAATGGAAGCAAACCTCCAAGAAA AGCTCACGGATTTTCTGCCAAAACTGCTTGATTGTTCTACAGAGATTAAAGGTTTCCACGAGCCACCAAA GTTACCTTCCTACTCTGCGCACGAACTCTGTGAGCGATTCGCCCGGATCATGTTGTCCCTCAGTCGTACG CCTGCTGATGGAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211552 representing NM_013918
Red=Cloning site Green=Tags(s) MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPPQEETGYY QTALPGNDRYISVGSQADANVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAEN KACLKRTPIEVWRDSRNPYDRKRQEKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLP RNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQM KAEEETDEEKPKNPMVELFYGRFLAMGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLH SDNSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIK RLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSSASGPLPSQSLPST TEQQGPCASDLPGSSSPASGAALPLRSVIHKPFTQSRIPPDLPMHPAPRHITEEELCVLESCLHRWRTEI ENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVT KSSWEELVRDSFGGYRNASAYCLMYIDDKAQFLIQEEFNKETGQALVGMETLPPDLRDFVEEDNQRFEKE LEEWDTQLAQRSLQEKLLAAPKLREAEASATTAQAGGADYLEQPSRSDLSKHWKEETLRVIAKASHDLED KGPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVLVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERC HNIMKVAQAKLEMIKPEEVNLEEYEEWHADYKKFRETTMYLITGLENFQRESYIDSLLFLLCAYQNNKEL LSKGPYRGHDGELISHYRRECLLKLNEQAAELFESGEDGDVNNGLIIMNEFIVPFLPLLLVDDMEEKDIL AVEDMRNRWCSYLGQEMEANLQEKLTDFLPKLLDCSTEIKGFHEPPKLPSYSAHELCERFARIMLSLSRT PADGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013918 |
| ORF Size | 3165 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013918.2, NP_038946.2 |
| RefSeq Size | 4432 bp |
| RefSeq ORF | 3168 bp |
| Locus ID | 30940 |
| UniProt ID | P57080 |
| MW | 121.9 kDa |
| Gene Summary | Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223385 | Usp25 (untagged) - Mouse ubiquitin specific peptidase 25 (Usp25), (10ug) |
CNY 13,590.00 |
|
| MG211552 | Usp25 (tGFP-tagged) - Mouse ubiquitin specific peptidase 25 (Usp25) |
CNY 9,220.00 |
|
| MR211552L3 | Lenti ORF clone of Usp25 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 25 (Usp25) |
CNY 10,260.00 |
|
| MR211552L4 | Lenti ORF clone of Usp25 (mGFP-tagged) - Mouse ubiquitin specific peptidase 25 (Usp25) |
CNY 10,260.00 |
