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Mical1 (NM_138315) Mouse Tagged ORF Clone
CAT＃: MR211527
- TrueORF®
Mical1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 1
ORF Plasmid: tGFP
"NM_138315" in other vectors (4)
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CNY 10,952.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MICAL; MICAL-1; Nical |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCACCCGCCTCCACCAACCCAGCACATGACCACTTTGAGACCTTTGTGCAGGCACAGCTGTGCC AGGATGTACTGAGTAGCTTTCAGGGGCTCTGTAGAGCCCTGGGAGTGGAGTCTGGTGGGGGATTGTCCCA GTACCACAAGATCAAGGCCCAGCTCAACTACTGGAGTGCCAAGTCACTGTGGGCCAAGTTGGACAAGAGA GCGAGCCAGCCTGTGTACCAGCAAGGCCAGGCCTGTACCAACACCAAGTGTCTCGTGGTAGGTGCCGGGC CTTGCGGACTTCGGGCTGCTGTGGAGTTGGCACTGTTAGGTGCCCGAGTGGTACTTGTGGAAAAGCGTAT CAAGTTCTCTAGGCACAATGTGCTCCATCTCTGGCCCTTCACCATCCATGACCTTCGGGCACTTGGGGCC AAGAAGTTCTACGGGCGCTTCTGTACTGGCACCCTGGACCATATCAGCATCCGACAGCTTCAGCTGCTTC TACTGAAGGTGGCCTTACTGTTGGGGGTGGAGATTCACTGGGGCGTCAAATTCACTGGCCTCCAGCCCCC TCCCAGAAAAGGGAGTGGCTGGCGTGCTCAGCTCCAGCCCAACCCCCCAGCCCAACTGGCCAGCTATGAG TTTGATGTCCTCATCTCAGCTGCAGGAGGCAAATTTGTCCCTGAAGGCTTCACTATACGAGAGATGCGTG GCAAACTGGCCATTGGCATCACAGCCAACTTTGTGAATGGGCGCACGGTGGAGGAGACACAGGTGCCGGA GATCAGTGGTGTAGCTCGAATCTACAACCAAAAATTCTTCCAGAGCCTGCTCAAAGCCACAGGTATTGAT CTGGAGAACATTGTATACTACAAAGACGAGACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGC TGAGGCTGGGGGTGCTGCGCCAGGACTTGTCAGAGACTGATCAGCTGCTGGGCAAAGCTAATGTGGTACC GGAGGCTCTGCAGCGCTTTGCCAGAGCAGCGGCTGACTTCGCCACACATGGCAAGCTTGGGAAACTGGAG TTTGCTCAGGATGCACGCGGGCGGCCTGATGTGGCGGCCTTCGACTTCACAAGCATGATGCGGGCAGAGA GTTCCGCTCGTGTCCAAGAAAAGCATGGTGCTCGCCTACTGCTGGGGCTGGTGGGGGACTGCCTAGTGGA GCCCTTCTGGCCTCTGGGCACTGGAGTAGCCCGAGGCTTCTTGGCAGCCTTCGATGCAGCCTGGATGGTG AAGCGGTGGGCAGAGGGCGCTGGGCCGCTAGAGGTGTTGGCTGAACGCGAGAGCTTGTACCAGCTTCTGT CACAAACATCCCCCGAGAATATGCATCGAAATGTAGCCCAGTATGGGTTGGACCCTGCCACCCGATACCC CAACCTGAACCTCCGGGCTGTAACCCCCAATCAGGTACAGGACCTCTATGATATGATGGACAAGGAGCAT GCTCAGAGGAAGAGTGACGAGCCGGATTCCAGGAAGACAACCACAGGGTCCGCAGGCACGGAGGAGCTTC TGCACTGGTGCCAGGAGCAGACAGCTGGCTTTCCTGGAGTCCATGTCACTGACTTTTCTTCCTCGTGGGC TGATGGGCTAGCTCTGTGTGCCCTGGTACACCACCTACAGCCTGGCCTGCTGGAACCCTCGGAGCTGCAG GGCATGGGAGCTCTAGAAGCCACTACCTGGGCACTGAGGGTGGCAGAACATGAGCTAGGCATCACACCAG TGTTGTCTGCACAGGCAGTCATGGCTGGCAGTGACCCACTGGGTCTCATTGCCTACCTCAGCCACTTCCA CAGTGCCTTCAAGAACACGAGCCACAGCTCAGGCCTTGTCAGCCAGCCCTCTGGAACCCCCAGTGCTATA CTTTTCCTTGGCAAACTCCAGAGGAGCCTACAACGGACCCGCGCCAAGGTAGACGAGGAGACTCCGAGCA CTGAGGAGCCGCCTGTCTCCGAGCCCAGTATGTCTCCAAACACTCCAGAGCTCTCTGAACACCAGGAGGC TGGGGCAGAGGAGCTGTGTGAACTCTGTGGGAAACATCTGTACATCCTAGAACGCTTCTGTGTGGATGGC CATTTCTTTCACCGGAGCTGCTTCTGCTGCCATACCTGTGAGGCCACATTGTGGCCAGGTGGCTATGGGC AACATCCAGGAGATGGACATTTCTACTGTCTCCAGCACCTACCCCAGGAGGACCAAAAGGAGGCTGACAA CAATGGAAGTCTGGAGAGCCAGGAGCTCCCAACACCAGGAGACAGCAACATGCAGCCAGACCCCTCCTCT CCTCCTGTGACGAGGGTCAGCCCTGTCCCAAGCCCCAGCCAGCCTGCACGTCGGCTGATCCGGCTCTCCA GTTTAGAACGCCTACGGCTGTCCTCCCTGAATATCATCCCTGACTCAGGAGCGGAGCCTCCTCCCAAACC CCCAAGGAGCTGCTCAGACTTGGCCCGAGAATCCCTGAAGAGCAGCTTTGTGGGCTGGGGTGTGCCAGTC CAAGCACCACAAGTTCCTGAAGCCATAGAGAAAGGGGATGATGAGGAGGAGGAAGAGGAAGAAGAGGAGG AGGAGGAGGAGCCACTGCCACCTTTGGAGCCAGAGTTGGAGCAGACTCTGCTGACCTTGGCCAAGAACCC AGGTGCCATGACTAAGTACCCAACGTGGCGTCGAACCCTCATGCGCCGTGCCAAAGAGGAGGAGATGAAG AGGTTTTGCAAGGCCCAGGCCATCCAGCGAAGACTGAACGAGATTGAGGCCACTATGCGGGAGCTGGAGG CCGAAGGCACGAAGCTGGAGCTGGCCTTGAGGAAAGAGAGTAGCTCTCCAGAACAGCAGAAGAAACTCTG GCTGGATCAGCTGCTACGGCTCATTCAGAAGAAGAACAGCCTAGTGACTGAAGAGGCCGAGCTCATGATC ACGGTGCAGGAGCTGGATCTGGAAGAGAAGCAGCGGCAGCTAGACCACGAGTTGCGGGGCTACATGAATC GGGAAGAAACTATGAAGACAGAGGCCGACCTGCAGTCTGAGAACCAGGTCCTAAGGAAGCTGTTGGAGGT GGTGAACCAGCGGGATGCTCTGATCCAATTCCAGGAGGAACGGAGGCTCAGAGAGATGCCTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211527 protein sequence
Red=Cloning site Green=Tags(s) MASPASTNPAHDHFETFVQAQLCQDVLSSFQGLCRALGVESGGGLSQYHKIKAQLNYWSAKSLWAKLDKR ASQPVYQQGQACTNTKCLVVGAGPCGLRAAVELALLGARVVLVEKRIKFSRHNVLHLWPFTIHDLRALGA KKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVKFTGLQPPPRKGSGWRAQLQPNPPAQLASYE FDVLISAAGGKFVPEGFTIREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQKFFQSLLKATGID LENIVYYKDETHYFVMTAKKQCLLRLGVLRQDLSETDQLLGKANVVPEALQRFARAAADFATHGKLGKLE FAQDARGRPDVAAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMV KRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVQDLYDMMDKEH AQRKSDEPDSRKTTTGSAGTEELLHWCQEQTAGFPGVHVTDFSSSWADGLALCALVHHLQPGLLEPSELQ GMGALEATTWALRVAEHELGITPVLSAQAVMAGSDPLGLIAYLSHFHSAFKNTSHSSGLVSQPSGTPSAI LFLGKLQRSLQRTRAKVDEETPSTEEPPVSEPSMSPNTPELSEHQEAGAEELCELCGKHLYILERFCVDG HFFHRSCFCCHTCEATLWPGGYGQHPGDGHFYCLQHLPQEDQKEADNNGSLESQELPTPGDSNMQPDPSS PPVTRVSPVPSPSQPARRLIRLSSLERLRLSSLNIIPDSGAEPPPKPPRSCSDLARESLKSSFVGWGVPV QAPQVPEAIEKGDDEEEEEEEEEEEEEPLPPLEPELEQTLLTLAKNPGAMTKYPTWRRTLMRRAKEEEMK RFCKAQAIQRRLNEIEATMRELEAEGTKLELALRKESSSPEQQKKLWLDQLLRLIQKKNSLVTEEAELMI TVQELDLEEKQRQLDHELRGYMNREETMKTEADLQSENQVLRKLLEVVNQRDALIQFQEERRLREMPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138315 |
| ORF Size | 3147 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138315.2, NP_612188.1 |
| RefSeq Size | 3563 bp |
| RefSeq ORF | 3147 bp |
| Locus ID | 171580 |
| UniProt ID | Q8VDP3 |
| MW | 116.8 kDa |
| Gene Summary | Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (By similarity). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels. May act as Rab effector protein and play a role in vesicle trafficking.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223357 | Mical1 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 1, (10ug) |
CNY 13,490.00 |
|
| MG211527 | Mical1 (tGFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1) |
CNY 12,552.00 |
|
| MR211527L3 | Lenti ORF clone of Mical1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 1 |
CNY 12,350.00 |
|
| MR211527L4 | Lenti ORF clone of Mical1 (mGFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 1 |
CNY 12,350.00 |
