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Phf20 (BC060121) Mouse Tagged ORF Clone
CAT＃: MR211469
- TrueORF®
Phf20 (Myc-DDK-tagged) - Mouse PHD finger protein 20 (cDNA clone MGC:76344 IMAGE:6413021)
ORF Plasmid: tGFP
"BC060121" in other vectors (4)
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CNY 7,128.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6820402O20Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211469 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAGCACCCACCTAACAGACGAGGAATCAGCTTTGAAGTGGGAGCCCAGTTGGAAGCTCGGGACC GTTTAAAAAACTGGTATCCAGCTCATATTGAAGACATTGACTACGAAGAAGGGAGAGTTCTCATCCATTT CAAGCGTTGGAACCATCGTTACGATGAGTGGTTTTGCTGGGACAGTCCTTATCTGCGCCCTTTAGAGAAG ATCCAGCTGAGGAAAGAGGGTTTACATGATGAGGATGGCTCTTCTGAATTTCAAATAAACCAGCAAGTGC TTGCTTGCTGGTCTGACTGTCGATTTTATCCAGCCAGAGTCACTGCTGTGAACAAGGATGGTACTTACAC TGTGAAATTTTATGATGGAGTAGTTCAAACTGTCAAACATATTCATGTCAAAGCTTTTTCCAAAGATCAG AATATTGTGGGTAATGCTAGGCCTAAAGAAACAGACCACAAAAGTCTTTCATCGTCTCCTGAGAAACGAG AGAAGTTTAAAGAACAGAGAAAAGTCACGGTCAATGTGAAGAAAGACAAAGTGGAAAAAGCCTTAAAGAC AGAAAAGCGGCCCAAGCAACCTGACAAAGAGGGAAAGCTGATCTGCTCAGAAAAAGGCAAAGTGTCAGAG AAAAGCCTTCCTAAGAACGAAAAGGAAGATAAGGAGAACATTTCCGAGAACGAGCGGGAGTACTCCGGGG ATGCCCAGGTGGAAAAGAAGCCTGAGAAGGACCTTGTGAAGAACCCACAAGAGAACCTGAAGGAGCCAAA AAGAAAACGAGGCAGACCCCCTTCCATAACTCCTACGGCTGTGGATTCAAACTCTCAAACTTTGCAACCA ATAACATTGGAATTGAGAAGACGGAAAATATCAAAACGAAGTGACACCCCATTAAAGCGTCCCAGACTCG ACAAAAATTCACCCCAGGAACAGTCAAAAAAACGCTCTGAAAATAGTGACAAAGACTTATCCAGGAGACG GTCCTCCAGGCTGTCCACTAATGGGACCCGTGAGATCCTAGATCCTGACTCGATTGTACCTGATCTGGTT CATACGGTTGATACAAACCCTCTACCAGACAAGTCACCCAGTGCCAAGGATTCTGCTGAAGGTCAGTTGA AGTCTCCATTGGAAGCTGGCCAGGTCTCTTCTGCATTAACTTGCCACCCCATTGGGGATGGCCTGGGGGC AGCAGATTTGGAGTTGAATTGCAAGTCAATGGGAGAAAACACGATGAAAACAGAACCTGTTTCTCCTCTT GCTGAGGTGCAGGAAGTTTCAACTGTTGAAGTTCCAAATACTTTGAAGAAAGTTGATGACTCTGTGACGT TGAATGTGCCAGCTGTGGACCTAGACCACAAGTTTCGATGCAAGGTTCTGGACTGTTTGAAATTTTTCCG CAAGGCTAAATTGCTGCACTATCATATGAAGTATTTCCATGGGATGGAGAAGTCACCAGAGCCAGAGGAG GGCCCAGGGAAGACGCATGTACAGACTCGGGGCTCTGCGGTGCCTGACAAGACAAGCCAGGAGAGCCTAA CCAGGAAGCGGGTCTCTGCCAGTTCCCCCACTGCAAAAGAGAAGGAAAAGACTAAAGAGAAGAAATTCAA AGAACTTGTGAGAGTGAAGCCAAAGAAGAAGAAATTCAAAGAACTTGTGAGAGTGAAGCCAAAGAAGAAA AAGAAAAAGAAAAAGAAAACCAAGCCTGAATGTCCCTGCAGTGAGGACATCAGTGATACTTCCCAGGAAC CTTCTCCACCCAAAACATTTGCTGTCACCAGGTGTGGGTCCTCACACAAGCCTGGGGTCCATATGAGCCC GCAGCTCCATGGTTCAGATAATGGAAACCACAAAGGGAAATTGAAAACCTGTGAGGAGGATAATTTGAGT GAGTCCTCTTCCGAGAGCTTTCTTTGGAGTGATGAGGAATATGGTCAAGATGTTGATGTGACCACCAACC CAGATGAGGAGCTTGAGGGCGACGACCGCTATGATTTTGAGGTGGTCCGCTGCATCTGTGAAGTGCAGGA GGAAAATGACTTCATGATTCAGTGTGAAGAGTGCCAGTGCTGGCAGCACGGGGTCTGCATGGGCTTACTG GAAGAAAACGTGCCTGAGAAATACACCTGCTATGTTTGCCAAGACCCTCCAGGTCAGAGGCCTGGCTTCA AGTACTGGTATGACAAGGAGTGGCTGAGCCGGGGACACATGCATGGTCTGGCATTTCTGGATCAGAACTA CTCCCACCAGAATGCCAGGAAGATCGTGGCCACCCACCAGCTGCTCGGGGACGTGCAGAGAGTGATCCAA GTGCTGCACGGCCTGCAGCTCAAGATGAGCATTCTGCAAAGCAGAGAGCATCCTGATCTGCAGCTATGGT GCCAGCCCTGGAAACAGCACTCGGGGGAGGGAAGAGCACATCCCAGACACATCCACATCACAGACGCCAG GAGCGAGGAGTCCCCAAGCTATAGAACTTTGAATGGGGCGGTGGAGAAGCCATCGCCCCTGCCCCGATCT GTGGAGGAGTCTTACATCACCAGTGAGCATTGCTACCAGAAACCTCGCGCCTATTATCCCGCTGTGGAGC AGAGGCTGGTCGTGGAGACGAGAGGCTCTGCCCTTGATGCTGCAGTCAGCCCCCTCTGTGAGAACGGCGA CGACTCCCTCTCCCCTCGCCTAGGCTGGCCCATCGACCAAGACAGGAGCAGAGGCGACATAGATCCCAAA CCCAGCTCCCCAAAGGTGAGAGAGTACATTTCCAAGAATGTCTTGCCAGAAGAGACGCCTGCAAGGAAGC TGCTGGACAGAGGTGGAGAAGGGCTGGTGAGCTCTCAGCACCAGTGGCAGTTCAACCTGCTCACACATGT GGAGTCCCTGCAGGATGAGGTGACGCACCGGATGGACTCCATTGAGAAGGAGCTGGACGTGCTGGAGAGC TGGCTGGACTACACTGGGGAGCTGGAGCCCCCAGAGCCACTGGCCAGGCTTCCGCAGCTCAAGCACTGCA TCAAGCAGCTGCTGACTGACCTGGGCAAGGTGCAGCAGATCGCCCTCTGCTGCTCGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211469 protein sequence
Red=Cloning site Green=Tags(s) MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGRVLIHFKRWNHRYDEWFCWDSPYLRPLEK IQLRKEGLHDEDGSSEFQINQQVLACWSDCRFYPARVTAVNKDGTYTVKFYDGVVQTVKHIHVKAFSKDQ NIVGNARPKETDHKSLSSSPEKREKFKEQRKVTVNVKKDKVEKALKTEKRPKQPDKEGKLICSEKGKVSE KSLPKNEKEDKENISENEREYSGDAQVEKKPEKDLVKNPQENLKEPKRKRGRPPSITPTAVDSNSQTLQP ITLELRRRKISKRSDTPLKRPRLDKNSPQEQSKKRSENSDKDLSRRRSSRLSTNGTREILDPDSIVPDLV HTVDTNPLPDKSPSAKDSAEGQLKSPLEAGQVSSALTCHPIGDGLGAADLELNCKSMGENTMKTEPVSPL AEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEE GPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKFKELVRVKPKKK KKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLS ESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLL EENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQ VLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRS VEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPK PSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLES WLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060121 |
| ORF Size | 3069 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060121, AAH60121 |
| RefSeq Size | 5665 bp |
| RefSeq ORF | 3071 bp |
| Locus ID | 228829 |
| MW | 116.9 kDa |
| Gene Summary | Contributes to methyllysine-dependent p53/TP53 stabilization and up-regulation after DNA damage (By similarity). Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223127 | Phf20 (untagged) - Mouse PHD finger protein 20 (cDNA clone MGC:76344 IMAGE:6413021), (10ug) |
CNY 12,920.00 |
|
| MG211469 | Phf20 (tGFP-tagged) - Mouse PHD finger protein 20 (cDNA clone MGC:76344 IMAGE:6413021) |
CNY 6,370.00 |
|
| MR211469L1 | Lenti ORF clone of Phf20 (Myc-DDK-tagged) - Mouse PHD finger protein 20 (cDNA clone MGC:76344 IMAGE:6413021) |
CNY 7,700.00 |
|
| MR211469L2 | Lenti ORF clone of Phf20 (mGFP-tagged) - Mouse PHD finger protein 20 (cDNA clone MGC:76344 IMAGE:6413021) |
CNY 7,700.00 |
