Egflam (BC095994) Mouse Tagged ORF Clone

CAT#: MR211456

  • TrueORF®

Egflam (Myc-DDK-tagged) - Mouse EGF-like, fibronectin type III and laminin G domains (cDNA clone MGC:106114 IMAGE:6467602)

ORF Plasmid: DDK tGFP



  "BC095994" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 10,170.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 5930412K08; AU040377
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211456 representing BC095994
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTTAATCTCAACCTTCTCGCTCCATTTCTTGCTCCTGGCCTGCAGCCTCCCACCCGGCGCTGTGT
CACTCCGAACGGCTCTGCGAAAATCAGGCAAGGTGGGACCTCCTCTTGACATCAAGCTGGGAGCCTTGAA
CTGTACAGCTTTCAGCATCCAGTGGAAAACACCAAAGCGTTCTGGTAGCTCCATCATTGGCTACACAGTG
TTTTACTCTGAGGTTGGTTCAGATAAGTCCCTGCGGGAGCGGTCACACAACGTCCCGGTTGGCCAGGATA
CCCTAATCACTGAGGAAGTGATTGGAGATTTGAAGCCAGGCACTGAATATCAAGTAAGCGTAGCAGCCTA
CAGTCAGACTGGCAAGGGGAGGCTTAGCTTCCCTCGACATGTGACCACTTTGTCTCAAGATTCCTGCCTG
CCCCCTGCAGCACCCCAACAGCCACATGTCCTTGTGGTTTCCGATTCTGAAGTGGCCCTTTCTTGGAGAC
CTGGAGAGAATGAAGGAAGTGCCCCAATTCAGTCCTACTCTGTGGAATTCATTAGACCAGATTTTGACAA
GAGCTGGACCATAATCCAAGAGCGCCTGCAGATGGACTCCATGGTTATCAAAGGCCTTGATCCAGATACC
AACTACCAGTTTGCTGTGAAAGCCATGAATGCTCACGGCTTCAGTCCGCGCAGCTGGCCCAGCAACACCG
TCCGAACCCTGGGTCCCGGGGAGGCTGGAAGTGGTCATTATGGACCTGGGTATATCACCAATCCCGGTGT
GAGTGAAGATGATGATGGATCTGAAGACGAACTGGACCTGGATGTATCCTTTGAGGAGGTTAAACCACTT
CCTGCTACCAAAGTTGGGAATAAGAAGTTCTCAGTGGAGTCCAAGAAGACTTCTGTCTCCAACTCAGTGA
TGGGCTCCAGACTCGCCCAACCTACCTCAGCCTCTCTCCACGAAACTACAGTGGCCATCCCACCCACACC
AGCCCAACGGAAGGGGAAGAACAGTGTGGCCATGATGTCAAGGCTCTTTGACATGTCCTGTGATGAAACT
CTCTGTTCAGCTGACAGCTTCTGTGTCAATGACTACGCCTGGGGGGGCTCACGATGCCATTGCAACCTGG
GCAAAGGAGGCGAGGCCTGCTCAGAAGATATCTTTATTCAGTATCCTCAGTTCTTTGGCCACTCCTACGT
AACCTTTGAGCCTCTGAAGAACTCTTATCAGGCATTTCAGGTTACTCTGGAGTTCAGGGCAGAGGCGGAG
GATGGCTTGCTGCTCTACTGCGGAGAGAGTGAGCACGGAAGAGGAGACTTCATGTCCCTGGCTCTCATCC
GGCGGTCCCTCCACTTCAGGTTTAATTGTGGAACTGGGATTGCCATCATCATAAGTGAGACCAAAATCAA
ACTTGGGGCCTGGCATACGGTGACACTGTACAGAGATGGCCTGAATGGGATGCTGCAGTTGAATAATGGC
ACCCCAGTGACAGGCCAGTCCCAGGGTCAGTACAGTAAAATTACCTTCCGGACACCTCTGTACCTCGGGG
GCGCTCCCAGTGCTTACTGGCTGGTCAGAGCAACAGGGACAAACCGTGGCTTCCAAGGCTGTGTGCAGTC
ACTTTCTGTTAATGGGAAGAAGATTGACATGAGGCCCTGGCCATTAGGAAAAGCCCTCAATGGAGCTGAT
GTTGGGGAGTGCAGCAGTGGGATCTGTGACGAAGCCTCCTGCATCCACGGGGGCACCTGCGCAGCCATCA
AGGCCGACTCCTACATCTGCCTCTGTCCCCTTGGGTTCAGAGGTCGACACTGTGAAGATGCTTTTGCCCT
GACCATCCCTCAGTTCAGAGAGTCGCTGAGGTCCTATGCTGCCACACCCTGGCCCCTGGAGCCTCAGCAT
TATCTTTCTTTCACCGAGTTCGAGATCACCTTTCGGCCAGACTCGGGGGATGGTGTCCTTTTGTACAGCT
ACGACACGGGCAGCAAGGACTTCCTCTCCATCAATATGGCTGCGGGCCACGTGGAATTCCGCTTCGACTG
TGGTTCTGGGACGGGAGTTCTCAGGAGTGAGGCTCCCCTCACCCTAGGCCAATGGCATGACCTACGTGTG
TCTCGCACAGCGAAGAATGGGATCTTACAGGTGGACAAGCAGAAGGTGGTAGAGGGAATGGCAGAGGGAG
GCTTCACCCAGATTAAGTGTAATACGGACATTTTTATTGGTGGAGTCCCAAATTACGATGATGTCAAGAA
GAACTCGGGGATCCTCCATCCATTTAGTGGGAGCATTCAGAAGATCATCCTCAACGACCGGACCATCCAT
GTGAAGCATGACTTTACATCGGGCGTGAATGTGGAGAACGCAGCCCACCCCTGTGTAGGGGCCCCCTGTG
CCCATGGGGGCAGCTGTCGGCCCAGGAAGGAGGGTTATGAGTGCGACTGTCCCTTGGGCTTCGAAGGCCT
GAACTGCCAGAAAGAGTGTGGGAACCACTGCCTCAATACTATTATAGAAGCCATCGAGATCCCACAGTTC
ATCGGCCGCAGCTACCTGACGTATGACAACCCAAATATCCTCAAGAGGGTGTCTGGATCGAGGTCAAATG
CGTTCATGAGGTTTAAGACAACCGCCAAGGATGGCCTGTTGCTGTGGAGGGGGGACAGCCCCATGAGACC
TAACAGTGACTTCATTTCCCTGGGTCTTCGGGATGGAGCCCTGATATTCAGCTACAACCTGGGCAGTGGC
GTGGCTTCCATCATGGTGAACGGTTCCTTCAGCGATGGCCGGTGGCACCGAGTCAAGGCTGTCAGGGATG
GCCAGTCCGGGAAGATTACAGTGGATGACTACGGAGCCAGAACAGGCAAGTCACCGGGCTTGATGCGGCA
GCTTAACATCAACGGTGCTCTGTACGTGGGTGGAATGAAGGAAATCGCTCTGCACACGAACAGACAATAC
CTGCGAGGCCTTGTGGGCTGCATCTCCCATTTCACACTGTCCACCGATTACCACATTTCCCTCGTGGAAG
ACGCCGTGGATGGGAAGAACATCAACACTTGTGGGGCTAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211456 representing BC095994
Red=Cloning site Green=Tags(s)

MDLISTFSLHFLLLACSLPPGAVSLRTALRKSGKVGPPLDIKLGALNCTAFSIQWKTPKRSGSSIIGYTV
FYSEVGSDKSLRERSHNVPVGQDTLITEEVIGDLKPGTEYQVSVAAYSQTGKGRLSFPRHVTTLSQDSCL
PPAAPQQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDT
NYQFAVKAMNAHGFSPRSWPSNTVRTLGPGEAGSGHYGPGYITNPGVSEDDDGSEDELDLDVSFEEVKPL
PATKVGNKKFSVESKKTSVSNSVMGSRLAQPTSASLHETTVAIPPTPAQRKGKNSVAMMSRLFDMSCDET
LCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRAEAE
DGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNG
TPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGAD
VGECSSGICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQH
YLSFTEFEITFRPDSGDGVLLYSYDTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRV
SRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIH
VKHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQF
IGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSG
VASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEIALHTNRQY
LRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC095994
ORF Size 3051 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC095994.1
RefSeq Size 4102 bp
RefSeq ORF 3053 bp
Locus ID 268780
MW 150.4 kDa
Gene Summary Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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