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Egflam (BC095994) Mouse Tagged ORF Clone
CAT＃: MR211456
- TrueORF®
Egflam (Myc-DDK-tagged) - Mouse EGF-like, fibronectin type III and laminin G domains (cDNA clone MGC:106114 IMAGE:6467602)
ORF Plasmid: tGFP
"BC095994" in other vectors (4)
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CNY 10,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5930412K08; AU040377 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211456 representing BC095994
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTAATCTCAACCTTCTCGCTCCATTTCTTGCTCCTGGCCTGCAGCCTCCCACCCGGCGCTGTGT CACTCCGAACGGCTCTGCGAAAATCAGGCAAGGTGGGACCTCCTCTTGACATCAAGCTGGGAGCCTTGAA CTGTACAGCTTTCAGCATCCAGTGGAAAACACCAAAGCGTTCTGGTAGCTCCATCATTGGCTACACAGTG TTTTACTCTGAGGTTGGTTCAGATAAGTCCCTGCGGGAGCGGTCACACAACGTCCCGGTTGGCCAGGATA CCCTAATCACTGAGGAAGTGATTGGAGATTTGAAGCCAGGCACTGAATATCAAGTAAGCGTAGCAGCCTA CAGTCAGACTGGCAAGGGGAGGCTTAGCTTCCCTCGACATGTGACCACTTTGTCTCAAGATTCCTGCCTG CCCCCTGCAGCACCCCAACAGCCACATGTCCTTGTGGTTTCCGATTCTGAAGTGGCCCTTTCTTGGAGAC CTGGAGAGAATGAAGGAAGTGCCCCAATTCAGTCCTACTCTGTGGAATTCATTAGACCAGATTTTGACAA GAGCTGGACCATAATCCAAGAGCGCCTGCAGATGGACTCCATGGTTATCAAAGGCCTTGATCCAGATACC AACTACCAGTTTGCTGTGAAAGCCATGAATGCTCACGGCTTCAGTCCGCGCAGCTGGCCCAGCAACACCG TCCGAACCCTGGGTCCCGGGGAGGCTGGAAGTGGTCATTATGGACCTGGGTATATCACCAATCCCGGTGT GAGTGAAGATGATGATGGATCTGAAGACGAACTGGACCTGGATGTATCCTTTGAGGAGGTTAAACCACTT CCTGCTACCAAAGTTGGGAATAAGAAGTTCTCAGTGGAGTCCAAGAAGACTTCTGTCTCCAACTCAGTGA TGGGCTCCAGACTCGCCCAACCTACCTCAGCCTCTCTCCACGAAACTACAGTGGCCATCCCACCCACACC AGCCCAACGGAAGGGGAAGAACAGTGTGGCCATGATGTCAAGGCTCTTTGACATGTCCTGTGATGAAACT CTCTGTTCAGCTGACAGCTTCTGTGTCAATGACTACGCCTGGGGGGGCTCACGATGCCATTGCAACCTGG GCAAAGGAGGCGAGGCCTGCTCAGAAGATATCTTTATTCAGTATCCTCAGTTCTTTGGCCACTCCTACGT AACCTTTGAGCCTCTGAAGAACTCTTATCAGGCATTTCAGGTTACTCTGGAGTTCAGGGCAGAGGCGGAG GATGGCTTGCTGCTCTACTGCGGAGAGAGTGAGCACGGAAGAGGAGACTTCATGTCCCTGGCTCTCATCC GGCGGTCCCTCCACTTCAGGTTTAATTGTGGAACTGGGATTGCCATCATCATAAGTGAGACCAAAATCAA ACTTGGGGCCTGGCATACGGTGACACTGTACAGAGATGGCCTGAATGGGATGCTGCAGTTGAATAATGGC ACCCCAGTGACAGGCCAGTCCCAGGGTCAGTACAGTAAAATTACCTTCCGGACACCTCTGTACCTCGGGG GCGCTCCCAGTGCTTACTGGCTGGTCAGAGCAACAGGGACAAACCGTGGCTTCCAAGGCTGTGTGCAGTC ACTTTCTGTTAATGGGAAGAAGATTGACATGAGGCCCTGGCCATTAGGAAAAGCCCTCAATGGAGCTGAT GTTGGGGAGTGCAGCAGTGGGATCTGTGACGAAGCCTCCTGCATCCACGGGGGCACCTGCGCAGCCATCA AGGCCGACTCCTACATCTGCCTCTGTCCCCTTGGGTTCAGAGGTCGACACTGTGAAGATGCTTTTGCCCT GACCATCCCTCAGTTCAGAGAGTCGCTGAGGTCCTATGCTGCCACACCCTGGCCCCTGGAGCCTCAGCAT TATCTTTCTTTCACCGAGTTCGAGATCACCTTTCGGCCAGACTCGGGGGATGGTGTCCTTTTGTACAGCT ACGACACGGGCAGCAAGGACTTCCTCTCCATCAATATGGCTGCGGGCCACGTGGAATTCCGCTTCGACTG TGGTTCTGGGACGGGAGTTCTCAGGAGTGAGGCTCCCCTCACCCTAGGCCAATGGCATGACCTACGTGTG TCTCGCACAGCGAAGAATGGGATCTTACAGGTGGACAAGCAGAAGGTGGTAGAGGGAATGGCAGAGGGAG GCTTCACCCAGATTAAGTGTAATACGGACATTTTTATTGGTGGAGTCCCAAATTACGATGATGTCAAGAA GAACTCGGGGATCCTCCATCCATTTAGTGGGAGCATTCAGAAGATCATCCTCAACGACCGGACCATCCAT GTGAAGCATGACTTTACATCGGGCGTGAATGTGGAGAACGCAGCCCACCCCTGTGTAGGGGCCCCCTGTG CCCATGGGGGCAGCTGTCGGCCCAGGAAGGAGGGTTATGAGTGCGACTGTCCCTTGGGCTTCGAAGGCCT GAACTGCCAGAAAGAGTGTGGGAACCACTGCCTCAATACTATTATAGAAGCCATCGAGATCCCACAGTTC ATCGGCCGCAGCTACCTGACGTATGACAACCCAAATATCCTCAAGAGGGTGTCTGGATCGAGGTCAAATG CGTTCATGAGGTTTAAGACAACCGCCAAGGATGGCCTGTTGCTGTGGAGGGGGGACAGCCCCATGAGACC TAACAGTGACTTCATTTCCCTGGGTCTTCGGGATGGAGCCCTGATATTCAGCTACAACCTGGGCAGTGGC GTGGCTTCCATCATGGTGAACGGTTCCTTCAGCGATGGCCGGTGGCACCGAGTCAAGGCTGTCAGGGATG GCCAGTCCGGGAAGATTACAGTGGATGACTACGGAGCCAGAACAGGCAAGTCACCGGGCTTGATGCGGCA GCTTAACATCAACGGTGCTCTGTACGTGGGTGGAATGAAGGAAATCGCTCTGCACACGAACAGACAATAC CTGCGAGGCCTTGTGGGCTGCATCTCCCATTTCACACTGTCCACCGATTACCACATTTCCCTCGTGGAAG ACGCCGTGGATGGGAAGAACATCAACACTTGTGGGGCTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211456 representing BC095994
Red=Cloning site Green=Tags(s) MDLISTFSLHFLLLACSLPPGAVSLRTALRKSGKVGPPLDIKLGALNCTAFSIQWKTPKRSGSSIIGYTV FYSEVGSDKSLRERSHNVPVGQDTLITEEVIGDLKPGTEYQVSVAAYSQTGKGRLSFPRHVTTLSQDSCL PPAAPQQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDT NYQFAVKAMNAHGFSPRSWPSNTVRTLGPGEAGSGHYGPGYITNPGVSEDDDGSEDELDLDVSFEEVKPL PATKVGNKKFSVESKKTSVSNSVMGSRLAQPTSASLHETTVAIPPTPAQRKGKNSVAMMSRLFDMSCDET LCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRAEAE DGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNG TPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGAD VGECSSGICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQH YLSFTEFEITFRPDSGDGVLLYSYDTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRV SRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIH VKHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQF IGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSG VASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEIALHTNRQY LRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC095994 |
| ORF Size | 3051 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC095994.1 |
| RefSeq Size | 4102 bp |
| RefSeq ORF | 3053 bp |
| Locus ID | 268780 |
| MW | 150.4 kDa |
| Gene Summary | Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223135 | Egflam (untagged) - Mouse EGF-like, fibronectin type III and laminin G domains (cDNA clone MGC:106114 IMAGE:6467602), (10ug) |
CNY 12,920.00 |
|
| MG211456 | Egflam (tGFP-tagged) - Mouse EGF-like, fibronectin type III and laminin G domains (cDNA clone MGC:106114 IMAGE:6467602) |
CNY 11,210.00 |
|
| MR211456L3 | Lenti ORF clone of Egflam (Myc-DDK-tagged) - Mouse EGF-like, fibronectin type III and laminin G domains (cDNA clone MGC:106114 IMAGE:6467602) |
CNY 12,070.00 |
|
| MR211456L4 | Lenti ORF clone of Egflam (mGFP-tagged) - Mouse EGF-like, fibronectin type III and laminin G domains (cDNA clone MGC:106114 IMAGE:6467602) |
CNY 12,070.00 |
