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Plekha7 (BC084587) Mouse Tagged ORF Clone
CAT＃: MR211446
- TrueORF®
Plekha7 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family A member 7 (cDNA clone MGC:90927 IMAGE:3602481)
ORF Plasmid: tGFP
"BC084587" in other vectors (4)
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CNY 7,056.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A430081P20Rik; AI414265 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211446 representing BC084587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAGCCAGAGAGAAACCAAAGACCGTCCAGCATGGTCAGTGAGACATCCACAGCTGGGACCACGT CCACCCTGGAGGCCAAGCCTGGACCCAAGATTGTCAAGTCCAGCAGTAAAGTGCACAGCTTCGGGAAGAG GGACCAGGCCATTCGGAGGAACCTTAATGTTCCGGTGGTGGTGAGGGGCTGGCTACACAAGCAGGACAGC TCCGGGATGAGGCTGTGGAAAAGACGCTGGTTTGTGCTGGCTGATTACTGCTTGTTTTATTATAAAGACA GCCGAGAGGAAGCTGTTCTCGGGAGCATCCCTCTGCCCAGCTATGTCATCTCCCCAGTGGCCCCAGAGGA TCGAATAAGCCGCAAGTATTCCTTTAAGGCTGTGCATACGGGGATGAGGGCACTCATCTACAGCACCACC ACAGCGGGCTCTCAGATGGAGCACTCGGGCATGAGGACCTACTACTTCAGTGCCGACACCCTGGAGGACA TGAACGCCTGGGTCAGGGCCATGAACCAGGCTGCGCAGGTGCTGTCTCGCTCATCGCTGAGGAGGGACGT GGACAAGGTGGAGCGGCAGGCCATGCCACAGGCCAACCACACTGACGCCTGCCAAGAATGTGGCCACGTG GGACCTGGGCACTCGAGAGACTGTCCTCGTCGAGGCTATGAGGACTCCTATGGCTTCAACAGGAGGGAGC AGGAGGAGGAACGCTTCCGTGCTCAAAGAGACCCACTGGAGGGCAGGAGGGACAGGAGCAAGGCCAGGTC CCCATACTTGCCAGCTGAGGAGGATGCCTTGTTTGTCGATCTACCTGGTGGGCCCCGAGGCCAGCAAGCA CAGCCACAGCGAGCCGAGAAGAACGGGGTGCCACCTTATGGCCTGGGAGAACAGAATGGGACCAATGGGT ACCAGCGGACTGCTCCTCCCAGGGCCAACCCAGAAAAACACAGCCAGAGGAAGACCGGCCTGGCCCAGGC AGAGCACTGGACAAAAGCTCAGAAGGGGGACGGCAGGAGCTTGCCCTTAGACCAAACACTTCCTCGCCAG GGTCCCAGCCAGCCCCTGTCCTTCCCGGAAAACTACCAGAGTCTTCCCAAGAGCACTAGACATCTTTCTG GGAGCTCGTCACCCCCTCCCCGGAACCTGCCCAGCGACTACAAGTACGCACAGGACCGAGCCAGCCACCT GAAGATGTCCAGTGAAGAGCGCCGGGCACACAGAGACGGCACTGTGTGGCAGCTCTATGAGTGGCAGCAG AGGCAGCAGTTCCGTCACGGCAGCCCCACCGCACCCATAGGAGCTGGCTCCCCAGAGTTCACCGAGCAGG GTCGCAGCCGGAGCCTGCTGGAGGTGCCCCGCTCCATCTCCGTGCCTCCGTCTCCTTCTGACATCCCTCC TCCTGGGCCTCCAAGGCCCTTTCCGCCCCGGCGGCCACATACACCAGCAGAAAGGGTCACAGTGAAGCCA CCCGAGCAGAGGAGGAGTGTGGACATCTCTCTGGGGGGTTCTCCCAGGAAGGCACGGGGTCATGCTGCCA AGAACTCCTCTCACGTGGACCGGCGTTCCATGCCCTCCATGGGCTACATGACCCATACAGTGAGCGCTCC CAGCTTACATGGAAAATCGGCTGATGACACCTACCTCCAGCTGAAGAAGGACCTGGAGTATCTGGATCTG AAGATGACAGGCCGAGACCTTCTTAAAGATCGAAGTCTGAAGCCCATGAAGATCGCCGAGAGTGACATTG ATGTCAAGCTGAGCATCTTCTGTGAGCAAGACAGGATACTCCAGGACTTGGAAGACAAGATCCGAGCTCT CAAGGAGAATAAAGATCAGCTAGAATCCGTGCTGGAGGTGCTGCACAGGCAGACAGAACAGTACCGAGAC CAGCCACAGCACCTGGAGAAGATCACTTGCCAGCAGAGGCTCCTGCAGGAGGACCTCGTCCACATCCGTG CGGAGCTCTGCAGAGAGTCCACGGAGATGGAAAATGCATGGAACGAGTACCTGAAGCTGGAGAAGGATGT GGAGCAGCTAAAGCAGACCCTGCAGGAGCAACACAGGAGAGCCTTTTTTTTCCAGGAGAAGTCTCAGATA CAGAAGGATCTGTGGAGGATCGAAGATGTCATGGCAGGCCTGAGTGCGAATAAAGAGAACTACAGAGTCC TGGTGGGATCTGTCAAAAATCCAGAAAGAAAAACGGTGCCTTTGTTCCCTCACCCATCTGTGCCTTCACT CTCTCCAACTGAGAGCAAGCCGGCCCTGCAGCCCAGCCCTCCCACCAGCCCCGTGCGGACGCCTCTGGAG GTCCGACTCTTTCCGCAGCTGCAGACCTATGTGCCATACCGACCTCACCCGCCCCAGCTGAGGAAAGTGA TGTCTCCTCTGCAGTCACCAACCAAGGCAAAGCCTCAGGCGGAAGACGAGGCACCTCCCAGGCCACCACT TCCTGAACTCTACAGCCCAGAGGACCAGCCCCCAGCTGTGCCACCACTGCCGAGAGAAGCCACCATCATT CGGCACACATCTGTGCGTGGACTCAAACGACAGTCGGATGAGAGGAAGAGGGACCGGGAGCAGGGCCAGT GTGTGAATGGGGACTTGAAGGTGGAGCTCCGGTCCTACGTGAGTGAGCCAGAGCTGGCCAGCCTCAGTGG GGACGTGCCTCAGCCCTCCTTGAGCCTCGTGGGCTCTGAGAGCAGGTACCAGACGCTGCCAGGCAGAGGG CTCTCAGGCTCCACGTCCAGGCTCCAGCAGTCGTCCACCATTGCTCCCTATGTCACACTCCGGAGGGGGC TGAATGCCGAAAACAGCAGTGCGACCTTCTCTAGACCCAAGAGTGCCTTGGAGCGTCTGTACTCGGGGGA CCACCAGCGGGGCAAGATGAGTGCGGAGGAACAGCTGGAGCGGATGAAGCGGCACCAGAAGGCTCTGGTC CGAGAGCGCAAGAGGACCCTGAGCCAAGGAGAAAAGACGGGTCTGCTCTCAGCCCGATACCTCAGCCAGC CACTGCCAGGAGATCTCGGCTCAGTATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211446 representing BC084587
Red=Cloning site Green=Tags(s) MSKPERNQRPSSMVSETSTAGTTSTLEAKPGPKIVKSSSKVHSFGKRDQAIRRNLNVPVVVRGWLHKQDS SGMRLWKRRWFVLADYCLFYYKDSREEAVLGSIPLPSYVISPVAPEDRISRKYSFKAVHTGMRALIYSTT TAGSQMEHSGMRTYYFSADTLEDMNAWVRAMNQAAQVLSRSSLRRDVDKVERQAMPQANHTDACQECGHV GPGHSRDCPRRGYEDSYGFNRREQEEERFRAQRDPLEGRRDRSKARSPYLPAEEDALFVDLPGGPRGQQA QPQRAEKNGVPPYGLGEQNGTNGYQRTAPPRANPEKHSQRKTGLAQAEHWTKAQKGDGRSLPLDQTLPRQ GPSQPLSFPENYQSLPKSTRHLSGSSSPPPRNLPSDYKYAQDRASHLKMSSEERRAHRDGTVWQLYEWQQ RQQFRHGSPTAPIGAGSPEFTEQGRSRSLLEVPRSISVPPSPSDIPPPGPPRPFPPRRPHTPAERVTVKP PEQRRSVDISLGGSPRKARGHAAKNSSHVDRRSMPSMGYMTHTVSAPSLHGKSADDTYLQLKKDLEYLDL KMTGRDLLKDRSLKPMKIAESDIDVKLSIFCEQDRILQDLEDKIRALKENKDQLESVLEVLHRQTEQYRD QPQHLEKITCQQRLLQEDLVHIRAELCRESTEMENAWNEYLKLEKDVEQLKQTLQEQHRRAFFFQEKSQI QKDLWRIEDVMAGLSANKENYRVLVGSVKNPERKTVPLFPHPSVPSLSPTESKPALQPSPPTSPVRTPLE VRLFPQLQTYVPYRPHPPQLRKVMSPLQSPTKAKPQAEDEAPPRPPLPELYSPEDQPPAVPPLPREATII RHTSVRGLKRQSDERKRDREQGQCVNGDLKVELRSYVSEPELASLSGDVPQPSLSLVGSESRYQTLPGRG LSGSTSRLQQSSTIAPYVTLRRGLNAENSSATFSRPKSALERLYSGDHQRGKMSAEEQLERMKRHQKALV RERKRTLSQGEKTGLLSARYLSQPLPGDLGSVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC084587 |
| ORF Size | 3039 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC084587.1 |
| RefSeq Size | 4537 bp |
| RefSeq ORF | 3041 bp |
| Locus ID | 233765 |
| MW | 166.3 kDa |
| Gene Summary | Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to the recruitment of KIFC3 kinesin to the junctional site (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223130 | Plekha7 (untagged) - Mouse pleckstrin homology domain containing, family A member 7 (cDNA clone MGC:90927 IMAGE:3602481), (10ug) |
CNY 12,920.00 |
|
| MG211446 | Plekha7 (tGFP-tagged) - Mouse pleckstrin homology domain containing, family A member 7 (cDNA clone MGC:90927 IMAGE:3602481) |
CNY 6,370.00 |
|
| MR211446L3 | Lenti ORF clone of Plekha7 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family A member 7 (cDNA clone MGC:90927 IMAGE:3602481) |
CNY 7,700.00 |
|
| MR211446L4 | Lenti ORF clone of Plekha7 (mGFP-tagged) - Mouse pleckstrin homology domain containing, family A member 7 (cDNA clone MGC:90927 IMAGE:3602481) |
CNY 7,700.00 |
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