Plekha7 (BC084587) Mouse Tagged ORF Clone

CAT#: MR211446

  • TrueORF®

Plekha7 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family A member 7 (cDNA clone MGC:90927 IMAGE:3602481)

ORF Plasmid: DDK tGFP



  "BC084587" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 7,056.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms A430081P20Rik; AI414265
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211446 representing BC084587
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAAGCCAGAGAGAAACCAAAGACCGTCCAGCATGGTCAGTGAGACATCCACAGCTGGGACCACGT
CCACCCTGGAGGCCAAGCCTGGACCCAAGATTGTCAAGTCCAGCAGTAAAGTGCACAGCTTCGGGAAGAG
GGACCAGGCCATTCGGAGGAACCTTAATGTTCCGGTGGTGGTGAGGGGCTGGCTACACAAGCAGGACAGC
TCCGGGATGAGGCTGTGGAAAAGACGCTGGTTTGTGCTGGCTGATTACTGCTTGTTTTATTATAAAGACA
GCCGAGAGGAAGCTGTTCTCGGGAGCATCCCTCTGCCCAGCTATGTCATCTCCCCAGTGGCCCCAGAGGA
TCGAATAAGCCGCAAGTATTCCTTTAAGGCTGTGCATACGGGGATGAGGGCACTCATCTACAGCACCACC
ACAGCGGGCTCTCAGATGGAGCACTCGGGCATGAGGACCTACTACTTCAGTGCCGACACCCTGGAGGACA
TGAACGCCTGGGTCAGGGCCATGAACCAGGCTGCGCAGGTGCTGTCTCGCTCATCGCTGAGGAGGGACGT
GGACAAGGTGGAGCGGCAGGCCATGCCACAGGCCAACCACACTGACGCCTGCCAAGAATGTGGCCACGTG
GGACCTGGGCACTCGAGAGACTGTCCTCGTCGAGGCTATGAGGACTCCTATGGCTTCAACAGGAGGGAGC
AGGAGGAGGAACGCTTCCGTGCTCAAAGAGACCCACTGGAGGGCAGGAGGGACAGGAGCAAGGCCAGGTC
CCCATACTTGCCAGCTGAGGAGGATGCCTTGTTTGTCGATCTACCTGGTGGGCCCCGAGGCCAGCAAGCA
CAGCCACAGCGAGCCGAGAAGAACGGGGTGCCACCTTATGGCCTGGGAGAACAGAATGGGACCAATGGGT
ACCAGCGGACTGCTCCTCCCAGGGCCAACCCAGAAAAACACAGCCAGAGGAAGACCGGCCTGGCCCAGGC
AGAGCACTGGACAAAAGCTCAGAAGGGGGACGGCAGGAGCTTGCCCTTAGACCAAACACTTCCTCGCCAG
GGTCCCAGCCAGCCCCTGTCCTTCCCGGAAAACTACCAGAGTCTTCCCAAGAGCACTAGACATCTTTCTG
GGAGCTCGTCACCCCCTCCCCGGAACCTGCCCAGCGACTACAAGTACGCACAGGACCGAGCCAGCCACCT
GAAGATGTCCAGTGAAGAGCGCCGGGCACACAGAGACGGCACTGTGTGGCAGCTCTATGAGTGGCAGCAG
AGGCAGCAGTTCCGTCACGGCAGCCCCACCGCACCCATAGGAGCTGGCTCCCCAGAGTTCACCGAGCAGG
GTCGCAGCCGGAGCCTGCTGGAGGTGCCCCGCTCCATCTCCGTGCCTCCGTCTCCTTCTGACATCCCTCC
TCCTGGGCCTCCAAGGCCCTTTCCGCCCCGGCGGCCACATACACCAGCAGAAAGGGTCACAGTGAAGCCA
CCCGAGCAGAGGAGGAGTGTGGACATCTCTCTGGGGGGTTCTCCCAGGAAGGCACGGGGTCATGCTGCCA
AGAACTCCTCTCACGTGGACCGGCGTTCCATGCCCTCCATGGGCTACATGACCCATACAGTGAGCGCTCC
CAGCTTACATGGAAAATCGGCTGATGACACCTACCTCCAGCTGAAGAAGGACCTGGAGTATCTGGATCTG
AAGATGACAGGCCGAGACCTTCTTAAAGATCGAAGTCTGAAGCCCATGAAGATCGCCGAGAGTGACATTG
ATGTCAAGCTGAGCATCTTCTGTGAGCAAGACAGGATACTCCAGGACTTGGAAGACAAGATCCGAGCTCT
CAAGGAGAATAAAGATCAGCTAGAATCCGTGCTGGAGGTGCTGCACAGGCAGACAGAACAGTACCGAGAC
CAGCCACAGCACCTGGAGAAGATCACTTGCCAGCAGAGGCTCCTGCAGGAGGACCTCGTCCACATCCGTG
CGGAGCTCTGCAGAGAGTCCACGGAGATGGAAAATGCATGGAACGAGTACCTGAAGCTGGAGAAGGATGT
GGAGCAGCTAAAGCAGACCCTGCAGGAGCAACACAGGAGAGCCTTTTTTTTCCAGGAGAAGTCTCAGATA
CAGAAGGATCTGTGGAGGATCGAAGATGTCATGGCAGGCCTGAGTGCGAATAAAGAGAACTACAGAGTCC
TGGTGGGATCTGTCAAAAATCCAGAAAGAAAAACGGTGCCTTTGTTCCCTCACCCATCTGTGCCTTCACT
CTCTCCAACTGAGAGCAAGCCGGCCCTGCAGCCCAGCCCTCCCACCAGCCCCGTGCGGACGCCTCTGGAG
GTCCGACTCTTTCCGCAGCTGCAGACCTATGTGCCATACCGACCTCACCCGCCCCAGCTGAGGAAAGTGA
TGTCTCCTCTGCAGTCACCAACCAAGGCAAAGCCTCAGGCGGAAGACGAGGCACCTCCCAGGCCACCACT
TCCTGAACTCTACAGCCCAGAGGACCAGCCCCCAGCTGTGCCACCACTGCCGAGAGAAGCCACCATCATT
CGGCACACATCTGTGCGTGGACTCAAACGACAGTCGGATGAGAGGAAGAGGGACCGGGAGCAGGGCCAGT
GTGTGAATGGGGACTTGAAGGTGGAGCTCCGGTCCTACGTGAGTGAGCCAGAGCTGGCCAGCCTCAGTGG
GGACGTGCCTCAGCCCTCCTTGAGCCTCGTGGGCTCTGAGAGCAGGTACCAGACGCTGCCAGGCAGAGGG
CTCTCAGGCTCCACGTCCAGGCTCCAGCAGTCGTCCACCATTGCTCCCTATGTCACACTCCGGAGGGGGC
TGAATGCCGAAAACAGCAGTGCGACCTTCTCTAGACCCAAGAGTGCCTTGGAGCGTCTGTACTCGGGGGA
CCACCAGCGGGGCAAGATGAGTGCGGAGGAACAGCTGGAGCGGATGAAGCGGCACCAGAAGGCTCTGGTC
CGAGAGCGCAAGAGGACCCTGAGCCAAGGAGAAAAGACGGGTCTGCTCTCAGCCCGATACCTCAGCCAGC
CACTGCCAGGAGATCTCGGCTCAGTATGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211446 representing BC084587
Red=Cloning site Green=Tags(s)

MSKPERNQRPSSMVSETSTAGTTSTLEAKPGPKIVKSSSKVHSFGKRDQAIRRNLNVPVVVRGWLHKQDS
SGMRLWKRRWFVLADYCLFYYKDSREEAVLGSIPLPSYVISPVAPEDRISRKYSFKAVHTGMRALIYSTT
TAGSQMEHSGMRTYYFSADTLEDMNAWVRAMNQAAQVLSRSSLRRDVDKVERQAMPQANHTDACQECGHV
GPGHSRDCPRRGYEDSYGFNRREQEEERFRAQRDPLEGRRDRSKARSPYLPAEEDALFVDLPGGPRGQQA
QPQRAEKNGVPPYGLGEQNGTNGYQRTAPPRANPEKHSQRKTGLAQAEHWTKAQKGDGRSLPLDQTLPRQ
GPSQPLSFPENYQSLPKSTRHLSGSSSPPPRNLPSDYKYAQDRASHLKMSSEERRAHRDGTVWQLYEWQQ
RQQFRHGSPTAPIGAGSPEFTEQGRSRSLLEVPRSISVPPSPSDIPPPGPPRPFPPRRPHTPAERVTVKP
PEQRRSVDISLGGSPRKARGHAAKNSSHVDRRSMPSMGYMTHTVSAPSLHGKSADDTYLQLKKDLEYLDL
KMTGRDLLKDRSLKPMKIAESDIDVKLSIFCEQDRILQDLEDKIRALKENKDQLESVLEVLHRQTEQYRD
QPQHLEKITCQQRLLQEDLVHIRAELCRESTEMENAWNEYLKLEKDVEQLKQTLQEQHRRAFFFQEKSQI
QKDLWRIEDVMAGLSANKENYRVLVGSVKNPERKTVPLFPHPSVPSLSPTESKPALQPSPPTSPVRTPLE
VRLFPQLQTYVPYRPHPPQLRKVMSPLQSPTKAKPQAEDEAPPRPPLPELYSPEDQPPAVPPLPREATII
RHTSVRGLKRQSDERKRDREQGQCVNGDLKVELRSYVSEPELASLSGDVPQPSLSLVGSESRYQTLPGRG
LSGSTSRLQQSSTIAPYVTLRRGLNAENSSATFSRPKSALERLYSGDHQRGKMSAEEQLERMKRHQKALV
RERKRTLSQGEKTGLLSARYLSQPLPGDLGSVC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC084587
ORF Size 3039 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC084587.1
RefSeq Size 4537 bp
RefSeq ORF 3041 bp
Locus ID 233765
MW 166.3 kDa
Gene Summary Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to the recruitment of KIFC3 kinesin to the junctional site (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...