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Cep120 (NM_178686) Mouse Tagged ORF Clone
CAT＃: MR211393
- TrueORF®
Cep120 (Myc-DDK-tagged) - Mouse centrosomal protein 120 (Cep120)
ORF Plasmid: tGFP
"NM_178686" in other vectors (4)
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CNY 7,240.00
CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A230075C01; AU016693; Ccdc100 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211393 representing NM_178686
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCCCAAGTCCGACCAACTGCTCATCGTCGTATCCATCCTCGAAGGTCGCCATTTCCCCAAACGTC CAAAGCATCTGCTCGTGGTGGAAGCAAAGTTTGATGGGGAGCAGCTGGCTACTGATCCTGTCGACCATAC TGACCAGCCAGAATTTGCTACTGAGCTAGCCTGGGAGATTGACAGGAAAGTCCTTCATCAGCACCGGTTA CAGCGCACGCCGATCAAACTCCAGTGCTTTGCCTTGGACCCGCAGACTTCTGCCAAGGAAACCGTAGGCT ACATTGTTTTGGACTTAAGAACAGCTCAAGAAACAAAGCAGGCTCCCAAATGGTACCAGCTGCTGAGTAA CAAATACACCAAGTTCAAGGCCGAGGTGCAGATCAGCCTCACTTTGGAGACAGATACAAAGGCCCAGGTG GATAGTTATAAAGCAAAAGCAGCCCCGCCTCGGGATGGAAAAGTTCTTGCTAGCCTGGCAGGAGTTGACC CGAAGGACATTGTTGCAGTATTGAATGAGGAAGGAGGCTACCATCAGATTGGACCAGCGGAGCACTGTAC TGACCCCTTTATTTTGTCGGTGACGATTGCATTTGCCACCCAGTTGGAACAGTTAATTCCATGTACCATG AAGCTTCCAGAAAGGCAGCCTGAGTTTTTCTTTTACTATTCTTTACTGGGAAACGATGTTACAAACGAGC CTTTCAGTGATTTGATCAACCCAAACTTTGAGCCCGAGAGGGCATCTGTTCGTATACGCAGCAGTGTGGA GATTCTGCGTGTGTACCTGGCTCTTCACTCTAAACTGCAGATCCATCTCTGCTGTGGAGACCAGTCACTT GGCAGTACAGAAATACCTTTAAATGGATTACTGAAGAAGGGCAGTACAGAGATTAACCAGCATCCGGTCA CAGTAGAAGGTGCTTTTACCCTTGACCCTCCAAACCGAGCCAAGCAGAAGCTAGCTCCAGTCCCTCTGGA CCTGGCCCCAACTGTGGGCGTGTCCGTGGCTCTGCAGAGAGAAGGCATCGATTCCCAGTCTTTAATTGAA TTAAAGACCCAGAATGGACATGAGGCAGAACATTCACAGAAGAGAGTTTTAACCCCCATAAAGGAGAAGA CACTCACTGGGCCAAAATCACCAAGGGAGTCCCCTGCCCCGCCCCCACCTCCAAACCAGACGCCTCCAAC CAAGGATGATGCAACAGAAAGTGAAGTGGAGAGCCTGCAGTATGACAAGGATCCAAAGCCCACTGTGAAG GGCATCGGTTCTGTACCTGCTTCGCTGGCCCAGCCAGAGGCCACCTGTGGTGCGTCAGAAGTGGTGACAT CAGGACAGAAGATTGCTGTTCCAGCGGCGTCACACCACTTCTGCTTCTCAGTAGACTTAAGAAGCGTCCA TGACTTGGAACTCAGTTTTCCAGTCAACTGCATATTGAGGTACTCCTACCCATTCTTCGGCAGTGCAGCT CCTATTATGACTAACCCTCCCGTAGAAGTTCGGAAAAACATGGAGGTTTTTCTTCCTCAGTCTTACTGTG CGTTTGATTTTGCAACTATGCCTCACCAGCTCCAAGACACCTTCCTACGGATTCCTCTGCTGGTGGAGTT ATGGCACAAGGATAAAATGAGTAAAGATTTACTCCTTGGGGTCGCAAGAATCCAGCTTTCTAATATCTTG TCTTCAGAAAAGACCCGGTTTTTAGGTGCTAATGGTGAGCAGTGCTGGCGACAGACGTACAGTGAGAGCG TGCCTGTGATAGCAGCCCAGGGGTCAAATAACAGGATATTAGACCTTTCTTATACAATGACACTGGAAGA CTATGGCCTAGTGAAAATGCGTGAGATCTTTGTATCGGAGTCATCTCAGGGTGTGCCTGCTGTGGATCAG AAGCCATCTTCGCCTCCTCCCGCTCCCTGCCCTTCTGAGATCCAGATGGAACCCAGAGAGACCTTAGAGT ACAAGGCTGCCCTCGAGCTGGAGATGTGGAAAGAGATGCAGGAAGACATATTTGAAAGTCAGCTGAAGCA AAAAGAACTGGCTCACATGCAAGCTCTTGCAGAAGAGTGGAAAAAAAGGGACCGGGAAAGAGAGTCACTA GTGAAGAAAAAGGTAGCTGAATACAGTATTCTAGAAGGCAAACTTCAGAAGGCCCTCACGGAGCTAGAGA CCCGTGAGCAGCAGCTGGCCAGTGCAGAAGCAGAGCTTCAGAGAGAGAGAAAGGAGCTGCAGTTAGAACG GGAGCGGAACCTTCAAGAGCTGCAGGACTCTGTCCGAAGGGCCAGAGACGACTGTGTGTACCAAGTGGAG CTGGAGAGGCTGAAGCTGAAGCAGCTGGAAGAGGACAAACAGCGGCTTCAGCAGCAGCTTAATGATGCTG GCAATAAGTATAAGACTTTGGAAAAAGAGTTTCAGCAGTTCAAGGATCAACAGAACAACAAACCAGAAAT CCGGCTGCAGTCAGAAATAAACCTCCTCACCTTGGAGAAGGTTGAACTTGAAAGAAAGTTGGAATCTGCA ACCAAGTCTAAACTGCATTACAAGCAGCAGTGGGGCCGAGCTTTGAAAGAACTTGCCAGACTAAAGCAGA GGGAGCAAGAAAGTCAAATGGCCCGCCTTAAAAAACAGCAGGAGGAGTTGGAGCAGATGCGGCTGCGTTA CCTGGCGGCTGAGGAGAAAGAAACAGTGAGAACTGAGCAGCAGGAACTGCTGGATATAAGGAACGAGCTG AACAGGTTAAGGCAGCAAGAACAGAACCAGTACCAGGACTGCAAAGAGATTGCAAGTGGGAAGCTGGGCA GCCCGAGGGGCTCTGGGCTGGAAGAAGGCTTGGATGACTACCTGACACGCCTGATAGAGGAAAGGGACAC TCTGATGAGAACGGGTGTCTATAACCATGAGGACCGCATAATAAGTGAACTCGACCGGCAGATCAGAGAG GTTTTGACCAAAAACAGTGCCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211393 representing NM_178686
Red=Cloning site Green=Tags(s) MVPKSDQLLIVVSILEGRHFPKRPKHLLVVEAKFDGEQLATDPVDHTDQPEFATELAWEIDRKVLHQHRL QRTPIKLQCFALDPQTSAKETVGYIVLDLRTAQETKQAPKWYQLLSNKYTKFKAEVQISLTLETDTKAQV DSYKAKAAPPRDGKVLASLAGVDPKDIVAVLNEEGGYHQIGPAEHCTDPFILSVTIAFATQLEQLIPCTM KLPERQPEFFFYYSLLGNDVTNEPFSDLINPNFEPERASVRIRSSVEILRVYLALHSKLQIHLCCGDQSL GSTEIPLNGLLKKGSTEINQHPVTVEGAFTLDPPNRAKQKLAPVPLDLAPTVGVSVALQREGIDSQSLIE LKTQNGHEAEHSQKRVLTPIKEKTLTGPKSPRESPAPPPPPNQTPPTKDDATESEVESLQYDKDPKPTVK GIGSVPASLAQPEATCGASEVVTSGQKIAVPAASHHFCFSVDLRSVHDLELSFPVNCILRYSYPFFGSAA PIMTNPPVEVRKNMEVFLPQSYCAFDFATMPHQLQDTFLRIPLLVELWHKDKMSKDLLLGVARIQLSNIL SSEKTRFLGANGEQCWRQTYSESVPVIAAQGSNNRILDLSYTMTLEDYGLVKMREIFVSESSQGVPAVDQ KPSSPPPAPCPSEIQMEPRETLEYKAALELEMWKEMQEDIFESQLKQKELAHMQALAEEWKKRDRERESL VKKKVAEYSILEGKLQKALTELETREQQLASAEAELQRERKELQLERERNLQELQDSVRRARDDCVYQVE LERLKLKQLEEDKQRLQQQLNDAGNKYKTLEKEFQQFKDQQNNKPEIRLQSEINLLTLEKVELERKLESA TKSKLHYKQQWGRALKELARLKQREQESQMARLKKQQEELEQMRLRYLAAEEKETVRTEQQELLDIRNEL NRLRQQEQNQYQDCKEIASGKLGSPRGSGLEEGLDDYLTRLIEERDTLMRTGVYNHEDRIISELDRQIRE VLTKNSAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178686 |
| ORF Size | 2964 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178686.4 |
| RefSeq Size | 4572 bp |
| RefSeq ORF | 2967 bp |
| Locus ID | 225523 |
| UniProt ID | Q7TSG1 |
| MW | 112.6 kDa |
| Gene Summary | Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors and for proper positioning of neurons during brain development. Also implicated in the migration and selfrenewal of neural progenitors. Required for centriole duplication and maturation during mitosis and subsequent ciliogenesis. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203364 | Cep120 (untagged) - Mouse centrosomal protein 120 (Cep120), (10ug) |
CNY 7,240.00 |
|
| MG211393 | Cep120 (tGFP-tagged) - Mouse coiled-coil domain containing 100 (Ccdc100) |
CNY 9,500.00 |
|
| MR211393L3 | Lenti ORF clone of Cep120 (Myc-DDK-tagged) - Mouse centrosomal protein 120 (Cep120) |
CNY 10,550.00 |
|
| MR211393L4 | Lenti ORF clone of Cep120 (mGFP-tagged) - Mouse centrosomal protein 120 (Cep120) |
CNY 10,550.00 |
