Kcnh5 (NM_172805) Mouse Tagged ORF Clone

CAT#: MR211392

  • TrueORF®

Kcnh5 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 5 (Kcnh5)

ORF Plasmid: DDK tGFP



  "NM_172805" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,240.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Eag2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211392 representing NM_172805
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGGGGCAAGAGAGGGCTGGTGGCTCCGCAGAACACATTTTTGGAGAACATCGTCAGGCGCTCCA
GTGAATCAAGTTTCTTACTGGGAAATGCCCAAATTGTGGACTGGCCTGTAGTTTATAGTAATGACGGTTT
TTGTAAACTCTCTGGATATCATCGAGCTGACGTCATGCAGAAAAGCAGCACCTGCAGTTTTATGTATGGA
GAACTGACCGACAAGAAGACCATTGAGAAAGTCAGGCAGACTTTTGACAACTACGAGTCAAACTGTTTTG
AAGTTCTTCTGTACAAAAAAAACCGTACCCCTGTTTGGTTTTACATGCAAATCGCACCAATAAGAAATGA
ACACGAGAAGGTGGTCTTGTTCCTGTGTACATTCAAGGACATTACACTGTTCAAACAGCCTATAGAGGAT
GACTCAACGAAAGGTTGGACGAAGTTTGCCCGATTGACACGGGCTTTGACAAATAGCCGAAGTGTTTTAC
AGCAGCTTACACCAATGAATAAAACAGAGACAGTCCACAAGCATTCAAGACTAGCTGAAGTGCTTCAGCT
GGGATCAGACATCCTTCCTCAGTACAAACAAGAAGCGCCAAAGACGCCACCACATATCATCTTACACTAT
TGTGCTTTTAAAACTACTTGGGATTGGGTGATTTTAATTCTTACCTTCTACACCGCCATCATGGTTCCTT
ACAATGTTTCTTTCAAAACAAAGCAGAACAATATCGCCTGGCTGGTGCTGGACAGTGTGGTGGACGTGAT
TTTTCTGGTGGACATTGTTTTAAATTTTCACACGACTTTTGTGGGGCCGGGTGGAGAGGTCATTTCTGAC
CCGAAGCTTATAAGGATGAACTATCTGAAAACTTGGTTTGTGATTGATCTGCTGTCTTGTTTACCTTATG
ACATCATCAATGCCTTTGAAAATGTGGATGAGGGAATCAGCAGTCTCTTCAGCTCTTTAAAGGTGGTGCG
CCTTTTACGCCTGGGCCGTGTTGCTAGGAAACTGGACCATTACCTGGAATATGGAGCAGCGGTCCTTGTG
CTCCTGGTATGTGTGTTTGGACTGGTGGCTCACTGGCTGGCTTGCATCTGGTACAGCATTGGAGACTATG
AGGTCATTGATGAAGTCACCAACACCATTCAAATAGACAGCTGGCTCTACCAGTTGGCCTTGAGCATTGG
GACCCCATATCGCTACAATACGAGTGCAGGGATCTGGGAAGGAGGACCCAGCAAGGACTCCCTGTATGTG
TCCTCCCTCTACTTCACCATGACGAGCCTTACAACCATAGGATTTGGAAACATAGCTCCGACCACAGACG
TGGAGAAGATGTTCTCAGTGGCCATGATGATGGTCGGCTCTCTTCTTTATGCAACTATTTTTGGAAATGT
TACCACAATTTTCCAGCAAATGTATGCCAACACCAACCGTTACCATGAGATGCTGAATAATGTTCGAGAT
TTCCTGAAATTATATCAGGTCCCAAAAGGCCTTAGTGAACGAGTGATGGATTATATTGTCTCAACATGGT
CAATGTCAAAAGGCATCGACACAGAGAAGGTCCTCTCCATCTGTCCCAAGGACATGAGAGCTGACATCTG
TGTTCATCTGAACCGGAAGGTTTTTAATGAACATCCTGCATTCCGATTGGCCAGCGATGGATGTCTGCGT
GCCTTGGCAGTGGAGTTCCAAACCATTCACTGTGCTCCTGGGGACCTGATTTACCATGCTGGAGAGAGTG
TGGATGCCCTCTGCTTTGTGGTGTCCGGGTCCTTGGAAGTTATCCAGGATGAGGAGGTGGTGGCCATTTT
AGGGAAAGGTGACGTGTTTGGAGACATCTTCTGGAAGGAAACCACCCTTGCTCATGCGTGTGCCAATGTC
CGGGCCCTGACTTACTGTGATCTGCACATCATCAAGCGAGAAGCCTTGCTCAAGGTCCTTGACTTCTATA
CAGCATTTGCAAACTCATTTTCCAGGAACCTCACTCTCACTTGCAATCTAAGGAAGCGGATCATCTTCCG
TAAAATTAGTGATGTGAAGAAGGAGGAGGAGGAGCGGCTGCGGCAGAAGAATGAGGTGACTCTGAGCATC
CCCGTGGACCACCCAGTGAGGAAGCTCTTCCAGAAGTTCAAACAGCAGAAAGAACTGAGGATCCAGGGCT
CTGCACAGAGTGATCCTGAGAGGAGCCAGCTTCAGGTAGAGAGCCGCCCTTTGCAGAACGGAGCCTCCAT
CACAGGTACCAGTGTGGTCACCGTATCACAGATTACCCCCATCCAGACGTCTCTGGCGTATGTGAAAACC
AGCGAGTCTCTTAAGCAGAACAACCGCGATGCCATGGAACTGAAGCCCAATGGTGGCGCTGAACCCAAAT
GTCTCAAGGTCAACAGCCCCATCAGGATGAAAAACGGAAATGGGAAGGGGTGGCTGAGACTCAAGAACAA
CATGGGGGCTCAGGAGGAGAAAAAGGAAGACTGGAATAATGTCACCAAAGCCGAGTCGATGGGGCTCTTG
TCGGAGGACCCCAAGGGCAGTGACTCAGAGAACAGTGTGACCAAGAACCCCTTAAGGAAAACGGATTCTT
GTGACAGTGGGATTACAAAAAGTGACCTTCGTTTGGATAAAGCCGGTGAGGCCCGAAGTCCTCTAGAACA
CAGCCCCAGCCAGGCTGATGTCAAGCATTCTTTCTACCCCATCCCCGAGCAGGCCTTACAAACCACACTG
CAGGAAGTCAAACATGAACTCAAGGAGGATATCCAGCTGCTAAGCTGCAGAATGACTGCCCTGGAAAAGC
AGGTGGCAGAGATTTTAAAACTACTCTCAGAGAAAAGCGTACCCCAGACATCTTCTCCAAAACCCCAAAT
TCCACTCCAAGTACCTCCCCAAATACCATGTCAGGATATTTTTAGTGTCTCAAGGCCTGAGTCACCCGAA
TCTGACAAAGACGAAATCAACTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211392 representing NM_172805
Red=Cloning site Green=Tags(s)

MPGGKRGLVAPQNTFLENIVRRSSESSFLLGNAQIVDWPVVYSNDGFCKLSGYHRADVMQKSSTCSFMYG
ELTDKKTIEKVRQTFDNYESNCFEVLLYKKNRTPVWFYMQIAPIRNEHEKVVLFLCTFKDITLFKQPIED
DSTKGWTKFARLTRALTNSRSVLQQLTPMNKTETVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHY
CAFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNIAWLVLDSVVDVIFLVDIVLNFHTTFVGPGGEVISD
PKLIRMNYLKTWFVIDLLSCLPYDIINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYLEYGAAVLV
LLVCVFGLVAHWLACIWYSIGDYEVIDEVTNTIQIDSWLYQLALSIGTPYRYNTSAGIWEGGPSKDSLYV
SSLYFTMTSLTTIGFGNIAPTTDVEKMFSVAMMMVGSLLYATIFGNVTTIFQQMYANTNRYHEMLNNVRD
FLKLYQVPKGLSERVMDYIVSTWSMSKGIDTEKVLSICPKDMRADICVHLNRKVFNEHPAFRLASDGCLR
ALAVEFQTIHCAPGDLIYHAGESVDALCFVVSGSLEVIQDEEVVAILGKGDVFGDIFWKETTLAHACANV
RALTYCDLHIIKREALLKVLDFYTAFANSFSRNLTLTCNLRKRIIFRKISDVKKEEEERLRQKNEVTLSI
PVDHPVRKLFQKFKQQKELRIQGSAQSDPERSQLQVESRPLQNGASITGTSVVTVSQITPIQTSLAYVKT
SESLKQNNRDAMELKPNGGAEPKCLKVNSPIRMKNGNGKGWLRLKNNMGAQEEKKEDWNNVTKAESMGLL
SEDPKGSDSENSVTKNPLRKTDSCDSGITKSDLRLDKAGEARSPLEHSPSQADVKHSFYPIPEQALQTTL
QEVKHELKEDIQLLSCRMTALEKQVAEILKLLSEKSVPQTSSPKPQIPLQVPPQIPCQDIFSVSRPESPE
SDKDEINF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_172805
ORF Size 2964 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_172805.3, NP_766393.2
RefSeq Size 4061 bp
RefSeq ORF 2967 bp
Locus ID 238271
UniProt ID Q920E3
MW 112.3 kDa
Gene Summary Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current (By similarity). Channel properties may be modulated by cAMP and subunit assembly.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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