Epha1 (NM_023580) Mouse Tagged ORF Clone
CAT#: MR211365
- TrueORF®
Epha1 (Myc-DDK-tagged) - Mouse Eph receptor A1 (Epha1)
ORF Plasmid: tGFP
"NM_023580" in other vectors (4)
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CNY 7,160.00
CNY 8,550.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5730453L17Rik; AL033318; Eph; Esk |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211365 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCGCTGGCCCCTGGGGCTTGCATTGCTGCTGCTGCTGCTCTGCGCCCCGCTGCCCCCGGGGG CGCGCGCCGAGGAAGTCACTCTAATGGACACAAGCACAGCACAAGCAGAGCTGGGCTGGCTTCTGGATCC CCCAGAGACTGGGTGGAGTGAGGTGCAACAAATGCTAAACGGGACACCCCTGTACATGTACCAAGACTGC CCAATACAGGAAGGTGGGGACACTGACCACTGGCTTCGCTCCAATTGGATCTACCGCGGAGAGGAAGCTT CACGCATCTACGTGGAGCTGCAGTTCACCGTGCGGGACTGTAAGAGTTTCCCAGGGGGAGCTGGGCCTCT GGGATGCAAAGAGACCTTCAACCTTTTCTACATGGAGAGTGACCAGGATGTGGGCATTCAGCTCCGACGA CCTTTGTTCCAAAAGGTAACAACTGTGGCAGCAGACCAGAGCTTCACCATCAGAGACCTGGCATCTGGCT CTGTAAAGCTGAATGTAGAACGCTGCTCGTTGGGCCACCTCACCCGCCGTGGCCTCTACTTAGCTTTCCA CAACCCGGGTTCCTGTGTGGCGCTAGTGTCTGTAAGGGTGTTCTACCAGCGCTGTGCCGAGACCGTGCAT GGCTTGGCCCACTTCCCTGACACTCTCCCTGGACCTGGAGGGTTGGTTGAAGTAGCTGGAACGTGCCTCT CCCATGCACAGATCAGCTTGGGGTCCTCAGGTACACCACGAATGCACTGCAGCCCTGATGGCGAGTGGCT GGTGCCTGTGGGTCAGTGCCAGTGCGAGCCTGGCTATGAAGAAAGCAGTGGAAATGTGGGATGCACTGCC TGTCCTACTGGTTTCTATCGAGTGGACATGAATACACTCCGTTGTCTCAAGTGCCCCCAACATAGCATAG CAGAGTCTGAGGGGTCTACCATCTGTACCTGTGAGAATGGACATTATCGAGCCCCTGGGGAGGGTCCCCA GGTAGCATGCACACGTCCCCCATCGGCTCCCCAAAATCTGAGCTTCTCCACATCAGGGACTCAACTCTCC CTGCGCTGGGAGCCCCCCAGAGATACAGGGGGACGCCATGATATCAGATACAGCGTGGAGTGCTTGCAGT GTCGGGGCATTGCACAGGATGGGGGTCCCTGCCAACCCTGTGGAAAAGGTGTGCACTTTTCCCCGGCTGC TTCCGGGCTCACCACATCTACCGTGCAAGTGCAAGGCCTCGAGCCTTACGCCAACTACACATTTACCGTC AAATCCCAAAACAGAGTGTCAGGACTGGACAGTTCCAGCCCTAGCAGCGCCTCCCTCAGTATCAACATGG GGCACGCAGAGTCACTCTCTGGCCTGTCACTGAAGCTGGTGAAGAAAGAACCGAGGCAGCTGGAGCTGAC TTGGGCAGGGTCCCGACCCCGAAATCCTGGAGGGAATCTGAGCTATGAGCTGCACGTGCTGAATCAGGAC GAAGAATGGCACCAGATGGTGTTGGAACCCAGGGTCTTGCTGACAAAACTTCAGCCAGATACCACATACA TTGTCAGAGTGCGAACACTGACCCCACTGGGGCCTGGCCCTTTCTCCCCTGACCATGAGTTTCGGACAAG CCCACCAGTTTCCAGAAGCCTGACCGGAGGAGAGATTGTGGCCGTCATCTTTGGATTGCTGCTTGGAATA GCTCTGCTGATCGGGATTTATGTCTTCCGTTCAAGGAGAGGCCAGAGACAGAGACAGCAGAGGCAGCGTG AACGCACCACCAATGTCGATCGAGAGGACAAGCTGTGGCTAAAACCCTATGTGGACCTCCAGGCCTATGA GGACCCTGCACAGGGAGCCTTAGACTTTGCCCAGGAACTGGACCCAGCCTGGCTGATTGTGGACACTGTC ATAGGAGAAGGGGAGTTTGGTGAAGTGTATCGGGGAGCCCTGAGACTCCCCAGCCAAGATTGCAAGACTG TGGCCATTAAGACCTTGAAAGATACATCCCCAGATGGCTACTGGTGGAATTTCCTTCGAGAGGCAACTAT CATGGGCCAGTTCAACCACCCACACATTCTACGCCTAGAAGGTGTCATCACAAAAAGAAAGCCCATCATG ATCATCACAGAATTTATGGAAAATGGAGCCCTGGATGCCTTTCTGAAGGAACGGGAGGACCAACTAGCTC CTGGTCAGCTAGTGGCTATGCTACTGGGCATAGCATCAGGCATGAACTGCCTCAGTGGCCACAATTATGT CCATAGAGACCTGGCTGCCAGGAACATCTTGGTGAATCAGAACCTGTGCTGCAAGGTGTCTGACTTTGGC TTGACCCGCCTCCTGGATGACTTTGACGGCACCTATGAAACCCAGGGAGGAAAGATCCCCATCCGATGGA CAGCCCCAGAAGCTATTGCCCATCGGATCTTCACCACAGCCAGTGATGTGTGGAGCTTTGGGATTGTAAT GTGGGAGGTGTTGAGTTTTGGCGACAAACCCTATGGGGAGATGAGCAACCAAGAGGTAATGAAAAGCATT GAAGATGGGTACCGGTTGCCCCCTCCTGTGGACTGTCCTGCCCCTCTCTATGAACTCATGAAGAACTGCT GGGCTTACGATCGTGCCCGTCGACCCCACTTCCTCCAGCTGCAGGCACATCTGGAACAGTTGCTTACTGA CCCCCATTCCCTAAGGACAATTGCCAACTTTGACCCTAGGGTGACCTTACGCCTGCCCAGCCTGAGTGGC TCTGATGGGATCCCTTATCGAAGTGTCTCTGAGTGGCTTGAATCCATACGCATGAAGCGCTACATCCTGC ACTTCCGTTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGACGGCTGAGGACCTGACGCAGAT GGGAATAACGTTGCCAGGGCACCAGAAACGAATTCTCTGCAGTATTCAAGGATTTAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211365 protein sequence
Red=Cloning site Green=Tags(s) MERRWPLGLALLLLLLCAPLPPGARAEEVTLMDTSTAQAELGWLLDPPETGWSEVQQMLNGTPLYMYQDC PIQEGGDTDHWLRSNWIYRGEEASRIYVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRR PLFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQRCAETVH GLAHFPDTLPGPGGLVEVAGTCLSHAQISLGSSGTPRMHCSPDGEWLVPVGQCQCEPGYEESSGNVGCTA CPTGFYRVDMNTLRCLKCPQHSIAESEGSTICTCENGHYRAPGEGPQVACTRPPSAPQNLSFSTSGTQLS LRWEPPRDTGGRHDIRYSVECLQCRGIAQDGGPCQPCGKGVHFSPAASGLTTSTVQVQGLEPYANYTFTV KSQNRVSGLDSSSPSSASLSINMGHAESLSGLSLKLVKKEPRQLELTWAGSRPRNPGGNLSYELHVLNQD EEWHQMVLEPRVLLTKLQPDTTYIVRVRTLTPLGPGPFSPDHEFRTSPPVSRSLTGGEIVAVIFGLLLGI ALLIGIYVFRSRRGQRQRQQRQRERTTNVDREDKLWLKPYVDLQAYEDPAQGALDFAQELDPAWLIVDTV IGEGEFGEVYRGALRLPSQDCKTVAIKTLKDTSPDGYWWNFLREATIMGQFNHPHILRLEGVITKRKPIM IITEFMENGALDAFLKEREDQLAPGQLVAMLLGIASGMNCLSGHNYVHRDLAARNILVNQNLCCKVSDFG LTRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSI EDGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFLQLQAHLEQLLTDPHSLRTIANFDPRVTLRLPSLSG SDGIPYRSVSEWLESIRMKRYILHFRSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023580 |
ORF Size | 2934 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023580.1, NM_023580.2, NM_023580.3, NM_023580.4, NP_076069.2 |
RefSeq Size | 3273 bp |
RefSeq ORF | 2934 bp |
Locus ID | 13835 |
UniProt ID | Q60750 |
MW | 108.6 kDa |
Gene Summary | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition
,Yang, Y;Zhou, C;Wang, Y;Liu, W;Liu, C;Wang, L;Liu, Y;Shang, Y;Li, M;Zhou, S;Wang, Y;Zeng, W;Zhou, J;Huo, R;Li, W;,
EMBO Rep
,PubMed ID 28073917
[Epha1]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205994 | Epha1 (untagged) - Mouse Eph receptor A1 (Epha1), (10ug) |
CNY 7,160.00 |
|
MG211365 | Epha1 (tGFP-tagged) - Mouse Eph receptor A1 (Epha1) |
CNY 9,410.00 |
|
MR211365L3 | Lenti ORF clone of Epha1 (Myc-DDK-tagged) - Mouse Eph receptor A1 (Epha1) |
CNY 10,450.00 |
|
MR211365L4 | Lenti ORF clone of Epha1 (mGFP-tagged) - Mouse Eph receptor A1 (Epha1) |
CNY 10,450.00 |