Vps54 (BC025012) Mouse Tagged ORF Clone

CAT#: MR211333

  • TrueORF®

Vps54 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 54 (yeast) (cDNA clone MGC:32446 IMAGE:5042879)

ORF Plasmid: DDK tGFP



  "BC025012" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 9,600.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Vps54l, Hcc8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211333 representing BC025012
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCAAGCCACAGTTCTTCTCCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAAAAAGAGG
TAGATCCAACAAAACACATTCGACCTGTGCAGTCACTGCCAGATGTGTGTCCCAAGGAACCCACAGTTAC
AGATCAACATAGATGGACTGTGTATCATTCCAAAGTAAATCTCCCAGCAGCATTAAATGATCCTACACTA
GCAAAAAGAGAATCTGACTTCTTCACAAAAACTTGGGGATTGGACTTTGTGGACACTGAAGTCATACCTT
CACTCTACCTCCCACAGATCAGCAAGGAGAATTTTATAGCATATCAGCAGGAGATCTCTCAGAGAGAGAA
GATTCATGAGAGATGCAAGAATATTTGTCCTCCTAAAGATACCTTTGACAGGACTCTTTTACATATTCAT
GATAAATCCAGGACAGATCTGGAGCAAGTACCTAAGATTTTTATGAAACCAGATTTTGCCTTGGATGATT
CTTTAACTTTTAATTCAGTTTTACCATGGTCTCATTTTAATACTGCTGGTGGGAAAGGAAGTCGCGACGC
AGCTTCTTCCAAGTTGCTTCAGGAAAAGCTGAGCCATTATCTGGATATTGTGGAAGTAAACATTGCTCAC
CAGATCTCTCTACGTTCAGAAGCATTTTTTCATGCAATGACCTCTCAACACGAATTGCAGGACTACCTCA
AGAAAACTACCCAGGCTGTAAAAATGCTTCGAGATAAAATTGCACAGATTGATAAAGTTATGTGTGAAGG
ATCACTACAGATTTTAAGACTGGCACTTACCAGAAATAATTGTGTTAAAGTATACAATAAACTGAAGTTA
ATGGCCACTGTACACCAAACTCAGCCTACAGTGCAGGTGTTGTTATCTACTTCTGAATTTGTTGGAGCGT
TGGACTTAATAGCAACAACTCAAGAGGTTCTGCAGCAGGAGCTTCAGGGCATTCACAGCTTCCGGCATTT
GGGATCACAGCTTTGTGAATTAGAAAAGCTGATTGATAAAATGATGATTGCAGAATTTTCTACTTATTCT
CATAGTGACTTAAATAGACCACTGGAAGGTGAATGCCAAGTTTTAGAAGAGGAAAGATTAGTATCTCTTG
TATTTGGGCTTTTGAAACAAAGAAAACTTAATTTTTTAGAAATCTATGGTGAAGAAATGATTATTACAGC
AAAGAACATCATTAAAGAGCGTGTGATTAATAAAGTTTCACAAATAGAAGAAATAGACACAGACGTTGTT
GTAAAGCTTGCAGATCAAATGAGAATGTTGAATTTTCCCCAGTGGATTGATCTGTTAAAGGACATTTTCT
CTAAGTTTACAGTTTTCCTACAGAGAGTTAAGGCAACATTGAATATTATCCACAGTGTTGTTCTGTCAGT
TCTTGAGAAAAGCCAAAGGACTAGAGAATTGGAAGAGATCCCACAGCAGAGGAGCGCTGGGAAGGACAGC
TCCTTGGACACAGACGTGGCTTACCTGACACACGAGGGCTGGTTCATAAGTGATGCTTTCAGTGAGGGTG
AGCCAGCATCTGCGGCGGTTGATACTACCTCTCAAAGAAACACGTCTCCACATAGCGAGCCCTGCAGCAG
TGATTCGGTATCAGAACCAGAATGTACTACTGATTCTTCATCCAGCAAGGAGCAGACATCAGCGTGTGCT
CCTCCAGGAGGTATAGAGATTATAGTCAGTGAAGACATGAGGTTAACTGACTTGGAGCTAGGAAAACTAG
CAAGTAACATCCAGGAGTTACTGTGTAATGCTTCAGATGTATGCCATGATCGAGCTGTCAAATTTCTCAT
GTCAAGAGCAAAGGACGGTTTTCTTGAAAAATTGAATTCCACGGAATTCATAGCACTCTCGAGATTAATG
GAGACATTCATAGTGGACACTGAACAGATCTGTGGGAGGAAGAGCACGTCACTGCTTGGGGCCCTTCAGA
GCCAAGCAAATAAGTTTGTGAACAGGTTTCATGAAGAGAGGCGGACCAAGCTCAGCCTCCTCTTAGACAA
CGAGCGTTGGAAGCAGGCTGATGTTCCTGCAGAATTTCAGGATCTTGTTGATTCTATAGCGGATGGAAAG
ATTGCTTTACCTGAAAAAAAGCCAGTGGTTACAGAAGAAAGGAAGCCAGCTGATGTTCTTGTTGTTGAGG
GACACCAGTATGCAGTTGTCGGAACCGTCTTGCTGTTAATAAGAATCATTCTTGAATATTGCCAGTGTGT
GGATAACATCCCCTCCGTCACTACTGACATGCTCACTCGCCTGACAGATCTGCTGAAGTACTTCAATTCA
AGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCTTTGCAAGTTGTTGGACTAAAAACAATTACTACAAAAA
ACTTGGCCCTCTCTTCACGGTGTCTGCAGTTGATTGTGCACTACATTCCCGTGATCCGGGCTCACTTTGA
AGCCCGACTGCCCCCCAAGCAATGGAGCATGCTTAGACATTTTGATCACATCACAAAGGATTACCATGAT
CATATAGCTGAAATTTCAGCTAAGCTTGTAGCGATAATGGATAGCCTATTTGATAAGCTGTTATCAAAGT
ATGAGGTGAAAGCTCCTGTTCCTTCCCCGTGTTTCAGGAATATTTGTAAGCAAATGACAAAAATGCATGA
AGCTATATTTGATCTTCTTCCCGAAGAACAAACACAGATGTTATTTTTAAGAATTAATGCAAGTTACAAA
CTCCACTTGAAAAAACAGTTATCACACTTAAATGTAATAAATGACGGAGGACCTCAAAATGGGTTGGTCA
CAGCAGATGTAGCTTTTTACACTGGAAATCTTCAAGCCTTAAAAGGCCTTAAAGATCTGGATCTAAATAT
GGCCGAGATCTGGGAACAGAAGAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211333 representing BC025012
Red=Cloning site Green=Tags(s)

MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTVTDQHRWTVYHSKVNLPAALNDPTL
AKRESDFFTKTWGLDFVDTEVIPSLYLPQISKENFIAYQQEISQREKIHERCKNICPPKDTFDRTLLHIH
DKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAH
QISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKL
MATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYS
HSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVV
VKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDS
SLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACA
PPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLM
ETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGK
IALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNS
RSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHD
HIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYK
LHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC025012
ORF Size 2895 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC025012.2
RefSeq Size 3405 bp
RefSeq ORF 2897 bp
Locus ID 245944
MW 124.9 kDa
Gene Summary Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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